MiRNA_GO_Meeting_August2015
... When annotating a miRNA we should minimally aim to capture the following: 1. The miRNA’s main role in gene silencing and its target(s); gene silencing by miRNA negative regulation of gene expression mRNA binding involved in post-transcriptional gene silencing by miRNA target mRNA indicated in the an ...
... When annotating a miRNA we should minimally aim to capture the following: 1. The miRNA’s main role in gene silencing and its target(s); gene silencing by miRNA negative regulation of gene expression mRNA binding involved in post-transcriptional gene silencing by miRNA target mRNA indicated in the an ...
The Three Amnesias - University of Florida College of Public Health
... to as the “medial limbic circuit”, is completed by thalamic projections to the cingulate gyrus and cingulate projections, via the cingulate bundle or cingulum, which extend back to the hippocampus. The hippocampus also receives subcortical projections from the basal forebrain (medial septal nucleus ...
... to as the “medial limbic circuit”, is completed by thalamic projections to the cingulate gyrus and cingulate projections, via the cingulate bundle or cingulum, which extend back to the hippocampus. The hippocampus also receives subcortical projections from the basal forebrain (medial septal nucleus ...
Three Cases of Enduring Memory Impairment after Bilateral Damage
... years or more can occur after damage limited to the hippocampal formation. Findings from studies with experimental animals are consistent with the findings from amnesic patients. The present results substantiate the idea that severity of memory impairment is dependent on locus and extent of damage w ...
... years or more can occur after damage limited to the hippocampal formation. Findings from studies with experimental animals are consistent with the findings from amnesic patients. The present results substantiate the idea that severity of memory impairment is dependent on locus and extent of damage w ...
Rapid divergence and diversification of mammalian duplicate gene
... While small Ne is also thought to result in a higher prevalence of subfunctionalization [34], this process does not appear to play a major role in the retention of duplicate genes in either lineage. One possible reason for this observation is that subfunctionalization may be more common in duplicate ...
... While small Ne is also thought to result in a higher prevalence of subfunctionalization [34], this process does not appear to play a major role in the retention of duplicate genes in either lineage. One possible reason for this observation is that subfunctionalization may be more common in duplicate ...
Epigenetic Control of Apomixis
... regions of chromosomes and the repetitive elements are heavily methylated. The level of 5-mC is determined by DNA methylation and demethylation processes. Methylation can be removed from the DNA by active and/or passive mechanisms. Promiscuous methylation requires to be pruned by demethylases to cre ...
... regions of chromosomes and the repetitive elements are heavily methylated. The level of 5-mC is determined by DNA methylation and demethylation processes. Methylation can be removed from the DNA by active and/or passive mechanisms. Promiscuous methylation requires to be pruned by demethylases to cre ...
pdf
... 1. The natural inducer (or antirepressor), is allolactose, an analog of lactose. It is made as a metabolic by-product of the reaction catalyzed by β-galactosidase. Usually this enzyme catalyzes the cleavage of lactose to galactose + glucose, but occasionally it will catalyze an isomerization to form ...
... 1. The natural inducer (or antirepressor), is allolactose, an analog of lactose. It is made as a metabolic by-product of the reaction catalyzed by β-galactosidase. Usually this enzyme catalyzes the cleavage of lactose to galactose + glucose, but occasionally it will catalyze an isomerization to form ...
The eyeless homeodomain is dispensable for eye development in
... It has been shown previously that full-length ey cDNA was able to efficiently rescue the eye phenotype of ey2 mutants when expressed in the eye disc under the control of the eye-specific ey enhancer (Halder et al. 1998). To assess the contribution of the EY DNA-binding domains to eye development, we ...
... It has been shown previously that full-length ey cDNA was able to efficiently rescue the eye phenotype of ey2 mutants when expressed in the eye disc under the control of the eye-specific ey enhancer (Halder et al. 1998). To assess the contribution of the EY DNA-binding domains to eye development, we ...
Gene Section HMGA2 (high mobility group AT-hook 2) in Oncology and Haematology
... In a majority of cases, there is no fusion gene: the breakpoint is located 10 kb up to 100 kb 5' to HMGIC; the recombinational repair gene RAD51B is a candidate to be the partner gene of HMGIC in t(12;14). In two cases (out of 81 primary tumors) exon 7 of RAD51B was fused in frame to either exon 2 o ...
... In a majority of cases, there is no fusion gene: the breakpoint is located 10 kb up to 100 kb 5' to HMGIC; the recombinational repair gene RAD51B is a candidate to be the partner gene of HMGIC in t(12;14). In two cases (out of 81 primary tumors) exon 7 of RAD51B was fused in frame to either exon 2 o ...
