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O A RIGINAL RTICLE
... Bacterial enzymes are the ones that are having wide variety of industrial applications. Among these enzymes, a-amylase having high demand in industries such as pharmaceuticals, textiles, food processing etc; So, in this study we have identified novel Bacillus cereus MS6 bacteria, which can produces ...
... Bacterial enzymes are the ones that are having wide variety of industrial applications. Among these enzymes, a-amylase having high demand in industries such as pharmaceuticals, textiles, food processing etc; So, in this study we have identified novel Bacillus cereus MS6 bacteria, which can produces ...
The SR Protein SRp38 Represses Splicing in M Phase Cells
... Among those uncovered was a protein with strong sequence similarity to SR proteins. Two forms of this protein, likely arising from alternative splicing, were recovered. Strengthening the similarity with SR proteins, the RBD showed the highest homology with that of the classical SR protein SC35 (46% ...
... Among those uncovered was a protein with strong sequence similarity to SR proteins. Two forms of this protein, likely arising from alternative splicing, were recovered. Strengthening the similarity with SR proteins, the RBD showed the highest homology with that of the classical SR protein SC35 (46% ...
Small-molecule binding sites to explore new targets in the cancer
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
Gene Name
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
Plant Two-Component Signaling Systems and the Role of Response
... a putative hybrid kinase (CKI1) was described by Kakimoto (1996), which, when overexpressed, resulted in cytokinin-independent growth of calli. However, most of the important advances came just recently. By screening for the loss of cytokinindependent greening and shoot formation of hypocotyl explan ...
... a putative hybrid kinase (CKI1) was described by Kakimoto (1996), which, when overexpressed, resulted in cytokinin-independent growth of calli. However, most of the important advances came just recently. By screening for the loss of cytokinindependent greening and shoot formation of hypocotyl explan ...
Host cytoskeleton in respiratory syncytial virus assembly and
... strains are highly attenuated in vivo [19]. Glycosaminoglycans (GAGs) are cell surface receptors implicated in RSV attachment in cell culture, as well for many other viruses [20, 21]. Interestingly, nucleolin has been shown ...
... strains are highly attenuated in vivo [19]. Glycosaminoglycans (GAGs) are cell surface receptors implicated in RSV attachment in cell culture, as well for many other viruses [20, 21]. Interestingly, nucleolin has been shown ...
Ubiquitination and Auxin Signaling: A Degrading Story
... was not the case (Abel et al., 1994), recent work examining the stability of Aux/IAA-reporter fusion proteins expressed from non-auxin-responsive promoters and using different time frames has reached different conclusions. These studies were able to uncouple any auxin-induced Aux/IAA protein destabi ...
... was not the case (Abel et al., 1994), recent work examining the stability of Aux/IAA-reporter fusion proteins expressed from non-auxin-responsive promoters and using different time frames has reached different conclusions. These studies were able to uncouple any auxin-induced Aux/IAA protein destabi ...
Identification of a novel gene family involved in osmotic
... rescued the osm-7 phenotype completely. RNAi of each of the five genes on T05D4 revealed that knockdown of only one gene, T05D4.4, produced a slow-growing, Dec phenotype. In addition, we found a nonsense mutation in the coding sequence of T05D4.4 in the osm-7(sa89) strain (Figure 2b). Together thes ...
... rescued the osm-7 phenotype completely. RNAi of each of the five genes on T05D4 revealed that knockdown of only one gene, T05D4.4, produced a slow-growing, Dec phenotype. In addition, we found a nonsense mutation in the coding sequence of T05D4.4 in the osm-7(sa89) strain (Figure 2b). Together thes ...
Keratin Alterations during Embryonic Epidermal Differentiation: A
... protein (labeled a in Fig. 2) appeared and paralleled the commitment of the cells to terminal differentiation . The 64and 61-kdalton keratins (labeled b and c, respectively) were not observed until 29 d of embryonic life when the most obvious morphological change in the epidermis was the development ...
... protein (labeled a in Fig. 2) appeared and paralleled the commitment of the cells to terminal differentiation . The 64and 61-kdalton keratins (labeled b and c, respectively) were not observed until 29 d of embryonic life when the most obvious morphological change in the epidermis was the development ...
Basic region of residues 228-231 of protein kinase CK1[alpha] is
... ly diminish their capacity to bind axin (not shown). Effect of Axin Binding on CK1a Activity The question of whether the binding of CK1a to GST-axin had any effect on the enzymatic activity of the kinase was addressed by several experiments. The apparent Km values of CK1a for three substrates that d ...
