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BURN INJURY
BURN INJURY

... High output enteric fistula distal to feeding tube Inability to gain access Intractable vomiting or diarrhea Aggressive therapy not warranted Expected need less than 5-7 days if malnourished or 7-9 days if normally nourished ...
Decarboxylation Reactions Major concepts Decarboxylation
Decarboxylation Reactions Major concepts Decarboxylation

...  When a carboxylate is “Beta” to a carbonyl, spontaneous decarboxylation can result and requires no cofactor.  When a carboxylate is “Alpha” to a carbonyl, decarboxylation requries a cofactor called Thiamine pyrophosphate (TPP) to stabilize the intermediates in the reaction (electron sink.)  When ...
5-MGD Session 3, Lec 5, 2014
5-MGD Session 3, Lec 5, 2014

... Enzyme Commission : established the nomenclature for enzymes • numbers that follow E.C. gives the identity of the enzyme ...
Inborn Errors of Metabolic Etiology
Inborn Errors of Metabolic Etiology

... The ER physician starts an IV and gives a bolus of glucose to correct hypoglycemia. The physician also gives normal saline boluses for rehydration. Then IVFs with D5 ¼ normal saline is started at 1.5 maintenance fluids. Followup labs show normal serum glucose but no change in acid-base status. The p ...
Evolution by leaps: gene duplication in bacteria | SpringerLink
Evolution by leaps: gene duplication in bacteria | SpringerLink

... variation arising from many avenues: single base changes, horizontal transfer of genes, loss of genes, rearrangements of genomic segments and, discussed here, gene duplication followed by divergence of the copies. The comparative analysis of sequences of related and unrelated bacteria has filled out ...
Law (Principle) of Dominance The law (principle) of dominance
Law (Principle) of Dominance The law (principle) of dominance

... The genotype (genetic makeup) of an organism reveals the type of alleles that an organism has inherited for a particular trait. The genotype for a particular trait is usually represented by a letter, the capital letter representing the dominant gene and the lower-case letter representing the recessi ...
II. Beta oxidation of fatty acid
II. Beta oxidation of fatty acid

... IX. Diseases associated with purine and pyrimidine metabolism QUESTIONS ON NUCLEIC ACID CHEMISTRY & METABOLISM _B__56. The process of producing DNA from a molecule of RNA: A. replication C. translation B. Reverse transcription D. conjugation _B__57. This DNA form is seen in physiologic conditions wh ...
Metabolic Fate of Glucose Metabolic Fate of Fatty Acids
Metabolic Fate of Glucose Metabolic Fate of Fatty Acids

... • Dietary fuel from a breakfast is processed slightly differently by the liver than during a continued fed-state. • The liver spares the glucose for peripheral tissues. Instead it continues to synthesize glucose by gluconeogenesis. • Since the blood glucose levels are above normal, the newly synthes ...
Protein Buffer Systems
Protein Buffer Systems

... affinity for both CO2 and H+; thus, its buffering effect is strongest in the tissues. Little CO2 is produced in red cells and so the CO2 produced by the tissues passes easily into the cell down a concentration gradient. Carbon dioxide then either combines directly with haemoglobin or combines with w ...
Practice Exam 3
Practice Exam 3

... Name the two enzymes that catalyze a reaction in which ATP is consumed? __________________________________________ Which enzyme catalyzes a reaction in which NADH is produced? _____________________ Which enzyme converts G3P into 1,3 BPG? __________________________ Name two enzyme reactions from glyc ...
Practice Exam 3 Answers
Practice Exam 3 Answers

... Name the two enzymes that catalyze a reaction in which ATP is consumed? __________________________________________ Which enzyme catalyzes a reaction in which NADH is produced? _____________________ Which enzyme converts G3P into 1,3 BPG? __________________________ Name two enzyme reactions from glyc ...
Chapter 04 - Lecture Outline
Chapter 04 - Lecture Outline

... Name the two major divisions of metabolism, and compare and contrast them in terms of a general descriptive sentence, additional descriptive terms, how energy is involved, whether bonds are formed or broken, and how water is involved. Also write a chemical reaction for each and give an example impor ...
Inborn errors of Metabolism (IEM)
Inborn errors of Metabolism (IEM)

