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Download: 7.2 MB PDF
Download: 7.2 MB PDF

9. Wakil, S. J., Green, DE, Mii, S., and Mahler, HR (1954) Studies on
9. Wakil, S. J., Green, DE, Mii, S., and Mahler, HR (1954) Studies on

... structure of the yeast fatty acid synthase was examined by negative-stain and electron cryomicroscopy. A three-dimensional structure was proposed for the yeast enzyme as a prolate ellipsoid and that the six fatty acid synthesizing centers are composed of two complementary halves - α subunit and a β ...
Amino Acid Sequences and Evolutionary Relationships - Parkway C-2
Amino Acid Sequences and Evolutionary Relationships - Parkway C-2

... # OF DIFFERENCES FROM HUMAN CYTOCHROME c ...
The Acidic Environment #2
The Acidic Environment #2

Enzyme Mechanisms
Enzyme Mechanisms

... It’s one of two energy-rich products of the conversion of light energy into chemical energy in phototrophs ATP then provides drivers for almost everything else other than redox ...
Aspects of Lipid Metabolism in Crustaceans Department of
Aspects of Lipid Metabolism in Crustaceans Department of

... phospholipid fractions of hepatopancreatic lipid From day 7 to day 16, however, there is from normal and destalked G. lateralis. Animals were sacrificed 4 hr after the injection of 10 P.c of no appreciable increase in hepatopanHe-I-acetate into the hemocoel through the arthrocreatic lipid, and the d ...
Ox bile, dried
Ox bile, dried

... salts. The bile salts in fresh bile are mainly totally conjugated as peptides formed from bile acid, glycine or taurine. The selective activity of fully conjugated bile acids is less than that of free acids. Deoxycholic acid is the most active of the bile acids. Bacterial enzymes hydrolyse bile conj ...
International Journal of Tryptophan Research Specificity of the Acute
International Journal of Tryptophan Research Specificity of the Acute

... influence of other neurotransmitters on this firing, it is unlikely that, under acute conditions, these determinants play a primary role in the rapid changes in serotonin synthesis observed during the ATD test. This is because of Trp hydroxylase having a relatively long half-life of 2–3 days, a slow ...
Polynucleotide Phosphorylase and Mitochondrial
Polynucleotide Phosphorylase and Mitochondrial

... Isolated rat liver mitochondria can also rapidly reduce AsV (Németi and Gregus, 2002). Although the contributing enzymes have not been identified, mitochondrial AsV reduction was shown to be sensitive to agents that inhibit oxidative phosphorylation and chemicals that deplete mitochondrial GSH (Né ...
Fatigue During Muscular Exercise
Fatigue During Muscular Exercise

... – With prolonged activity- blood glucose may fall • Fatigue at blood glucose below 3.5 mM ...
Engineering of Aromatic Amino Acid Metabolism in
Engineering of Aromatic Amino Acid Metabolism in

... The microbial production of amino acids dates back to 1957, when a group of scientists isolated a soil bacterium (a Corynebacterium sp.) capable of excreting large amounts of glutamic acid. The main application of glutamic acid in the food industry is in the form of a salt; the flavour enhancer mono ...
GC-content of synonymous codons profoundly influences amino
GC-content of synonymous codons profoundly influences amino

... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
Analysis of Honey Samples - Research Commons@Waikato
Analysis of Honey Samples - Research Commons@Waikato

... and the secondary amino acid proline could not be detected. The method was rejected for these main reasons. ZIC-pHILIC chromatography paired with LC-MS-MS gave high-quality separation of twenty one amino acids, detected using scheduled MRM, in 10 minutes. Amino acid recovery out of vial was low for ...
Divergent evolution of the thiolase superfamily and chalcone
Divergent evolution of the thiolase superfamily and chalcone

... Enzymes of the thiolase superfamily catalyze the formation of carbon–carbon bond via the Claisen condensation reaction. Thiolases catalyze the reversible non-decarboxylative condensation of acetoacetyl-CoA from two molecules of acetyl-CoA, and possess a conserved Cys–His catalytic diad. Elongation e ...
PDF - Geoff McFadden`s Lab
PDF - Geoff McFadden`s Lab

... FNR transfers these electrons to NADP+, thereby creating reduced NADPH, which can be used either to generate ATP or as a cofactor in anabolic reactions. In darkness the reverse can occur, and NADPH is reoxidised by FNR to produce reduced ferredoxin37, which is essential for the activity of several f ...
PFK - ePrints USM
PFK - ePrints USM

Current understanding of fatty acid biosynthesis and the acyl carrier
Current understanding of fatty acid biosynthesis and the acyl carrier

... cycle (Figure 2; step 6). In the first round just described, acetylCoA represents the start of the cycle. At this point the cycle begins again through the condensation reaction of acyl-ACP with another malonyl-ACP group, generated as described above by ACC. The condensation reactions other than the ...
Enzymes:The Catalysts of Life I
Enzymes:The Catalysts of Life I

mitochondrial biogenesis during
mitochondrial biogenesis during

... which they enter a state of dormany called Cysts were washed by floatation in cold saturated NaCl, cryptobiosis . It seems that desiccation of cysts followed by washing with cold water . The cysts were activates the gastrula which, when rehydrated, then incubated in the cold or with agitation at 30° ...


... the formation of compound pyrroline-5-carboxylate (P-5-C) from glutamate or proline (Figure 1). However, the expression of all the enzymes necessary for the synthesis of Arg is restricted to the liver and the intestine mucosa, where citrulline is formed by the action of P-5-C synthetase and ornithin ...
Covalently Bonded Platinum(II) Complexes of [alpha]
Covalently Bonded Platinum(II) Complexes of [alpha]

... 33.8 %, I ˆ 1³2).[7] Chemical shift values and coupling constants are direct consequences of the steric and electronic environment around the observed nuclei, and different values are therefore usually obtained, depending on the R group attached to the organometallic site. Thus, peptide functionaliz ...
Central Role of Glutamate Metabolism in the Maintenance of
Central Role of Glutamate Metabolism in the Maintenance of

... mainly toward glutamate catabolism rather than toward the net synthesis of glutamate, even under hyperammonemia conditions. During hyperammonemia, there is a large increase in cerebral glutamine content, but only small changes in the levels of glutamate and α-ketoglutarate. Thus, the channeling of g ...
Lab Manual Quantitative Analytical Method
Lab Manual Quantitative Analytical Method

1. Sources of cholesterol • diet ~ 30 % • biosynthesis ~ 70% 2. Types
1. Sources of cholesterol • diet ~ 30 % • biosynthesis ~ 70% 2. Types

Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea
Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea

... criteria: (a) fractionation by paper electrophoresis and Amino acid compo8ition of fraction8s IT/2-a 2 The average composition of this group of fractions was paper chromatography revealed only one component; (b) obtained by two methods: (a) by calculation from the; the peptide consisted of amino aci ...
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Citric acid cycle



The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.
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