Amino Acid Sequences of Peptides from a Tryptic Digest of a Urea
... criteria: (a) fractionation by paper electrophoresis and Amino acid compo8ition of fraction8s IT/2-a 2 The average composition of this group of fractions was paper chromatography revealed only one component; (b) obtained by two methods: (a) by calculation from the; the peptide consisted of amino aci ...
... criteria: (a) fractionation by paper electrophoresis and Amino acid compo8ition of fraction8s IT/2-a 2 The average composition of this group of fractions was paper chromatography revealed only one component; (b) obtained by two methods: (a) by calculation from the; the peptide consisted of amino aci ...
organonitrogen compounds i. amines
... There is, though, a major difference in the way that amines and alcohols behave toward oxidizing agents. Amines generally show more complex behavior on oxidation because, as we shall see, nitrogen has a larger number of stable ...
... There is, though, a major difference in the way that amines and alcohols behave toward oxidizing agents. Amines generally show more complex behavior on oxidation because, as we shall see, nitrogen has a larger number of stable ...
Lactobacillus plantarum - UEF Electronic Publications
... range of applications where this bacterium has been used makes research on this topic especially interesting and challenging. For this reason, two strains of Lactobacillus plantarum from different origin (REB1 and MLBPL1, the latter from vegetable material) were used in proteomics studies in order t ...
... range of applications where this bacterium has been used makes research on this topic especially interesting and challenging. For this reason, two strains of Lactobacillus plantarum from different origin (REB1 and MLBPL1, the latter from vegetable material) were used in proteomics studies in order t ...
Enzymes
... 2. Group Specificity - working upon a related group of molecules containing a specific functional group. 3. Linkage Specificity - working on molecules that contain a specific type of chemical bond. 2. Enzymes are Stereospecific. If a molecule exists as a pair of enantiomers, the enzyme will use only ...
... 2. Group Specificity - working upon a related group of molecules containing a specific functional group. 3. Linkage Specificity - working on molecules that contain a specific type of chemical bond. 2. Enzymes are Stereospecific. If a molecule exists as a pair of enantiomers, the enzyme will use only ...
1 oxidative capacity distribution in skeletal muscle fibers of the rat
... that the oxidative capacity of the mitochondria is different in different regions of the muscle fiber. In heart muscle, Palmer et al. (1977, 1985) and McMillin-Wood et al. (1980) have described two populations of mitochondria with different oxidative characteristics. One of them, released by using t ...
... that the oxidative capacity of the mitochondria is different in different regions of the muscle fiber. In heart muscle, Palmer et al. (1977, 1985) and McMillin-Wood et al. (1980) have described two populations of mitochondria with different oxidative characteristics. One of them, released by using t ...
Rutile titanium dioxide nanoparticles and ordered acicular
... Alfa Aesar), 97 g HCl solution (37% from Fisher Scienti?c), and 397 g of titanium oxychloride solution (25.2% in TiOZ, from Millennium Inorganic Chemicals) were mixed together ...
... Alfa Aesar), 97 g HCl solution (37% from Fisher Scienti?c), and 397 g of titanium oxychloride solution (25.2% in TiOZ, from Millennium Inorganic Chemicals) were mixed together ...
as a PDF
... higher pH, and markedly greater long-term survival of lactococci (12, 16). However, the mechanisms involved in this stimulation are not yet understood. One reason is that only a limited number of genetic components involved in respiration growth have been identified (12). In contrast, bacteria descr ...
... higher pH, and markedly greater long-term survival of lactococci (12, 16). However, the mechanisms involved in this stimulation are not yet understood. One reason is that only a limited number of genetic components involved in respiration growth have been identified (12). In contrast, bacteria descr ...
Journal of Microbiology and Biotechnology
... have been reported to be produced by rhizobacteria [30]. IAA synthesized by plant-associated bacteria is implicated as one of the key determinants in association of plantmicrobe interaction, stimulation of plant growth, and plant pathogenesis. In many cases, a single bacterial strain may possess mor ...
