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View PDF - BloodMed
View PDF - BloodMed

... appeared to be alleles of Hb S, in 1958 Smith and Torbert, research fellows working at Johns Hopkins Hospital in Lockard Conley's Department, discovered a family in which two haemoglobin variants, Hbs Hopkins 2 and S, segregated independently. Shortly afterwards, in a series of ingenious dissociatio ...
Epigenetic inheritance of acquired traits through sperm RNAs and
Epigenetic inheritance of acquired traits through sperm RNAs and

... (tsRNAGly(GCC))11 could change early embryonic gene expression, indicating that sperm tsRNAs play an active part in the transmission of paternally acquired traits by reshaping early embryonic development. A recent study revealed that sperm tsRNAs show a dramatic increase in abundance during late spe ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and

... 16SrX group (Lee et al., 2000) within the AP subclade, which is one of the major branches of the phytoplasma clade. The four phytoplasmas differ from each other in <2?5 % of 16S rDNA nucleotide sequence positions. Thus, the objective of this work was to examine whether the above-mentioned requiremen ...
Variable Expression of the Mutation in Familial Defective
Variable Expression of the Mutation in Familial Defective

... inherited a rare mutant non-FDB allele at the apoB locus, whose product was underrepresented in his plasma. In support of this, they showed that the IQo of J.H.'s LDL (determined by a competitive binding assay) was two to three times the IQoS of the LDLs obtained from his heterozygous relatives, and ...
GO and Annotation
GO and Annotation

... GO tools • Most of these tools work in a similar way: – input a gene list and a subset of ‘interesting’ genes – tool shows which GO categories have most interesting genes associated with them i.e. which categories are ‘enriched’ for interesting genes – tool provides a statistical measure to determi ...
Application of whole genome sequencing to fully characterise
Application of whole genome sequencing to fully characterise

... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
Small Nucleolar RNA
Small Nucleolar RNA

... snoRNAs can be used as a biological tool • (1) snoRNA localization elements have been utilized to direct new RNA sequences to the nucleolus • (2) snoRNAs have been used to direct nucleotide modifications to new sites in rRNA via the expression of snoRNAs containing novel guide sequences ...
Harrisslides
Harrisslides

... aspects of molecular biology • Describe gene products using vocabulary terms (annotation) • Develop tools: • to query and modify the vocabularies and annotations • annotation tools for curators ...
Developing expressed sequence tag libraries and
Developing expressed sequence tag libraries and

... amplifying a product of which 17 (71 %) detected polymorphisms in R. idaeus. These results indicate that markers that amplify a polymorphic product in highlyhomozygous black raspberry are likely to amplify a polymorphic product in red raspberry, regardless of the sequence source. ...
Molecular mechanics of the interactions of spermine with DNA: DNA
Molecular mechanics of the interactions of spermine with DNA: DNA

... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
Bacteriophage MS2 RNA
Bacteriophage MS2 RNA

... Finally, one important question is to know if our observation made with Bacteriophase MS2 RNA can be generalized to other prokaryotic mRNA. Unfortunately, at present it is not possible to compare the results with enough long sequences of prokaryotic mRNAs. However, a comparison can be made with the ...
scope and use of bioinformatics in genomic and proteomic
scope and use of bioinformatics in genomic and proteomic

... services to the Swiss biomedical research community within the framework of EMBnet and NCCR Together with the Universities of Lausanne, Geneva and Basel, the Swiss Federal Institutes of Technology of Lausanne (EPFL) and Zurich (EPFZ), and three private partners, Hewlett-Packard Inc., Intel Corp. and ...
Deletions of NF1 gene and exons detected by multiplex ligation
Deletions of NF1 gene and exons detected by multiplex ligation

... Usually, NF1 diagnostic screening strategies employ PCRbased screening methods such as the protein truncation test (PTT),17 25 single-strand conformational polymorphism (SSCP),26 or denaturing high-performance liquid chromatography (DHPLC)15 27 with varying degrees of sensitivity for each method. Di ...
4 Mapping Eukaryotic Chromosomes by
4 Mapping Eukaryotic Chromosomes by

