NanoString™: User Guide | nCounter® Expression Data Analysis
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
TCF11/Nrf1 overexpression increases the intracellular
... the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transfected using calcium phosphate and including CAT as intern ...
... the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transfected using calcium phosphate and including CAT as intern ...
Protocadherin-1: epithelial barrier dysfunction in asthma and eczema Grissel Faura Tellez
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
Hox patterning of the vertebrate axial skeleton
... The axial skeleton in all vertebrates is composed of similar components that extend from anterior to posterior along the body axis: the occipital skull bones and cervical, thoracic, lumbar, sacral, and caudal vertebrae. Despite significant changes in the number and size of these elements during evolu ...
... The axial skeleton in all vertebrates is composed of similar components that extend from anterior to posterior along the body axis: the occipital skull bones and cervical, thoracic, lumbar, sacral, and caudal vertebrae. Despite significant changes in the number and size of these elements during evolu ...
reproductive cell fate transition in plants - Development
... Histone variants are dynamically exchanged in differentiating MMCs Linker histones (H1) are essential modulators of chromatin compaction through binding to the linker DNA and the core nucleosome, thereby stabilizing higher-order chromatin structure (Robinson and Rhodes, 2006). The Arabidopsis genome ...
... Histone variants are dynamically exchanged in differentiating MMCs Linker histones (H1) are essential modulators of chromatin compaction through binding to the linker DNA and the core nucleosome, thereby stabilizing higher-order chromatin structure (Robinson and Rhodes, 2006). The Arabidopsis genome ...
hag expression in Bacillus subtilis is both negatively
... (Caldwell et al., 2001), which are mainly transcribed by the sD-dependent RNA polymerase (Ordal et al., 1993). This conclusion has been derived from the transcription profiling of scoC mutant cells (scoC4), which showed that most of the motility genes, including hag, are transcribed at lower levels ...
... (Caldwell et al., 2001), which are mainly transcribed by the sD-dependent RNA polymerase (Ordal et al., 1993). This conclusion has been derived from the transcription profiling of scoC mutant cells (scoC4), which showed that most of the motility genes, including hag, are transcribed at lower levels ...
The enhancement of ribosomal transcription by the recycling of RNA
... the downstream pre-rRNA promoter would be higher than in the free pool. This hypothetical mechanism of transcription enhancement was suggested some years ago (1) and has been called "Readthrough Enhancement" (5). Our present model of the ribosomal spacer has the Spacer Promoters involved in a stage ...
... the downstream pre-rRNA promoter would be higher than in the free pool. This hypothetical mechanism of transcription enhancement was suggested some years ago (1) and has been called "Readthrough Enhancement" (5). Our present model of the ribosomal spacer has the Spacer Promoters involved in a stage ...
reproductive cell fate transition in plants - Development
... KEY WORDS: Arabidopsis, Plant reproduction, Megaspore mother cell, Heterochromatin, Chromatin modifications, Histone variants ...
... KEY WORDS: Arabidopsis, Plant reproduction, Megaspore mother cell, Heterochromatin, Chromatin modifications, Histone variants ...
Identification of Genes Needed for Regeneration, Stem Cell
... of the phenotype can relate to multiple categories. Table 2 also includes the homology of these genes (if any) and a code that describes the RNAi phenotype in detail (see Figures 1E and 1F as guides for phenotype terminology). RNAi-induced phenotypes and sequence homologies for some genes that are l ...
... of the phenotype can relate to multiple categories. Table 2 also includes the homology of these genes (if any) and a code that describes the RNAi phenotype in detail (see Figures 1E and 1F as guides for phenotype terminology). RNAi-induced phenotypes and sequence homologies for some genes that are l ...
Hox patterning of the vertebrate rib cage
... AP regions of the axial skeleton. These combined results suggest that there are alternative mechanisms by which Hox genes govern patterning of the thoracic region. Part of the difficulty in understanding patterning of the rib cage results from the nature of development of the thoracic skeleton. The ...
