Enolase and Glycolytic Flux Play a Role in the Regulation
... in the double-mutant ⌬Sceno1⌬Sceno2 since it is inviable on glucose or glycerol media (see below). KlENO transcription was examined by a Northern blot analysis. The level of the KlENO transcript is slightly higher (twofold) when the cells are grown on 2% glucose (Figure 3B) than when they are cultiv ...
... in the double-mutant ⌬Sceno1⌬Sceno2 since it is inviable on glucose or glycerol media (see below). KlENO transcription was examined by a Northern blot analysis. The level of the KlENO transcript is slightly higher (twofold) when the cells are grown on 2% glucose (Figure 3B) than when they are cultiv ...
Imprinting capacity of gamete lineages in C. elegans
... populations of animals, the only difference being the parental source of the reporter chromosome (sperm versus oocyte) and the paired or unpaired character of the reporter-carrying chromosome in the parental generation. Measurement of relative GFP levels between two populations of animals presents a ...
... populations of animals, the only difference being the parental source of the reporter chromosome (sperm versus oocyte) and the paired or unpaired character of the reporter-carrying chromosome in the parental generation. Measurement of relative GFP levels between two populations of animals presents a ...
Transcriptional Repression of the
... DBD (D538–614) or the LBD (D660–919) eliminated AR-dependent repression, indicating that these two domains are critical. The requirement for the DBD was further substantiated using a point mutant that has a cysteine-to-alanine mutation within the first zinc finger (C576A). This mutation, which disru ...
... DBD (D538–614) or the LBD (D660–919) eliminated AR-dependent repression, indicating that these two domains are critical. The requirement for the DBD was further substantiated using a point mutant that has a cysteine-to-alanine mutation within the first zinc finger (C576A). This mutation, which disru ...
Mice lacking desmocollin 1 show epidermal fragility accompanied
... cells often but not always appeared larger in the thickened null epidermis (Fig. 5, A and B, compared with Fig. 3, E and F). To determine whether epidermal thickening was due to increased cell proliferation, we examined the skin from null and normal mice using an antibody against Ki67. Proliferating ...
... cells often but not always appeared larger in the thickened null epidermis (Fig. 5, A and B, compared with Fig. 3, E and F). To determine whether epidermal thickening was due to increased cell proliferation, we examined the skin from null and normal mice using an antibody against Ki67. Proliferating ...
Forkhead Transcription Factors: Key Players in Development and
... and mode of DNA recognition within the forkhead family. This has been confirmed by NMR structural analysis of the DNA binding domains of three additional forkhead proteins: FOXC2, Foxd3, and FOXO4 (Jin et al., 1999; Marsden et al., 1998; van Dongen et al., 2000; Weigelt et al., 2001). While Clark et ...
... and mode of DNA recognition within the forkhead family. This has been confirmed by NMR structural analysis of the DNA binding domains of three additional forkhead proteins: FOXC2, Foxd3, and FOXO4 (Jin et al., 1999; Marsden et al., 1998; van Dongen et al., 2000; Weigelt et al., 2001). While Clark et ...
PPT - Bioinformatics.ca
... • Terms can have more than one parent or child Module 1: Introduction to Gene Lists ...
... • Terms can have more than one parent or child Module 1: Introduction to Gene Lists ...
Disruption of an N-acetyltransferase gene in the
... according to published literature (True, 2003; Wittkopp et al., 2003a; Arakane et al., 2009)] is illustrated in the center (see Discussion). (A)Adult wildtype (+/+) and mln mutant (mln/mln) Bombyx mori. (B)Regional pigmentation phenotypes of mln and wild-type larvae. In mln, the head and forelegs ...
... according to published literature (True, 2003; Wittkopp et al., 2003a; Arakane et al., 2009)] is illustrated in the center (see Discussion). (A)Adult wildtype (+/+) and mln mutant (mln/mln) Bombyx mori. (B)Regional pigmentation phenotypes of mln and wild-type larvae. In mln, the head and forelegs ...
Experimental and Molecular Pathology
... and has been shown to be necessary for the development of the mature thymic epithelium (Blackburn et al., 1996; Brisette et al., 1996; Lee et al., 1999). Expression of the Foxn1 gene, the subsequent Foxn1 mRNA, and the FOXN1 protein can be detected as early as day 13 of gestation in the developing n ...
... and has been shown to be necessary for the development of the mature thymic epithelium (Blackburn et al., 1996; Brisette et al., 1996; Lee et al., 1999). Expression of the Foxn1 gene, the subsequent Foxn1 mRNA, and the FOXN1 protein can be detected as early as day 13 of gestation in the developing n ...
The role of variable DNA tandem repeats in bacterial adaptation
... to rearrange and cause frameshift mutations in genes (Coenye & Vandamme, 2005; Ackermann & Chao, 2006; Orsi et al., 2010; Lin & Kussell, 2012). In contrast, SSRs whose unit size is a multiple of three nucleotides (3, 6, 9 …) are overrepresented in open reading frames (ORFs) because their expansion o ...