... have been reported to be produced by rhizobacteria [30]. IAA synthesized by plant-associated bacteria is implicated as one of the key determinants in association of plantmicrobe interaction, stimulation of plant growth, and plant pathogenesis. In many cases, a single bacterial strain may possess mor ...
1. Introduction to Natural Products Chemistry
... materials, and forming products such as glucose, amino acids, nucleic acids. These are similar among different species. • In secondary metabolism, the biosynthetic steps, substrates and products are characteristic of families and species. Species which are taxonomically close display greater similar ...
... materials, and forming products such as glucose, amino acids, nucleic acids. These are similar among different species. • In secondary metabolism, the biosynthetic steps, substrates and products are characteristic of families and species. Species which are taxonomically close display greater similar ...
Milk Fat: Origin of Fatty Acids and Influence of
... TAGs. Because the long-chain fatty acids comprise approximately 50% of milk fat, this observation implied that more than 90% of these fatty acids were of plasma origin, consistent with the demonstration of little fatty acid elongation in the mammary gland (Palmquist et al., 1969). Finally, Glascock ...
... TAGs. Because the long-chain fatty acids comprise approximately 50% of milk fat, this observation implied that more than 90% of these fatty acids were of plasma origin, consistent with the demonstration of little fatty acid elongation in the mammary gland (Palmquist et al., 1969). Finally, Glascock ...
Peroxidases and Catalases. Biochemistry, Biophysics, Biotechnology and Physiology Brochure
... and Catalases: Biochemistry, Biophysics, Biotechnology and Physiology provides a much–needed systematic, up–to–date treatment of peroxidases and catalases. From the structure and properties of the various superfamilies to current applications of peroxidases, the book consolidates vast amounts of inf ...
... and Catalases: Biochemistry, Biophysics, Biotechnology and Physiology provides a much–needed systematic, up–to–date treatment of peroxidases and catalases. From the structure and properties of the various superfamilies to current applications of peroxidases, the book consolidates vast amounts of inf ...
Acid CleavageLDeprotection in Fmoc/tBu Solid
... Any Cys(Acm) as part of the peptide chain on the resin remains unchanged, i.e., Cys will not be deprotected. Commercially available with the first (C-terminal)-protected amino acid linked to the resin (from BACHEM Feinchemikalien AG, Switzerland). Commercially available with Fmoc protection, i.e., w ...
... Any Cys(Acm) as part of the peptide chain on the resin remains unchanged, i.e., Cys will not be deprotected. Commercially available with the first (C-terminal)-protected amino acid linked to the resin (from BACHEM Feinchemikalien AG, Switzerland). Commercially available with Fmoc protection, i.e., w ...
Clinical Application of Blood Ammonia Determinations
... The proper functioning of the urea cycle requires adequate levels of ornithine. If dietary arginine is low, ornithine must be produced. If dietary arginine and ornithine are in excess, they can be converted to glutamate. Production and degradation of ornithine occurs by a two-step process catalyzed ...
... The proper functioning of the urea cycle requires adequate levels of ornithine. If dietary arginine is low, ornithine must be produced. If dietary arginine and ornithine are in excess, they can be converted to glutamate. Production and degradation of ornithine occurs by a two-step process catalyzed ...
Mammalian Cell Culture: High Throughput Applications of
... lines derived from various organs and organisms are now commercially available. The cell lines can either be adherent, i.e. requiring a support for growth or can grow in suspension. The Chinese hamster ovary (CHO) cell line was developed at the end of the 1950 (Puck et al., 1958) and it is available ...
... lines derived from various organs and organisms are now commercially available. The cell lines can either be adherent, i.e. requiring a support for growth or can grow in suspension. The Chinese hamster ovary (CHO) cell line was developed at the end of the 1950 (Puck et al., 1958) and it is available ...
