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A Twist-like bHLH gene is a downstream factor of an
A Twist-like bHLH gene is a downstream factor of an

... 2002). In the present study, we use the term ‘mesenchyme’ for all of these cells, except where indicated otherwise. In addition to these three lineages of mesenchymal cells, ascidian larvae develop trunk ventral cells (TVCs), which are often regarded as a kind of mesenchymal cell because they give r ...
CHAPTER 15
CHAPTER 15

... Mendel’s dihybrid cross experiments produced offspring that had a combination of traits that did not match either parent in the P generation.  If the P generation consists of a yellow-round seed parent (YYRR) crossed with a greenwrinkled seed parent (yyrr), all F1 plants have yellow-round seeds (Yy ...
Comparative study of overlapping genes in bacteria, with special
Comparative study of overlapping genes in bacteria, with special

... fact that Mycobacterium leprae has the maximum number of pseudogenes (>1000) compared to only 12 pseudogenes in R. prowazekii, the obligatory intracellular parasite with the most extensive genome degradation (Fig. 1). These observations clearly implicate the role of overlapping genes and their contr ...
Promega Notes: Technically Speaking: Tips for Working with RNA
Promega Notes: Technically Speaking: Tips for Working with RNA

... RNase inhibitors (e.g., RNasin® Ribonuclease Inhibitor) are often used in enzyme reactions to sequester RNases that may remain in an RNA sample or that may be introduced with other reagents or while pipetting. A number of valuable total RNA extraction precautions and troubleshooting concepts are pre ...
A plant dialect of the histone language
A plant dialect of the histone language

... HMT family [22]. Histone methylation currently focuses considerable research interest because it is associated with transcriptional regulation and methyl transferases contain well conserved domains among different organisms. In contrast to other modifications, no histone demethylases have thus far b ...
X - My Teacher Site
X - My Teacher Site

... 2) Neither Tim nor Rhoda has Duchenne muscular dystrophy, but their firstborn son does have it. What is the probability that a second child of this couple will have the disease? What is the probability if the second child is a ...
Identification of a Class of Chromatin Boundary Elements
Identification of a Class of Chromatin Boundary Elements

... 340-bp fragment from the gypsy retrotransposon (11). The scs/scs9 and the gypsy-derived elements have a boundary function in both of the assays mentioned above. They confer position-independent expression on a bracketed reporter gene by insulating the transgene from both activating and repressive ef ...
Transposable elements, genes and recombination in a 215
Transposable elements, genes and recombination in a 215

... a genome designated Am that is closely related to the genome of T. urartu, the A genome donor for tetraploid and hexaploid wheats. The large genome of T. monococcum (1C =5600 Mb; Bennett and Leitch 1995) is approximately 12 times larger than the genome of rice and 40 times larger than the genome of ...
Does Activation of the TALl Gene Occur in a Majority
Does Activation of the TALl Gene Occur in a Majority

... in these patients may result from subtle alterations in cisacting regulatorysequences (affecting expressionof a single TALT allele) or changes in transacting factors that control TALT transcription(affectingexpression of both TALTal- ...
Elke Winterhager (Ed.) Gap Junctions in Development
Elke Winterhager (Ed.) Gap Junctions in Development

... be mediated via protein–protein interactions at the C-terminus of the connexin. Thus, there is a need to discriminate between channel and protein function. Impairment of development and diseases need not be based on connexin mutations, but rather on wrong signaling mediated via protein– protein inte ...
Replicational and transcriptional selection on codon usage in
Replicational and transcriptional selection on codon usage in

... (7) and also M. pneumoniae (20). These organisms all seem to be benefiting from a mechanism of genome organization that maintains genes on the leading strand of replication. It is unlikely that this effect is a result of random genetic drift, because there seems to be an obvious selective advantage ...
Enolase and Glycolytic Flux Play a Role in the Regulation
Enolase and Glycolytic Flux Play a Role in the Regulation

Functional Annotation of Regulatory Pathways
Functional Annotation of Regulatory Pathways

