![Virus induced gene silencing, a post transcriptional gene silencing](http://s1.studyres.com/store/data/009138581_1-c4038e02430e77d313bc2104ab95a30d-300x300.png)
Virus induced gene silencing, a post transcriptional gene silencing
... after 7-10 days post inoculation (dpi). Leaves from inoculated plants are collected after approximately 14-15 day post inoculation (dpi) in order to check pds gene silencing level by qRTPCR [27, 38]. Improvements of virus-induced gene silencing Gene specific silencing via VIGS system is now used for ...
... after 7-10 days post inoculation (dpi). Leaves from inoculated plants are collected after approximately 14-15 day post inoculation (dpi) in order to check pds gene silencing level by qRTPCR [27, 38]. Improvements of virus-induced gene silencing Gene specific silencing via VIGS system is now used for ...
Induction of XIST expression from the human active
... chromosome has been shown to be methylated at a series of restriction enzyme sites near the 5′ end of the gene (22). We used a PCR-based assay to examine methylation at a subset of these sites (Fig. 2). Primers AT-2 and 29r amplify DNA from the 5′ flanking region into the first exon of XIST generati ...
... chromosome has been shown to be methylated at a series of restriction enzyme sites near the 5′ end of the gene (22). We used a PCR-based assay to examine methylation at a subset of these sites (Fig. 2). Primers AT-2 and 29r amplify DNA from the 5′ flanking region into the first exon of XIST generati ...
Protein A gene expression is regulated by DNA supercoiling which
... supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, in E. coli, factors responsible for modifying DNA supercoiling fall into three general categories: those affecting DNA topoisomerases, those altering DNA structure, and tho ...
... supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, in E. coli, factors responsible for modifying DNA supercoiling fall into three general categories: those affecting DNA topoisomerases, those altering DNA structure, and tho ...
The Perirhinal, Entorhinal, and Parahippocampal Cortices and
... suggested that the PRc represents the apex of the ventral occipitaltemporal visual processing pathway, which computes increasingly more complex combinations of visual features from posterior to anterior sites. Thus, the PRc may be enanged during demanding visual perceptual task, e.g. discriminating ...
... suggested that the PRc represents the apex of the ventral occipitaltemporal visual processing pathway, which computes increasingly more complex combinations of visual features from posterior to anterior sites. Thus, the PRc may be enanged during demanding visual perceptual task, e.g. discriminating ...
Genetic interaction of Lhx4 and Prop1 - Development
... 4230 L. T. Raetzman, R. Ward and S. A. Camper Classical genetic analysis of the LIM gene family reveals similar roles to those of the PITX family (Sheng et al., 1997; Sheng et al., 1996). Both Lhx3 and Lhx4 are expressed in Rathke’s pouch. The Lhx3-deficient phenotype is similar to that of Pitx2, c ...
... 4230 L. T. Raetzman, R. Ward and S. A. Camper Classical genetic analysis of the LIM gene family reveals similar roles to those of the PITX family (Sheng et al., 1997; Sheng et al., 1996). Both Lhx3 and Lhx4 are expressed in Rathke’s pouch. The Lhx3-deficient phenotype is similar to that of Pitx2, c ...
Temporal and Spatial Expression of Homeotic Genes Is Important for
... (Hosoya et al., 1995), and differentially regulates the expression of over 400 genes (Egger et al., 2002). gcm also regulates the expression of genes, such as, reverse polarity (repo) (Halter et al., 1995; Xiong et al., 1994), which encodes a homeodomain protein and is expressed exclusively in almos ...
... (Hosoya et al., 1995), and differentially regulates the expression of over 400 genes (Egger et al., 2002). gcm also regulates the expression of genes, such as, reverse polarity (repo) (Halter et al., 1995; Xiong et al., 1994), which encodes a homeodomain protein and is expressed exclusively in almos ...
Genetic interaction of Lhx4 and Prop1
... 4230 L. T. Raetzman, R. Ward and S. A. Camper Classical genetic analysis of the LIM gene family reveals similar roles to those of the PITX family (Sheng et al., 1997; Sheng et al., 1996). Both Lhx3 and Lhx4 are expressed in Rathke’s pouch. The Lhx3-deficient phenotype is similar to that of Pitx2, c ...
... 4230 L. T. Raetzman, R. Ward and S. A. Camper Classical genetic analysis of the LIM gene family reveals similar roles to those of the PITX family (Sheng et al., 1997; Sheng et al., 1996). Both Lhx3 and Lhx4 are expressed in Rathke’s pouch. The Lhx3-deficient phenotype is similar to that of Pitx2, c ...
Analysis of DNA transcription termination sequences of gene coding
... Pseudomonas strains was investigated (Figure 2). These regions are vastly different. The length of this region ranged from 60 bp (Pseudomonas putida AF150670) to 225 bp (Pseudomonas corrugata AY910767). In case of Pseudomonas USM4-55, Pseudomonas nitroreducens and Pseudomonas pseudoalcaligenes the s ...
