Escherichia coli Karl Skoog
... this protein can bundle FtsZ protofilaments, as it could bridge two protofilaments. Penicillin-binding protein 5 (PBP5) has been found to localize to the septum and it has been suggested to be connected to cell division. I have found that PBP5 forms a homo-oligomeric complex, most likely a dimer. Th ...
... this protein can bundle FtsZ protofilaments, as it could bridge two protofilaments. Penicillin-binding protein 5 (PBP5) has been found to localize to the septum and it has been suggested to be connected to cell division. I have found that PBP5 forms a homo-oligomeric complex, most likely a dimer. Th ...
The Plastidic Phosphoglucomutase from
... phenotype with only 1% of the seed dry weight as starch, compared with 60% in the wild type (Harrison et al., 1998). The reduction on starch synthesis in rug3 is also accompanied by a significant increase in seed lipid content, and a decreased legumin to vicilian ratio (Casey et al., 1998). Both the ...
... phenotype with only 1% of the seed dry weight as starch, compared with 60% in the wild type (Harrison et al., 1998). The reduction on starch synthesis in rug3 is also accompanied by a significant increase in seed lipid content, and a decreased legumin to vicilian ratio (Casey et al., 1998). Both the ...
enterocolitica Yersinia Type III Secretion System of Properties of the
... and trifluoroacetic acid (TFA). One volume of cell culture was mixed with 1 volume of 10% TCA, and the mixture was incubated for 20 min on ice. Insoluble material was collected by centrifugation at 20,000 ⫻ g for 20 min at 4°C, washed twice with 2 volumes of ice-cold acetone, and dried. To increase ...
... and trifluoroacetic acid (TFA). One volume of cell culture was mixed with 1 volume of 10% TCA, and the mixture was incubated for 20 min on ice. Insoluble material was collected by centrifugation at 20,000 ⫻ g for 20 min at 4°C, washed twice with 2 volumes of ice-cold acetone, and dried. To increase ...
A plant dialect of the histone language
... www.sciencedirect.com 1360-1385/$ - see front matter q 2003 Elsevier Ltd. All rights reserved. doi:10.1016/j.tplants.2003.12.007 ...
... www.sciencedirect.com 1360-1385/$ - see front matter q 2003 Elsevier Ltd. All rights reserved. doi:10.1016/j.tplants.2003.12.007 ...
Homologous recombination in budding yeast expressing the human
... expression was controlled by the ADH1 promoter and terminator sequences. Western blots of whole cell extracts from strains containing the adh1::HsRAD52-FLAG allele displayed a 49 kDa peptide consistent with that expected for HsRAD52-FLAG while extracts from strains containing the RAD52-FLAG allele y ...
... expression was controlled by the ADH1 promoter and terminator sequences. Western blots of whole cell extracts from strains containing the adh1::HsRAD52-FLAG allele displayed a 49 kDa peptide consistent with that expected for HsRAD52-FLAG while extracts from strains containing the RAD52-FLAG allele y ...
COST Functional Modeling Workshop
... • is used for a diverse range of species • structured to be queried at different levels, eg: • find all the chicken gene products in the genome that are involved in signal transduction • zoom in on all the receptor tyrosine kinases ...
... • is used for a diverse range of species • structured to be queried at different levels, eg: • find all the chicken gene products in the genome that are involved in signal transduction • zoom in on all the receptor tyrosine kinases ...
Emergence of robust growth laws from optimal regulation of
... amino acids, nucleosides and vitamin supplements), the doubling time can be easily varied from 20 min up to several hours. Under these conditions, with growth rate modulated by nutrient quality, the ribosomal protein fraction increases linearly with the growth rate (black line, Fig 1A). Conversely, ...
... amino acids, nucleosides and vitamin supplements), the doubling time can be easily varied from 20 min up to several hours. Under these conditions, with growth rate modulated by nutrient quality, the ribosomal protein fraction increases linearly with the growth rate (black line, Fig 1A). Conversely, ...
Filip Jagodzinski - WWU Computer Science Faculty Web Pages
... Pass Points on High Dimensional Surfaces, 2013 Symposium on University Research and Creative Expression (SOURCE), Central Washington University. Brian Orndorff*, Filip Jagodzinski, Rigidity Analysis of Protein-Ligand Conformations Generated by Molecular Dynamics, 2013 Symposium on University Researc ...
... Pass Points on High Dimensional Surfaces, 2013 Symposium on University Research and Creative Expression (SOURCE), Central Washington University. Brian Orndorff*, Filip Jagodzinski, Rigidity Analysis of Protein-Ligand Conformations Generated by Molecular Dynamics, 2013 Symposium on University Researc ...
Protein Creation Pathway
... Correct! That was the nucleolus. I hope this tutorial was helpful. This will be posted on my website for you to review at any time. Turn in your handout. Feel free to start over if you would like to do some review. ...