- Journal of Hepatology
... indication of an increased turnover of phosphatidylcholine and phosphatidylethanolamine species in the liver, thus releasing free fatty acids through the action of phospholipases A1 and A2. These fatty acids, if not catabolized by b-oxidation, will be stored in the liver as triacylglycerols. This is ...
... indication of an increased turnover of phosphatidylcholine and phosphatidylethanolamine species in the liver, thus releasing free fatty acids through the action of phospholipases A1 and A2. These fatty acids, if not catabolized by b-oxidation, will be stored in the liver as triacylglycerols. This is ...
inclusion of a glycogen regulation mathematical model into a
... biology point of view through mathematical modeling is daunting but very important as there has been a drastic increase in the prevalence of obesity, diabetes and other concerns relating to metabolism. By obtaining a better understanding of metabolic processes, especially during transitional feeding ...
... biology point of view through mathematical modeling is daunting but very important as there has been a drastic increase in the prevalence of obesity, diabetes and other concerns relating to metabolism. By obtaining a better understanding of metabolic processes, especially during transitional feeding ...
respiration
... used as an insulator to prevent heat loss so that the result is more easily observed. ...
... used as an insulator to prevent heat loss so that the result is more easily observed. ...
Metabolic effects of glutamine on insulin sensitivity
... gluconeogenic amino acid (18, 19). Gln basal turnover in the postabsorptive state of normal subjects is greater than that of alanine (20, 21). In vivo and in vitro studies showed that Gln is the major gluconeogenic precursor in the kidney. The Gln carbon skeleton derives mainly from other amino acid ...
... gluconeogenic amino acid (18, 19). Gln basal turnover in the postabsorptive state of normal subjects is greater than that of alanine (20, 21). In vivo and in vitro studies showed that Gln is the major gluconeogenic precursor in the kidney. The Gln carbon skeleton derives mainly from other amino acid ...
enzymes lecture 3
... Many enzymes depend on free sulphahydryl group for its activity. Inhibition of this group leads to loss of the enzyme activity. ...
... Many enzymes depend on free sulphahydryl group for its activity. Inhibition of this group leads to loss of the enzyme activity. ...
Peroxisomes and peroxisomal disorders: The main facts
... thiolytic cleavage. After each cycle, fatty acids are shortened of two carbon atoms which are released as acetyl-CoA (Lazarow and De Duve 1976; Rinaldo et al., 2002; Wanders, 2004) (Figure ...
... thiolytic cleavage. After each cycle, fatty acids are shortened of two carbon atoms which are released as acetyl-CoA (Lazarow and De Duve 1976; Rinaldo et al., 2002; Wanders, 2004) (Figure ...
Communication
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
Regulation of Ketone Body Metabolism and the Role of PPAR
... in the systemic metabolism during fasting is triggered by glucagon and involves the mobilization of lipids stored in adipose tissue and break down of triglycerides to free fatty acids and glycerol [1,2]. These two components are subsequently catabolized in liver: fatty acids undergo β-oxidation to p ...
... in the systemic metabolism during fasting is triggered by glucagon and involves the mobilization of lipids stored in adipose tissue and break down of triglycerides to free fatty acids and glycerol [1,2]. These two components are subsequently catabolized in liver: fatty acids undergo β-oxidation to p ...
Laboratory Evolution of Cytochrome P450 BM-3 Monooxygenase for Organic Cosolvents
... Point mutagenesis at low error rates explores only a very limited set of (primarily conservative) amino acid substitutions. We and others have observed that saturation mutagenesis performed at sites identified by error-prone PCR often generates further improvements. Taking double-mutant F87AB5 as th ...
... Point mutagenesis at low error rates explores only a very limited set of (primarily conservative) amino acid substitutions. We and others have observed that saturation mutagenesis performed at sites identified by error-prone PCR often generates further improvements. Taking double-mutant F87AB5 as th ...
CP1 Domain in Escherichia coli Leucyl
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
Citric acid cycle
The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.