CHAPTER 1 LITERATURE SURVEY
... transcribed from plasmids) containing the viral genome directly into cells, as was first demonstrated with Poliovirus (PV; Racaniello & Baltimore 1981). Due to their generally smaller genome sizes compared to DNA viruses, whole RNA virus genomes can be cloned as cDNA and manipulated at will. This ap ...
... transcribed from plasmids) containing the viral genome directly into cells, as was first demonstrated with Poliovirus (PV; Racaniello & Baltimore 1981). Due to their generally smaller genome sizes compared to DNA viruses, whole RNA virus genomes can be cloned as cDNA and manipulated at will. This ap ...
1 Depleting gene activities in early Drosophila embryos
... phenotype in Figure 2). These embryos showed variable germ band retraction and head defect phenotypes reminiscent of weak dpp alleles, (Spencer et al., 1982; Irish and Gelbart, 1987). In addition, a number of shRNAs targeting other genes also led to F1 embryonic lethality and in some cases cuticle ...
... phenotype in Figure 2). These embryos showed variable germ band retraction and head defect phenotypes reminiscent of weak dpp alleles, (Spencer et al., 1982; Irish and Gelbart, 1987). In addition, a number of shRNAs targeting other genes also led to F1 embryonic lethality and in some cases cuticle ...
Chapter 14
... • The early genes transcribed by host RNA polymerase following infection include, or comprise, regulators required for expression of the middle set of phage genes. • The middle group of genes includes regulators to transcribe the late genes. • This results in the ordered expression of groups of gene ...
... • The early genes transcribed by host RNA polymerase following infection include, or comprise, regulators required for expression of the middle set of phage genes. • The middle group of genes includes regulators to transcribe the late genes. • This results in the ordered expression of groups of gene ...
Pairing of homologous regions in the mouse genome is associated
... requires several interleaved layers of control. Apart from protein factor binding to cis regulatory regions, modifications of DNA and chromatin, position of the gene in nuclear space, and an intricate network of chromosome associations in trans determine the expression state of a particular gene. Of ...
... requires several interleaved layers of control. Apart from protein factor binding to cis regulatory regions, modifications of DNA and chromatin, position of the gene in nuclear space, and an intricate network of chromosome associations in trans determine the expression state of a particular gene. Of ...
Analysis of TALE superclass homeobox genes
... atypical homeodomain that had not been properly identified due to four separate frameshifts and other errors of the EST sequence within the homeobox. This gene was named ceh-25 and searches of the databases revealed several mammalian ESTs with high similarity, which were grouped together under a new ...
... atypical homeodomain that had not been properly identified due to four separate frameshifts and other errors of the EST sequence within the homeobox. This gene was named ceh-25 and searches of the databases revealed several mammalian ESTs with high similarity, which were grouped together under a new ...
Package `acde` - USTC Open Source Software Mirror
... Description This package provides a multivariate inferential analysis method for detecting differentially expressed genes in gene expression data. It uses artificial components, close to the data's principal components but with an exact interpretation in terms of differential genetic expression, to ...
... Description This package provides a multivariate inferential analysis method for detecting differentially expressed genes in gene expression data. It uses artificial components, close to the data's principal components but with an exact interpretation in terms of differential genetic expression, to ...
Life: The Science of Biology, 8e
... Fertility factors (F factors) have genes needed for conjugation; F factor can be transferred during conjugation. ...
... Fertility factors (F factors) have genes needed for conjugation; F factor can be transferred during conjugation. ...
PDF
... The analysis of bilaterally expressed markers, the homeobox gene ceh-36 and the FMRFamide gene flp-6, displayed an unexpected phenotype. Expression of the bilateral markers was unaffected, lost in either ASEL or ASER, or lost in both (Fig. 2A, Table 1). This observation raised the possibility that t ...
... The analysis of bilaterally expressed markers, the homeobox gene ceh-36 and the FMRFamide gene flp-6, displayed an unexpected phenotype. Expression of the bilateral markers was unaffected, lost in either ASEL or ASER, or lost in both (Fig. 2A, Table 1). This observation raised the possibility that t ...