... crossovers at various locations along the chromosome. There are five total crossovers in the A-C region of this chromosome. One crossover occurs in the A-B region, for a frequency of 0.20. Four crossovers (two singles and a threestranded double) occur in the B-C region for a frequency of 0.80. Becau ...
Macaya Whole STUDENT`S WORKSHEETS
Macaya Whole STUDENT`S WORKSHEETS

... Haemophilia is a sex-linked disorder. Its gene is in chromosome sexual X. Since males (XY) have only one X chromosome, so only one copy of the gene placed in this chromosome, if the gene is they will be affected with the disease. Females have two X chromosome (XX) only will have the disease when the ...
Reciprocal deletion and duplication at 2q23.1 indicates a
Reciprocal deletion and duplication at 2q23.1 indicates a

... eyebrows, prominent nose, small chin, thin upper lip), and minor digital anomalies (fifth finger clinodactyly and large broad first toe). The microduplication size varies among all cases and ranges from 68 kb to 53.7 Mb, encompassing a region that includes MBD5, an important factor in methylation pa ...
Concordance trees, concordance factors, and the exploration of
Concordance trees, concordance factors, and the exploration of

... to say, for all times in the past, one knew the assignment until either a fully resolved tree has been obtained or all of individuals to interbreeding populations, the descent relations of those populations, and the extent of gene flow compatible clades have been added to the tree. It is worth betwe ...
Conservation and diversification of gene function during mouthpart
Conservation and diversification of gene function during mouthpart

... The mandibles are the chewing mouthparts, articulated to the head by two condyles. From the base of the mandibles extend two lobes: the distal incisor lobe and the inner median molar lobe, which are linked by a flexible area (Fig. 5Ai to iv). The setae on the inner periphery of the incisor lobe delim ...
Conserved syntenic clusters of protein coding genes are missing in
Conserved syntenic clusters of protein coding genes are missing in

... putative missing genes in birds cluster into approximately 100 conserved syntenic blocks in lizard and humans. The approximately 1,000 remaining candidate missing genes occur as singletons throughout non-avian genomes, or are associated with segments that have not been included in the main avian ass ...
Fkh5-deficient mice show dysgenesis in the caudal midbrain and
Fkh5-deficient mice show dysgenesis in the caudal midbrain and

Conserved syntenic clusters of protein coding genes are missing in birds
Conserved syntenic clusters of protein coding genes are missing in birds

... putative missing genes in birds cluster into approximately 100 conserved syntenic blocks in lizard and humans. The approximately 1,000 remaining candidate missing genes occur as singletons throughout non-avian genomes, or are associated with segments that have not been included in the main avian ass ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
29th International Conference on Animal Genetics ISAG2004/TOKYO

... 8. Rabbit Gene Mapping (Chair: Hein A. Van Lith) September 11 (Saturday) 16:00-18:00 at Room 309 G (Academy common 9F) 9. Gene Networks for Beef Marbling (Chair: Kenji Oyama) September 11 (Saturday) 16:00-18:00 at Room 309 F (Academy common 9F) TG5 and other genes for beef marbling William Barendse ...
Chapter 4 - DORAS
Chapter 4 - DORAS

... obtained from the in silico analysis. This was achieved by insertional inactivation of the genes using antibiotic resistance cassettes designed to exert a polar effect on the downstream genes in the operon. The mutants were constructed in a 2011rhtX3hmuT::Km background in order to facilitate observa ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
Harvey ras (H-ras) Point Mutations Are Induced by 4

... chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not develop immediately, the acti vated ras oncogene may be detected only when neoplastic develop ment and clonal expansion has ...
Identifying Common Genetic Variants by High
Identifying Common Genetic Variants by High

... detected variants (7, 8, 11, 13 ). Indeed, heteroduplex scanning methods as a rule detect sequence variants but do not identify or genotype those variants. Common sequence variants that do not cause disease occur at a frequency much greater than that of disease-causing variants. These common variant ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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