... Investigations likely to be directed by presentation Acute vs Chronic Acute: may be medical emergency, hypoglycaemia, hyperammonaemia, metabolic acidosis Chronic: more difficult, need to decide how many investigations to persue ...
Phenotype Sequencing - Bioinformatics Research Group
Phenotype Sequencing - Bioinformatics Research Group

... Starting with a parent organism, create many mutants using random mutagenesis (e.g. UV, NTG) ...
Single Replacement Reactions - Tri
Single Replacement Reactions - Tri

... • Single Replacement Reactions occur when one element replaces another in a compound. • A metal can replace a metal (+) OR a nonmetal can replace a nonmetal (-). • element + compound compound + element A + BC  AC + B (if A is a metal) OR A + BC  BA + C (if A is a nonmetal) ...
Protein Buffer Systems
Protein Buffer Systems

... affinity for both CO2 and H+; thus, its buffering effect is strongest in the tissues. Little CO2 is produced in red cells and so the CO2 produced by the tissues passes easily into the cell down a concentration gradient. Carbon dioxide then either combines directly with haemoglobin or combines with w ...
Lecture #11 – 9/28 – Dr. Hirsh
Lecture #11 – 9/28 – Dr. Hirsh

enzyme powerpoint
enzyme powerpoint

... What are the RIGHT conditions? 1)Specific temperature ranges. (The enzymes in your body work at what temperature? The enzymes in the bacteria that live in the hot springs work at a much higher temperature). 2) Specific pH ranges (Your stomach enzymes work at what pH? Your mouth?) 3) Specific substr ...
Application of Biological Network
Application of Biological Network

... genes to the hub proteins and the interaction of genes with others. • Fig(a) shows us a tendency of all disease genes to form hubs, where we could see the tendency for disease genes to encode proteins with hubs. • Fig(c) shows the same relationship but with only essential disease genes where the ten ...
Plant Response to Stress: Biochemical Adaptations to
Plant Response to Stress: Biochemical Adaptations to

... are conserved in a wide variety of plants from very different environments. The biochemical adaptations of Pi plants provides an excellent example of how the unique flexibility of plant metabolism and energy transduction helps them cope in a typically stressful environment. These adaptations also pr ...
Final Exam - UC Davis Plant Sciences
Final Exam - UC Davis Plant Sciences

... Graduate Student This exam consists of 8 questions. A maximum of 100 points can be earned. Partial credit will be given. There are a total of 12 pages, including the cover page and one blank sheet at the end for notes. However, do not use the blank sheet for your final answers. If you need more spac ...
Ketoacidosis - Wellington ICU
Ketoacidosis - Wellington ICU

... - ketoacidosis is a high anion gap metabolic acidosis due to an excessive blood concentration of ketone bodies (keto-anions). - ketone bodies (acetoacetate, beta-hydroxybutyrate, acetone) are released into the blood from the liver when hepatic lipid metabolism has changed to a state of increased ket ...
Lect2(Enzim
Lect2(Enzim

... reduction of N2 to ammonia). Nitrogenase catalyses this reaction at temperatures around 300 K and at neutral pH. The enzyme is a complex system comprising two dissociating protein components one of which contains iron and the other iron and molybdenum. Several molecules of ATP are hydrolyzed during ...
Second Sample Exam
Second Sample Exam

... which of the following pairs of enzymes? A) Glyoxylate synthase and citrate synthase B) Isocitrate lyase and malate synthase C) Rubisco and aldolase D) Aconitase and isocitrate dehydrogenase E) Pyruvate dehydrogenase complex and malate dehydrogenase 3. A new compound is isolated from mitochondria, a ...
Aerobic Metabolism: The Citric Acid Cycle
Aerobic Metabolism: The Citric Acid Cycle

... School of Bioprocess Eng, UniMAP Sem 1, 2012/2013 Week 14: 10/12/2011 ...
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Metabolic network modelling



Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑
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