History of Discoveries in Molecular Biology
... The idea was not the product of a painstaking laboratory discipline, but was conceived while cruising in a Honda Civic on Highway 128 from San Francisco to Mendocino. "I do my best thinking while driving," the scientist with the tanned face and bleached hair once explained. For this brilliant idea b ...
... The idea was not the product of a painstaking laboratory discipline, but was conceived while cruising in a Honda Civic on Highway 128 from San Francisco to Mendocino. "I do my best thinking while driving," the scientist with the tanned face and bleached hair once explained. For this brilliant idea b ...
Identification of One BOCR Mutation and Five NF1 Mutations in Male
... expressed BCL-6 corepressor [19]. It may specifically inhibit the gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least partially by class I and II histone deacetylases [20]. Furthermore, BCL-6 co ...
... expressed BCL-6 corepressor [19]. It may specifically inhibit the gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least partially by class I and II histone deacetylases [20]. Furthermore, BCL-6 co ...
Exam Questions from Exam 1 – Basic Genetic Tests
... Mice homozygous for alleles that block the production of enzyme A (genotype a/a) do not make either antigen 1 or antigen 2. Mice homozygous for defects in the gene encoding enzyme B (genotype b/b) do not make antigen 1. Mice homozygous for defects in the gene encoding enzyme C (genotype c/c) do not ...
... Mice homozygous for alleles that block the production of enzyme A (genotype a/a) do not make either antigen 1 or antigen 2. Mice homozygous for defects in the gene encoding enzyme B (genotype b/b) do not make antigen 1. Mice homozygous for defects in the gene encoding enzyme C (genotype c/c) do not ...
Amino acid alterations essential for increasing the catalytic activity of
... the 140 base substitutions should have been accumulated in the legend to Fig. 2. The activities were expressed as percentages of the 1176-bp of the coding regions [5]. However, only two base the activities/amount of antigenicity towards that of E. coli (pHK4); substitutions finally affected their fu ...
... the 140 base substitutions should have been accumulated in the legend to Fig. 2. The activities were expressed as percentages of the 1176-bp of the coding regions [5]. However, only two base the activities/amount of antigenicity towards that of E. coli (pHK4); substitutions finally affected their fu ...
bantam Encodes a Developmentally Regulated microRNA that
... complex from longer precursor RNAs that form imperfect hairpin structures (Bernstein et al., 2001; Hutvagner et al., 2001). Typically only one arm of the hairpin is recovered as a mature product. Processing of miRNAs has much in common with the production of the short interfering RNAs that direct RN ...
... complex from longer precursor RNAs that form imperfect hairpin structures (Bernstein et al., 2001; Hutvagner et al., 2001). Typically only one arm of the hairpin is recovered as a mature product. Processing of miRNAs has much in common with the production of the short interfering RNAs that direct RN ...
MSc Seminar: Donald Dunbar
... The gene might not be on the chip Can’t differentiate splice variants well The gene might be below detection limit Can’t differentiate RNA synthesis and degradation Can’t tell us about post translational events Bioinformatics can be difficult Relatively expensive February 26th 2011 ...
... The gene might not be on the chip Can’t differentiate splice variants well The gene might be below detection limit Can’t differentiate RNA synthesis and degradation Can’t tell us about post translational events Bioinformatics can be difficult Relatively expensive February 26th 2011 ...
Contrasting Properties of Gene-Specific Regulatory, Coding, and
... Genetic dissection of phenotypic differences within and between species has shown that mutations affecting either the expression or function of a gene product can contribute to phenotypic evolution; mutations that alter gene copy number have also been shown to be an important source of phenotypic va ...
... Genetic dissection of phenotypic differences within and between species has shown that mutations affecting either the expression or function of a gene product can contribute to phenotypic evolution; mutations that alter gene copy number have also been shown to be an important source of phenotypic va ...
Role of 1-aminocyclopropane-1-carboxylate (ACC) Synthases
... that expression levels of RhETR1 were distinctly higher in ‘Bronze’, suggesting that modulation of receptor levels may contribute to ethylene perception and sensitivity. Thus, short flower life in ‘Bronze’ expressing high levels of RhETR1 transcript may contribute to sensitivity to ethylene due to a ...
