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Harvey ras (H-ras) Point Mutations Are Induced by 4
Harvey ras (H-ras) Point Mutations Are Induced by 4

... chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not develop immediately, the acti vated ras oncogene may be detected only when neoplastic develop ment and clonal expansion has ...
Gene Ontology (GO) Tutorial
Gene Ontology (GO) Tutorial

... you will notice that there are two annotations with an “IDA (inferred from direct evidence) code: one says it is in the nucleus; the other says it is not (a different reference). MGI includes both. Occasionally, one will come across gene products that have been annotated to Process, Function, and/or ...
Twinning and Embryonic Left-Right Asymmetry
Twinning and Embryonic Left-Right Asymmetry

... developmental morphology during embryogenesis. The left-right axis of an animal's body plan is often thought of as being fundamentally different from the dorso-ventral and antero-posterior axes because of the symmetry which it exhibits when viewed from the outside. Interestingly though, the internal ...
Loss of heterozygosity at D8S262: an early genetic event of
Loss of heterozygosity at D8S262: an early genetic event of

... precursor lesions that are more advanced than LCC in the course of human hepatocarcinogenesis [4]. Foci of altered hepatocyte (FAH) is observed in several animal species in the early stages of hepatocarcinogenesis caused by chemicals, radiation and chronic infection with hepadnaviruses. FAH is usual ...
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X

... Fluorescence Monitoring in Real-Time When fluorescence signal from a PCR reaction is monitored in real-time, the results can be displayed as an amplification plot (Figure 1), which reflects the change in fluorescence during cycling. This information can be used to quantitate initial copy number bas ...
PDF
PDF

... intron-related CSEs may actually be alternative exons. For example, we found that nine of these ten CSEs have spliced or unspliced human expressed sequence tag (EST) matches. Some of them also shared similarity with mouse mRNAs. There was only one CSE for which we could not get any functional inform ...
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a

... tent cells (including ES cells), either represses Xist transcription, or alternatively reduces levels of a critical positive regulator of Xist expression. Interwoven into this complex regulatory circuitry there is an additional pathway that ensures only a single Xist gene is expressed in XX cells, a ...
The role of Dby mRNA in early development of male mouse zygotes
The role of Dby mRNA in early development of male mouse zygotes

... X-homologue, DBX, located on Xp11, which escapes X-inactivation and has 91.7% sequence identity at the protein level in humans. Slightly different from the human DBY gene, mouse Dby has a structural homolog called Ddx3x on the X chromosome and an autosomal homolog, D1Pas1, on mouse chromosome 1 [8, ...
Regulation of Na/K-ATPase β1 -subunit gene
Regulation of Na/K-ATPase β1 -subunit gene

... Ca2+ and in the force of contraction. This effect on cardiac contractility is the basis of the major therapeutic use of these drugs in the treatment of congestive heart failure. Recently, using cultured neonatal rat cardiac myocytes, we showed that partial inhibition of Na/K-ATPase by ouabain also a ...
Rapsynoid/Partner of Inscuteable Controls Asymmetric Division of
Rapsynoid/Partner of Inscuteable Controls Asymmetric Division of

... Drosophila protein called Raps. We obtained a cDNA (GM02189) containing the complete coding sequence from the Berkeley Drosophila Genome Project EST collection. As shown in Figure 1, A and B, rapsynoid cDNA encodes a 659-amino acid protein that contains seven tetratricopeptide repeats (TPRs), which ...
Origin and Distribution of Calvin Cycle Fructose and
Origin and Distribution of Calvin Cycle Fructose and

... Additional SBP and FBP sequences were retrieved from the public databases through extensive BLAST searches. Raw sequence data (e.g., NCBI Traces) were merged into contigs, and subsequently the deduced aa sequences were used for phylogenetic analyses (see supplementary Table S1). Surprisingly, the gr ...
Full text - PAHdb - McGill University
Full text - PAHdb - McGill University

... first nucleotide of any sequence is numbered from its 50 end. However, to have gDNA nucleotides in register with the older cDNA sequence (which has long served PAH mutation nomenclature), the PAH gDNA has been renumbered in PAHdb: the +1 nucleotide is the adenine of the translation initiation site ( ...
Two distinct tumor suppressor loci within chromosome 11p15
Two distinct tumor suppressor loci within chromosome 11p15

