factor occupancy and gene expression Effects of sequence variation
... DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single factor (Fig. 1C). The result indicates either that these regions of open chromatin were more accessible to TFs before binding, or that the recruitment of many TFs to these regions resulted in more exte ...
... DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single factor (Fig. 1C). The result indicates either that these regions of open chromatin were more accessible to TFs before binding, or that the recruitment of many TFs to these regions resulted in more exte ...
GCF (T-14): sc-133419 - Santa Cruz Biotechnology
... GCF (GC-rich sequence DNA-binding factor), also known as C2orf3 (chromosome 2 open reading frame 3), transcription factor 9 (TCF-9) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of β-Actin, EGFR and calciumdependent pr ...
... GCF (GC-rich sequence DNA-binding factor), also known as C2orf3 (chromosome 2 open reading frame 3), transcription factor 9 (TCF-9) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of β-Actin, EGFR and calciumdependent pr ...
Linköping University Post Print Gene products of chromosome 11q and their
... breast cancers is indeed intriguing. As noted, over-expression of cyclin D1 protein has been linked to lack of tamoxifen response but not to a direct agonist effect. Hypothetically, another gene co-amplified with CCND1 might be responsible for the agonistic effect of tamoxifen. Furthermore, genes ...
... breast cancers is indeed intriguing. As noted, over-expression of cyclin D1 protein has been linked to lack of tamoxifen response but not to a direct agonist effect. Hypothetically, another gene co-amplified with CCND1 might be responsible for the agonistic effect of tamoxifen. Furthermore, genes ...
Functions of the nonsense-mediated RNA decay pathway in Drosophila development. PLoS Genetics 2 , 2143-2154.
... (A) FLP/MARCM system used to generate GFP-labeled homozygous mutant tracheal cell clones in heterozygous animals. Heterozygous cells have mutations (asterisk) in trans to a GAL80 transgene (triangle) that inhibits expression of a tracheal-specific, btl-GAL4-driven GFP transgene on another chromosome ...
... (A) FLP/MARCM system used to generate GFP-labeled homozygous mutant tracheal cell clones in heterozygous animals. Heterozygous cells have mutations (asterisk) in trans to a GAL80 transgene (triangle) that inhibits expression of a tracheal-specific, btl-GAL4-driven GFP transgene on another chromosome ...
Novel regulatio pendage transformation
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
Molecular function - SGD-Wiki - Saccharomyces Genome Database
... products, for example, fructose-bisphosphate aldolase activity or protein serine/threonine kinase activity. Biological process: the broad biological goals, such as mitosis or DNA replication, that are accomplished by ordered assemblies of molecular functions. Cellular component: subcellular structur ...
... products, for example, fructose-bisphosphate aldolase activity or protein serine/threonine kinase activity. Biological process: the broad biological goals, such as mitosis or DNA replication, that are accomplished by ordered assemblies of molecular functions. Cellular component: subcellular structur ...
A tandem repeat in decay accelerating factor 1 induced autoimmunity
... 15], prostaglandin PGE2 [16], and tissue specific factors [17]. Although there is evidence that DAF mRNA stability can be affected by tissue specific factors [17] and inflammation [18], most studies suggest that expression is primarily modulated at transcription [15–17, 19, 20]. The human DAF promot ...
... 15], prostaglandin PGE2 [16], and tissue specific factors [17]. Although there is evidence that DAF mRNA stability can be affected by tissue specific factors [17] and inflammation [18], most studies suggest that expression is primarily modulated at transcription [15–17, 19, 20]. The human DAF promot ...
Genomic Organization of Evolutionarily Correlated Genes in
... of a positive feedback loop connecting periodic genome layout to the cellular conformation of chromosomes on to transcriptional control. 4 Indeed, through the use of a thermodynamic model of chromosome folding where transcription factors (TFs) cross-link distant binding sites, a periodic relative ge ...
... of a positive feedback loop connecting periodic genome layout to the cellular conformation of chromosomes on to transcriptional control. 4 Indeed, through the use of a thermodynamic model of chromosome folding where transcription factors (TFs) cross-link distant binding sites, a periodic relative ge ...
