Purified dextransucrase from Pediococcus pentosaceus CRAG3 as
... Glucansucrases (GSs) are large size extracellular enzymes that catalyse the synthesis of different types of α-glucans such as dextran, mutan, alternan and reuteran using sucrose as a substrate. Depending upon the nature of synthesized product they are categorize as dextransucrase (EC 2.4.1.5), alter ...
... Glucansucrases (GSs) are large size extracellular enzymes that catalyse the synthesis of different types of α-glucans such as dextran, mutan, alternan and reuteran using sucrose as a substrate. Depending upon the nature of synthesized product they are categorize as dextransucrase (EC 2.4.1.5), alter ...
AtREM1, a Member of a New Family of B3 Domain
... (Franco-Zorrilla et al., 1999), AtREM1 (REM1) also belongs to a gene family in Arabidopsis. Genomic DNA sequences from the Arabidopsis Genome Initiative have revealed the existence of at least 23 sequences characterized by the presence of duplicated REM domains (Table I; Fig. 2). Many of them also h ...
... (Franco-Zorrilla et al., 1999), AtREM1 (REM1) also belongs to a gene family in Arabidopsis. Genomic DNA sequences from the Arabidopsis Genome Initiative have revealed the existence of at least 23 sequences characterized by the presence of duplicated REM domains (Table I; Fig. 2). Many of them also h ...
The Molecular Basis of Diversity in the
... flowering under LDs and reduced FT levels (Johnson et al., 1994; Guo et al., 1998; Yanovsky and Kay, 2002). Particularly, loss of CRY2 function delays flowering without affecting the diurnal pattern of CO mRNA (Yanovsky and Kay, 2002). In contrast, phyB mutations result in early flowering (Goto et a ...
... flowering under LDs and reduced FT levels (Johnson et al., 1994; Guo et al., 1998; Yanovsky and Kay, 2002). Particularly, loss of CRY2 function delays flowering without affecting the diurnal pattern of CO mRNA (Yanovsky and Kay, 2002). In contrast, phyB mutations result in early flowering (Goto et a ...
Bacterial tail anchors can target to the mitochondrial outer
... mitochondrial OM that would allow full integration of mitochondria within the ancestral ...
... mitochondrial OM that would allow full integration of mitochondria within the ancestral ...
Centromeres: An Integrated Protein/DNA Complex
... elements dg (3.8 kb), dh (4.0 kb), and yn/tm (less than 1 kb) (Nakaseko al 1986; Niwa et al 1989; Chikashige et al 1989; Matsumotoet al 1990). The dh region corresponds to a portion of K-L; dg to K, and yn/tm to B, respectively. Eachof these elements, or portions thereof, occur at all three S. pombe ...
... elements dg (3.8 kb), dh (4.0 kb), and yn/tm (less than 1 kb) (Nakaseko al 1986; Niwa et al 1989; Chikashige et al 1989; Matsumotoet al 1990). The dh region corresponds to a portion of K-L; dg to K, and yn/tm to B, respectively. Eachof these elements, or portions thereof, occur at all three S. pombe ...
Theoretical studies of Membrane Proteins
... and 3D structures. Prediction of the membrane spanning regions by hydrophobicity analysis is a key technique used in several of the studies. A novel method for this is also presented and compared to other methods. The primary questions addressed in the thesis are: What properties are common to all m ...
... and 3D structures. Prediction of the membrane spanning regions by hydrophobicity analysis is a key technique used in several of the studies. A novel method for this is also presented and compared to other methods. The primary questions addressed in the thesis are: What properties are common to all m ...
11. Kikkawa, M. et al. Switch-based mechanism of
... localize mRNAs encoding a subset of myelin proteins, including MBP and 36K. To determine if kif1b is required for the formation of myelin in CNS, we analyzed ultrastructure of myelinated axons by TEM. The number of myelinated axons was reduced at 9 dpf in kif1bst43 mutants in both the anterior hindb ...
... localize mRNAs encoding a subset of myelin proteins, including MBP and 36K. To determine if kif1b is required for the formation of myelin in CNS, we analyzed ultrastructure of myelinated axons by TEM. The number of myelinated axons was reduced at 9 dpf in kif1bst43 mutants in both the anterior hindb ...
Lecture 7 Notes CH.7
... structure of the plasma membrane • 7.1 Cellular membranes are fluid mosaics of lipids and proteins Descriptive term regarding the • 7.2 Membrane structure results in selective permeability ...