... to rearrange and cause frameshift mutations in genes (Coenye & Vandamme, 2005; Ackermann & Chao, 2006; Orsi et al., 2010; Lin & Kussell, 2012). In contrast, SSRs whose unit size is a multiple of three nucleotides (3, 6, 9 …) are overrepresented in open reading frames (ORFs) because their expansion o ...
Gene Codon Composition Determines Differentiation
... monoclonal antibody against PV1 L1 protein (67), followed by Cy3-conjugated anti-mouse IgG (Sigma). L1-labeled KCs were further blocked with 5% skim milk–PBS and probed with fluorescein isothiocyanate-conjugated monoclonal antibody against keratin 14 (K14; Covance). Fixed KCs were probed with antibo ...
... monoclonal antibody against PV1 L1 protein (67), followed by Cy3-conjugated anti-mouse IgG (Sigma). L1-labeled KCs were further blocked with 5% skim milk–PBS and probed with fluorescein isothiocyanate-conjugated monoclonal antibody against keratin 14 (K14; Covance). Fixed KCs were probed with antibo ...
Comparison of Statistical Models for Affymetrix GeneChip
... Model-based estimators are superior to simple averaging Full model superior to reduced this does not necessarily mean that the mismatch probes are a good idea - but if they are present we should use them we have demonstrated this using both analytic considerations and experimental data a carefully d ...
... Model-based estimators are superior to simple averaging Full model superior to reduced this does not necessarily mean that the mismatch probes are a good idea - but if they are present we should use them we have demonstrated this using both analytic considerations and experimental data a carefully d ...
Inheritance of Red Green - Department Of Biological Sciences
... variants, available evidence points to allelism of those traits that affect a given cone type. However, a true complementation test (requiring expression of both alleles in the same cell) is not possible because each cell in a female expresses only one of her two X chromosomes (6). The evidence for ...
... variants, available evidence points to allelism of those traits that affect a given cone type. However, a true complementation test (requiring expression of both alleles in the same cell) is not possible because each cell in a female expresses only one of her two X chromosomes (6). The evidence for ...
The role of regulatory variation in complex traits and
... eQTLs are located near the genes they influence, whereas others are located elsewhere in the genome3. The former have been called ‘local’ eQTLs13. Local eQTLs can influence gene expression by two different mechanisms. Most obviously, they can act in cis and affect expression in an allele-specific ma ...
... eQTLs are located near the genes they influence, whereas others are located elsewhere in the genome3. The former have been called ‘local’ eQTLs13. Local eQTLs can influence gene expression by two different mechanisms. Most obviously, they can act in cis and affect expression in an allele-specific ma ...
Cell type-specific activation of p38 MAPK in the brain regions of
... 2005). Briefly, 50 mg of protein from the whole tissue homogenate of each sample was loaded in 10% SDS–PAGE gel. Then, the proteins were transferred onto the nitrocellulose membrane (NC membrane, Bio-Rad, USA) at 4 8C, 400 mA for 4 h. The transferred NC membrane was washed for 10 min with TTBS (20 m ...
... 2005). Briefly, 50 mg of protein from the whole tissue homogenate of each sample was loaded in 10% SDS–PAGE gel. Then, the proteins were transferred onto the nitrocellulose membrane (NC membrane, Bio-Rad, USA) at 4 8C, 400 mA for 4 h. The transferred NC membrane was washed for 10 min with TTBS (20 m ...
Plxdc2/TEM7R of the mouse
... Gene Expression Patterns xxx (2007) xxx–xxx www.elsevier.com/locate/modgep ...
... Gene Expression Patterns xxx (2007) xxx–xxx www.elsevier.com/locate/modgep ...
Hepatocyte-Cfh -/- mice have reduced plasma FH and C3
... (supplemental Figures 4-6). CfhloxP/loxP mice, in which we had demonstrated slight reduction in plasma FH levels (Figure 1B), developed a phenotype intermediate in severity between wild-type and hepatocyte-Cfh-/- mice. Together these data indicated that sub-total FH deficiency was associated with a ...
... (supplemental Figures 4-6). CfhloxP/loxP mice, in which we had demonstrated slight reduction in plasma FH levels (Figure 1B), developed a phenotype intermediate in severity between wild-type and hepatocyte-Cfh-/- mice. Together these data indicated that sub-total FH deficiency was associated with a ...
The Advantage and Application of Genetically Humanized Mouse
... mice carrying an actively expressed human growth hormone gene9. Since these early reports, thousands of transgenic mice have been generated and characterized to define the function of human genes in normal development and disease pathogenesis. Transgenic technology has been widely used as a method t ...
... mice carrying an actively expressed human growth hormone gene9. Since these early reports, thousands of transgenic mice have been generated and characterized to define the function of human genes in normal development and disease pathogenesis. Transgenic technology has been widely used as a method t ...