Plant–nematode interactions
... Genome-wide approaches with plant-parasitic nematodes Broad investigations of the genomes and gene products of plant-parasitic nematodes are also underway. More than 200 000 nematode ESTs from 28 nematode species, excluding C. elegans but including 19 animal- and seven plant-parasitic nematode speci ...
... Genome-wide approaches with plant-parasitic nematodes Broad investigations of the genomes and gene products of plant-parasitic nematodes are also underway. More than 200 000 nematode ESTs from 28 nematode species, excluding C. elegans but including 19 animal- and seven plant-parasitic nematode speci ...
Transcriptional Regulation by FOXP1, FOXP2, and
... Previous studies have already demonstrated that FOXP1, FOXP2, and FOXP4 proteins homodimerize with themselves and heterodimerize with each other in transfected HEK293 cells (Li et al. 2004). We performed coimmunoprecipitation assays to confirm that FOXP1, FOXP2, and FOXP4 heterodimerize in the HEK29 ...
... Previous studies have already demonstrated that FOXP1, FOXP2, and FOXP4 proteins homodimerize with themselves and heterodimerize with each other in transfected HEK293 cells (Li et al. 2004). We performed coimmunoprecipitation assays to confirm that FOXP1, FOXP2, and FOXP4 heterodimerize in the HEK29 ...
Transcriptional control of glial cell development in Drosophila
... process is laborious, surprisingly few cis-regulatory DNAs have been examined in any detail (Davidson, 2001; Markstein and Levine, 2002). However, common features of animal regulatory sequences have become apparent (Arnone and Davidson, 1997; Howard and Davidson, 2004; Levine and Tjian, 2003). Most ...
... process is laborious, surprisingly few cis-regulatory DNAs have been examined in any detail (Davidson, 2001; Markstein and Levine, 2002). However, common features of animal regulatory sequences have become apparent (Arnone and Davidson, 1997; Howard and Davidson, 2004; Levine and Tjian, 2003). Most ...
mRNA Export - e
... •The general RanGTP-dependent protein export receptor CRM1 can be involved in the nuclear export of a subset of transcripts, such as mRNAs of several protooncogenes and cytokines, that contain AU-rich elements. •CRM1 itself does not bind to RNA, instead recruiting NEScontaining adaptor proteins that ...
... •The general RanGTP-dependent protein export receptor CRM1 can be involved in the nuclear export of a subset of transcripts, such as mRNAs of several protooncogenes and cytokines, that contain AU-rich elements. •CRM1 itself does not bind to RNA, instead recruiting NEScontaining adaptor proteins that ...
RNA and Protein Synthesis
... In prokaryotes, RNA synthesis and protein synthesis take place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s nucleus and then moves to the cytoplasm to play a role in the production of protein. Our focus here is on transcription in eukaryotic cells. Transcription requires an enzyme, ...
... In prokaryotes, RNA synthesis and protein synthesis take place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s nucleus and then moves to the cytoplasm to play a role in the production of protein. Our focus here is on transcription in eukaryotic cells. Transcription requires an enzyme, ...
publication
... area known to be gene-rich and prone to chromosomal breakage. Aberrations at 7q22 are commonly observed in myeloid leukemias and myelodysplastic syndromes, with critical deleted regions being mapped using fluorescent in situ hybridization (1–4) and polymorphic microsatellite markers (5). Specificall ...
... area known to be gene-rich and prone to chromosomal breakage. Aberrations at 7q22 are commonly observed in myeloid leukemias and myelodysplastic syndromes, with critical deleted regions being mapped using fluorescent in situ hybridization (1–4) and polymorphic microsatellite markers (5). Specificall ...
Molecular Design of Expression Systems
... upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regulatory mutant) or the composition (inducer or corepressor concentration) of the growth medium in order to switch from low to high promoter activity. Limitations o ...
... upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regulatory mutant) or the composition (inducer or corepressor concentration) of the growth medium in order to switch from low to high promoter activity. Limitations o ...
homolog of the agouti gene
... mouse cDNA clone was hybridized to Southern blots containing human genomic DNA. Reduced stringency hybridization conditions allowed for detection of cross-hybridizing DNA fragments with a variety of restriction enzymes (data not shown). Based on this result, the same mouse agouti cDNA probe was used ...
... mouse cDNA clone was hybridized to Southern blots containing human genomic DNA. Reduced stringency hybridization conditions allowed for detection of cross-hybridizing DNA fragments with a variety of restriction enzymes (data not shown). Based on this result, the same mouse agouti cDNA probe was used ...
Regulatory Genes Controlling MPG7 Expression
... screened for growth on nitrate minimal medium. DNA gel blot analysis of two C160 and four (21105 hygromycin-resistanttransformants showed integration of pNIT2 DNA (Figure 4A, lanes 4 and 5, and lanes 7 to 10, respectively). However, these transformants failed to grow on nitrate (Figure 46) and were ...
... screened for growth on nitrate minimal medium. DNA gel blot analysis of two C160 and four (21105 hygromycin-resistanttransformants showed integration of pNIT2 DNA (Figure 4A, lanes 4 and 5, and lanes 7 to 10, respectively). However, these transformants failed to grow on nitrate (Figure 46) and were ...
Altered cellular proliferation and mesoderm
... expand in vitro of several cell types including lymphocytes and fibroblasts. In addition, M33 null mutant mice show an aggravation of the skeletal malformations when treated to RA at embryonic day 7.5, leading to the hypothesis that, during development, the M33 gene might play a role in defining acc ...
... expand in vitro of several cell types including lymphocytes and fibroblasts. In addition, M33 null mutant mice show an aggravation of the skeletal malformations when treated to RA at embryonic day 7.5, leading to the hypothesis that, during development, the M33 gene might play a role in defining acc ...
Fig 16.12a - McGraw Hill Higher Education
... Activators are transcription factors that bind to enhancers Activators are responsible for much of the variation in levels of transcription of different genes Increase levels of transcription by interacting directly or indirectly with basal factors at the promoter • 3-dimensional complex of protein ...
... Activators are transcription factors that bind to enhancers Activators are responsible for much of the variation in levels of transcription of different genes Increase levels of transcription by interacting directly or indirectly with basal factors at the promoter • 3-dimensional complex of protein ...
Whole-transcriptome RNAseq analysis from minute amount of total
... increased the transcript coverage, which has in turn enhanced our ability to detect novel rare transcripts, novel alternative splice isoforms and direct measurement of transcript abundance (3). These technologies are greatly accelerating our understanding of the complexity of gene expression, regula ...
... increased the transcript coverage, which has in turn enhanced our ability to detect novel rare transcripts, novel alternative splice isoforms and direct measurement of transcript abundance (3). These technologies are greatly accelerating our understanding of the complexity of gene expression, regula ...
Genes@ Work: an efficient algorithm for pattern discovery and
... Statistical significance of patterns We are interested in discovering patterns that differentiate the phenotype set from the control set, and that are unlikely to occur by chance. In order to achieve such discrimination of patterns, we define a null hypothesis under which we can compute the probabil ...
... Statistical significance of patterns We are interested in discovering patterns that differentiate the phenotype set from the control set, and that are unlikely to occur by chance. In order to achieve such discrimination of patterns, we define a null hypothesis under which we can compute the probabil ...
Interactions of Mitochondrial and Nuclear Genes
... lines. Unfortunately, with a few exceptions, the comparative genomics strategy has not been successful. Differences between two cytoplasms often may reflect evolutionary divergence and have no correlation with CMS. What is needed, therefore, is to compare recently diverged CMS and fertile lines. Ide ...
... lines. Unfortunately, with a few exceptions, the comparative genomics strategy has not been successful. Differences between two cytoplasms often may reflect evolutionary divergence and have no correlation with CMS. What is needed, therefore, is to compare recently diverged CMS and fertile lines. Ide ...