Alternative splicing of human cyclin E - Journal of Cell Science
... isolated the smaller amplified fragment by agarose gel electrophoresis and cloned it into pBluescript KS. The DNA sequence of the insert was determined and compared to the corresponding region of cyclin E. The sequence of the relevant region around the splice junction is shown in Fig. 2a and b. It i ...
... isolated the smaller amplified fragment by agarose gel electrophoresis and cloned it into pBluescript KS. The DNA sequence of the insert was determined and compared to the corresponding region of cyclin E. The sequence of the relevant region around the splice junction is shown in Fig. 2a and b. It i ...
Role of histone deacetylase 2 and its
... without correction, the function of the hypertrophied heart declines, leading to heart failure. Despite intensive management, heart failure is still one of the most common causes of death (4). To acquire proper functions, many proteins undergo modification after translation by enzymes, a process re ...
... without correction, the function of the hypertrophied heart declines, leading to heart failure. Despite intensive management, heart failure is still one of the most common causes of death (4). To acquire proper functions, many proteins undergo modification after translation by enzymes, a process re ...
6. risk management plan
... Transfer of genes to other cotton plants ..................................................................... 54 Transfer of genes to other plant species ..................................................................... 54 B: Likelihood of the gene transfer hazard occurring .................... ...
... Transfer of genes to other cotton plants ..................................................................... 54 Transfer of genes to other plant species ..................................................................... 54 B: Likelihood of the gene transfer hazard occurring .................... ...
Selective Disruption of Aurora C Kinase Reveals Distinct Functions
... generating cells with the proper number of chromosomes. In germ cells, sperm and egg, an abnormal chromosome number leads to infertility, miscarriage, or, in the case of a live birth, developmental disorders such as Down Syndrome. For reasons not entirely clear, eggs are more prone to chromosome seg ...
... generating cells with the proper number of chromosomes. In germ cells, sperm and egg, an abnormal chromosome number leads to infertility, miscarriage, or, in the case of a live birth, developmental disorders such as Down Syndrome. For reasons not entirely clear, eggs are more prone to chromosome seg ...
Polar auxin transport – old questions and new concepts?
... analyses demonstrate that PID action is sensitive to AEI thus favouring the hypothesis that PID functions as a positive regulator of auxin transport (Benjamins et al., 2001). Thus analysis of both RCN and PID highlights a role of phosphorylation in the regulation of PAT. The tir (transport inhibitor ...
... analyses demonstrate that PID action is sensitive to AEI thus favouring the hypothesis that PID functions as a positive regulator of auxin transport (Benjamins et al., 2001). Thus analysis of both RCN and PID highlights a role of phosphorylation in the regulation of PAT. The tir (transport inhibitor ...
Cell Calcium Viral calciomics: Interplays between Ca2+ and virus
... activation of IP3 R and release of Ca2+ from internal store. The decrease of ER Ca2+ concentration is subsequently sensed by the EF-hand-containing ER Ca2+ sensor STIM1, which in turn activates the CRAC channels through its direct interaction with the pore-forming subunit Orai1, followed by a second ...
... activation of IP3 R and release of Ca2+ from internal store. The decrease of ER Ca2+ concentration is subsequently sensed by the EF-hand-containing ER Ca2+ sensor STIM1, which in turn activates the CRAC channels through its direct interaction with the pore-forming subunit Orai1, followed by a second ...
ABSTRACT Title of Document:
... Importance and history of the ethylene pathway Plants are sessile organisms that must respond quickly to a vast array of external and internal cues such as light, gravity, temperature, pathogen attack, and hormones. The speed and accuracy of response is vital to its development and survival. The com ...
... Importance and history of the ethylene pathway Plants are sessile organisms that must respond quickly to a vast array of external and internal cues such as light, gravity, temperature, pathogen attack, and hormones. The speed and accuracy of response is vital to its development and survival. The com ...
Substrate specificity and inhibitors of LRRK2, a protein kinase
... of the LRRKtide peptide that encompasses the Thr567 ERM phosphorylation motif (Figure 1C). The wild-type LRRKtide peptide was phosphorylated by LRRK2 with a K m of 200 μM and V max of 14 units/mg. The following mutations to alanine suppressed phosphorylation by increasing the K m value: Arg−5 (2.5-f ...
... of the LRRKtide peptide that encompasses the Thr567 ERM phosphorylation motif (Figure 1C). The wild-type LRRKtide peptide was phosphorylated by LRRK2 with a K m of 200 μM and V max of 14 units/mg. The following mutations to alanine suppressed phosphorylation by increasing the K m value: Arg−5 (2.5-f ...
