fliD operon of Salmonella typhimurium
... the fliD gene constitutes an operon together with two additional genes, fliS and fliT. Based on the gene-disruption experiment in E. coli, both the fliS and f l i T genes have been postulated to be necessary for flagellation. In the present study, we constructed 5. typhimurium mutants in which eithe ...
... the fliD gene constitutes an operon together with two additional genes, fliS and fliT. Based on the gene-disruption experiment in E. coli, both the fliS and f l i T genes have been postulated to be necessary for flagellation. In the present study, we constructed 5. typhimurium mutants in which eithe ...
A series of promoters for constitutive expression of heterologous
... induce promoter activation. To allow constitutive expression of heterologous genes, we cloned three promoters of cam1+ , tif51+ and ef1a-c + . Construction of a series of vectors comprising these promoters and their introduction into the fission yeast cells demonstrated that the activity was differe ...
... induce promoter activation. To allow constitutive expression of heterologous genes, we cloned three promoters of cam1+ , tif51+ and ef1a-c + . Construction of a series of vectors comprising these promoters and their introduction into the fission yeast cells demonstrated that the activity was differe ...
The role of mutagenesis in defining genes in behaviour
... in specific genes, or even in gene sequences that correspond to specific functional domains in proteins.5 In addition, mutagenesis screens in other vertebrate species continue to provide alternate sources of mutant phenotypes.6 ENU mutagenesis studies are relatively simple to set up. Males are injec ...
... in specific genes, or even in gene sequences that correspond to specific functional domains in proteins.5 In addition, mutagenesis screens in other vertebrate species continue to provide alternate sources of mutant phenotypes.6 ENU mutagenesis studies are relatively simple to set up. Males are injec ...
Document
... knockdown of occludin by siRNA in tissue culture leads to an alteration in tight junction dynamics suggesting that this protein, at least in some contexts is an important tight junction modulator (Am J Physiol Cell Physiol 2005: 288:1231-1241). ...
... knockdown of occludin by siRNA in tissue culture leads to an alteration in tight junction dynamics suggesting that this protein, at least in some contexts is an important tight junction modulator (Am J Physiol Cell Physiol 2005: 288:1231-1241). ...
Characterization of the wheat gene encoding a grain
... The TaPR61 gene from bread wheat encodes a lipid transfer protein (LTP) with a hydrophobic signal peptide, predicted to direct the TaPR61 protein to the apoplast. Modelling of TaPR61 revealed the presence of an internal cavity which can accommodate at least two lipid molecules. The full-length gene, ...
... The TaPR61 gene from bread wheat encodes a lipid transfer protein (LTP) with a hydrophobic signal peptide, predicted to direct the TaPR61 protein to the apoplast. Modelling of TaPR61 revealed the presence of an internal cavity which can accommodate at least two lipid molecules. The full-length gene, ...
Identification and characterization of an early gene in the Lymantria
... This gene was subsequently designated G22, since the gene is present on the EcoRV-G fragment and is predicted to encode a protein with an M r of 22 000 (see below). The G22 transcript can be detected throughout the time course of infection (Fig. 1 a), beginning immediately after the 1 h period of vi ...
... This gene was subsequently designated G22, since the gene is present on the EcoRV-G fragment and is predicted to encode a protein with an M r of 22 000 (see below). The G22 transcript can be detected throughout the time course of infection (Fig. 1 a), beginning immediately after the 1 h period of vi ...
Tutorial: RNA-Seq Analysis Part II (Tracks): Non-Specific
... Figure 10: The dot in the scatter plot corresponding to the gene "Rps13" and the corresponding region in the trackm list "zoomed-to-selection". area showing the visible reads by grabbing at the bottom of the track's editor and dragging it downwards. You will get a view similar to that in figure 11. ...
... Figure 10: The dot in the scatter plot corresponding to the gene "Rps13" and the corresponding region in the trackm list "zoomed-to-selection". area showing the visible reads by grabbing at the bottom of the track's editor and dragging it downwards. You will get a view similar to that in figure 11. ...
S1 Document.
... QuikChange site-directed mutagenesis kit (Agilent Technologies). Primers used for mutagenesis are listed in Table S1. ...
... QuikChange site-directed mutagenesis kit (Agilent Technologies). Primers used for mutagenesis are listed in Table S1. ...
Molecular Plant-Microbe Interactions
... strain N33 (Cloutier et al. 1996a). By Southern hybridization, with the nodABC genes from Bradyrhizobium japonicum as a probe on total genomic DNA of strain N33, we showed previously that this probe hybridized with a 4.1-kb EcoRI fragment (pJC1) coding potentially for the nodA gene (Cloutier et al. ...
