Received June 19, 1964.
... nucleotides by the pathway proposed in the Introduction. The observed greater conversion of orotic acid than either aspartic or carbamyl aspartic acids to -RNA is predicted by its position in the metabolic sequence, although comparisons are somewhat difficult because only the DL-forms of the latter ...
... nucleotides by the pathway proposed in the Introduction. The observed greater conversion of orotic acid than either aspartic or carbamyl aspartic acids to -RNA is predicted by its position in the metabolic sequence, although comparisons are somewhat difficult because only the DL-forms of the latter ...
Regulation of Stage I1 of Sporulation in Bacillus subtilis
... become apparent that most of them, possibly all, are expressed during vegetative growth (Losick et al., 1986; Yamashita et al., 1986) and therefore can be disregarded for our purposes. Sporulation from the developmental point of view can therefore be considered as beginning at stage 11. This begins ...
... become apparent that most of them, possibly all, are expressed during vegetative growth (Losick et al., 1986; Yamashita et al., 1986) and therefore can be disregarded for our purposes. Sporulation from the developmental point of view can therefore be considered as beginning at stage 11. This begins ...
EPICENTRE Enzyme Catalog
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
Journal Club Pack - Circulation Research
... Rationale: We previously identified conserved G/C Repressor elements in the promoters of most smooth muscle cell (SMC) marker genes and demonstrated that mutation of this element within the SM22α promoter nearly abrogated repression of this transgene after vascular wire injury or within lesions of A ...
... Rationale: We previously identified conserved G/C Repressor elements in the promoters of most smooth muscle cell (SMC) marker genes and demonstrated that mutation of this element within the SM22α promoter nearly abrogated repression of this transgene after vascular wire injury or within lesions of A ...
Bayesian Learning of MicroRNA Targets from Sequence
... Despite rapid advances in discovering and characterizing miRNAs, significant questions remain. One major challenge is determining the complete set of functional miRNA targets on a genome-wide scale. Previous work has focused primarily on the genome-wide computational discovery of miRNA genes [6, 17] ...
... Despite rapid advances in discovering and characterizing miRNAs, significant questions remain. One major challenge is determining the complete set of functional miRNA targets on a genome-wide scale. Previous work has focused primarily on the genome-wide computational discovery of miRNA genes [6, 17] ...
1 Oviduct-embryo interactions in cattle
... There is clear evidence of a two-way interaction between the uterus and developing conceptus. For example, it has been well demonstrated that circulating progesterone (P4) directly regulates uterine gene expression that, in turn, drives conceptus elongation [2, 3]. Up to the time of maternal recogni ...
... There is clear evidence of a two-way interaction between the uterus and developing conceptus. For example, it has been well demonstrated that circulating progesterone (P4) directly regulates uterine gene expression that, in turn, drives conceptus elongation [2, 3]. Up to the time of maternal recogni ...
Glutathione S-transferase copy number variation alters lung gene expression M.W. Butler*
... and adenocarcinoma, the most common smokingassociated lung cancer [5–15]. In addition to the small airways, the alveoli are also exposed to the stress of smoking [9, 10, 16, 17]. The alveoli are protected from xenobiotics, in part, by alveolar macrophages (AMs), the lung representative of the mononu ...
... and adenocarcinoma, the most common smokingassociated lung cancer [5–15]. In addition to the small airways, the alveoli are also exposed to the stress of smoking [9, 10, 16, 17]. The alveoli are protected from xenobiotics, in part, by alveolar macrophages (AMs), the lung representative of the mononu ...
Genome-wide identification and analysis of the SGR
... where it is bound in CHL-protein complexes (Markwell et al., 1979). The protein components of the two photosystems are structurally organized into morphologically distinct membrane subunits (Arntzen, 1978), including photosystem I (PSI) and photosystem II (PSII) reaction center complexes (Kusaba et ...