... Correct! That was the nucleolus. I hope this tutorial was helpful. This will be posted on my website for you to review at any time. Turn in your handout. Feel free to start over if you would like to do some review. ...
Comparison of the activities of protein disulphide
... specific activity of PDI was 25-fold greater than that of thioredoxin. 3. The greater efficiency of PDI compared with thioredoxin is considered to be due more to the presence of multiple catalytic domains in PDI than to differences in their active-site sequences. 4. Data and procedures were defined ...
... specific activity of PDI was 25-fold greater than that of thioredoxin. 3. The greater efficiency of PDI compared with thioredoxin is considered to be due more to the presence of multiple catalytic domains in PDI than to differences in their active-site sequences. 4. Data and procedures were defined ...
The unique proline-rich domain of parotid proline
... The cDNA for tG was a gift from Dr J. Rose and was cloned into the pLEN expression vector (Neufeld et al., 1987). The signal and propeptide sequences of preproPTH with added 5′ SalI and 3′ HindIII sites were synthesized by PCR, inserted into pBluescript, and the primary structure was confirmed by DN ...
... The cDNA for tG was a gift from Dr J. Rose and was cloned into the pLEN expression vector (Neufeld et al., 1987). The signal and propeptide sequences of preproPTH with added 5′ SalI and 3′ HindIII sites were synthesized by PCR, inserted into pBluescript, and the primary structure was confirmed by DN ...
Functional Roles Of Lipids In membranes - IJS
... ionic interaction with water and therefore are energetically stable in an aqueous environment. The structural organization that a polar lipid assumes in water is determined by its concentration and the law of opposing forces, i.e. hydrophobic forces driving self-association versus steric and ionic ...
... ionic interaction with water and therefore are energetically stable in an aqueous environment. The structural organization that a polar lipid assumes in water is determined by its concentration and the law of opposing forces, i.e. hydrophobic forces driving self-association versus steric and ionic ...
Evolution of Mammalian KELL Blood Group Glycoproteins and
... proteins are shown in Figure 1, together with previously reported sequences for human [2] and mouse KELL proteins [9] (Table 1). Alignments of human with other mammalian sequences examined were between 55-98% identical, suggesting that these are members of the same gene family, whereas comparisons o ...
... proteins are shown in Figure 1, together with previously reported sequences for human [2] and mouse KELL proteins [9] (Table 1). Alignments of human with other mammalian sequences examined were between 55-98% identical, suggesting that these are members of the same gene family, whereas comparisons o ...
ANALYSIS OF THE BACTERIOPHAGE P22 VIRAL SPREAD CHARACTERIZATION AS IMMUNOBIOSENSOR
... 2. Latent Period (time around 20-25 min between adsorption and the lysis). Replication of the virus genome occurs within the bacterial cell, without increase in extracellular phage (Doermann, A.H., 1952). 2.1. Eclipse period: It begins after the nucleic acid is injected and finishes when the first p ...
... 2. Latent Period (time around 20-25 min between adsorption and the lysis). Replication of the virus genome occurs within the bacterial cell, without increase in extracellular phage (Doermann, A.H., 1952). 2.1. Eclipse period: It begins after the nucleic acid is injected and finishes when the first p ...
A Calcium-Regulated Gatekeeper in Phloem Sieve Tubes
... (1991b) referred to “nondispersive” versus “dispersive” protein bodies, also called P-proteins or structural sieve element proteins. These terms arose because of changes that are observed during sieve element ontogeny. Early in the maturation process, many protein bodies are often observed, some of ...
... (1991b) referred to “nondispersive” versus “dispersive” protein bodies, also called P-proteins or structural sieve element proteins. These terms arose because of changes that are observed during sieve element ontogeny. Early in the maturation process, many protein bodies are often observed, some of ...
Localization of the mei-1 Gene Product of
... problem in spindle morphogenesis faced by the fertilized embryo: the same cytoplasm must support two very different types of division, often within a short time of one another. Parts of the regulatory network that confines mei-1 activity to the appropriate spindle may include the genes mei-2, mel26, ...
... problem in spindle morphogenesis faced by the fertilized embryo: the same cytoplasm must support two very different types of division, often within a short time of one another. Parts of the regulatory network that confines mei-1 activity to the appropriate spindle may include the genes mei-2, mel26, ...
Solubility of recombinant Src homology 2 domains expressed in E
... Background: Signalling proteins often contain several well defined and conserved protein domains. Structural analyses of such domains by nuclear magnetic spectroscopy or X-ray crystallography may greatly inform the function of proteins. A limiting step is often the production of sufficient amounts o ...
... Background: Signalling proteins often contain several well defined and conserved protein domains. Structural analyses of such domains by nuclear magnetic spectroscopy or X-ray crystallography may greatly inform the function of proteins. A limiting step is often the production of sufficient amounts o ...
