A TaqI polymorphism in the 3 UTR of the IL-12
... level of conservation in humans. Several intronic polymorphisms however were identified, as well as a TaqI RFLP at position 1188 in the 30 UTR.14 The latter is of particular interest, since it has been shown to be associated with susceptibility to at least two diseases: multiple sclerosis17 and type ...
... level of conservation in humans. Several intronic polymorphisms however were identified, as well as a TaqI RFLP at position 1188 in the 30 UTR.14 The latter is of particular interest, since it has been shown to be associated with susceptibility to at least two diseases: multiple sclerosis17 and type ...
Bcl-2–Modifying Factor Induces Renal Proximal Tubular
... Validation of Bmf mRNA expression in mouse RPTs by real-time PCR. To validate the results obtained by DNA microarray, real-time qPCR was performed using primers specific for mouse Bmf mRNA. Figure 1A displays the results of Bmf mRNA expression in freshly isolated RPTs from db/m+, db/db, and db/db CAT ...
... Validation of Bmf mRNA expression in mouse RPTs by real-time PCR. To validate the results obtained by DNA microarray, real-time qPCR was performed using primers specific for mouse Bmf mRNA. Figure 1A displays the results of Bmf mRNA expression in freshly isolated RPTs from db/m+, db/db, and db/db CAT ...
Molecular Biology Reports
... cyclases, lycopene e-cyclase (LCY-e) and lycopene betacyclase (LCY-b). LCY-e introduces a single epsilon-ring into lycopene to produce d-carotene, and LCY-b introduces one or two beta-rings at either end of lycopene to produce b-carotene. Two carotenoid biosynthetic pathways proceed from lycopene, t ...
... cyclases, lycopene e-cyclase (LCY-e) and lycopene betacyclase (LCY-b). LCY-e introduces a single epsilon-ring into lycopene to produce d-carotene, and LCY-b introduces one or two beta-rings at either end of lycopene to produce b-carotene. Two carotenoid biosynthetic pathways proceed from lycopene, t ...
Restless Legs Syndrome
... • Disease nosologies are human constructs informed by physician experience, clinical expediency, and regulatory agencies, but are most often lack a complete understanding of the biology of the disease. • The RLS story provides the best example to date of how discovery of sequence variants can influ ...
... • Disease nosologies are human constructs informed by physician experience, clinical expediency, and regulatory agencies, but are most often lack a complete understanding of the biology of the disease. • The RLS story provides the best example to date of how discovery of sequence variants can influ ...
Inference of homologous recombination in bacteria using whole
... organisms, while simultaneously identifying for each branch of that genealogy the genomic locations where recombination occurred. The implementation of that method in software is called ClonalFrame [13]. ClonalFrame has proved useful to identify interesting patterns of recombination in a wide variet ...
... organisms, while simultaneously identifying for each branch of that genealogy the genomic locations where recombination occurred. The implementation of that method in software is called ClonalFrame [13]. ClonalFrame has proved useful to identify interesting patterns of recombination in a wide variet ...
Ribosomal proteins promote leaf adaxial identity
... required for the normal leaf polarity (Huang et al., 2006). Here, we implicate a new class of genes, the ribosomal large subunit protein encoding genes, in leaf polarity establishment. In animals, functions of the ribosomal proteins have been investigated extensively, and defects in these proteins c ...
... required for the normal leaf polarity (Huang et al., 2006). Here, we implicate a new class of genes, the ribosomal large subunit protein encoding genes, in leaf polarity establishment. In animals, functions of the ribosomal proteins have been investigated extensively, and defects in these proteins c ...
Pathway Analysis using Partek Genomics Suite® 6.6 and Partek
... If you use Pathway on the selected species for the first time, Pathway will automatically download the Kegg information needed for the analysis. Once the pathway enrichment calculation has been performed a new spreadsheet is created (Pathway-Enrichment.txt) and Partek Pathway will be launched (Figur ...
... If you use Pathway on the selected species for the first time, Pathway will automatically download the Kegg information needed for the analysis. Once the pathway enrichment calculation has been performed a new spreadsheet is created (Pathway-Enrichment.txt) and Partek Pathway will be launched (Figur ...