... ly diminish their capacity to bind axin (not shown). Effect of Axin Binding on CK1a Activity The question of whether the binding of CK1a to GST-axin had any effect on the enzymatic activity of the kinase was addressed by several experiments. The apparent Km values of CK1a for three substrates that d ...
A conserved blueprint for the eye? - treisman lab
... been found in the eyes of many other species, including those with very primitive eyes.(37–41) Thus, despite the striking structural and developmental differences between the insect compound eye and the vertebrate single-lens eye, it has been suggested that they both evolved from a common precursor ...
... been found in the eyes of many other species, including those with very primitive eyes.(37–41) Thus, despite the striking structural and developmental differences between the insect compound eye and the vertebrate single-lens eye, it has been suggested that they both evolved from a common precursor ...
Ageing and the aggregating proteoglycans of
... a central core protein to which one or more glycosaminoglycan chains are covalently attached. The glycosaminoglycan may be chondroitin sulphate, dermatan sulphate, keratan sulphate or heparan sulphate, depending on the tissue in question. In the fibrous connective tissues, such as skin, ligament and ...
... a central core protein to which one or more glycosaminoglycan chains are covalently attached. The glycosaminoglycan may be chondroitin sulphate, dermatan sulphate, keratan sulphate or heparan sulphate, depending on the tissue in question. In the fibrous connective tissues, such as skin, ligament and ...
Fluorescent Reporter Proteins for the Tonoplast
... due the propensity of GFP itself to travel to vacuoles (Frigerio et al., 2001a; Zheng et al., 2004) and its lack of fluorescence in the acidic vacuolar lumen (Fluckiger et al., 2003; Tamura et al., 2003; Samalova et al., 2006). Regarding the distribution of TIPs in Arabidopsis, the available evidenc ...
... due the propensity of GFP itself to travel to vacuoles (Frigerio et al., 2001a; Zheng et al., 2004) and its lack of fluorescence in the acidic vacuolar lumen (Fluckiger et al., 2003; Tamura et al., 2003; Samalova et al., 2006). Regarding the distribution of TIPs in Arabidopsis, the available evidenc ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... the Ca2+-stimulation of the photoprotein aequorin8. The blue light generated by aequorin excites GFP and results in the emission of green light. GFP itself consists of 238 amino acids (Mr = 27 kDa) and is synthesized as an apoprotein in which post-translational formation of the chromophore occurs in ...
... the Ca2+-stimulation of the photoprotein aequorin8. The blue light generated by aequorin excites GFP and results in the emission of green light. GFP itself consists of 238 amino acids (Mr = 27 kDa) and is synthesized as an apoprotein in which post-translational formation of the chromophore occurs in ...
Isolation of a New High Molecular Weight Protein Associated with
... vitro and exhibit indistinguishable spatial distributions (7, 11) . In early myogenesis they are associated with cytoplasmic filaments and in later myogenesis they become associated with myofibril Z disks (7, 11) . In adult skeletal myofibrils, these three proteins are localized at the Z disk and in ...
... vitro and exhibit indistinguishable spatial distributions (7, 11) . In early myogenesis they are associated with cytoplasmic filaments and in later myogenesis they become associated with myofibril Z disks (7, 11) . In adult skeletal myofibrils, these three proteins are localized at the Z disk and in ...
Macaya Whole STUDENT`S WORKSHEETS
... have two X chromosome (XX) only will have the disease when they have the gene in both (rare). The probability of a woman having the disease is very low, though possible. That would happen if both father and mother had the affected haemophilic gene. Frequently female are only carriers because this ge ...
... have two X chromosome (XX) only will have the disease when they have the gene in both (rare). The probability of a woman having the disease is very low, though possible. That would happen if both father and mother had the affected haemophilic gene. Frequently female are only carriers because this ge ...
Temperature-Sensitive Mutations Made Easy: Generating
... OSS-OF-FUNCTION phenotypes provide critical insights into gene functions. Conventional genetargeting techniques generate loss-of-function mutations by permanently deleting the specific gene of interest or by rendering it nonfunctional. However, this strategy falls short for two groups of genes: esse ...
... OSS-OF-FUNCTION phenotypes provide critical insights into gene functions. Conventional genetargeting techniques generate loss-of-function mutations by permanently deleting the specific gene of interest or by rendering it nonfunctional. However, this strategy falls short for two groups of genes: esse ...