... Pseudomonas strains was investigated (Figure 2). These regions are vastly different. The length of this region ranged from 60 bp (Pseudomonas putida AF150670) to 225 bp (Pseudomonas corrugata AY910767). In case of Pseudomonas USM4-55, Pseudomonas nitroreducens and Pseudomonas pseudoalcaligenes the s ...
1 - Projeto Andar de Novo
... the recombinant human protein rhPIF/DCD against lesion caused as consequence of status epilepticus (SE) induced by kainic acid in rats (15 mg/kg, i.p.). Our results show that pretreatment with rhPIF/DCD (30-260 g/kg, i.v.) decreases significantly the severity of the SE. Besides, a significant reduct ...
... the recombinant human protein rhPIF/DCD against lesion caused as consequence of status epilepticus (SE) induced by kainic acid in rats (15 mg/kg, i.p.). Our results show that pretreatment with rhPIF/DCD (30-260 g/kg, i.v.) decreases significantly the severity of the SE. Besides, a significant reduct ...
Module 8: Horizontal Gene Transfer
... show an ancestral lineage. An unrooted person's family tree would show clusters of blood relatives, but would not show who was ancestor or progeny in those clusters. The T-Coffee website that you have already used in the Sequence Based Similarity Module allows for the construction of basic cladogram ...
... show an ancestral lineage. An unrooted person's family tree would show clusters of blood relatives, but would not show who was ancestor or progeny in those clusters. The T-Coffee website that you have already used in the Sequence Based Similarity Module allows for the construction of basic cladogram ...
Supplementary Material PDF
... Fig. S3. Double fluorescent ISH shows mosaic colocalization of enhancer:GFP with endogenous foxd3 and tfap2a mRNA expression. (A-F) Double fluorescent ISH for gfp (A) and foxd3 (B) in foxd3E1:GFP-injected embryos (merge in C, lateral view) and for gfp (D) and tfap2a (E) in tfap2aE2:GFP-injected emb ...
... Fig. S3. Double fluorescent ISH shows mosaic colocalization of enhancer:GFP with endogenous foxd3 and tfap2a mRNA expression. (A-F) Double fluorescent ISH for gfp (A) and foxd3 (B) in foxd3E1:GFP-injected embryos (merge in C, lateral view) and for gfp (D) and tfap2a (E) in tfap2aE2:GFP-injected emb ...
Recruitment of Drosophila Polycomb Group proteins to
... can only be observed when Ab-Fab is in a homozygous state (Fig. 2b), suggesting that somatic pairing of homologous chromosomes, a phenomenon known for its influence on homeotic gene expression in Drosophila18, might affect trxG-dependent regulation. These data indicate that mutation of the G(A) moti ...
... can only be observed when Ab-Fab is in a homozygous state (Fig. 2b), suggesting that somatic pairing of homologous chromosomes, a phenomenon known for its influence on homeotic gene expression in Drosophila18, might affect trxG-dependent regulation. These data indicate that mutation of the G(A) moti ...
Loss of cell adhesion molecule CHL1 improves homeostatic
... Close homologue of L1 (CHL1) is a transmembrane cell adhesion molecule that is critical for brain development and for the maintenance of neural circuits in adults. Recent studies revealed that CHL1 has diverse roles and is involved in the regulation of recovery after spinal cord injury. CHL1 express ...
... Close homologue of L1 (CHL1) is a transmembrane cell adhesion molecule that is critical for brain development and for the maintenance of neural circuits in adults. Recent studies revealed that CHL1 has diverse roles and is involved in the regulation of recovery after spinal cord injury. CHL1 express ...
5 Genetic Analysis of Kidney Disease in Mice
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
Chapter 27 Phage Strategies
... 27.17 Lysogeny Requires Several Events • cII and cIII cause repressor synthesis to be established and also trigger inhibition of late gene transcription. • Establishment of repressor turns off immediate and delayed early gene expression. • Repressor turns on the maintenance circuit for its own synt ...
... 27.17 Lysogeny Requires Several Events • cII and cIII cause repressor synthesis to be established and also trigger inhibition of late gene transcription. • Establishment of repressor turns off immediate and delayed early gene expression. • Repressor turns on the maintenance circuit for its own synt ...
Cloning and functional analysis of the chitinase gene promoter in
... pathogens (Kellmann et al., 1996; Liu et al., 2007). Production of chitinase can be induced in plants by inoculation with pathogenic fungi and by exogenous application of salicylic acid (SA) (Kellmann et al., 1996; Qu et al., 2008). The successful creation of transgenic plants with the required leve ...
... pathogens (Kellmann et al., 1996; Liu et al., 2007). Production of chitinase can be induced in plants by inoculation with pathogenic fungi and by exogenous application of salicylic acid (SA) (Kellmann et al., 1996; Qu et al., 2008). The successful creation of transgenic plants with the required leve ...
Imprinted Genes and Human Disease
... particularly fragile. That is, are they more likely to undergo mutation and/or are mutations of imprinted genes particularly likely to result in human disease? In general we consider how the field of evolutionary medicine—the use of evolution to understand why our body’s design allows for the existe ...