... where it is bound in CHL-protein complexes (Markwell et al., 1979). The protein components of the two photosystems are structurally organized into morphologically distinct membrane subunits (Arntzen, 1978), including photosystem I (PSI) and photosystem II (PSII) reaction center complexes (Kusaba et ...
PowerCut™ Dicer
... that is needed to completely cleave 1 µg of 192 bp doublestranded RNA substrate to siRNA in 16 hours at 37°C. Exonuclease contamination assay: Incubation of 1 U of PowerCut Dicer (4 h, 37°C, 50 μl) with 1 μg of sonicated [3H]-DNA (3x105 cpm/μg) in the assay buffer released <0.5 % of radioactivity. E ...
... that is needed to completely cleave 1 µg of 192 bp doublestranded RNA substrate to siRNA in 16 hours at 37°C. Exonuclease contamination assay: Incubation of 1 U of PowerCut Dicer (4 h, 37°C, 50 μl) with 1 μg of sonicated [3H]-DNA (3x105 cpm/μg) in the assay buffer released <0.5 % of radioactivity. E ...
ppt for
... • Small RNAs: piRNA, siRNA, miRNA. • piRNA: derive from repetitive genomic element, interact with PIWI AGO family protein ...
... • Small RNAs: piRNA, siRNA, miRNA. • piRNA: derive from repetitive genomic element, interact with PIWI AGO family protein ...
PDF
... The H19 gene was cloned 20 years ago (Pachnis et al., 1988) and was one of the first imprinted genes to be identified. Genomic imprinting is an epigenetic mechanism that leads to parent-of-origin specific monoallelic expression. Thus, H19 is expressed from the maternal allele in mouse and human (Bar ...
... The H19 gene was cloned 20 years ago (Pachnis et al., 1988) and was one of the first imprinted genes to be identified. Genomic imprinting is an epigenetic mechanism that leads to parent-of-origin specific monoallelic expression. Thus, H19 is expressed from the maternal allele in mouse and human (Bar ...
Complete Thyroxine-Binding Globulin (TBG) Deficiency Produced
... creates a new acceptor splice site one nucleotide upstream of the authentic acceptor splice site adding an intronic G to the downstream exon. This is in agreement with the majority of acceptor splice site mutations reported (15, 18). Exonic and intronic recognition sequences have an established role ...
... creates a new acceptor splice site one nucleotide upstream of the authentic acceptor splice site adding an intronic G to the downstream exon. This is in agreement with the majority of acceptor splice site mutations reported (15, 18). Exonic and intronic recognition sequences have an established role ...
The Chloroplast trnT–trnF Region in the Seed Plant
... based on results of a phylogenetic analysis of Gnetales rbcL and matK sequences (Won and Renner 2003). We used PAUP* 4.0b10 (Swofford 2002) and Mr. Bayes (version 3.0b4 [Huelsenbeck and Ronquist 2001]) for phylogenetic analyses. The data matrix of the trnL intron and trnT–trnL and trnL–trnF spacers w ...
... based on results of a phylogenetic analysis of Gnetales rbcL and matK sequences (Won and Renner 2003). We used PAUP* 4.0b10 (Swofford 2002) and Mr. Bayes (version 3.0b4 [Huelsenbeck and Ronquist 2001]) for phylogenetic analyses. The data matrix of the trnL intron and trnT–trnL and trnL–trnF spacers w ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial major outer membrane protein gene, ompl, occurs over the time course of the developmental cycle: transcrip ...
... Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial major outer membrane protein gene, ompl, occurs over the time course of the developmental cycle: transcrip ...
Induction of XIST expression from the human active
... The transcriptionally silent allele of XIST on the active X chromosome has been shown to be methylated at a series of restriction enzyme sites near the 5′ end of the gene (22). We used a PCR-based assay to examine methylation at a subset of these sites (Fig. 2). Primers AT-2 and 29r amplify DNA from ...