This article appeared in a journal published by Elsevier. The
... that this differential virulence is mediated by the 2b gene [21]. Alignment of aa sequences of 2b proteins from 39 CMV strains revealed that 2b proteins of subgroup I (A plus B) strains are typically longer (110–111 aa) than those of subgroup II (100 aa) [5,22] (Supplementary Fig. S1). These two siz ...
... that this differential virulence is mediated by the 2b gene [21]. Alignment of aa sequences of 2b proteins from 39 CMV strains revealed that 2b proteins of subgroup I (A plus B) strains are typically longer (110–111 aa) than those of subgroup II (100 aa) [5,22] (Supplementary Fig. S1). These two siz ...
Identification of Mga1, a G‐protein α‐subunit gene involved in
... clusters involved in the biosynthesis of secondary metabolites of Monascus spp., such as citrinin and monacolin K, have been identified (Shimizu et al., 2007; Chen et al., 2008b). Based on the genetic information, a genetic modification method has also been proposed (Fu et al., 2007; Jia et al., 201 ...
... clusters involved in the biosynthesis of secondary metabolites of Monascus spp., such as citrinin and monacolin K, have been identified (Shimizu et al., 2007; Chen et al., 2008b). Based on the genetic information, a genetic modification method has also been proposed (Fu et al., 2007; Jia et al., 201 ...
The Effect of Chromosomal Position on the Expression of the
... Defective P elements containing either an 8.2 kb Sal I fragment or a 7.2 kb Hind Ill fragment of chromosomal DNA that includes the rosy gene were constructed and injected into embryos from a rosy mutant strain (ry”) as described in Experimental Procedures. To be useful for studies of position effect ...
... Defective P elements containing either an 8.2 kb Sal I fragment or a 7.2 kb Hind Ill fragment of chromosomal DNA that includes the rosy gene were constructed and injected into embryos from a rosy mutant strain (ry”) as described in Experimental Procedures. To be useful for studies of position effect ...
The global repressor FliZ antagonizes gene
... In order to test whether FliZ is able to bind to DNA in vitro, the E. coli protein was purified and subject to EMSA with DNA fragments containing the promoters of mlrA and yciR. The mlrA gene encodes a MerR-like regulator essential for transcriptional activation of the central curli regulator CsgD (S ...
... In order to test whether FliZ is able to bind to DNA in vitro, the E. coli protein was purified and subject to EMSA with DNA fragments containing the promoters of mlrA and yciR. The mlrA gene encodes a MerR-like regulator essential for transcriptional activation of the central curli regulator CsgD (S ...
embr201439791-sup-0014
... Table S3. Mapped sequences were run through featureCount and differential expression analysis was performed on the raw counts using the R packages DESeq2 (gene level) and DEXSeq (exon level). Since DESeq2 and DEXSeq requires replicates, the salmIR and salm-FRT samples were used as biological replica ...
... Table S3. Mapped sequences were run through featureCount and differential expression analysis was performed on the raw counts using the R packages DESeq2 (gene level) and DEXSeq (exon level). Since DESeq2 and DEXSeq requires replicates, the salmIR and salm-FRT samples were used as biological replica ...
Saccharopolyspora erythraea that are involved
... lying between eryAZ and eryK had an EryB phenotype. The EryC mutants, on the other hand, accumulate 3-aL-mycarosyl-erythronolide B, and convert erythromycin D (or later intermediates) to erythromycin A. These mutants cannot synthesize desosamine and/or are unable to attach it the macrolactone ring. ...
... lying between eryAZ and eryK had an EryB phenotype. The EryC mutants, on the other hand, accumulate 3-aL-mycarosyl-erythronolide B, and convert erythromycin D (or later intermediates) to erythromycin A. These mutants cannot synthesize desosamine and/or are unable to attach it the macrolactone ring. ...
Different physical delivery systems: An important approach for
... drug delivery through the skin. Among different physical carriers, electroporation and gene gun are the most potent methods for gene transfection and drug delivery in vivo. However, the researchers have focused on enhancing their potency with the structural modifications. Regarding to numerous barri ...
... drug delivery through the skin. Among different physical carriers, electroporation and gene gun are the most potent methods for gene transfection and drug delivery in vivo. However, the researchers have focused on enhancing their potency with the structural modifications. Regarding to numerous barri ...
PCR: an outstanding method
... When PCR is used only for detecting a specific DNA segment, the method is referred to as qualitative PCR. Usually the standard protocol is used. Qualitative PCR is an extremely sensitive method which is theoretically able to detect a single DNA molecule in a sample solution. In many cases specific g ...