PDF - Blood Journal
... Surprisingly, the genes have split onto separate chromosomes in the pufferfish, as demonstrated by DNA–fluorescence in situ hybridization (FISH) analysis. Sequence analysis of the cosmid containing the -globin gene reveals close linkage with 2 ␣-globin genes, ␣3 and ␣4; we refer to this locus as th ...
... Surprisingly, the genes have split onto separate chromosomes in the pufferfish, as demonstrated by DNA–fluorescence in situ hybridization (FISH) analysis. Sequence analysis of the cosmid containing the -globin gene reveals close linkage with 2 ␣-globin genes, ␣3 and ␣4; we refer to this locus as th ...
Copy Number and Gene Expression Integration in Partek
... arrays were simultaneously imported into Partek and copy number estimates were created using the normal pair for each sample as the normal baseline. The resulting paired copy number estimate file is the first file in the copy number section. The intensity copy number sheet is not included in the dat ...
... arrays were simultaneously imported into Partek and copy number estimates were created using the normal pair for each sample as the normal baseline. The resulting paired copy number estimate file is the first file in the copy number section. The intensity copy number sheet is not included in the dat ...
Turning floral organs into leaves, leaves into floral organs Koji Goto
... was identified as another MADS-box gene, AGL9 (agamous-like 9) [42••,46]. Other kinds of proteins were not isolated in the yeast two-hybrid screening, suggesting that the entire complex may be composed solely of MADS-box proteins, and that AGL9 may supply a transcriptional activator domain. Notably, ...
... was identified as another MADS-box gene, AGL9 (agamous-like 9) [42••,46]. Other kinds of proteins were not isolated in the yeast two-hybrid screening, suggesting that the entire complex may be composed solely of MADS-box proteins, and that AGL9 may supply a transcriptional activator domain. Notably, ...
nar-02451-data-e-201
... We implemented a post-processing step to extract the species information. The post-processing is performed after an association between a methylated gene and a disease is extracted from a sentence by searching for the species names in the sentence. However, we found that genes, diseases, species, an ...
... We implemented a post-processing step to extract the species information. The post-processing is performed after an association between a methylated gene and a disease is extracted from a sentence by searching for the species names in the sentence. However, we found that genes, diseases, species, an ...
Package `TCGAbiolinks`
... query <- GDCquery(project = "TCGA-COAD", data.category = "Clinical", barcode = c("TCGA-RU-A8FL","TCGA-AA-3972")) GDCdownload(query) clinical <- GDCprepare_clinic(query,"patient") clinical.drug <- GDCprepare_clinic(query,"drug") clinical.radiation <- GDCprepare_clinic(query,"radiation") clinical.admi ...
... query <- GDCquery(project = "TCGA-COAD", data.category = "Clinical", barcode = c("TCGA-RU-A8FL","TCGA-AA-3972")) GDCdownload(query) clinical <- GDCprepare_clinic(query,"patient") clinical.drug <- GDCprepare_clinic(query,"drug") clinical.radiation <- GDCprepare_clinic(query,"radiation") clinical.admi ...
not a plastid specific promoter but is also capable of
... The two cocultivation experiments yielded an equivalent number of resistant calli. The expression capability of the 35S nptH gene is well documented (10). Therefore, the comparable transformation efficiencies suggest that in the case of the SRI plants transformed by pGSC54 the nptH gene is driven by ...
... The two cocultivation experiments yielded an equivalent number of resistant calli. The expression capability of the 35S nptH gene is well documented (10). Therefore, the comparable transformation efficiencies suggest that in the case of the SRI plants transformed by pGSC54 the nptH gene is driven by ...
Towards identifying the full set of genes involved in post
... vital staple food to over 500 million in the humid tropics. Unfortunately, it suffers from a rapid post-harvest physiological deterioration (PPD) that can render the roots uneatable and unmarketable within 24-72 hours of harvest. Increased urbanization has lengthened the distance and time between fa ...
... vital staple food to over 500 million in the humid tropics. Unfortunately, it suffers from a rapid post-harvest physiological deterioration (PPD) that can render the roots uneatable and unmarketable within 24-72 hours of harvest. Increased urbanization has lengthened the distance and time between fa ...
A Major Species of Mouse μ-opioid Receptor mRNA and Its
... also been defined by other labs, including STAT6 (Kraus et al., 2001), cAMP response element-binding protein (Lee and Lee, 2003), and neurorestrictive suppressor element (Andria and Simon, 2001). However, studies of its 3⬘-UTR have been very limited. Ample evidence has shown that 3⬘-UTR of mRNA play ...
... also been defined by other labs, including STAT6 (Kraus et al., 2001), cAMP response element-binding protein (Lee and Lee, 2003), and neurorestrictive suppressor element (Andria and Simon, 2001). However, studies of its 3⬘-UTR have been very limited. Ample evidence has shown that 3⬘-UTR of mRNA play ...