... The transcriptionally silent allele of XIST on the active X chromosome has been shown to be methylated at a series of restriction enzyme sites near the 5′ end of the gene (22). We used a PCR-based assay to examine methylation at a subset of these sites (Fig. 2). Primers AT-2 and 29r amplify DNA from ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
Lesson Plan, GeneChip® Microarrays: Teacher`s Guide
... synthesize concepts from previous topics in this course. These answers are in no way complete, but do make sure to get to the major points of the question. Part I – Intro, and Gene Expression Microarrays (1) What is gene expression? What can affect gene expression? ...
... synthesize concepts from previous topics in this course. These answers are in no way complete, but do make sure to get to the major points of the question. Part I – Intro, and Gene Expression Microarrays (1) What is gene expression? What can affect gene expression? ...
Genome-wide histone modification patterns in
... of H3K4me3 or H3K36me3 relative to their transcript abundance, we found that cell cycle genes in K. lactis, but not in S. cerevisiae, exhibit higher histone methylation than expected given their mRNA abundance (not shown). This potentially reflects differences in cell cycle kinetics between these sp ...
... of H3K4me3 or H3K36me3 relative to their transcript abundance, we found that cell cycle genes in K. lactis, but not in S. cerevisiae, exhibit higher histone methylation than expected given their mRNA abundance (not shown). This potentially reflects differences in cell cycle kinetics between these sp ...
Gene silencing in mammalian cells and the spread of DNA
... been shown that altered expression of a variety of chromatin modifying proteins (Wakimoto, 1998; Weiler and Wakimoto, 1995) can influence the percentage of red versus white cells in a given eye. Cell to cell variation has also been shown in the mouse, perhaps best visualized as variegated coat color ...
... been shown that altered expression of a variety of chromatin modifying proteins (Wakimoto, 1998; Weiler and Wakimoto, 1995) can influence the percentage of red versus white cells in a given eye. Cell to cell variation has also been shown in the mouse, perhaps best visualized as variegated coat color ...
Expression of phosphofructokinase in Neisseria meningitidis
... thus function in both glycolysis and gluconeogenesis. PPiPFK (EC 2.7.1.90) has a more limited distribution, but is also found in eukaryotes and bacteria (Bapteste et al., 2003). ATP-PFK and PPi-PFK share a common ancestry, but phylogenies show a very complex evolutionary pattern (Muller et al., 2001 ...
... thus function in both glycolysis and gluconeogenesis. PPiPFK (EC 2.7.1.90) has a more limited distribution, but is also found in eukaryotes and bacteria (Bapteste et al., 2003). ATP-PFK and PPi-PFK share a common ancestry, but phylogenies show a very complex evolutionary pattern (Muller et al., 2001 ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... are utilized as positive ligation controls and six or eight are utilized as negative controls. For each positive control, a separate ERCC RNA sequence of similar size to a miRNA is supplied. Six different small RNA controls are provided such that after dilution and addition to the miRNA sample prepa ...
... are utilized as positive ligation controls and six or eight are utilized as negative controls. For each positive control, a separate ERCC RNA sequence of similar size to a miRNA is supplied. Six different small RNA controls are provided such that after dilution and addition to the miRNA sample prepa ...
DNA sequencing revealed a definitive
... and their flanking regions. Where data is available, it can be seen that the amino acid sequences preceding the homeo domains are also similar. The sequence of 8 aa immediately preceding the HB4 homeo domain is highly similar to those immediately preceding the Hox 1.7 and XlHbox 6 homeo domains. Whe ...
... and their flanking regions. Where data is available, it can be seen that the amino acid sequences preceding the homeo domains are also similar. The sequence of 8 aa immediately preceding the HB4 homeo domain is highly similar to those immediately preceding the Hox 1.7 and XlHbox 6 homeo domains. Whe ...
Choosing the Right Centrifuge for Your - Laboratory
... informed decision. There is a good chance that your needs have changed since the last time you purchased a centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new prot ...
... informed decision. There is a good chance that your needs have changed since the last time you purchased a centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new prot ...