... strain N33 (Cloutier et al. 1996a). By Southern hybridization, with the nodABC genes from Bradyrhizobium japonicum as a probe on total genomic DNA of strain N33, we showed previously that this probe hybridized with a 4.1-kb EcoRI fragment (pJC1) coding potentially for the nodA gene (Cloutier et al. ...
20.15 Enhancers contain the same elements that are
... Enhancers are made of the same short sequence elements that are found in promoters. The density of sequence componens is greater in the enhancer than in the promoter. A difference between the enhancer and a typical promoter is presented by the density of regulatory elements. Figure 20.26 summari ...
... Enhancers are made of the same short sequence elements that are found in promoters. The density of sequence componens is greater in the enhancer than in the promoter. A difference between the enhancer and a typical promoter is presented by the density of regulatory elements. Figure 20.26 summari ...
CpG Mutation Rates in the Human Genome Are
... melt DNA (Almagor and Cole 1989). Thus, it is clear that DNA does melt reversibly (‘‘breathe’’) under physiological conditions, and this melting must have an effect on the rates of cytosine deamination. The question then becomes the quantitative extent to which DNA melting (as opposed to other proce ...
... melt DNA (Almagor and Cole 1989). Thus, it is clear that DNA does melt reversibly (‘‘breathe’’) under physiological conditions, and this melting must have an effect on the rates of cytosine deamination. The question then becomes the quantitative extent to which DNA melting (as opposed to other proce ...
Regulation of phenylalanine biosynthesis in Escherichia coli K
... Bacteriophage X ppheA-lac was used to obtain strains of Escherichia coli K-12 in which pheA and lacZ are each transcribed from a separate pheA promoter. Mutants in which both 3-galactosidase and chorismate mutase P-prephenate dehydratase (the pheA gene product) were derepressed were isolated, and a ...
... Bacteriophage X ppheA-lac was used to obtain strains of Escherichia coli K-12 in which pheA and lacZ are each transcribed from a separate pheA promoter. Mutants in which both 3-galactosidase and chorismate mutase P-prephenate dehydratase (the pheA gene product) were derepressed were isolated, and a ...
About OMICS Group
... Total of 618 differentially expressed miRNAs were detected between Brassica hexaploid and its parents. More differentially expressed miRNAs with larger differences in expression existed between Brassica hexaploid and B. rapa, which is consistent with the progenitor-biased gene repression in this h ...
... Total of 618 differentially expressed miRNAs were detected between Brassica hexaploid and its parents. More differentially expressed miRNAs with larger differences in expression existed between Brassica hexaploid and B. rapa, which is consistent with the progenitor-biased gene repression in this h ...
Neurobiology of Learning and Memory
... Tardif, 1992; Cho et al., 1995) suggests the existence of learning- and memory-related functions in these areas, and the three systems dealt with here undoubtedly interact with these substrates. It is also sometimes suggested that plasticity is a general property of the nervous system, and that expe ...
... Tardif, 1992; Cho et al., 1995) suggests the existence of learning- and memory-related functions in these areas, and the three systems dealt with here undoubtedly interact with these substrates. It is also sometimes suggested that plasticity is a general property of the nervous system, and that expe ...
Nucleotide Sequence and Organization of the Rat Heme Oxygenase
... binding site for Spl (reviewed in Ref. 31), are located at G A C C T 1 2 positions (-520) to (-526), (-99) to (-91) and (-58) to (-52). The first of these three is in a 3’ to 5’ orientation. The second sequence,GGGGCGGGG,is completely identical with the consensus sequences of Spl binding sites (33). ...
... binding site for Spl (reviewed in Ref. 31), are located at G A C C T 1 2 positions (-520) to (-526), (-99) to (-91) and (-58) to (-52). The first of these three is in a 3’ to 5’ orientation. The second sequence,GGGGCGGGG,is completely identical with the consensus sequences of Spl binding sites (33). ...
Interplay between copy number, dosage compensation and
... unlike the situation observed in single cell expression noise experiments, we found no statistically significant negative correlation between and expression level (Fig S1). Therefore, to examine quantitatively the effect of gene dose reduction on expression variability, we pooled values for all ...
... unlike the situation observed in single cell expression noise experiments, we found no statistically significant negative correlation between and expression level (Fig S1). Therefore, to examine quantitatively the effect of gene dose reduction on expression variability, we pooled values for all ...
as a PDF
... acetylation pattern of histone H4, in male germline cells. Although MLE is known to be involved in X chromosome hypertranscription in somatic cells, presumably through the recruitment of histone acetylation factors [31,32], it does not associate specifically with the X chromosome in male germ cells. ...
