![Involvement of Pax6 and Otx2 in the forebrain](http://s1.studyres.com/store/data/006806707_1-ab8241934c71713b6895b8bf0bcb293e-300x300.png)
Involvement of Pax6 and Otx2 in the forebrain
... In this study, we describe the involvement of the homeodomain containing transcription factors Otx2 and Pax6 in ANF gene regulation. Otx2 is known for its specific function as a transcriptional regulator during forebrain and midbrain development in vertebrates (Boncinelli and Morgan, 2001). In all e ...
... In this study, we describe the involvement of the homeodomain containing transcription factors Otx2 and Pax6 in ANF gene regulation. Otx2 is known for its specific function as a transcriptional regulator during forebrain and midbrain development in vertebrates (Boncinelli and Morgan, 2001). In all e ...
cellular mechanisms of classical and operant conditioning A model
... by CS test stimuli, which were delivered prior to training (pretest) and 1 or 24 h after training (post-test). At both time points after training, paired training produced a significant increase in the number of bites (post-test minus pre-test) as compared to unpaired training (Fig. 3). These data i ...
... by CS test stimuli, which were delivered prior to training (pretest) and 1 or 24 h after training (post-test). At both time points after training, paired training produced a significant increase in the number of bites (post-test minus pre-test) as compared to unpaired training (Fig. 3). These data i ...
Rolling circle transcription on smallest size double stranded DNA
... chemical degradation synthetic nucleic acid analogs with similar base pairing properties to DNA may be employed. Examples include peptic nucleic acid, PNA, locked nucleic acid, LNA and 2’ O-methyl modified RNA (Egholm, et al. 1993) (Kaur, et al. 2006) (Sproat, et al. 1989). Functionalization Synthet ...
... chemical degradation synthetic nucleic acid analogs with similar base pairing properties to DNA may be employed. Examples include peptic nucleic acid, PNA, locked nucleic acid, LNA and 2’ O-methyl modified RNA (Egholm, et al. 1993) (Kaur, et al. 2006) (Sproat, et al. 1989). Functionalization Synthet ...
The amphioxus hairy family: differential fate after duplication.
... the PCR products was used to screen a cDNA library at moderate stringency. This yielded the identification of four different cDNA clones, that we called AmphihairyA, AmphihairyB, AmphihairyC, and AmphihairyD. To study whether more genes of the hairy family were present in amphioxus, we performed a f ...
... the PCR products was used to screen a cDNA library at moderate stringency. This yielded the identification of four different cDNA clones, that we called AmphihairyA, AmphihairyB, AmphihairyC, and AmphihairyD. To study whether more genes of the hairy family were present in amphioxus, we performed a f ...
Genome-Wide Analysis of In Vivo Binding of the Master Regulator
... systems, well represented in the Streptomyces coelicolor genome by 45 ECF (extracytoplasmic function) sigma factors genes and some 70 two-component regulatory systems [4]. As producers of over half of known antibiotics, streptomycetes are a paradigm of secondary metaboliteproducing microorganisms [5 ...
... systems, well represented in the Streptomyces coelicolor genome by 45 ECF (extracytoplasmic function) sigma factors genes and some 70 two-component regulatory systems [4]. As producers of over half of known antibiotics, streptomycetes are a paradigm of secondary metaboliteproducing microorganisms [5 ...
Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter
... each primer, 10% DMSO, and 1 U Taq polymerase (Invitrogen) in a final volume of 50 ll. PCR conditions were 94 C for 2 min, followed by 35 cycles of denaturing at 95 C for 1 min, 58 C for 20 s, and elongation at 72 C for 1 min. Promoter fragments were cloned into a promoter-less luciferase reporte ...
... each primer, 10% DMSO, and 1 U Taq polymerase (Invitrogen) in a final volume of 50 ll. PCR conditions were 94 C for 2 min, followed by 35 cycles of denaturing at 95 C for 1 min, 58 C for 20 s, and elongation at 72 C for 1 min. Promoter fragments were cloned into a promoter-less luciferase reporte ...
Analysis and nucleotide sequence of an origin of DNA replication in
... containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly different from the reported protocol (Singer et al ...
... containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly different from the reported protocol (Singer et al ...
two loci affecting b cell responses to b cell
... the injection of 1.5 ml of FBS into the peritoneum. Macrophages were obtained by incubating 2 x l0 S PEC for 2 h at 37°C on a 15-ram glass coverslip in 1 ml of RPMI1640 medium supplemented with 10% FBS, 10 mM Hepes, 2 mM L-glutamine, and 100 U/ml penicillin/streptomycin, after which the nonadherent ...
... the injection of 1.5 ml of FBS into the peritoneum. Macrophages were obtained by incubating 2 x l0 S PEC for 2 h at 37°C on a 15-ram glass coverslip in 1 ml of RPMI1640 medium supplemented with 10% FBS, 10 mM Hepes, 2 mM L-glutamine, and 100 U/ml penicillin/streptomycin, after which the nonadherent ...