Rather than test an unmapped gene successively for linkage to... groups, it is advantageous to test all linkage groups in... alcoy David Perkins
... FIGURE 1. Linkage relations in a cross of the linkage tester alcoy; csp-2 × Normal sequence. Chromosomes are shown paired as in meiotic prophase I. The alcoy; csp-2 chromosomes are drawn as heavy lines, and those from the standard-sequence parent are shown as thin lines. Segments from odd-numbered l ...
... FIGURE 1. Linkage relations in a cross of the linkage tester alcoy; csp-2 × Normal sequence. Chromosomes are shown paired as in meiotic prophase I. The alcoy; csp-2 chromosomes are drawn as heavy lines, and those from the standard-sequence parent are shown as thin lines. Segments from odd-numbered l ...
Genetic diversity and epidemiology of infectious hematopoietic
... region was selected for sequencing since it contains a for each probe were reproducible for repeated RPA reactions of the same isolate. The sizes of the haploputative antigenic determinant and is a region of high type groups, defined as the number of isolates represequence diversity (Nichol et al. 1 ...
... region was selected for sequencing since it contains a for each probe were reproducible for repeated RPA reactions of the same isolate. The sizes of the haploputative antigenic determinant and is a region of high type groups, defined as the number of isolates represequence diversity (Nichol et al. 1 ...
Telomere maintenance without telomerase
... consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also displayed dramatic changes at their chromosomal termini, due to global ampli®cation of both telomeric and sub-telomeric repeat sequences. These extensive rearrangeme ...
... consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also displayed dramatic changes at their chromosomal termini, due to global ampli®cation of both telomeric and sub-telomeric repeat sequences. These extensive rearrangeme ...
Introduction to Genetics
... - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small amount of protein. The same happens in cellular organelles such as mitochondria . - In eukaryotes, since the amount of DNA from each chromosome is very large, the packing must be more complex ...
... - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small amount of protein. The same happens in cellular organelles such as mitochondria . - In eukaryotes, since the amount of DNA from each chromosome is very large, the packing must be more complex ...
Transvection Is Common Throughout the Drosophila
... gen) of the vector used to introduce the CRM-GAL4. A schematic of an integrated CRM-GAL4 is shown in Figure 1A. While screening through CRM-GAL4s that had been integrated into attP2, we identified two transgenes, R24B02GAL4 and R58G08-GAL4, that drove expression in neuroblast hemilineages 12A and 3A ...
... gen) of the vector used to introduce the CRM-GAL4. A schematic of an integrated CRM-GAL4 is shown in Figure 1A. While screening through CRM-GAL4s that had been integrated into attP2, we identified two transgenes, R24B02GAL4 and R58G08-GAL4, that drove expression in neuroblast hemilineages 12A and 3A ...
Premature stop codons involved in muscular dystrophies
... model closely corresponds to the physiological situation, in which a given mutation is embedded in a unique nucleotide context, and differs between patients. Several reasons indicated an investigation of gentamicin: its effect on readthrough is the best documented to date, and it is known to present ...
... model closely corresponds to the physiological situation, in which a given mutation is embedded in a unique nucleotide context, and differs between patients. Several reasons indicated an investigation of gentamicin: its effect on readthrough is the best documented to date, and it is known to present ...
Tadashi Inagami, Deng-Fu Guo and Yutaka Kitami
... this question, AT 1 receptor from rat adrenal (AT 1B) was cloned [15,16] and was found to be different the receptor_identified in, vascular and renal-tissue The cDNA for AT 1 cloned from rat pituitary was identical with that expressed dominantly in the rat adrenal gland (AT1B) [17]. The presence of ...
... this question, AT 1 receptor from rat adrenal (AT 1B) was cloned [15,16] and was found to be different the receptor_identified in, vascular and renal-tissue The cDNA for AT 1 cloned from rat pituitary was identical with that expressed dominantly in the rat adrenal gland (AT1B) [17]. The presence of ...
Distalless and dachshund pattern both plesiomorphic
... that of the spider. Harvestmen may be considered to represent a lineage of “primitive” arachnids (like scorpions), as they bear a number of plesiomorphic structures not observed in spiders or mites. Their early appearance in the fossil record and placement in most chelicerate phylogenies enables phy ...
... that of the spider. Harvestmen may be considered to represent a lineage of “primitive” arachnids (like scorpions), as they bear a number of plesiomorphic structures not observed in spiders or mites. Their early appearance in the fossil record and placement in most chelicerate phylogenies enables phy ...