... AP regions of the axial skeleton. These combined results suggest that there are alternative mechanisms by which Hox genes govern patterning of the thoracic region. Part of the difficulty in understanding patterning of the rib cage results from the nature of development of the thoracic skeleton. The ...
Expression of floricaula in single cell layers of
... (Fig. 2). This gave a 9.0 kb band for flo-613 and a 5.5 kb band for wild-type plants that lack the 3.5 kb Tam3 element (Fig. 2A, left two lanes). Most of the chimeras had a 9.0 kb band, showing that they carried Tam3 in the flo gene. In addition, all chimeras had a 5.5 kb band of varying intensity, ...
... (Fig. 2). This gave a 9.0 kb band for flo-613 and a 5.5 kb band for wild-type plants that lack the 3.5 kb Tam3 element (Fig. 2A, left two lanes). Most of the chimeras had a 9.0 kb band, showing that they carried Tam3 in the flo gene. In addition, all chimeras had a 5.5 kb band of varying intensity, ...
Linköping University Post Print Neuronal Subtype Specification within a Feed-Forward Loops
... regulation of Col from Ap2/3 and Ap4/FMRFa controlled? Finally, the Col feed-forward loop specifies Ap1/Nplp1 fate, and the absence of Col specifies the Ap2/3 fate, but how is the Ap4/FMRFa fate specified (Figure 1C)? Here we find that the Ap cluster neurons are the last four cells to be born, in t ...
... regulation of Col from Ap2/3 and Ap4/FMRFa controlled? Finally, the Col feed-forward loop specifies Ap1/Nplp1 fate, and the absence of Col specifies the Ap2/3 fate, but how is the Ap4/FMRFa fate specified (Figure 1C)? Here we find that the Ap cluster neurons are the last four cells to be born, in t ...
Hox patterning of the vertebrate rib cage
... AP regions of the axial skeleton. These combined results suggest that there are alternative mechanisms by which Hox genes govern patterning of the thoracic region. Part of the difficulty in understanding patterning of the rib cage results from the nature of development of the thoracic skeleton. The ...
... AP regions of the axial skeleton. These combined results suggest that there are alternative mechanisms by which Hox genes govern patterning of the thoracic region. Part of the difficulty in understanding patterning of the rib cage results from the nature of development of the thoracic skeleton. The ...
DNA methylation profiling identifies epigenetic dysregulation in
... from the control samples when comparing complete methylation profiles. For this, an unsupervised hierarchical clustering was performed which placed the diabetic islet samples as one self-contained group distinct from the control samples in the resulting dendrogram (Supplementary Figure S2). This outc ...
... from the control samples when comparing complete methylation profiles. For this, an unsupervised hierarchical clustering was performed which placed the diabetic islet samples as one self-contained group distinct from the control samples in the resulting dendrogram (Supplementary Figure S2). This outc ...
Molecular Mechanisms of Floral Boundary Formation in Arabidopsis
... in the floral organ primordia and boundaries, and the double mutant combinations of the three CUC genes form fusions between adjacent floral organs [5,6,61]. More importantly, CUC genes are key nodes of the genetic network that regulates floral organ boundaries. They act to control a suite of bounda ...
... in the floral organ primordia and boundaries, and the double mutant combinations of the three CUC genes form fusions between adjacent floral organs [5,6,61]. More importantly, CUC genes are key nodes of the genetic network that regulates floral organ boundaries. They act to control a suite of bounda ...
DNA cytosine methylation in plant development
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
Tumor metastasis-associated human MTA1 gene and its MTA1
... we cloned the human MTA1 gene and found it to be over-expressed in a variety of human cell lines (breast, ovarian, lung, gastric and colorectal cancer but not melanoma or sarcoma) and cancerous tissues (breast, esophageal, colorectal, gastric and pancreatic cancer). We found a close similarity betwe ...
... we cloned the human MTA1 gene and found it to be over-expressed in a variety of human cell lines (breast, ovarian, lung, gastric and colorectal cancer but not melanoma or sarcoma) and cancerous tissues (breast, esophageal, colorectal, gastric and pancreatic cancer). We found a close similarity betwe ...