... structure of the plasma membrane • 7.1 Cellular membranes are fluid mosaics of lipids and proteins Descriptive term regarding the • 7.2 Membrane structure results in selective permeability ...
Enzymes: “Helper” Protein molecules
... Each enzyme is the specific helper to a specific reaction each enzyme needs to be the right shape for the job enzymes are named for the reaction they help ...
... Each enzyme is the specific helper to a specific reaction each enzyme needs to be the right shape for the job enzymes are named for the reaction they help ...
PcGs and Hox genes - Development
... that it occurs within a few cell generations of the loss of repression. These results suggest a functional distinction between transcriptional repression and heritable silencing: in at least some contexts, Hox genes can retain the capacity to be heritably silenced, despite being transcribed and repl ...
... that it occurs within a few cell generations of the loss of repression. These results suggest a functional distinction between transcriptional repression and heritable silencing: in at least some contexts, Hox genes can retain the capacity to be heritably silenced, despite being transcribed and repl ...
Vertebrate Hedgehog signalling modulated by induction of a
... with that of zebra®sh, chick and human (data not shown) indicates that the Xenopus Gene 5 is not the Xenopus Hip orthologue. The ®nding that Hip can cause Hedgehog::AP fusion proteins to be retained on the cell surface and the presence of a putative signal peptide and transmembrane domain strongly s ...
... with that of zebra®sh, chick and human (data not shown) indicates that the Xenopus Gene 5 is not the Xenopus Hip orthologue. The ®nding that Hip can cause Hedgehog::AP fusion proteins to be retained on the cell surface and the presence of a putative signal peptide and transmembrane domain strongly s ...
Repositório da Universidade de Lisboa
... 2.3 A) Flowchart of the F motif-based sequence confirmation; B) ROC curve for the validation of this approach. Each dot represents the average of a cross-validation. The different colours indicate the threshold level used. As can be seen, a perfect true positive rate is achieved with two motifs dete ...
... 2.3 A) Flowchart of the F motif-based sequence confirmation; B) ROC curve for the validation of this approach. Each dot represents the average of a cross-validation. The different colours indicate the threshold level used. As can be seen, a perfect true positive rate is achieved with two motifs dete ...
MEMBRANE PROTEINS SYNTHESIZED BY
... the isolated enzyme forms stable interactions with high affinity for a limited number of sites on the cytoplasmic face of the membrane (21). Rabbit reticulocytes contain, with possibly one or two exceptions (22), the same proteins in their membranes as do rabbit erythrocytes. We recently showed that ...
... the isolated enzyme forms stable interactions with high affinity for a limited number of sites on the cytoplasmic face of the membrane (21). Rabbit reticulocytes contain, with possibly one or two exceptions (22), the same proteins in their membranes as do rabbit erythrocytes. We recently showed that ...
- Mochtar Riady Institute
... note that they were still functional in regulating MDDC function similarly to the fulllength AFP derived from purified human cord blood serum, which presumably are glycosylated. These results indicated that glycosylation of AFP seems to be unnecessary in their immunoregulatory function. Our result ...
... note that they were still functional in regulating MDDC function similarly to the fulllength AFP derived from purified human cord blood serum, which presumably are glycosylated. These results indicated that glycosylation of AFP seems to be unnecessary in their immunoregulatory function. Our result ...
view file - S
... Early analysis indicated that SGR1 might function in chlorophyll (Chl) degradation, as: (i) sgr1 knockout mutants exhibit a common stay-green phenotype in several plant species, (ii) sgr1 mutants display a type C nonfunctional (cosmetic) staygreen phenotype, which affects Chl degradation, but not ot ...
... Early analysis indicated that SGR1 might function in chlorophyll (Chl) degradation, as: (i) sgr1 knockout mutants exhibit a common stay-green phenotype in several plant species, (ii) sgr1 mutants display a type C nonfunctional (cosmetic) staygreen phenotype, which affects Chl degradation, but not ot ...
Ribosomopathies: human disorders of ribosome
... dyskerin, which adds specific DNA sequence repeats to the ends of chromosomes and counters some of the normal shortening that occurs during DNA replication.52 X-linked DKC, which has a more severe phenotype compared with the autosomal dominant form of DKC, is caused by a mutation in DKC1, which enco ...
... dyskerin, which adds specific DNA sequence repeats to the ends of chromosomes and counters some of the normal shortening that occurs during DNA replication.52 X-linked DKC, which has a more severe phenotype compared with the autosomal dominant form of DKC, is caused by a mutation in DKC1, which enco ...
