The Drosophila Gene Disruption Project: Progress
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
lecture - Berkeley MCB
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
... regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have sequences for them • Even when a ...
Incomplete penetrance of MHC susceptibility genes
... population and analyzed for the frequency-distributions of MHC al- ...
... population and analyzed for the frequency-distributions of MHC al- ...
Adrenomedullin Gene Expression Is Developmentally Regulated and
... was used as the DNA template for PCR mutagenesis to produce mutated Adm promoter constructs, which lack one (pMutA or pMutB) or both of the potential HIF-1 binding sites (pMutAB). In pMutA, the core HIF-1 site (59-ACGT-39) at -1095 upstream of the transcription binding site was replaced with 59-AAAT ...
... was used as the DNA template for PCR mutagenesis to produce mutated Adm promoter constructs, which lack one (pMutA or pMutB) or both of the potential HIF-1 binding sites (pMutAB). In pMutA, the core HIF-1 site (59-ACGT-39) at -1095 upstream of the transcription binding site was replaced with 59-AAAT ...
Genome Biology and
... • Confirmed local order and orientation of the sequences • Corrected artefactual duplications resulting from mixups • Verified most of the sequence with – BAC cloned overlap sequence, paired end sequence reads from fosmids, draft chimpanzee genome sequence Reprinted from: International Human Genome ...
... • Confirmed local order and orientation of the sequences • Corrected artefactual duplications resulting from mixups • Verified most of the sequence with – BAC cloned overlap sequence, paired end sequence reads from fosmids, draft chimpanzee genome sequence Reprinted from: International Human Genome ...
PDF
... The differentiated features of postmitotic neurons are dictated by the expression of specific transcription factors. The mechanisms by which the precise spatiotemporal expression patterns of these factors are regulated are poorly understood. In C. elegans, the ceh-36 Otx homeobox gene is expressed i ...
... The differentiated features of postmitotic neurons are dictated by the expression of specific transcription factors. The mechanisms by which the precise spatiotemporal expression patterns of these factors are regulated are poorly understood. In C. elegans, the ceh-36 Otx homeobox gene is expressed i ...
Author`s personal copy - Real Jardín Botánico
... Nees Institute for Biodiversity of Plants, University of Bonn, Meckenheimer Allee 170, D-53115 Bonn, Germany ...
... Nees Institute for Biodiversity of Plants, University of Bonn, Meckenheimer Allee 170, D-53115 Bonn, Germany ...
PAX6 mRNA Transcript Analysis in Various Ocular/Non
... the ocular/non-ocular tissues expressed the β-actin gene equal levels and showed 540bp amplification. Majority of the genes commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expressi ...
... the ocular/non-ocular tissues expressed the β-actin gene equal levels and showed 540bp amplification. Majority of the genes commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expressi ...
Non-conflict theories for the evolution of genomic imprinting
... reasoned, co-adaptation of these traits, especially those involved in close maternal–offspring interactions, should result and they modelled the evolution of imprinting from this standpoint. By way of example, in their single-locus model (in which offspring fitness was greatest for those expressing ...
... reasoned, co-adaptation of these traits, especially those involved in close maternal–offspring interactions, should result and they modelled the evolution of imprinting from this standpoint. By way of example, in their single-locus model (in which offspring fitness was greatest for those expressing ...
Phenotypic overlap in the contribution of individual genes to CNV
... known phenotypically, but for which detailed phenotypic information for their mouse and/or zebrafish orthologs is available. Here we present an ontology-based approach to identify similarities between human disease manifestations and the mutational phenotypes in characterised model organism genes, a ...
... known phenotypically, but for which detailed phenotypic information for their mouse and/or zebrafish orthologs is available. Here we present an ontology-based approach to identify similarities between human disease manifestations and the mutational phenotypes in characterised model organism genes, a ...
5 Genetic Analysis of Kidney Disease in Mice
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
A pair of Sox: distinct and overlapping functions of
... mutants do not straighten their body axis as wild types and sox9ahi1134 mutants do, giving a curly-down body axis (Fig. 3A,C,E). This phenotype could result from the brief expression of sox9b in the notochord at about 20 hpf. Animals injected with sox9b MOs also show the curly-down phenotype, suppor ...