RT2 Profiler™ PCR Arrays: Pathway
... to convert RNA into first strand cDNA, but also for the removal of genomic DNA from the RNA in the same simple two-step 30-minute reaction. A proprietary genomic DNA elimination buffer completely removes any residual genomic DNA from your RNA sample. Then, the optimized formulation also allows you to ...
... to convert RNA into first strand cDNA, but also for the removal of genomic DNA from the RNA in the same simple two-step 30-minute reaction. A proprietary genomic DNA elimination buffer completely removes any residual genomic DNA from your RNA sample. Then, the optimized formulation also allows you to ...
New functions of the Drosophila rhomboid gene
... driving the localized expression of an active gene of interest in place of the passive reporter gene. Thus, mobilization of an enhancer trap-like vector carrying a cDNA of interest should result in transposon insertions near endogenous genomic enhancers which could drive localized expression of the ...
... driving the localized expression of an active gene of interest in place of the passive reporter gene. Thus, mobilization of an enhancer trap-like vector carrying a cDNA of interest should result in transposon insertions near endogenous genomic enhancers which could drive localized expression of the ...
Structure, expression and chromosomal location of the Oct
... onset of zygotic Oct-4 expression does not occur until compaction begins at 8-ceils, suggesting that there might be other regulatory factors responsible for initiating Oct-4 expression. ...
... onset of zygotic Oct-4 expression does not occur until compaction begins at 8-ceils, suggesting that there might be other regulatory factors responsible for initiating Oct-4 expression. ...
WEREWOLF, a Regulator of Root Hair Pattern
... A key floral activator, FT, integrates stimuli from long-day, vernalization, and autonomous pathways and triggers flowering by directly regulating floral meristem identity genes in Arabidopsis (Arabidopsis thaliana). Since a small amount of FT transcript is sufficient for flowering, the FT level is ...
... A key floral activator, FT, integrates stimuli from long-day, vernalization, and autonomous pathways and triggers flowering by directly regulating floral meristem identity genes in Arabidopsis (Arabidopsis thaliana). Since a small amount of FT transcript is sufficient for flowering, the FT level is ...
Misregulation of pre-mRNA splicing that causes human diseases
... About one third of all human genes are subject to alternative splicing. The molecular mechanisms that regulate alternative splice site usage are beginning to emerge and show that transcription and pre-mRNA processing are integrated processes that can be modified by cellular signals. Several diseases ...
... About one third of all human genes are subject to alternative splicing. The molecular mechanisms that regulate alternative splice site usage are beginning to emerge and show that transcription and pre-mRNA processing are integrated processes that can be modified by cellular signals. Several diseases ...
SPT3 interacts with TFIID to allow normal transcription in
... have been shown to cause pleiotropic phenotypes and to lead to changes in transcription in vivo. Here, we report the cloning and analysis of one such mutation, sptl5-21, which causes a single-amino-acid substitution in a conserved residue of TFIID. Surprisingly, the sptl5-21 mutation does not affect ...
... have been shown to cause pleiotropic phenotypes and to lead to changes in transcription in vivo. Here, we report the cloning and analysis of one such mutation, sptl5-21, which causes a single-amino-acid substitution in a conserved residue of TFIID. Surprisingly, the sptl5-21 mutation does not affect ...
Regulation of Na/K-ATPase β1 -subunit gene
... including that of Na/K-ATPase α3 subunit, in cultured neonatal rat cardiac myocytes. The aim of this work was to determine whether ouabain and other hypertrophic stimuli affect Na/K-ATPase β1 subunit gene expression. When myocytes were exposed to non-toxic concentrations of ouabain, ouabain increase ...
... including that of Na/K-ATPase α3 subunit, in cultured neonatal rat cardiac myocytes. The aim of this work was to determine whether ouabain and other hypertrophic stimuli affect Na/K-ATPase β1 subunit gene expression. When myocytes were exposed to non-toxic concentrations of ouabain, ouabain increase ...