Touring Ensembl: A practical guide to genome browsing Open Access
... [12-14]. Within only 200 bp upstream of the translational start site, binding sites for proteins such as NF-κB, AP-1, and NFAT (nuclear factor of activated T-cells), DNase I hypersensitive sites and a TATA box can all be found. These regions have been shown to be involved in the control of T-cell me ...
... [12-14]. Within only 200 bp upstream of the translational start site, binding sites for proteins such as NF-κB, AP-1, and NFAT (nuclear factor of activated T-cells), DNase I hypersensitive sites and a TATA box can all be found. These regions have been shown to be involved in the control of T-cell me ...
The Biology and Evolution of Mammalian Y Chromosomes
... X and Y chromosomes’ shared evolutionary origins, as an ordinary pair of autosomes. “Ampliconic” regions of the MSY are composed of sequences that exhibit striking similarity – as much as 99.99% identity over tens or hundreds of kilobases (kb) – to other MSY sequences. Some ampliconic sequences are ...
... X and Y chromosomes’ shared evolutionary origins, as an ordinary pair of autosomes. “Ampliconic” regions of the MSY are composed of sequences that exhibit striking similarity – as much as 99.99% identity over tens or hundreds of kilobases (kb) – to other MSY sequences. Some ampliconic sequences are ...
Construction and Characterization of a Highly Regulable Expression
... conditions that induce protein production. Unfortunately, however, numerous researchers have found that these vectors produce significant amounts of protein even when grown under repressed conditions. We describe here a new expression vector, pLAC11, which was designed to be more regulable and thus ...
... conditions that induce protein production. Unfortunately, however, numerous researchers have found that these vectors produce significant amounts of protein even when grown under repressed conditions. We describe here a new expression vector, pLAC11, which was designed to be more regulable and thus ...
Wnt8 Is Required for Growth-Zone Establishment and Development
... spider development. In Achaearanea, At-Krüppel-2 (At-Kr2) is expressed in a stripe marking the presumptive posterior and anterior regions of L3 and L4, respectively (Figure S3F). In At-Wnt8pRNAi embryos, this expression domain of At-Kr2 is greatly expanded, confirming our observation that L3/L4 is ...
... spider development. In Achaearanea, At-Krüppel-2 (At-Kr2) is expressed in a stripe marking the presumptive posterior and anterior regions of L3 and L4, respectively (Figure S3F). In At-Wnt8pRNAi embryos, this expression domain of At-Kr2 is greatly expanded, confirming our observation that L3/L4 is ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... DNA methylation does not affect MEA imprinting. DNA methylation targets a small region upstream of FIS2 distinct from the target of DNA methylation associated with FWA. We show that FWA and FIS2 imprinting requires the maintenance of DNA methylation throughout the plant life cycle, including male ga ...
... DNA methylation does not affect MEA imprinting. DNA methylation targets a small region upstream of FIS2 distinct from the target of DNA methylation associated with FWA. We show that FWA and FIS2 imprinting requires the maintenance of DNA methylation throughout the plant life cycle, including male ga ...
Coordination of replication and transcription along a Drosophila
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
Chpt12_RNAProcessing.doc
... One application currently being explored is the use of designed hammerheads to cleave a particular mRNA, thereby turning off expression of a particular gene. If over-expression or ectopic expression of a defined gene were the cause of some pathology (e.g. some form of cancer), then reducing its expr ...
... One application currently being explored is the use of designed hammerheads to cleave a particular mRNA, thereby turning off expression of a particular gene. If over-expression or ectopic expression of a defined gene were the cause of some pathology (e.g. some form of cancer), then reducing its expr ...
Genetic analysis of genes on the 4th chromosome of
... and low-copy repetitive DNA, often transposons, interspersed with unique DNA (Locke et al., 1999b; Miklos et al., 1988). Chromosome 4 exhibits a further similarity to β-heterochromatin in that the chromosomal protein HP1, thought to be an important constituent of heterochromatin, binds to several si ...