Determining presence/absence threshold for your dataset
... corresponding array name used for this strain was IL1403. So opening that figure would show a histogram as in Fig. 13. It shows the distribution of presence scores of OGs. Using the genome annotation the presence / absence of genes in L. lactis IL1403 is known. Therefore, OGs are divided into 2 grou ...
... corresponding array name used for this strain was IL1403. So opening that figure would show a histogram as in Fig. 13. It shows the distribution of presence scores of OGs. Using the genome annotation the presence / absence of genes in L. lactis IL1403 is known. Therefore, OGs are divided into 2 grou ...
Tombola, a tesmin/TSO1-family protein, regulates
... Jianqiao Jiang*, Elizabeth Benson*, Nina Bausek, Karen Doggett and Helen White-Cooper† During male gametogenesis, a developmentally regulated and cell type-specific transcriptional programme is activated in primary spermatocytes to prepare for differentiation of sperm. The Drosophila aly-class meiot ...
... Jianqiao Jiang*, Elizabeth Benson*, Nina Bausek, Karen Doggett and Helen White-Cooper† During male gametogenesis, a developmentally regulated and cell type-specific transcriptional programme is activated in primary spermatocytes to prepare for differentiation of sperm. The Drosophila aly-class meiot ...
5 CHAPTER 2: LITERATURE REVIEW 2.1 Types of Ribonucleic
... translation without producing any gene product (Kloosterman et al., 2006; Gu et al., 2007; Lindow and Gorodkin, 2007). Humphreys et al. (2005) found that CXCR4 miRNA repressed the translation initiation of Renilla-lucifrase mRNA (contained 4 imperfect binding sites for CXCR4 miRNA at 3’ UTR) by inhi ...
... translation without producing any gene product (Kloosterman et al., 2006; Gu et al., 2007; Lindow and Gorodkin, 2007). Humphreys et al. (2005) found that CXCR4 miRNA repressed the translation initiation of Renilla-lucifrase mRNA (contained 4 imperfect binding sites for CXCR4 miRNA at 3’ UTR) by inhi ...
Down - 서울대 : Biointelligence lab
... “When an axon of a cell A is near enough to excite cell B or repeatedly or persistently takes part in firing it, some growth or metabolic change takes place in both cells such that A’s efficiency, as one of the cells firing B, is increased.” Brain mechanisms and how they can be related to behavi ...
... “When an axon of a cell A is near enough to excite cell B or repeatedly or persistently takes part in firing it, some growth or metabolic change takes place in both cells such that A’s efficiency, as one of the cells firing B, is increased.” Brain mechanisms and how they can be related to behavi ...
PDF - Journal of Neuroscience
... Construction of lentivirus vectors. The sh␣-syn (GAC TTT CAA AGG CCA AGG A) was cloned into the pSI-H1-copGFP vector (SBI Vector) containing the H1 promoter. The control shRNA lentivector (LV-shLuc) contains an shRNA directed against firefly luciferase (SBI Vector). The lentivirus plasmid-expressing ...
... Construction of lentivirus vectors. The sh␣-syn (GAC TTT CAA AGG CCA AGG A) was cloned into the pSI-H1-copGFP vector (SBI Vector) containing the H1 promoter. The control shRNA lentivector (LV-shLuc) contains an shRNA directed against firefly luciferase (SBI Vector). The lentivirus plasmid-expressing ...
Activation of Silent Genes by Transposons Tn5 and TnlO
... the insertion appears to provide a promoter for the expressed gene, but the general phenomenon is difficult to interpret, since not all insertions of a given element serve to activate distal genes (SAEDLER et al. 1974). As exemplified by IS2, several factors complicate the situation. Rearrangementsw ...
... the insertion appears to provide a promoter for the expressed gene, but the general phenomenon is difficult to interpret, since not all insertions of a given element serve to activate distal genes (SAEDLER et al. 1974). As exemplified by IS2, several factors complicate the situation. Rearrangementsw ...
A catalogue of imprinted genes and parent-of
... child with UPD may occur as a result of a population of ill-functioning trisomic cells in the placenta, rather than as a result of altered dose of an imprinted gene(s). For more detailed reviews of UPD, and for justification of conclusions regarding the likelihood of imprinting, see refs 8–11. In mi ...
... child with UPD may occur as a result of a population of ill-functioning trisomic cells in the placenta, rather than as a result of altered dose of an imprinted gene(s). For more detailed reviews of UPD, and for justification of conclusions regarding the likelihood of imprinting, see refs 8–11. In mi ...
Targeted gene expression as a means of altering cell fates and
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
RNA Polymerases
... Transcription factors After RNA polymerase binding, three other transcription factors, TFIIE, TFIIH, and TFIIJ, rapidly asociate with the compex Activation of TFIIH results in phophorylation of the carboxyl-terminal domain (CTD) of the RNA polymerase. This phosphorylation results on formation of a ...