A Directed Mutagenesis Screen in Drosophila
... of dpp transcriptional induction. Alternatively, it indicates that the variable suppression is due to the fact that ectopic dpp drives developmental changes in the posterior compartment (and hh using our driver does not), which might influence the observed phenotypes. Complementation and mapping ana ...
... of dpp transcriptional induction. Alternatively, it indicates that the variable suppression is due to the fact that ectopic dpp drives developmental changes in the posterior compartment (and hh using our driver does not), which might influence the observed phenotypes. Complementation and mapping ana ...
Disrupted mRNA sorting in CNS neurons
... and trans-acting proteins that confer stability, govern translation, and provide a means for movement. To date, most studies have focused on the sequences and structural features of mRNAs which are sufficient to direct localization when introduced as reporter constructs (Mayford et al., 1996b; MacDo ...
... and trans-acting proteins that confer stability, govern translation, and provide a means for movement. To date, most studies have focused on the sequences and structural features of mRNAs which are sufficient to direct localization when introduced as reporter constructs (Mayford et al., 1996b; MacDo ...
The Role of the Plant Nucleolus in Pre-mRNA Processing
... 2000; Olsen 2004; Raška et al. 2006; Boisvert et al. 2007). The multifunctional nature of the nucleolus has also been highlighted by proteomic approaches which have identified numerous unexpected proteins in the nucleolus, indicative of new interactions or functions (Andersen et al. 2002, 2005; Sche ...
... 2000; Olsen 2004; Raška et al. 2006; Boisvert et al. 2007). The multifunctional nature of the nucleolus has also been highlighted by proteomic approaches which have identified numerous unexpected proteins in the nucleolus, indicative of new interactions or functions (Andersen et al. 2002, 2005; Sche ...
Pontin and Reptin regulate cell proliferation in early Xenopus
... Initially, the homologues of Pontin and Reptin in human, rat and yeast (TIP49/TIP49a and TIP49b/TIP48) were characterized to interact with TATA-box binding protein (TBP) (Kanemaki et al, 1997; Kanemaki et al., 1999; Makino et al., 1999; Wood et al., 2000). They all share the conserved domains Walker ...
... Initially, the homologues of Pontin and Reptin in human, rat and yeast (TIP49/TIP49a and TIP49b/TIP48) were characterized to interact with TATA-box binding protein (TBP) (Kanemaki et al, 1997; Kanemaki et al., 1999; Makino et al., 1999; Wood et al., 2000). They all share the conserved domains Walker ...
effects of insulin and anchorage on hepatocytic protein metabolism
... tended to disappear when the total inhibition became large. The inhibition of protein degradation by insulin thus does not appear to require protein synthesis, but the hormone and the synthesis inhibitor seem to affect degradation by related mechanisms. The cycloheximide effect, like the insulin eff ...
... tended to disappear when the total inhibition became large. The inhibition of protein degradation by insulin thus does not appear to require protein synthesis, but the hormone and the synthesis inhibitor seem to affect degradation by related mechanisms. The cycloheximide effect, like the insulin eff ...
Identification of Genes Involved in the Assembly and Biosynthesis of... N
... Figure 1.2: Arrangements of flagella on a bacterial cell .................................................................. 7 Figure 1.3: Assembly of the bacterial flagellum .............................................................................. 9 Figure 1.4: Twitching motility using the Ty ...
... Figure 1.2: Arrangements of flagella on a bacterial cell .................................................................. 7 Figure 1.3: Assembly of the bacterial flagellum .............................................................................. 9 Figure 1.4: Twitching motility using the Ty ...
The p101 subunit of PI3Kγ restores activation by Gβ mutants
... enabling PtdIns(3,4,5)P3 formation by direct interaction of Gβγ with p110γ [17]. The underlying data suggest that the latter step is independent of p101; however, we could not exclude the possibility that p101 may be involved in Gβγ -induced stimulation of membrane-attached p110γ . Therefore Gβγ may ...
... enabling PtdIns(3,4,5)P3 formation by direct interaction of Gβγ with p110γ [17]. The underlying data suggest that the latter step is independent of p101; however, we could not exclude the possibility that p101 may be involved in Gβγ -induced stimulation of membrane-attached p110γ . Therefore Gβγ may ...
CHARACTERIZATION OF THE ROLE OF PSEUDOMONAS
... generous with his encouragement and support. Thank you to Ashutosh Tripathy of the UNC Macromolecular Interactions Facility, Brenda Temple of the UNC Center for Structural Biology, Lee Graves and John Sondek. Thank you to my committee for your patience as I developed as a scientist. Thanks to Miriam ...
... generous with his encouragement and support. Thank you to Ashutosh Tripathy of the UNC Macromolecular Interactions Facility, Brenda Temple of the UNC Center for Structural Biology, Lee Graves and John Sondek. Thank you to my committee for your patience as I developed as a scientist. Thanks to Miriam ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.