... and low-copy repetitive DNA, often transposons, interspersed with unique DNA (Locke et al., 1999b; Miklos et al., 1988). Chromosome 4 exhibits a further similarity to β-heterochromatin in that the chromosomal protein HP1, thought to be an important constituent of heterochromatin, binds to several si ...
Genome-wide identification and analysis of the SGR
... CmSGR ORF lengths ranged from 504 (CmSGR4) to 774 bp (CmSGR2), molecular weights ranged from 19.02 (CmSGR4) to 29.48 kDa (CmSGR2), and pI values ranged from 6.19 (CmSGR4) to 8.72 (CmSGR2) (Table 1). CmSGR genes were distributed on all chromosome of the melon genome. CmSGR1 and CmSGR2 genes were loca ...
... CmSGR ORF lengths ranged from 504 (CmSGR4) to 774 bp (CmSGR2), molecular weights ranged from 19.02 (CmSGR4) to 29.48 kDa (CmSGR2), and pI values ranged from 6.19 (CmSGR4) to 8.72 (CmSGR2) (Table 1). CmSGR genes were distributed on all chromosome of the melon genome. CmSGR1 and CmSGR2 genes were loca ...
Epigenetic mechanisms regulate placental c-myc
... Helix-Loop-Helix Leucine Zipper) domain and can bind to Enhancer Box sequences (E-boxes) via its bHLH domain recruiting histone acetyltransferases and further, dimerizing with its partner Max, with its leucine zipper domain, thus regulating the expression of a large number of downstream target genes ...
... Helix-Loop-Helix Leucine Zipper) domain and can bind to Enhancer Box sequences (E-boxes) via its bHLH domain recruiting histone acetyltransferases and further, dimerizing with its partner Max, with its leucine zipper domain, thus regulating the expression of a large number of downstream target genes ...
lac
... doesn’t waste time and energy making mRNA and proteins that are not needed. The lac genes are only transcribed if lactose is present in the growth medium. ...
... doesn’t waste time and energy making mRNA and proteins that are not needed. The lac genes are only transcribed if lactose is present in the growth medium. ...
Arabidopsis transcriptional regulation by light stress via hydrogen
... by the Arabidopsis genome-sequencing project. Therefore, we decided to clone the promoter region of this gene. The promoter region of ELIP2, 21907 to 22 bp relative to the translation start site, was fused to the firefly luciferase reporter gene (Millar et al. 1992) and introduced into Arabidopsis p ...
... by the Arabidopsis genome-sequencing project. Therefore, we decided to clone the promoter region of this gene. The promoter region of ELIP2, 21907 to 22 bp relative to the translation start site, was fused to the firefly luciferase reporter gene (Millar et al. 1992) and introduced into Arabidopsis p ...
The B-cell maturation factor Blimp-1 specifies vertebrate slow
... and shortening of the body axis (data not shown), consistent with a previously described role of Blimp-1 in regulating vertebrate gastrulation15. Examination of the slow muscle lineage in these morphants at early stages of somitogenesis showed that its development was completely impaired, as indicat ...
... and shortening of the body axis (data not shown), consistent with a previously described role of Blimp-1 in regulating vertebrate gastrulation15. Examination of the slow muscle lineage in these morphants at early stages of somitogenesis showed that its development was completely impaired, as indicat ...
Evolutionary relationships of the Tas2r receptor gene families in
... (ftp://ftp.ncbi.nlm.nih.gov/blast). HSPs found by the same query sequence, and lying in proximity to each other on a single chromosome, were assembled into complete genes using the publicly available software GeneWise from the Wise2 package by Ewan Birney at the Sanger Centre, Hinxton, UK (http://ww ...
... (ftp://ftp.ncbi.nlm.nih.gov/blast). HSPs found by the same query sequence, and lying in proximity to each other on a single chromosome, were assembled into complete genes using the publicly available software GeneWise from the Wise2 package by Ewan Birney at the Sanger Centre, Hinxton, UK (http://ww ...