Life and death of transcriptional co
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
A low-molecular-mass protein from Methylococcus
... isolation and that NAD may also be associated with the enzyme but in a form that is less tightly bound than found with the methanol dehydrogenase from Bacillus methanolicus. Data are presented which indicate that the modifin may play an important role in regulating formaldehyde concentration in vivo ...
... isolation and that NAD may also be associated with the enzyme but in a form that is less tightly bound than found with the methanol dehydrogenase from Bacillus methanolicus. Data are presented which indicate that the modifin may play an important role in regulating formaldehyde concentration in vivo ...
Meiotic Recombination inSchizosaccharomyces pombe: A Paradigm
... Homologous genetic recombination plays two important roles during meiosis, the special nuclear divisions during which chromosome number is reduced from two (diploid) to one (haploid). First, recombination provides the physical connection between homologs that aids their pairing and proper segregatio ...
... Homologous genetic recombination plays two important roles during meiosis, the special nuclear divisions during which chromosome number is reduced from two (diploid) to one (haploid). First, recombination provides the physical connection between homologs that aids their pairing and proper segregatio ...
VAAM-Jahrestagung 2016 13.–16. März in Jena
... The LightMix® Kit Bordetella pertussis and parapertussis Real-Time-PCR genome detection ... provides quick results in less than an hour ... is based on PCR based methods that allow the diagnosis ...
... The LightMix® Kit Bordetella pertussis and parapertussis Real-Time-PCR genome detection ... provides quick results in less than an hour ... is based on PCR based methods that allow the diagnosis ...
From filaments to function: The role of the plant actin cytoskeleton in
... targeting of this network, the actin-endomembrane systems have been characterized through work demonstrating actindependent hijacking of the ER by the plant enveloped virus Tomato spotted wilt tospovirus (TSVW; (Feng et al. 2013; Ribeiro et al. 2013); Figure 2A; Table 1). In brief, the TSWV membrane ...
... targeting of this network, the actin-endomembrane systems have been characterized through work demonstrating actindependent hijacking of the ER by the plant enveloped virus Tomato spotted wilt tospovirus (TSVW; (Feng et al. 2013; Ribeiro et al. 2013); Figure 2A; Table 1). In brief, the TSWV membrane ...
Introduction Wnt signaling in development
... related Wnt/PCP pathway is required for the convergence and extension cell movements that drive gastrulation in vertebrates (Myers et al., 2002). Another non-canonical Wnt pathway involves the atypical receptor tyrosine kinase ROR2, which contains a cysteine-rich Wnt binding domain (Masiakowski and ...
... related Wnt/PCP pathway is required for the convergence and extension cell movements that drive gastrulation in vertebrates (Myers et al., 2002). Another non-canonical Wnt pathway involves the atypical receptor tyrosine kinase ROR2, which contains a cysteine-rich Wnt binding domain (Masiakowski and ...
of Lactobacillus pentosus
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
Identification and characterization of regulatory factors and
... 3.1.1 YmoA activates rovA expression by downregulating RovM ..................................57 3.1.2 YmoA represses rovM expression through the Csr system ....................................61 3.1.3 YmoA regulates CsrC synthesis independently of CsrB ........................................6 ...
... 3.1.1 YmoA activates rovA expression by downregulating RovM ..................................57 3.1.2 YmoA represses rovM expression through the Csr system ....................................61 3.1.3 YmoA regulates CsrC synthesis independently of CsrB ........................................6 ...
Upregulation of Alveolar Epithelial Active Na Transport Is
... Abstract—Alveolar epithelial -adrenergic receptor (AR) activation accelerates active Na⫹ transport in lung epithelial cells in vitro and speeds alveolar edema resolution in human lung tissue and normal and injured animal lungs. Whether these receptors are essential for alveolar fluid clearance (AF ...
... Abstract—Alveolar epithelial -adrenergic receptor (AR) activation accelerates active Na⫹ transport in lung epithelial cells in vitro and speeds alveolar edema resolution in human lung tissue and normal and injured animal lungs. Whether these receptors are essential for alveolar fluid clearance (AF ...