... Transcription factors After RNA polymerase binding, three other transcription factors, TFIIE, TFIIH, and TFIIJ, rapidly asociate with the compex Activation of TFIIH results in phophorylation of the carboxyl-terminal domain (CTD) of the RNA polymerase. This phosphorylation results on formation of a ...
BMC Developmental Biology
... expression of caudal genes, such as Dll1, Notch1, Paraxis and Uncx4.1 [15]. Here, we analysed the requirement of restricted Dll1 expression in epithelial somites for the maintenance of R/ C identity following the initial establishment and for the development of somitocoele cells, in particular. It h ...
... expression of caudal genes, such as Dll1, Notch1, Paraxis and Uncx4.1 [15]. Here, we analysed the requirement of restricted Dll1 expression in epithelial somites for the maintenance of R/ C identity following the initial establishment and for the development of somitocoele cells, in particular. It h ...
Chapter 13 Stress and Glucocorticoid Contributions to Normal and
... [56] for a review). Stressors given immediately prior to assessment of learning and memory may similarly impair [57, 58] or facilitate [59] memory. The effects of repeated stress or GC exposure appear more uniform. Chronic stress consistently impairs measures of brain function. For example, chronic ...
... [56] for a review). Stressors given immediately prior to assessment of learning and memory may similarly impair [57, 58] or facilitate [59] memory. The effects of repeated stress or GC exposure appear more uniform. Chronic stress consistently impairs measures of brain function. For example, chronic ...
PDF
... To acquire accurate data regarding transcriptional regulation, it is preferable to study a var promoter within its chromosomal context that can be both transcriptionally silenced and activated. For this purpose we utilized constructs designed to integrate directly into a var locus in such a way as t ...
... To acquire accurate data regarding transcriptional regulation, it is preferable to study a var promoter within its chromosomal context that can be both transcriptionally silenced and activated. For this purpose we utilized constructs designed to integrate directly into a var locus in such a way as t ...
REVIEW ARTICLE Gene cassettes
... fication and the cassettes are identified by these names (see Hall e t al., 1991). Table 1 lists the boundaries of the cassettes, many of which were not identified or were incorrectly identified in the primary publication of the sequence. The extent of each gene, assuming that the first in-frame ini ...
... fication and the cassettes are identified by these names (see Hall e t al., 1991). Table 1 lists the boundaries of the cassettes, many of which were not identified or were incorrectly identified in the primary publication of the sequence. The extent of each gene, assuming that the first in-frame ini ...
Construction and Characterization of a Highly Regulable Expression
... A number of different expression vectors have been developed to facilitate the regulated overproduction of proteins in Escherichia coli and related bacteria. Some of the more popular ones include pKK223-3, pKK233-2, pTrc99A, and the pET family of expression vectors. These vectors were designed to be ...
... A number of different expression vectors have been developed to facilitate the regulated overproduction of proteins in Escherichia coli and related bacteria. Some of the more popular ones include pKK223-3, pKK233-2, pTrc99A, and the pET family of expression vectors. These vectors were designed to be ...
GENETICS accepted
... unique PCR fragments for the upper and lower strands. These were then transcribed according to the manufacturer’s instructions (Ambion/Applied Biosystems, Austin, TX) and annealed by incubating for 10 minutes at 70°C and slow cooling to room temperature. dsRNA was injected at a total RNA concentrati ...
... unique PCR fragments for the upper and lower strands. These were then transcribed according to the manufacturer’s instructions (Ambion/Applied Biosystems, Austin, TX) and annealed by incubating for 10 minutes at 70°C and slow cooling to room temperature. dsRNA was injected at a total RNA concentrati ...
Biological interpretation of genome-wide association studies using
... cell type context(s) in which the genes’ actions affect phenotypes. Although cell-type-specific expression quantitative trait loci (eQTLs) or coding (non-synonymous) variants in strong LD with associated variants can potentially link these variants to genes, overlap with eQTLs or coding variants may ...
... cell type context(s) in which the genes’ actions affect phenotypes. Although cell-type-specific expression quantitative trait loci (eQTLs) or coding (non-synonymous) variants in strong LD with associated variants can potentially link these variants to genes, overlap with eQTLs or coding variants may ...
Modeling Fragile X Syndrome Using Human Pluripotent Stem Cells
... self-renewal and the ability to differentiate into a wide range of cell types [26]. They are established from in vitro fertilization (IVF) embryos, and can be obtained from genetically affected embryos following preimplantation genetic diagnostic procedures [27]. The derivation of hESCs directly fro ...
... self-renewal and the ability to differentiate into a wide range of cell types [26]. They are established from in vitro fertilization (IVF) embryos, and can be obtained from genetically affected embryos following preimplantation genetic diagnostic procedures [27]. The derivation of hESCs directly fro ...