Modified uridine at wobble position in tRNA of
... facilitated the identification of genes required for tRNA modification. So far, in S. cerevisiae 60 genes have been identified. These gene products include tRNA modifying enzymes as well as the proteins that in other ways are required for synthesis of the modified nucleosides (Table 1; Paper II). ...
... facilitated the identification of genes required for tRNA modification. So far, in S. cerevisiae 60 genes have been identified. These gene products include tRNA modifying enzymes as well as the proteins that in other ways are required for synthesis of the modified nucleosides (Table 1; Paper II). ...
as Hotspot Ca 2+ Signaling Units
... channel of still debated nature localized in the IMM [34], can be also considered as a non-saturating mechanism for Ca2+ efflux from mitochondria. When open, PTP allows the passage of ions and molecules with a molecular weight up to 1.5 kDa, including Ca2+. Short-time openings may have a physiologic ...
... channel of still debated nature localized in the IMM [34], can be also considered as a non-saturating mechanism for Ca2+ efflux from mitochondria. When open, PTP allows the passage of ions and molecules with a molecular weight up to 1.5 kDa, including Ca2+. Short-time openings may have a physiologic ...
View/Open - eDiss - Georg-August
... to release a soluble ectodomain derivative into the apoplast. The ectodomain fragment is likely to be generated by a proteolytic mechanism called ectodomain shedding. Ectodomain shedding is well documented in animals, where it fulfils diverse regulatory functions on a range of different proteins. In ...
... to release a soluble ectodomain derivative into the apoplast. The ectodomain fragment is likely to be generated by a proteolytic mechanism called ectodomain shedding. Ectodomain shedding is well documented in animals, where it fulfils diverse regulatory functions on a range of different proteins. In ...
Literature Study
... of ASD are classical autism, Asperger syndrome and pervasive developmental disorder-not otherwise specified (PDD-NOS)(Baltussen, Clijsen, Leenders 2003). Individuals with an ASD can be characterized by impairment in social relationships, atypical verbal and non-verbal communication, difficulties wit ...
... of ASD are classical autism, Asperger syndrome and pervasive developmental disorder-not otherwise specified (PDD-NOS)(Baltussen, Clijsen, Leenders 2003). Individuals with an ASD can be characterized by impairment in social relationships, atypical verbal and non-verbal communication, difficulties wit ...
An N-terminally truncated envelope protein encoded
... Env protein encoded by Xq22.3 HERV-W env is shown in Figure 1B. To analyze the coding capacity of Xq22.3 HERV-W env, we PCR-amplified from human genomic DNA a 1862 bp sequence beginning at the ATG at codon 68 of Xq22.3 HERV-W env and containing the putative 475 amino acid HERV-W env ORF as well as t ...
... Env protein encoded by Xq22.3 HERV-W env is shown in Figure 1B. To analyze the coding capacity of Xq22.3 HERV-W env, we PCR-amplified from human genomic DNA a 1862 bp sequence beginning at the ATG at codon 68 of Xq22.3 HERV-W env and containing the putative 475 amino acid HERV-W env ORF as well as t ...
PDF - Multinational Arabidopsis Steering Committee
... should stand next to your poster, find your abstract in this book and note the new abstract number. The new number is your poster number, NOT the number it was assigned when you originally submitted. Posters are sequenced by topic and presenter. See the poster list below to determine which group con ...
... should stand next to your poster, find your abstract in this book and note the new abstract number. The new number is your poster number, NOT the number it was assigned when you originally submitted. Posters are sequenced by topic and presenter. See the poster list below to determine which group con ...
Protein phosphatase 1 down regulates ZYG
... Protein phosphatase 1 (PP1) is a major cellular phosphatase that plays well-characterized roles in diverse processes including glycogen metabolism, circadian rhythms and cell division. Humans possess a single PP1α gene, a single PP1β gene (also known as PP1δ) and a singl ...
... Protein phosphatase 1 (PP1) is a major cellular phosphatase that plays well-characterized roles in diverse processes including glycogen metabolism, circadian rhythms and cell division. Humans possess a single PP1α gene, a single PP1β gene (also known as PP1δ) and a singl ...
Endoplasmic reticulum localization of the low density lipoprotein
... hybridoma-derived tissue culture medium), where as endogenous LDLR was detected by using a polyclonal rabbit antihuman LDLR354 Ab (10) (1:300 dilution). Hepatocytes were incubated with primary Abs on ice for 1 h and washed twice before secondary Ab addition. Polyclonal anti-LDLR Ab was detected by u ...
... hybridoma-derived tissue culture medium), where as endogenous LDLR was detected by using a polyclonal rabbit antihuman LDLR354 Ab (10) (1:300 dilution). Hepatocytes were incubated with primary Abs on ice for 1 h and washed twice before secondary Ab addition. Polyclonal anti-LDLR Ab was detected by u ...