... They also report that many transcriptional regulators within a functional category bind to transcriptional regulators that play key roles in the control of other cellular processes. For example, cell cycle activators are observed to bind to genes that are responsible for regulation of metabolism, en ...
Alterations in gene expression in T1α null lung: a model of deficient
Alterations in gene expression in T1α null lung: a model of deficient

... We used EASE bioinformatic tool to identify over-represented biological processes, defined by the Gene Ontology database (GO), for 239 genes that have altered expression at E18.5 and for the 50 genes altered at term in the absence of T1α (data not shown). Genes involved in vasculogenesis, protein ta ...
Recruitment of 5! Hoxa genes in the allantois is
Recruitment of 5! Hoxa genes in the allantois is

... reporter. H19 insulators are located at both extremities of the transgenes. All transgenic embryos were generated by pronuclear injection. ...
Changes in DNA-binding activity of transcription factors in the
Changes in DNA-binding activity of transcription factors in the

... Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrop ...
Why we have (only) five fingers per hand: Hox genes
Why we have (only) five fingers per hand: Hox genes

... of vertebrates and insects. In Drosophila these genes do not break the animal into developmental units (such as segments) but rather define the identity of each unit. A different set of genes is responsible for setting up the number of repeating segments in the developing insect (gap genes, pair-rul ...
here - FasterDB
here - FasterDB

... nucleotides of its intronic neighborhood on both sides, motifs corresponding to PTB binding sites are represented by yellow lines. The sequence of each exon can be retrieved by clicking on it. Furthermore, clicking on “Motifs” in the blue panel, allows displaying exact position of each motif and its ...
pSAT vectors: a modular series of plasmids for autofluorescent
pSAT vectors: a modular series of plasmids for autofluorescent

... 35S terminator and producing pSAT6-nosPnosT-EGFP-C1. A DNA fragment containing three copies of the Gal4 UAS and the TATA box from the 35S promoter were cloned by triple ligation of AgeI-EcoRI and MfeI-NcoI PCR fragments, respectively, from pTA7001 (AoyamaandChua, 1997) into the AgeI-NcoI sites of pS ...
PDF
PDF

... regulatory core protein to exert ‘organizer‘ activity in Xenopus embryos. Its ancient paralog, lhx3 (lim3), is expressed around the blastopore in amphioxus and ascidian, but not vertebrate, gastrulae. These two genes are thus implicated in organizer evolution, and we addressed the evolutionary origi ...
Document
Document

... • Single CpG methylation model – distinct DNA elements [usually transcription factor (TF)-binding sites] whose methylation or demethylation usually leads to gene silencing or activation ...
Glutathione S-transferase copy number variation alters lung gene expression M.W. Butler*
Glutathione S-transferase copy number variation alters lung gene expression M.W. Butler*

... Labeling Kit, followed by additional clean-up and quantification of the biotin-labelled copy (c)RNA yield using a spectrophotometer (all reagents from Affymetrix). In accordance with Affymetrix protocols, the test microarrays were first hybridised and, if quality control was acceptable, hybridisatio ...
Genetic epidemiology: Systemic lupus erythematosus | Arthritis
Genetic epidemiology: Systemic lupus erythematosus | Arthritis

... The traditional approach for locating a disease gene in humans is linkage analysis. Results from mouse models of SLE presented the first evidence for genetic linkage to an area of chromosome 1 in the mouse that is syntenic to human chromosome 1q23–42. In 1997, Tsao et al [14] published linkage evide ...
Effects of Nicotine on Embryological Neural Development A Senior
Effects of Nicotine on Embryological Neural Development A Senior

... and visceral motor neurons. As there is no known mechanism for how nicotine might affect motor neurons, it is possible that Islet-1, whose expression is upstream of other LIM homeobox genes in development of motor neurons, is the key component of the differentiation pathway affected. In order to asc ...
NIH Public Access
NIH Public Access

... repeats (identified as repeat A, B, C, D). Studies of Xist RNA transgenes in mouse ES cells suggest that the A-repeat region is required for Xist RNA to silence chromatin (Wutz et al., 2002) and is important for PRC2 (EZH2) recruitment to enact H3K27 methylation (Zhao et al., 2008). With respect to ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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