... acetylation pattern of histone H4, in male germline cells. Although MLE is known to be involved in X chromosome hypertranscription in somatic cells, presumably through the recruitment of histone acetylation factors [31,32], it does not associate specifically with the X chromosome in male germ cells. ...
Mining Phenotypes and Informative Genes from Gene Expression
... If phenotype information is known, the major task is to select the informative genes that manifest the phenotypes of samples. This can be achieved by supervised analysis methods such as the neighborhood analysis [8] and the support vector machine [4]. Although the supervised methods are helpful, the ...
... If phenotype information is known, the major task is to select the informative genes that manifest the phenotypes of samples. This can be achieved by supervised analysis methods such as the neighborhood analysis [8] and the support vector machine [4]. Although the supervised methods are helpful, the ...
splicing
... • Individual genes can be silenced in mammals by RNAi that targets the gene’s control region, a promoter-associated transcript) rather than the coding region • Another silencing mechanism targets nuclear RNA as endogenous double-stranded siRNAs that contain a nuclear localization signal can enter th ...
... • Individual genes can be silenced in mammals by RNAi that targets the gene’s control region, a promoter-associated transcript) rather than the coding region • Another silencing mechanism targets nuclear RNA as endogenous double-stranded siRNAs that contain a nuclear localization signal can enter th ...
Transgenic mice overexpressing the full
... (Ribases et al., 2005; Liu et al., 2005). Administration of brainderived neurotrophic factor (BDNF- Mouse Genome Informatics) or neurotrophin-3 (NT3; NTF3-Mouse Genome Informatics) into the dentate gyrus or CA3 region of hippocampus causes antidepressantlike effects in the forced swim and learned he ...
... (Ribases et al., 2005; Liu et al., 2005). Administration of brainderived neurotrophic factor (BDNF- Mouse Genome Informatics) or neurotrophin-3 (NT3; NTF3-Mouse Genome Informatics) into the dentate gyrus or CA3 region of hippocampus causes antidepressantlike effects in the forced swim and learned he ...
Methylation of the Factor IX Gene is the Main Source of Mutations
... replicative DNA methylation and by misrepairing G:T pairs. Such processes may be one of the main sources of mutations in this gene, which repeatedly occur de novo and support the incidence of hemophilia В with a high frequency. Asymmetry of C T and GA transition mutations was found in a number of ...
... replicative DNA methylation and by misrepairing G:T pairs. Such processes may be one of the main sources of mutations in this gene, which repeatedly occur de novo and support the incidence of hemophilia В with a high frequency. Asymmetry of C T and GA transition mutations was found in a number of ...
MUSHROOM BODY MEMOIR: FROM MAPS TO MODELS
... more extensively, and this might occur in the mushroom bodies. Imagine that each Kenyon cell is connected to three projection neurons and that every possible combination of three projection neurons has a Kenyon cell. Let us also assume that Kenyon cells are coincidence detectors that generate an act ...
... more extensively, and this might occur in the mushroom bodies. Imagine that each Kenyon cell is connected to three projection neurons and that every possible combination of three projection neurons has a Kenyon cell. Let us also assume that Kenyon cells are coincidence detectors that generate an act ...
Regulation of secondary metabolism in streptomycetes
... separated by over 100 kb of DNA in S. griseus [31], their homologues in S. virginiae and S. lavendulae are juxtaposed, and in S. virginiae they lie between the two secondary metabolic gene clusters that they regulate (those for virginiamycin M and virginiamycin S [27]). Similarly, although initiall ...
... separated by over 100 kb of DNA in S. griseus [31], their homologues in S. virginiae and S. lavendulae are juxtaposed, and in S. virginiae they lie between the two secondary metabolic gene clusters that they regulate (those for virginiamycin M and virginiamycin S [27]). Similarly, although initiall ...
Impact of genetic engineering on the understanding of
... ®elds. Of particular interest is the control of transgene expression by endogenous regulatory sequence of the gene of interest (socalled knock-in). The latter enables the study of gene complementation, such as a possible rescue of a disease by a member of the same gene family. Gene-trap mutagenesis ...
... ®elds. Of particular interest is the control of transgene expression by endogenous regulatory sequence of the gene of interest (socalled knock-in). The latter enables the study of gene complementation, such as a possible rescue of a disease by a member of the same gene family. Gene-trap mutagenesis ...