... When PCR is used only for detecting a specific DNA segment, the method is referred to as qualitative PCR. Usually the standard protocol is used. Qualitative PCR is an extremely sensitive method which is theoretically able to detect a single DNA molecule in a sample solution. In many cases specific g ...
How Does DNA Determine the Traits of an Organism
... How Does DNA Determine the Traits of an Organism? Introduction: In this simulation, you will examine the DNA sequence of a fictitious organism the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to an ...
... How Does DNA Determine the Traits of an Organism? Introduction: In this simulation, you will examine the DNA sequence of a fictitious organism the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to an ...
Two Waves of Nuclear Factor κB Recruitment to Target Promoters
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
functional analysis of chromatin assembly genes in tetrahymena
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
Bayesian recursive mixed linear model for gene expression
... gene expression, regression coefficients had different configurations for genes 1 to 100 (β1 = λ, β2 = λ, β3 = λ, and β4 = λ), 101 to 200 (β1 = λ, β2 = 0, β3 = 0, and β4 = 0), 201 to 300 (β1 = 0, β2 = λ, β3 = 0, and β4 = 0), 301 to 400 (β1 = 0, β2 = 0, β3 = λ, and β4 = 0), 401 to 500 (β1 = 0, β2 = 0 ...
... gene expression, regression coefficients had different configurations for genes 1 to 100 (β1 = λ, β2 = λ, β3 = λ, and β4 = λ), 101 to 200 (β1 = λ, β2 = 0, β3 = 0, and β4 = 0), 201 to 300 (β1 = 0, β2 = λ, β3 = 0, and β4 = 0), 301 to 400 (β1 = 0, β2 = 0, β3 = λ, and β4 = 0), 401 to 500 (β1 = 0, β2 = 0 ...
RNA-Mediated Programming of Developmental
... variable lengths of germ line sequences. Both types of developmentally regulated deletions can be ...
... variable lengths of germ line sequences. Both types of developmentally regulated deletions can be ...
Two enhancer regions in the mouse En-2 locus
... the presumptive mid/hindbrain region of the neural epithelium (reviewed in Joyner and Hanks, 1991), suggesting that they play a role in the regional specification of the central nervous system (CNS). By analogy to their Drosophila homologue, engrailed (en) (Morata and Lawrence, 1975; Kornberg, 1981; ...
... the presumptive mid/hindbrain region of the neural epithelium (reviewed in Joyner and Hanks, 1991), suggesting that they play a role in the regional specification of the central nervous system (CNS). By analogy to their Drosophila homologue, engrailed (en) (Morata and Lawrence, 1975; Kornberg, 1981; ...
Cloning and Sequencing of a Gene from Bacillus
... The degree of homology of the DNA from different species of Bacillus has previously been studied by both interspecific transformation and DNA hybridization (Seki et al., 1975, 1979). Interspecific transformation, however, is known to be extremely inefficient (te Riele & Venema, 1982a). There is evid ...
... The degree of homology of the DNA from different species of Bacillus has previously been studied by both interspecific transformation and DNA hybridization (Seki et al., 1975, 1979). Interspecific transformation, however, is known to be extremely inefficient (te Riele & Venema, 1982a). There is evid ...
network - bioinf leipzig
... Interactions between TFs, miRNAs, other ncRNAs, and histone modifications • TFs bind as monomers, homo-dimers, or hetero-dimers • Multiple TFs (~7-10) cooperate to regulate gene expression • TFs regulate the expression of other TFs ...
... Interactions between TFs, miRNAs, other ncRNAs, and histone modifications • TFs bind as monomers, homo-dimers, or hetero-dimers • Multiple TFs (~7-10) cooperate to regulate gene expression • TFs regulate the expression of other TFs ...
Cloning and characterisation of a cysteine proteinase gene
... inhibited after incubation of gelatin-coupled gels in the presence of E-64. 3.2. Cloning of a cysteine proteinase gene (Llacys1) from L. (L.) amazonensis By using genomic DNA of L. (L.) amazonensis amastigotes and a pair of primers derived from evolutionary conserved active sites cys25 and asp145 of ...
... inhibited after incubation of gelatin-coupled gels in the presence of E-64. 3.2. Cloning of a cysteine proteinase gene (Llacys1) from L. (L.) amazonensis By using genomic DNA of L. (L.) amazonensis amastigotes and a pair of primers derived from evolutionary conserved active sites cys25 and asp145 of ...