GSEA - Bioinformatics Unit
... 1. Choose true (default) to have GSEA collapse each probe set in your expression dataset into a single gene vector, which is identified by its HUGO gene symbol. In this case, you are using HUGO gene symbols for the analysis. The gene sets that you use for the analysis must use HUGO gene symbols to i ...
... 1. Choose true (default) to have GSEA collapse each probe set in your expression dataset into a single gene vector, which is identified by its HUGO gene symbol. In this case, you are using HUGO gene symbols for the analysis. The gene sets that you use for the analysis must use HUGO gene symbols to i ...
Slide 1
... Identify SNPs for gene associated with disease Find the different sets of SNPs in a control population Find different sets of SNPs in affected population Look for unique sets of SNPs in affected population ...
... Identify SNPs for gene associated with disease Find the different sets of SNPs in a control population Find different sets of SNPs in affected population Look for unique sets of SNPs in affected population ...
Lecture 10: Control of gene expression
... concluded that they affect ‘inducibility’ locus. Hence the name lacI. The lacI gene encodes lac repressor that inhibits the lac lacZ- or lacY- mutations cause Lac- phenotype operon. The operon is derepressed by lacI- mutations case Lac++ phenotype lactose in the medium. ...
... concluded that they affect ‘inducibility’ locus. Hence the name lacI. The lacI gene encodes lac repressor that inhibits the lac lacZ- or lacY- mutations cause Lac- phenotype operon. The operon is derepressed by lacI- mutations case Lac++ phenotype lactose in the medium. ...
Presence of Escherichia coli carrying the EAST1 toxin gene in farm
... An EAST1 variant, isolated from EAEC strain O-42, was observed in epidemiological studies [34]. EAST1 O-42 differs from EAST1 17-2 at codon 21 (ACA to GCA) leading to a single amino acid change (T to A) [23]. In humans, this variant is more frequently isolated from diarrhea-associated E. coli strain ...
... An EAST1 variant, isolated from EAEC strain O-42, was observed in epidemiological studies [34]. EAST1 O-42 differs from EAST1 17-2 at codon 21 (ACA to GCA) leading to a single amino acid change (T to A) [23]. In humans, this variant is more frequently isolated from diarrhea-associated E. coli strain ...
Landscape_Evoles Physics
... one base of the genetic code or movement of one atom or amino acid at a time, but rather by exchange of these functional chunks among living organisms ...
... one base of the genetic code or movement of one atom or amino acid at a time, but rather by exchange of these functional chunks among living organisms ...
Arsenite as an Electron Donor for Anoxygenic Photosynthesis
... the oxidation of inorganic electron donors like sulfide [8]. Diverse chemoautotrophic bacteria can oxidize arsenite (As(III)) to arsenate (As(V)) under aerobic or and anaerobic conditions, depending upon the species. The enzymes that support these pathways include a respiratory As(V) reductase (ArrA ...
... the oxidation of inorganic electron donors like sulfide [8]. Diverse chemoautotrophic bacteria can oxidize arsenite (As(III)) to arsenate (As(V)) under aerobic or and anaerobic conditions, depending upon the species. The enzymes that support these pathways include a respiratory As(V) reductase (ArrA ...
Estimates of Selection and Gene Flow From Measures of
... Hybrid zones can yield estimates of natural selection and gene flow. The width of a cline in gene frequency is approximately proportional to gene flow ( u ) divided by the square root of per-locus selection Gene flow also causes gametic correlations (linkage disequilibria) between genes that differ ...
... Hybrid zones can yield estimates of natural selection and gene flow. The width of a cline in gene frequency is approximately proportional to gene flow ( u ) divided by the square root of per-locus selection Gene flow also causes gametic correlations (linkage disequilibria) between genes that differ ...
Directionality in FLP Protein-promoted Site
... metric sequences within the 8-base pair spacer regions. It is were removed at thetimes indicated and treated asdescribed above. possible that the outcome or directionality of recombination The reactions were analyzed by electrophoresis in a 1%horizontal agarose gel. All plasmids used in this work ar ...
... metric sequences within the 8-base pair spacer regions. It is were removed at thetimes indicated and treated asdescribed above. possible that the outcome or directionality of recombination The reactions were analyzed by electrophoresis in a 1%horizontal agarose gel. All plasmids used in this work ar ...