Paired and LIM class homeodomain proteins coordinate
... The ancient origin of sleep is evidenced by deeply conserved signaling pathways regulating sleep-like behavior, such as signaling through the Epidermal growth factor receptor (EGFR). In Caenorhabditis elegans, a sleep-like state can be induced at any time during development or adulthood through cond ...
... The ancient origin of sleep is evidenced by deeply conserved signaling pathways regulating sleep-like behavior, such as signaling through the Epidermal growth factor receptor (EGFR). In Caenorhabditis elegans, a sleep-like state can be induced at any time during development or adulthood through cond ...
Organization of Physical Interactomes as
... We also uncover S. cerevisiae emergent pair schemas using a hand-chosen set of GO molecular function annotations (Figure 2B and Tables S1 and S6). As with the Pfam schemas, the GO pair schemas represent many types of functions including transport, signaling, DNA and RNA processing, ubiquitination, p ...
... We also uncover S. cerevisiae emergent pair schemas using a hand-chosen set of GO molecular function annotations (Figure 2B and Tables S1 and S6). As with the Pfam schemas, the GO pair schemas represent many types of functions including transport, signaling, DNA and RNA processing, ubiquitination, p ...
View PDF - Genes | Genomes | Genetics
... expression using RNA in situ hybridization (Figure S4). These dissections, as well as the dissections used to obtain tissue for the individual limb bud western blots, were performed in an identical manner. These experiments demonstrated that the entire limb domain of Shh expression was captured in t ...
... expression using RNA in situ hybridization (Figure S4). These dissections, as well as the dissections used to obtain tissue for the individual limb bud western blots, were performed in an identical manner. These experiments demonstrated that the entire limb domain of Shh expression was captured in t ...
The role of c-di-GMP signaling in an Aeromonas veronii biovar
... pSB536, which preferentially detects N-(butanoyl)-L-homoserine lactone (C4-HSL), was used (Swift et al., 1997; Winson et al., 1998). To estimate the amount of HSL in the culture, a standard curve of relative light units as a function of C4-HSL concentration was constructed using E. coli JM109 (pSB53 ...
... pSB536, which preferentially detects N-(butanoyl)-L-homoserine lactone (C4-HSL), was used (Swift et al., 1997; Winson et al., 1998). To estimate the amount of HSL in the culture, a standard curve of relative light units as a function of C4-HSL concentration was constructed using E. coli JM109 (pSB53 ...
A PP2A-B55 recognition signal controls substrate
... Hunt, 2012; Grallert et al., 2015). Although there has been considerable focus on understanding the regulatory networks controlling kinase and phosphatase activity (Novak et al., 2010; Ferrell, 2013), how individual substrates for the same pathway are differentially regulated is still poorly underst ...
... Hunt, 2012; Grallert et al., 2015). Although there has been considerable focus on understanding the regulatory networks controlling kinase and phosphatase activity (Novak et al., 2010; Ferrell, 2013), how individual substrates for the same pathway are differentially regulated is still poorly underst ...
Acyl-CoA oxidase is imported as a heteropentameric, cofactor
... membrane targeting of the complex, while other components are not required for any of these functions. Such a division of labor amongst different components has recently been reported for the heterodimeric hydrogenase 2 enzyme translocated across the bacterial plasma membrane by the Tat pathway (Rod ...
... membrane targeting of the complex, while other components are not required for any of these functions. Such a division of labor amongst different components has recently been reported for the heterodimeric hydrogenase 2 enzyme translocated across the bacterial plasma membrane by the Tat pathway (Rod ...
A Single Eubacterial Origin of Eukaryotic
... eight PFO sequences, including those from the anaerobic eukaryotes Entamoeba histolytica, Giardia lamblia, and Trichomonas vaginalis. They concluded that there was little support for a common ancestry of eukaryote PFO and that Entamoeba probably got its PFO independent of the other eukaryotes, and p ...
... eight PFO sequences, including those from the anaerobic eukaryotes Entamoeba histolytica, Giardia lamblia, and Trichomonas vaginalis. They concluded that there was little support for a common ancestry of eukaryote PFO and that Entamoeba probably got its PFO independent of the other eukaryotes, and p ...
The Differential Degradation of Two Cytosolic Proteins As a Tool to
... (1990), who followed sequential steps in autophagy after inducing artificial protein complexes in the ER. However, their observations were restricted to the degradation of these complexes while, in general, autophagy is thought to be a nonspecific process in which cytoplasmic proteins are degraded r ...