... mutants do not straighten their body axis as wild types and sox9ahi1134 mutants do, giving a curly-down body axis (Fig. 3A,C,E). This phenotype could result from the brief expression of sox9b in the notochord at about 20 hpf. Animals injected with sox9b MOs also show the curly-down phenotype, suppor ...
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and
... 2006), and the non-teleostean gar and bowfin (Amemiya and Youson 2004). All these hormones share consistent structural features, including a conserved N-glycosylation site and 10 invariant cysteine residues that form intrachain disulfide bonds. Also, an 11th conserved cysteine residue that is critic ...
... 2006), and the non-teleostean gar and bowfin (Amemiya and Youson 2004). All these hormones share consistent structural features, including a conserved N-glycosylation site and 10 invariant cysteine residues that form intrachain disulfide bonds. Also, an 11th conserved cysteine residue that is critic ...
Compound leaves: equal to the sum of their parts?
... (stm) and knotted1 (kn1) in Arabidopsis and maize, respectively, result in plants that are unable to maintain a SAM (Long et al., 1996; Vollbrecht et al., 2000). Maize plants that misexpress KNOX1 genes outside of their normal domain have ectopic proliferation of tissue in leaves, described as knots ...
... (stm) and knotted1 (kn1) in Arabidopsis and maize, respectively, result in plants that are unable to maintain a SAM (Long et al., 1996; Vollbrecht et al., 2000). Maize plants that misexpress KNOX1 genes outside of their normal domain have ectopic proliferation of tissue in leaves, described as knots ...
Molecular Mechanisms of Developmental Review
... lin-14 mRNA, a region with seven copies of a short sequence that could base pair with the lin-4 RNAs (Wightman et al., 1991; Yeh, 1991). In addition, these 3⬘ sequences are sufficient to confer appropriate temporal regulation on the translation of a heterologous gene, implicating these sites as dire ...
... lin-14 mRNA, a region with seven copies of a short sequence that could base pair with the lin-4 RNAs (Wightman et al., 1991; Yeh, 1991). In addition, these 3⬘ sequences are sufficient to confer appropriate temporal regulation on the translation of a heterologous gene, implicating these sites as dire ...
The Evolution of CONSTANS-Like Gene Families
... Copyright © 2003 American Society of Plant Biologists. All rights reserved. ...
... Copyright © 2003 American Society of Plant Biologists. All rights reserved. ...
The somatic-visceral subdivision of the embryonic mesoderm is
... protein corresponds to the 2xPE-sna fusion gene (Fig. 2C). The distribution of sna mRNAs was visualized by in situ hybridization using a digoxigenin-labeled sna antisense RNA probe. As predicted from previous analyses of the PE promoter sequence (Jiang et al., 1991; Jiang and Levine, 1993), the synt ...
... protein corresponds to the 2xPE-sna fusion gene (Fig. 2C). The distribution of sna mRNAs was visualized by in situ hybridization using a digoxigenin-labeled sna antisense RNA probe. As predicted from previous analyses of the PE promoter sequence (Jiang et al., 1991; Jiang and Levine, 1993), the synt ...
Genome-Wide Analysis of In Vivo Binding of the Master Regulator
... to primary metabolism, sugar transport and extracellular polysaccharide hydrolysis, as well as antibiotic production [32]. Moreover, evidence for the functional importance of several of these sequences was demonstrated by showing DasR binding in vitro and changes in the level of associated transcrip ...
... to primary metabolism, sugar transport and extracellular polysaccharide hydrolysis, as well as antibiotic production [32]. Moreover, evidence for the functional importance of several of these sequences was demonstrated by showing DasR binding in vitro and changes in the level of associated transcrip ...
A method for finding molecular signatures from gene expression data
... relevant information (cancer vs. healthy, survival time, etc). Provide insight into biological mechanisms and processes and have potential diagnostic use. However, searching for molecular signatures often done using a very diverse and ad-hoc methodology. ...
... relevant information (cancer vs. healthy, survival time, etc). Provide insight into biological mechanisms and processes and have potential diagnostic use. However, searching for molecular signatures often done using a very diverse and ad-hoc methodology. ...