Transcriptome analysis of Drosophila CNS midline cells reveals
... revealed that midline cells express 9 neuropeptide precursor genes, 13 neuropeptide receptor genes, and 31 small-molecule neurotransmitter receptor genes. In situ hybridization and high-resolution confocal analyses were carried-out to determine the midline cell identity for these neuropeptides and t ...
... revealed that midline cells express 9 neuropeptide precursor genes, 13 neuropeptide receptor genes, and 31 small-molecule neurotransmitter receptor genes. In situ hybridization and high-resolution confocal analyses were carried-out to determine the midline cell identity for these neuropeptides and t ...
French 2003 - University of Washington
... shape of the egg. (C) A wild-type stage-12 egg chamber. At this stage, the developing dorsal appendage is apparent as a wedge of cells (bracket) present between the oocyte and nurse cells. Chorion proteins can be seen as a line between the two faces of the wedge. (D) A stage-12 twk egg chamber. Alth ...
... shape of the egg. (C) A wild-type stage-12 egg chamber. At this stage, the developing dorsal appendage is apparent as a wedge of cells (bracket) present between the oocyte and nurse cells. Chorion proteins can be seen as a line between the two faces of the wedge. (D) A stage-12 twk egg chamber. Alth ...
X-adrenoleukodystrophy
... transgenic mice which overexpressed the ALDR gene. The second way was to generate mice that carry an inactivation of the ALDR gene. The authors then performed several experiments on these mice. The first experiment was done to test the generation of the different genotypes of the mice that were used ...
... transgenic mice which overexpressed the ALDR gene. The second way was to generate mice that carry an inactivation of the ALDR gene. The authors then performed several experiments on these mice. The first experiment was done to test the generation of the different genotypes of the mice that were used ...
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene
... forty-two animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.2 gene by means of PCRSSCP. The results identified six genotypes, AA, BB, DD, AB, AD and BD, coded for by three different alleles A, B and D. Two SNPs in the coding region were confirmed by sequen ...
... forty-two animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.2 gene by means of PCRSSCP. The results identified six genotypes, AA, BB, DD, AB, AD and BD, coded for by three different alleles A, B and D. Two SNPs in the coding region were confirmed by sequen ...
Spontaneous Mutations in the CsrRS Two
... its promoter and signal sequence. The hasA gene fragment includes the 235 and 210 sequences that are reportedly protected by CsrR in DNA footprinting experiments [13]. As a negative control, we used a derivative of pABG-5 that contains a rofA-phoZ transcriptional fusion [17] (pABG-5 and the rofA-pho ...
... its promoter and signal sequence. The hasA gene fragment includes the 235 and 210 sequences that are reportedly protected by CsrR in DNA footprinting experiments [13]. As a negative control, we used a derivative of pABG-5 that contains a rofA-phoZ transcriptional fusion [17] (pABG-5 and the rofA-pho ...
Congenital hereditary cataracts
... (opaque flecks in the lens, Opj; Lyon et al., 2003). The point mutation affects the basic region of the DNA-binding domain. In general, Maf binds as homo- or heterodimer to two known Maf responsive elements (MAREs), with varying affinities and transactivation potentials (Kataoka et al., 1993; 1996). ...
... (opaque flecks in the lens, Opj; Lyon et al., 2003). The point mutation affects the basic region of the DNA-binding domain. In general, Maf binds as homo- or heterodimer to two known Maf responsive elements (MAREs), with varying affinities and transactivation potentials (Kataoka et al., 1993; 1996). ...
Identification and isolation of active N2O reducers in rice paddy soil
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
Biophysics of Macromolecules: Problem set 4
... a) The minimum and maximum RMS values are 1.122112 nm and 5.755623 nm, respectively. b) Single layer origami structures are based on parallel DNA double helices that are connected to each other by inter-strand crossovers every 32 bp. As discussed in class, this value was chosen to be approximately c ...
... a) The minimum and maximum RMS values are 1.122112 nm and 5.755623 nm, respectively. b) Single layer origami structures are based on parallel DNA double helices that are connected to each other by inter-strand crossovers every 32 bp. As discussed in class, this value was chosen to be approximately c ...
PHI-Canto video tutorial text - PHI-base
... And we move onto Step5c adding an annotation term. # In this case to the curation type ‘PHI phenotype’. # I am looking for ‘unaffected pathogenicity’ in the search box # which is one of 9 upper level PHI phenotype outcome terms used in the preliminary annotation. Subsequent additional PHI phenotype ...