... (1990), who followed sequential steps in autophagy after inducing artificial protein complexes in the ER. However, their observations were restricted to the degradation of these complexes while, in general, autophagy is thought to be a nonspecific process in which cytoplasmic proteins are degraded r ...
Pathogenesis in tuberculosis: transcriptomic
... cells, during acidification and mycobacterial phagosome maturation (Russell et al., 1996; Schaible et al., 1998). However, it is unclear whether the observed mycobacterial phagosome acidification and maturation is the cause or effect of the killing of M. tuberculosis, as these treatments activate ma ...
... cells, during acidification and mycobacterial phagosome maturation (Russell et al., 1996; Schaible et al., 1998). However, it is unclear whether the observed mycobacterial phagosome acidification and maturation is the cause or effect of the killing of M. tuberculosis, as these treatments activate ma ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Members of the Rho family of small GTPases have been shown to be important regulators of cellular behavior, especially actin cytoskeletal organization and acto-myosin based contractility (Hall, 1998). Rho proteins function as molecular switches, cycling between an active GTP-bound state and an inact ...
... Members of the Rho family of small GTPases have been shown to be important regulators of cellular behavior, especially actin cytoskeletal organization and acto-myosin based contractility (Hall, 1998). Rho proteins function as molecular switches, cycling between an active GTP-bound state and an inact ...
Prions as protein-based genetic elements. - Lindquist Lab
... state in living cells, Rnq1 or certain prion-determining fragments of Sup35 were fused to green fluorescent protein (GFP) and expressed in yeast. In cells without either prion, these GFP fusion proteins distribute evenly throughout the cytoplasm (122, 147). In [PSI+] or [RNQ+] cells, such fusion pro ...
... state in living cells, Rnq1 or certain prion-determining fragments of Sup35 were fused to green fluorescent protein (GFP) and expressed in yeast. In cells without either prion, these GFP fusion proteins distribute evenly throughout the cytoplasm (122, 147). In [PSI+] or [RNQ+] cells, such fusion pro ...
C. elegans and volatile anesthetics
... over four to five orders of magnitude of lipid solubility (Meyer, 1899; Overton, 1901; Tanifuji et al., 1977). Termed the Meyer-Overton rule, the correlation led to early hypotheses about how volatile anesthetics work. A plot of this relationship could be used to derive an interesting corollary. Ass ...
... over four to five orders of magnitude of lipid solubility (Meyer, 1899; Overton, 1901; Tanifuji et al., 1977). Termed the Meyer-Overton rule, the correlation led to early hypotheses about how volatile anesthetics work. A plot of this relationship could be used to derive an interesting corollary. Ass ...
Distinct Cellular and Molecular Mechanisms Underlie Functional
... Subsequent experiments examined the impact of TACinduced LVH on the transcript and protein expression levels of several channel pore-forming (␣) and accessory () subunits encoding murine myocardial K⫹ channels.14,19,22–24 Because accumulating evidence suggests that cardiac ion channels function as ...
... Subsequent experiments examined the impact of TACinduced LVH on the transcript and protein expression levels of several channel pore-forming (␣) and accessory () subunits encoding murine myocardial K⫹ channels.14,19,22–24 Because accumulating evidence suggests that cardiac ion channels function as ...
informe tecnológico de patentes
... AB - Huntington's disease (HD) is a dominantly inherited neurodegenerative disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in t ...
... AB - Huntington's disease (HD) is a dominantly inherited neurodegenerative disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in t ...
Endogenous Cerberus activity is required for anterior head
... observed when the 5′ UTR cerberus mRNA and the CerMo were coinjected (Fig. 1F), demonstrating that the morpholino can efficiently inhibit Cerberus activity in the embryo. In order to assess the phenotypic effect of knocking down endogenous Cerberus, four- to eight-cell stage embryos were injected wi ...
... observed when the 5′ UTR cerberus mRNA and the CerMo were coinjected (Fig. 1F), demonstrating that the morpholino can efficiently inhibit Cerberus activity in the embryo. In order to assess the phenotypic effect of knocking down endogenous Cerberus, four- to eight-cell stage embryos were injected wi ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.