... that expression levels of RhETR1 were distinctly higher in ‘Bronze’, suggesting that modulation of receptor levels may contribute to ethylene perception and sensitivity. Thus, short flower life in ‘Bronze’ expressing high levels of RhETR1 transcript may contribute to sensitivity to ethylene due to a ...
Supplemental Tables
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
Auxin signals — turning genes on and turning cells around
... PIN protein (red) depends on BFA-sensitive vesicle transport. In BFA-treated cells, PIN accumulates in undefined endosomal membrane structures (light blue) [40,42]. Polar auxin transport depends on an intact actin cytoskeleton, and a high-affinity 1-N-naphthylphthalamic acid (NPA)-binding protein ...
... PIN protein (red) depends on BFA-sensitive vesicle transport. In BFA-treated cells, PIN accumulates in undefined endosomal membrane structures (light blue) [40,42]. Polar auxin transport depends on an intact actin cytoskeleton, and a high-affinity 1-N-naphthylphthalamic acid (NPA)-binding protein ...
Genetics of Male Infertility - the Infertility Center of St. Louis
... sequencing by the usual methods. Therefore, the AZFa section of the Y was initially selected to study in detail, because of the apparent absence of multiple gene copies or Y-specific repeats in that region (104,107). AZFa, has a completely different, more conventional and non-repetitive structure th ...
... sequencing by the usual methods. Therefore, the AZFa section of the Y was initially selected to study in detail, because of the apparent absence of multiple gene copies or Y-specific repeats in that region (104,107). AZFa, has a completely different, more conventional and non-repetitive structure th ...
the genetics of the budgerigar
... the geneticist, not only because it is an excellent material for studies in heredity, now that there are a number of true-breeding varieties, but also because all these are known to have occurred within the last sixty-five years in a species WhlCh has never been crossed with any other. The evolution ...
... the geneticist, not only because it is an excellent material for studies in heredity, now that there are a number of true-breeding varieties, but also because all these are known to have occurred within the last sixty-five years in a species WhlCh has never been crossed with any other. The evolution ...
A gain of function mutation causing skeletal overgrowth in the
... Fig. 2. Cloning of rapunzelc14. (A) A meiotic map representing approximately 47 kb of the rapunzelc14 region on chromosome 16. The critical region contains three paralogous genes (rpz, rpz2 and rpz3). Two additional paralogues (rpz4 and rpz5) are also present on chromosome 16, outside of the critica ...
... Fig. 2. Cloning of rapunzelc14. (A) A meiotic map representing approximately 47 kb of the rapunzelc14 region on chromosome 16. The critical region contains three paralogous genes (rpz, rpz2 and rpz3). Two additional paralogues (rpz4 and rpz5) are also present on chromosome 16, outside of the critica ...
Pultz, M. A., and Baker, B. S.
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
Chromosome Aberrations
... The lesser horseshoe bat (Rhinolophus hipposideros) has 56 chromosomes but the short-tailed fruit bat (Carollia perspicillata) only has 20. Does that mean that the fruit bat is actually an ear of corn or that the horseshoe bat is really a silkworm in disguise? Is one bat “more complex” than the othe ...
... The lesser horseshoe bat (Rhinolophus hipposideros) has 56 chromosomes but the short-tailed fruit bat (Carollia perspicillata) only has 20. Does that mean that the fruit bat is actually an ear of corn or that the horseshoe bat is really a silkworm in disguise? Is one bat “more complex” than the othe ...
The Johns Hopkins University - American University of Beirut
... • Servers have the records you want • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. ...
... • Servers have the records you want • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. ...
Linköping University Post Print Segment-specific Neuronal Sub-type Specification by the Integration of
... ganglion mother cells (GMCs), that in turn typically divide one final time to generate neurons and/or glia [15–18]. Each neuroblast has a unique and stereotypic identity, as revealed by the size of its lineage—ranging from two to 40—and by the types of neurons and glia generated [19–21]. Each thorac ...