... And we move onto Step5c adding an annotation term. # In this case to the curation type ‘PHI phenotype’. # I am looking for ‘unaffected pathogenicity’ in the search box # which is one of 9 upper level PHI phenotype outcome terms used in the preliminary annotation. Subsequent additional PHI phenotype ...
Complete comparative genomic analysis of two field isolates of
... variable region is located between hr1 and orf31 (bro-c) (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes ...
... variable region is located between hr1 and orf31 (bro-c) (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes ...
Homo-Dimeric Spherulin 3a: A Single
... presence of DTT as a reducing agent clearly proves this interpretation to be valid. To ensure a well-defined state of association of the native protein and to avoid side reactions of the cysteine residues (e.g., aggregation of the thermally denaturated protein), the cysteine in position 4 was replac ...
... presence of DTT as a reducing agent clearly proves this interpretation to be valid. To ensure a well-defined state of association of the native protein and to avoid side reactions of the cysteine residues (e.g., aggregation of the thermally denaturated protein), the cysteine in position 4 was replac ...
Recombination and the Frequency Spectrum in
... tree. However, recombination causes different sites to have trees that differ from each other. These trees are not independent of each other, since they share parts of their genealogical histories. For a more detailed explanation, see Hudson (1990). Estimating Parameters For the population recombina ...
... tree. However, recombination causes different sites to have trees that differ from each other. These trees are not independent of each other, since they share parts of their genealogical histories. For a more detailed explanation, see Hudson (1990). Estimating Parameters For the population recombina ...
The Arabidopsis Protein SHI Represses
... but are generally poorly characterized at the genetic and molecular level. One exception is the response to GA in germinating cereal grains. The key response is the production of the hydrolytic enzyme ␣-amylase, which is synthesized in the aleurone cells during germination for breakdown and mobiliza ...
... but are generally poorly characterized at the genetic and molecular level. One exception is the response to GA in germinating cereal grains. The key response is the production of the hydrolytic enzyme ␣-amylase, which is synthesized in the aleurone cells during germination for breakdown and mobiliza ...
Global Transcriptional Profiling Reveals Distinct Functions of Thymic
... Functions of Thymic Stromal Subsets We identified differentially expressed genes (DEGs) in each stromal subset as those genes uniquely up- or downregulated by >2fold relative to all other stromal subsets of the same age, with an adjusted p value < 0.01 (Table S1). As expected from PCA analysis, mTEC ...
... Functions of Thymic Stromal Subsets We identified differentially expressed genes (DEGs) in each stromal subset as those genes uniquely up- or downregulated by >2fold relative to all other stromal subsets of the same age, with an adjusted p value < 0.01 (Table S1). As expected from PCA analysis, mTEC ...
PDF 743ko
... Maternal determinants: Maternal determinants are factors (mRNAs, proteins or their complexes) inherited from the oocyte which direct axis formation and/or the differentiation of tissues in the embryo. When maternal determinants are inhibited or removed, axis formation or development are abnormal. Wh ...
... Maternal determinants: Maternal determinants are factors (mRNAs, proteins or their complexes) inherited from the oocyte which direct axis formation and/or the differentiation of tissues in the embryo. When maternal determinants are inhibited or removed, axis formation or development are abnormal. Wh ...
2. Assessing the probative value of DNA evidence
... We recognise, nonetheless, that certain parts of the following discussion may be of greater interest and practical utility to some criminal justice professionals than to others. Our hope is that judges, lawyers and forensic scientists will be able to extrapolate from the common core and adapt our ge ...
... We recognise, nonetheless, that certain parts of the following discussion may be of greater interest and practical utility to some criminal justice professionals than to others. Our hope is that judges, lawyers and forensic scientists will be able to extrapolate from the common core and adapt our ge ...
Population genetics by Knud Christensen
... is influenced by a lot of minor environmental effects. Diseases will often be 'either/or traits' as the simple Mendelian traits. Cases in which the severity of the disease has a normal distribution can also be found. In many production diseases the disease only occurs when a genetically prone indivi ...
... is influenced by a lot of minor environmental effects. Diseases will often be 'either/or traits' as the simple Mendelian traits. Cases in which the severity of the disease has a normal distribution can also be found. In many production diseases the disease only occurs when a genetically prone indivi ...