... than previously appreciated (3,4). LOH occurred in at least one marker on the short arm of chromosome 11 in 56 of 94 (60%) informative tumors. The overall frequency of LOH for each marker varies from 16 to 60%, with two peaks seen at markers D11S1318 (45%) and D11S1338 (60%) (Fig. 1). In addition to ...
Protocol: Genome-scale CRISPR-Cas9 Knockout
Protocol: Genome-scale CRISPR-Cas9 Knockout

... the RNA-guided endonuclease Cas9 from the microbial immune system CRISPR (clustered regularly interspaced short palindromic repeat) has proved powerful for precise DNA modifications20-23. Cas9 is guided to specific genomic targets by guide RNAs through Watson-Crick base pairing. As with RNAi, Cas9 i ...
Determining presence/absence threshold for your dataset
Determining presence/absence threshold for your dataset

... corresponding array name used for this strain was IL1403. So opening that figure would show a histogram as in Fig. 13. It shows the distribution of presence scores of OGs. Using the genome annotation the presence / absence of genes in L. lactis IL1403 is known. Therefore, OGs are divided into 2 grou ...
Protocol: Genome-scale CRISPR-Cas9 Knockout
Protocol: Genome-scale CRISPR-Cas9 Knockout

... the RNA-guided endonuclease Cas9 from the microbial immune system CRISPR (clustered regularly interspaced short palindromic repeat) has proved powerful for precise DNA modifications20-23. Cas9 is guided to specific genomic targets by guide RNAs through Watson-Crick base pairing. As with RNAi, Cas9 i ...
The molecular genetics of von Willebrand disease
The molecular genetics of von Willebrand disease

... chromosome 12 VWF gene needs to take into account both the normal vs pseudogene sequences and the frequent polymorphisms present in the VWF gene. The other challenge in interpreting genetic information from the VWF gene is the level of polymorphic variation of the gene sequence. Currently, 81 SNPs h ...
PCB Biodegradation and bphA1 Gene Expression Induced by
PCB Biodegradation and bphA1 Gene Expression Induced by

... isolate P2W. At the same time, the two tested degraders, H850 and P2W, were found to have similar morphology. The growth and PCB degradation capability of two tested bacteria were mediated by amending different organic inducers, including salicylic acid, biphenyl, and salicylic acid plus biphenyl. T ...
Introduction
Introduction

... The section above needs further expansion..needs more on genes possibly involved in sperm-egg interactions eg GEX2 The sperm cells are transported close to the tip of the pollen tube and are, therefore in a prime position to process directional signals for the tube. HAP2 is an integral membrane prot ...
Journal Club 3
Journal Club 3

... Control of the dormancy regulon important for M. tuberculosis survival in latent periods • Dormancy regulon induction inhibits aerobic respiration ...
Multiplex PCR for specific identification and determination of mating
Multiplex PCR for specific identification and determination of mating

... Summary. Togninia minima is one of the fungi involved in esca disease of grapevine, worldwide. It has a biallelic heterothallic mating system. A multiplex PCR test was developed that can detect the species as well as the mating type. A T. minima-specific primer set, with expected amplicon size of 50 ...
FRIZZY PANICLE Drives Supernumerary
FRIZZY PANICLE Drives Supernumerary

... characterization of genetically unrelated mutants leading to the identification of the wheat FRIZZY PANICLE (FZP) gene, encoding a member of the APETALA2/Ethylene Response Factor transcription factor family, which drives the SS trait in bread wheat. Structural and functional characterization of the t ...
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics

... • t(11;11)(q23;q24)/ANLL → MLL - TIRAP • t(11;12)(q23;q13)/ANLL → MLL - CIP29 • t(11;14)(q23;q24)/ANLL, AUL → MLL - GPHN • t(11;15)(q23;q14)/ANLL, ALL → MLL - CASC5 (AF15q14) • t(11;15)(q23;q14) → MLL - MPFYVE • t(11;15)(q23;q15) → MLL - AF15 • t(11;16)(q23;p13)/MDS, ANLL, t-ANLL, ALL → MLL - CREBBP ...
Feline Genetics: a Combinatorial Approach
Feline Genetics: a Combinatorial Approach

... sharply instead of gradually: their effect shows up completely or not at all, according to which variant (allele) of the gene is considered. So, rather than DNA proteins, we shall have the combinatorics of symbols, standing for genes. Please be warned that the mathematical models are aimed to explai ...
- Wiley Online Library
- Wiley Online Library

... its subunits means that failures to resolve individual connections can instead be resolved by disengagement of noncovalent protein subunit interactions, which are presumably far weaker than a chromatid fiber. How does cohesin physically hold sister chromatids together and how does separase so abrupt ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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