ATP-binding-cassette (ABC) transport systems: Functional and
... by two short sequence motifs in their primary structure (`Walker' site A: GXXGXGKS/T, X can be varied; `Walker' site B: hhhhD, h stands for hydrophobic) that are supposed to constitute a nucleotide binding fold [7,8]. The Walker B-site is immediately preceded by a highly conserved sequence motif (`l ...
... by two short sequence motifs in their primary structure (`Walker' site A: GXXGXGKS/T, X can be varied; `Walker' site B: hhhhD, h stands for hydrophobic) that are supposed to constitute a nucleotide binding fold [7,8]. The Walker B-site is immediately preceded by a highly conserved sequence motif (`l ...
Conservation of inner nuclear membrane targeting sequences in
... nal domain at the N-terminus and a large C-terminal extralumenal domain. The NLS region of Pom121, containing four or five boxes of basic residues, is highly conserved among species and encodes at least two bipartite NLSs (Yavuz et al., 2010; Funakoshi et al., 2011). The region between the TM domain ...
... nal domain at the N-terminus and a large C-terminal extralumenal domain. The NLS region of Pom121, containing four or five boxes of basic residues, is highly conserved among species and encodes at least two bipartite NLSs (Yavuz et al., 2010; Funakoshi et al., 2011). The region between the TM domain ...
The Gene Gateway Workbook
... instructions designed to introduce new users to genetic-disorder and bioinformatics resources freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site ...
... instructions designed to introduce new users to genetic-disorder and bioinformatics resources freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site ...
Ribosomopathies: human disorders of ribosome
... dyskerin, which adds specific DNA sequence repeats to the ends of chromosomes and counters some of the normal shortening that occurs during DNA replication.52 X-linked DKC, which has a more severe phenotype compared with the autosomal dominant form of DKC, is caused by a mutation in DKC1, which enco ...
... dyskerin, which adds specific DNA sequence repeats to the ends of chromosomes and counters some of the normal shortening that occurs during DNA replication.52 X-linked DKC, which has a more severe phenotype compared with the autosomal dominant form of DKC, is caused by a mutation in DKC1, which enco ...
blumberg-lab.bio.uci.edu
... ● Is replicated first and has most origins of replication ● Concentrates nucleosome remodeling ● Contains key protein in chromosome structure ● Genes are linked to behavior and tissue specific processes ...
... ● Is replicated first and has most origins of replication ● Concentrates nucleosome remodeling ● Contains key protein in chromosome structure ● Genes are linked to behavior and tissue specific processes ...
Chromatin: A sticky silence
... The silent mating-type (HM) loci and telomeres of the budding yeast, Saccharomyces cerevisiae, are considered to be heterochromatin-like regions, because they confer a generalized transcriptional repression, which at telomeres occurs in a variegated or stochastic fashion. The repression is mediated ...
... The silent mating-type (HM) loci and telomeres of the budding yeast, Saccharomyces cerevisiae, are considered to be heterochromatin-like regions, because they confer a generalized transcriptional repression, which at telomeres occurs in a variegated or stochastic fashion. The repression is mediated ...
Session 238 Lens development and molecular cell biology
... elongation in the AQP0-KO lens fiber cells. The presence of elongated, disorganized BSs was confirmed by SEM. Freeze-fracture immunogold TEM showed that GJ particles were greatly dispersed in the elongated BSs. For the identity and distinct patterns of GJ-BSs on the broad-surface of superficial fibe ...
... elongation in the AQP0-KO lens fiber cells. The presence of elongated, disorganized BSs was confirmed by SEM. Freeze-fracture immunogold TEM showed that GJ particles were greatly dispersed in the elongated BSs. For the identity and distinct patterns of GJ-BSs on the broad-surface of superficial fibe ...
Newly Synthesized Catalytic and Regulatory Components of
... a turnover number of 2000 mini, cell bodies and neurites were calculated to contain similar densities of catalytic unit molecules on their surface (9-28 molecules/ pm2). An abundant GTP binding protein, detected by ADPribosylation with pertussis toxin, was also widely distributed in the neuron. A co ...
... a turnover number of 2000 mini, cell bodies and neurites were calculated to contain similar densities of catalytic unit molecules on their surface (9-28 molecules/ pm2). An abundant GTP binding protein, detected by ADPribosylation with pertussis toxin, was also widely distributed in the neuron. A co ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.