... particularly fragile. That is, are they more likely to undergo mutation and/or are mutations of imprinted genes particularly likely to result in human disease? In general we consider how the field of evolutionary medicine—the use of evolution to understand why our body’s design allows for the existe ...
Ends-out, or replacement, gene targeting in Drosophila
... the same as those used for ends-in targeting: heat-inducible FLP recombinase (13) and I-SceI endonuclease (3) transgenes (70FLP and 70I-SceI). We also constructed donor transgenes that carry sequence from the locus to be targeted. We first made and transformed a y⫹ P element donor construct to rescu ...
... the same as those used for ends-in targeting: heat-inducible FLP recombinase (13) and I-SceI endonuclease (3) transgenes (70FLP and 70I-SceI). We also constructed donor transgenes that carry sequence from the locus to be targeted. We first made and transformed a y⫹ P element donor construct to rescu ...
Pathogen Response Genes Mediate Caenorhabditis elegans Innate
... worms fed non-pathogenic E. coli (OP50) (Troemel et al. 2006). To explore the targets of the infection receptor FSHR-1, Powell et al. (2009) performed Pseudomonas-specific microarrays on fshr-1 null mutant worms compared to wild type (N2) worms. Interestingly, 179 genes were found to be upregulated ...
... worms fed non-pathogenic E. coli (OP50) (Troemel et al. 2006). To explore the targets of the infection receptor FSHR-1, Powell et al. (2009) performed Pseudomonas-specific microarrays on fshr-1 null mutant worms compared to wild type (N2) worms. Interestingly, 179 genes were found to be upregulated ...
Chromosome-encoded gene cluster for the
... from the total DNA of strain AD9 into Escherichia coli JM109. After shotgun cloning, two recombinant E. coli strains showing aniline oxidation activity or catechol meta-cleavage activity were obtained by simple plate assays. These strains contained 9?3 kb and 15?4 kb DNA fragments, respectively. Seq ...
... from the total DNA of strain AD9 into Escherichia coli JM109. After shotgun cloning, two recombinant E. coli strains showing aniline oxidation activity or catechol meta-cleavage activity were obtained by simple plate assays. These strains contained 9?3 kb and 15?4 kb DNA fragments, respectively. Seq ...
Reciprocal products of chromosomal translocations in human
... leukaemia (CML), in which the reciprocal product ABL–BCR is detected in ~60% of the cases [11], and patients with acute promyelocytic leukaemia (APL), in which both products are detected in the vast majority of cases [14,15]. Although for CML there is no direct experimental evidence that the recipro ...
... leukaemia (CML), in which the reciprocal product ABL–BCR is detected in ~60% of the cases [11], and patients with acute promyelocytic leukaemia (APL), in which both products are detected in the vast majority of cases [14,15]. Although for CML there is no direct experimental evidence that the recipro ...
The Mouse Immunoglobulin Heavy Chain V
... other element identified in the V-D-J region, the PDQ52 promoter/enhancer, is unlikely to play a role because its deletion does not affect germ line V gene transcription (19) or V to DJ recombination (20). Furthermore, the large V region (2.5 Mb), contains 195 V genes (500 bp) separated by intergeni ...
... other element identified in the V-D-J region, the PDQ52 promoter/enhancer, is unlikely to play a role because its deletion does not affect germ line V gene transcription (19) or V to DJ recombination (20). Furthermore, the large V region (2.5 Mb), contains 195 V genes (500 bp) separated by intergeni ...
Adverse Effects of Excessive Leucine Intake Depend on Dietary
... adverse effects of excessive leucine (Leu) in Sprague-Dawley rats by DNA microarray. It has long been known that the adverse effects of excessive amino acid intake depend on dietary protein levels. Male rats were divided into 12 groups (n56) and fed for 1 wk a diet containing low (6%), moderate (12% ...
... adverse effects of excessive leucine (Leu) in Sprague-Dawley rats by DNA microarray. It has long been known that the adverse effects of excessive amino acid intake depend on dietary protein levels. Male rats were divided into 12 groups (n56) and fed for 1 wk a diet containing low (6%), moderate (12% ...
Document
... The OxyS RNA is a short sequence (109 nt) that does not code for protein. It is a trans-acting regulator that affects gene expression at posttranscriptional levels. It has >10 target loci; at some of them, it activates expression; at others it represses expression. Fig. 13.18 shows the mechanism ...
... The OxyS RNA is a short sequence (109 nt) that does not code for protein. It is a trans-acting regulator that affects gene expression at posttranscriptional levels. It has >10 target loci; at some of them, it activates expression; at others it represses expression. Fig. 13.18 shows the mechanism ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... generates two samples that need analysis by capillary electrophoresis: one undigested sample for copy number detection and one digested sample for methylation detection. More information about MS-MLPA can be found on page 2 and in the MS-MLPA protocol. The MS-MLPA probes in this ME003-A1 probemix de ...
... generates two samples that need analysis by capillary electrophoresis: one undigested sample for copy number detection and one digested sample for methylation detection. More information about MS-MLPA can be found on page 2 and in the MS-MLPA protocol. The MS-MLPA probes in this ME003-A1 probemix de ...