The hyaluronan lyase of Streptococcus pyogenes bacteriophage
... Hyaluronan (HA) lyases are produced by many different genera of bacteria. In Gram-positive organisms, the HA lyase is often secreted and is thought to have a role in pathogenesis. It has been noted [1] that all Gram-positive bacteria which produce HA lyases appear to be capable of causing infections ...
... Hyaluronan (HA) lyases are produced by many different genera of bacteria. In Gram-positive organisms, the HA lyase is often secreted and is thought to have a role in pathogenesis. It has been noted [1] that all Gram-positive bacteria which produce HA lyases appear to be capable of causing infections ...
Microbiology
... The Edw. ictaluri host factors that modulate phage–host interactions have not been described previously. This study identified eleven unique Edw. ictaluri host factors essential for phage infection by screening a transposon mutagenized library of two Edw. ictaluri strains for phageresistant mutants. ...
... The Edw. ictaluri host factors that modulate phage–host interactions have not been described previously. This study identified eleven unique Edw. ictaluri host factors essential for phage infection by screening a transposon mutagenized library of two Edw. ictaluri strains for phageresistant mutants. ...
MEF2 and HDAC Proteins Regulate Striated Muscle
... The MEF2 family.............................................................................................................. 37 Regulation of MEF2 activity by post-translational modifications.................................... 40 Modulation of MEF2 activity by class II HDACs ...................... ...
... The MEF2 family.............................................................................................................. 37 Regulation of MEF2 activity by post-translational modifications.................................... 40 Modulation of MEF2 activity by class II HDACs ...................... ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... D.Bimston and J.Song contributed equally to this work ...
... D.Bimston and J.Song contributed equally to this work ...
TATA BINDING PROTEIN AND ASSOCIATED FACTORS ... TRANSCRIPTION: CHARACTERIZATION OF THE B-TFIID ACTIVITY
... required in addition to RNApol I to reconstitute accurate and promoter specific pol I transcription (Learned et al., 1985; Learned et al., 1986; Bell et al., 1988). UBFis a DNAbinding protein that interacts with both the UCEand the core region of the rRNApromoter. UBFis the only sequence specific DN ...
... required in addition to RNApol I to reconstitute accurate and promoter specific pol I transcription (Learned et al., 1985; Learned et al., 1986; Bell et al., 1988). UBFis a DNAbinding protein that interacts with both the UCEand the core region of the rRNApromoter. UBFis the only sequence specific DN ...
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics
... Marschalek R. MLL (myeloid/lymphoid or mixed lineage leukemia). Atlas Genet Cytogenet Oncol Haematol.2003;7(1):16-18. Hess JL, Huret JL. MLL (myeloid/lymphoid or mixed lineage leukemia). Atlas Genet Cytogenet Oncol Haematol.2001;5(1):12-14. Huret JL. MLL (myeloid/lymphoid or mixed lineage leukemia). ...
... Marschalek R. MLL (myeloid/lymphoid or mixed lineage leukemia). Atlas Genet Cytogenet Oncol Haematol.2003;7(1):16-18. Hess JL, Huret JL. MLL (myeloid/lymphoid or mixed lineage leukemia). Atlas Genet Cytogenet Oncol Haematol.2001;5(1):12-14. Huret JL. MLL (myeloid/lymphoid or mixed lineage leukemia). ...
PKC modulates NF-B and AP-1 via mitogen
... signaling molecule is necessary for late PC to become manifest. Although PKC⑀ appears to play an essential role in ischemic PC (15, 23, 32, 33), the downstream signaling events triggered by the activation of this specific isozyme during the development of the late phase of PC remain largely unknown. ...
... signaling molecule is necessary for late PC to become manifest. Although PKC⑀ appears to play an essential role in ischemic PC (15, 23, 32, 33), the downstream signaling events triggered by the activation of this specific isozyme during the development of the late phase of PC remain largely unknown. ...
Retinoblastoma Protein Contains a C-terminal - Bio
... requirement for a (serine/threonine)-proline (S/T-P) phosphoacceptor site and a preference for a basic residue at position 13 (where the S/T position is position 0) (36, 70, 83, 85, 98). It is clear, however, that the cyclin moiety contributes to substrate specificity. For example, cyclin A-cdc2 but ...
... requirement for a (serine/threonine)-proline (S/T-P) phosphoacceptor site and a preference for a basic residue at position 13 (where the S/T position is position 0) (36, 70, 83, 85, 98). It is clear, however, that the cyclin moiety contributes to substrate specificity. For example, cyclin A-cdc2 but ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.