Phosphorylation of Beclin 1 by DAP
... DAPk, a calcium/calmodulin Ser/Thr kinase, functions as a tumor suppressor gene and has been linked to several cell death pathways, including autophagic cell death. Using pull-down assays, as well as immunoprecipitation experiments, we show that DAPk physically interacts with Beclin 1. Interestingly ...
... DAPk, a calcium/calmodulin Ser/Thr kinase, functions as a tumor suppressor gene and has been linked to several cell death pathways, including autophagic cell death. Using pull-down assays, as well as immunoprecipitation experiments, we show that DAPk physically interacts with Beclin 1. Interestingly ...
Developmentally regulated, alternative splicing of the Rpn10 gene
... (Figure 3A). However, the fragments of ~520 and ~870 bp were ampli®ed only from embryonic brain and embryonic stem cell cDNA libraries, respectively. Partial sequence analysis of these fragments showed that the ~870, ~520 and ~370 bp bands correspond to the Rpn10c, Rpn10e and Rpn10a mRNAs, respectiv ...
... (Figure 3A). However, the fragments of ~520 and ~870 bp were ampli®ed only from embryonic brain and embryonic stem cell cDNA libraries, respectively. Partial sequence analysis of these fragments showed that the ~870, ~520 and ~370 bp bands correspond to the Rpn10c, Rpn10e and Rpn10a mRNAs, respectiv ...
Site-selective incorporation and ligation of
... when using this method however as other amino acids can be oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess methionine or ethylene glycol, followed by purification o ...
... when using this method however as other amino acids can be oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess methionine or ethylene glycol, followed by purification o ...
PDF - Iqbal Hamza
... yeast, but a chimeric molecule consisting of the aminoterminal domain of yeast sco1 and the carboxyl-terminal domain of human sco1 containing the CXXXC motif will correct the respiratory incompetence in this mutant strain (Paret et al., 1999). Using this approach, the P147L patient mutation was foun ...
... yeast, but a chimeric molecule consisting of the aminoterminal domain of yeast sco1 and the carboxyl-terminal domain of human sco1 containing the CXXXC motif will correct the respiratory incompetence in this mutant strain (Paret et al., 1999). Using this approach, the P147L patient mutation was foun ...
How Insulin Works
... "something" will require perception of interplay between all of the many factors involved in the endocrinologic control of metabolism. We are still a long way from a good understanding of the mechanisms through which insulin controls metabolism. However, this is an extremely active research area and ...
... "something" will require perception of interplay between all of the many factors involved in the endocrinologic control of metabolism. We are still a long way from a good understanding of the mechanisms through which insulin controls metabolism. However, this is an extremely active research area and ...
Characterization of the Distal Polyadenylation Site of the ß
... Most genes have multiple polyadenylation sites (PAS), which are often selected in a tissue-specific manner, altering protein products and affecting mRNA stability, subcellular localization and/or translability. Here we studied the polyadenylation mechanisms associated to the beta-adducin gene (Add2) ...
... Most genes have multiple polyadenylation sites (PAS), which are often selected in a tissue-specific manner, altering protein products and affecting mRNA stability, subcellular localization and/or translability. Here we studied the polyadenylation mechanisms associated to the beta-adducin gene (Add2) ...
Review Article RNA-Binding Proteins in Amyotrophic Lateral
... Most of the TDP-43 ALS-linked mutations tested to date accelerate aggregation in vitro, whereas the FUS mutations have no effect [21, 22]. Q331K and M337V, for instance, are particularly aggressive TDP-43 variants that aggregate more rapidly than WT [21]. The FUS mutants H517Q, R521C, and R521H do no ...
... Most of the TDP-43 ALS-linked mutations tested to date accelerate aggregation in vitro, whereas the FUS mutations have no effect [21, 22]. Q331K and M337V, for instance, are particularly aggressive TDP-43 variants that aggregate more rapidly than WT [21]. The FUS mutants H517Q, R521C, and R521H do no ...
The location and type of mutation predict
... intellectual disability only (3). Lissencephaly is one component of the contiguous gene deletion disorder known as Miller– Dieker syndrome (MDS) or may occur as an isolated brain malformation in patients with isolated lissencephaly sequence (ILS) (2). The clinical severity generally correlates with ...
... intellectual disability only (3). Lissencephaly is one component of the contiguous gene deletion disorder known as Miller– Dieker syndrome (MDS) or may occur as an isolated brain malformation in patients with isolated lissencephaly sequence (ILS) (2). The clinical severity generally correlates with ...
Protein moonlighting
![](https://commons.wikimedia.org/wiki/Special:FilePath/3EL3.png?width=300)
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.