Term Identification in the Biomedical Literature*
... To give an example of the term identification steps, consider a hypothetical sentence such as ‘p53 protein suppresses mdm2 expression’ in an article on human signal transduction. We use term recognition to find the term boundaries for the two entities of interest (p53 protein and mdm2). Then, we cat ...
... To give an example of the term identification steps, consider a hypothetical sentence such as ‘p53 protein suppresses mdm2 expression’ in an article on human signal transduction. We use term recognition to find the term boundaries for the two entities of interest (p53 protein and mdm2). Then, we cat ...
Single-Nucleotide Polymorphism Mapping
... SNP, this may be an increase in cut or in uncut DNA. The ratio is the relevant parameter, not the absolute amount of product. When using eight SNPs per chromosome, a mutation will show tight linkage to at least one SNP and noticeable linkage to adjacent SNPs. Weak linkage to one SNP, but not to adja ...
... SNP, this may be an increase in cut or in uncut DNA. The ratio is the relevant parameter, not the absolute amount of product. When using eight SNPs per chromosome, a mutation will show tight linkage to at least one SNP and noticeable linkage to adjacent SNPs. Weak linkage to one SNP, but not to adja ...
Population Differences in the Polyalanine Domain and 6
... likely lead to loss of the signal for initiation of translation, suggesting either that no protein is produced or the translation initiation site moves up- or downstream. In HLXB9, the next in-frame ATG codon would correspond to methionine 223. Translation initiation from this internal ATG site woul ...
... likely lead to loss of the signal for initiation of translation, suggesting either that no protein is produced or the translation initiation site moves up- or downstream. In HLXB9, the next in-frame ATG codon would correspond to methionine 223. Translation initiation from this internal ATG site woul ...
Real-time PCR Handbook
... One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA, allowing the polymerase to synthesize nonspecific product. The problem of nonspecific products resulting from mis-priming can be ...
... One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA, allowing the polymerase to synthesize nonspecific product. The problem of nonspecific products resulting from mis-priming can be ...
1 Article: Investigation Evidence for Stabilizing Selection on Codon
... Standard models of selection on codon usage predict that the intensity of selection scales with Ne (Li 1987; Bulmer 1991).Within a species, Ne can vary within the genome of a species due to differences in recombination rates across chromosomes and the effects of background selection. Regions of the ...
... Standard models of selection on codon usage predict that the intensity of selection scales with Ne (Li 1987; Bulmer 1991).Within a species, Ne can vary within the genome of a species due to differences in recombination rates across chromosomes and the effects of background selection. Regions of the ...
Compound heterozygosity of novel missense
... Mutations associated with hereditary combined VKD coagulation factor deficiency are rare. The carboxylase is encoded by a single gene,29 and only 3 naturally occurring missense mutations have previously been identified: Leu394Arg,10 which is impaired in both Glu binding and propeptide binding,30 Trp ...
... Mutations associated with hereditary combined VKD coagulation factor deficiency are rare. The carboxylase is encoded by a single gene,29 and only 3 naturally occurring missense mutations have previously been identified: Leu394Arg,10 which is impaired in both Glu binding and propeptide binding,30 Trp ...
Understanding the pathological manifestations of aromatase excess
... Recent studies have revealed that promoters of so-called housekeeping genes are recruited for the aberrant expression of aromatase in AEXS [1,7,8]. Thus, aromatase is probably expressed continuously and ubiquitously in all somatic cells, as are housekeeping genes, which is in contrast to the continu ...
... Recent studies have revealed that promoters of so-called housekeeping genes are recruited for the aberrant expression of aromatase in AEXS [1,7,8]. Thus, aromatase is probably expressed continuously and ubiquitously in all somatic cells, as are housekeeping genes, which is in contrast to the continu ...
Method for identification of origins of replication and genes
... methylase sites. Define: DnaA box Dam methylase box Search for DnaA boxes on the genomic sequence, look until found a match. Remember the coordinate of DnaA box as Start of Region. For 500 hp region after the match: search for any additional DnaA boxes in the region search for any Dam binding sites ...
... methylase sites. Define: DnaA box Dam methylase box Search for DnaA boxes on the genomic sequence, look until found a match. Remember the coordinate of DnaA box as Start of Region. For 500 hp region after the match: search for any additional DnaA boxes in the region search for any Dam binding sites ...