... ganglion mother cells (GMCs), that in turn typically divide one final time to generate neurons and/or glia [15–18]. Each neuroblast has a unique and stereotypic identity, as revealed by the size of its lineage—ranging from two to 40—and by the types of neurons and glia generated [19–21]. Each thorac ...
RNA-Seq Alignment v1.0 App Guide
... STAR aligns reads by finding the Maximal Mappable Prefix (MMP) hits between reads (or read pairs) and the genome, using a Suffix Array index. Different parts of a read can be mapped to different genomic positions, corresponding to splicing or RNA-fusions. The genome index includes known splice-junct ...
... STAR aligns reads by finding the Maximal Mappable Prefix (MMP) hits between reads (or read pairs) and the genome, using a Suffix Array index. Different parts of a read can be mapped to different genomic positions, corresponding to splicing or RNA-fusions. The genome index includes known splice-junct ...
NO sensing by FNR: regulation of the Escherichia coli NO
... Anaerobically, Hmp serves as an NO reductase, generating nitrous oxide (Hausladen et al., 1998; Kim et al., 1999). Consistent with the role of Hmp in NO detoxi®cation, the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 199 ...
... Anaerobically, Hmp serves as an NO reductase, generating nitrous oxide (Hausladen et al., 1998; Kim et al., 1999). Consistent with the role of Hmp in NO detoxi®cation, the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 199 ...
Partial report - GEP Community Server
... of updates to the D. melanogaster gene models from FlyBase. many copies as needed within this report. This could result in situations where you will need to construct a new gene model for the coding region prior to performing the TSS annotation. If you find one or more genes with this problem, your ...
... of updates to the D. melanogaster gene models from FlyBase. many copies as needed within this report. This could result in situations where you will need to construct a new gene model for the coding region prior to performing the TSS annotation. If you find one or more genes with this problem, your ...
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference
... 50000 (and the first base of transcript would be 50001 since it is zero-indexed). This would mean that __POINT cycles from 50001-50000=1 to 50000. Note that the difference between 50000 and 1 is 49,999 but the number of bases is 50000 (50000-1+1). The variable P (Line 42) is the absolute position of ...
... 50000 (and the first base of transcript would be 50001 since it is zero-indexed). This would mean that __POINT cycles from 50001-50000=1 to 50000. Note that the difference between 50000 and 1 is 49,999 but the number of bases is 50000 (50000-1+1). The variable P (Line 42) is the absolute position of ...
AmiGO
... which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. ...
... which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. ...
B.Sc. Biomedical Sciences
... (Wherever wet lab experiments are not possible the principles and concepts can be demonstrated through any other material or medium including videos/virtual labs etc.) 1. Preparation of solutions based on molarity, normality, percentage, dilutions etc. 2. Preparation of buffers. 3. Estimation of Moh ...
... (Wherever wet lab experiments are not possible the principles and concepts can be demonstrated through any other material or medium including videos/virtual labs etc.) 1. Preparation of solutions based on molarity, normality, percentage, dilutions etc. 2. Preparation of buffers. 3. Estimation of Moh ...
Algorithms for Finding Gene Clusters
... Definition 1. For a fixed x, a right interval end y > x is called wasteful if it satisfies f (x , y) > 0 for all x ≤ x. Based on this notion, Uno and Yagiura give an algorithm called RC (short for Reduce Candidate) that has as its essential part a data structure Y consisting of a doubly-linked lis ...
... Definition 1. For a fixed x, a right interval end y > x is called wasteful if it satisfies f (x , y) > 0 for all x ≤ x. Based on this notion, Uno and Yagiura give an algorithm called RC (short for Reduce Candidate) that has as its essential part a data structure Y consisting of a doubly-linked lis ...
Discovery and characterization of chromatin states for Please share
... 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% of genome. These states all had in common a high frequency of H3K4me3, but differed in terms of other associated marks, primarily H3K79me2/3, H4K20me1, H3K4me1/2, and H3K9me1, and t ...
... 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% of genome. These states all had in common a high frequency of H3K4me3, but differed in terms of other associated marks, primarily H3K79me2/3, H4K20me1, H3K4me1/2, and H3K9me1, and t ...