![Gene Section KLK4 (kallikrein-related peptidase 4) Atlas of Genetics and Cytogenetics](http://s1.studyres.com/store/data/008927684_1-1c9fd328c54f0a6040a72ebab8a42ba2-300x300.png)
Gene Section KLK4 (kallikrein-related peptidase 4) Atlas of Genetics and Cytogenetics
... both the mRNA and protein levels. For example, a tissue microarray study carried out on 42 benign and 207 malignant prostate tissues found that KLK4 was more highly expressed in prostate cancer cells when compared to benign cells, although one study using sandwich-type immunoassay on 16 malignant an ...
... both the mRNA and protein levels. For example, a tissue microarray study carried out on 42 benign and 207 malignant prostate tissues found that KLK4 was more highly expressed in prostate cancer cells when compared to benign cells, although one study using sandwich-type immunoassay on 16 malignant an ...
FluoProbes Luciferin substrates
... 1- Dissolve 1 mM luciferin or luciferin salt, 3 mM ATP, Triton-X 100 (1%), 25 mM glycylglycine, PH 7.8, 15 mM MgSO4 , 4 mm EGTA and 1 mM DTT in fresh desionized ATP free water Note: The Luciferin concentration can be checked by absorbance measurement at 385nm in 0.5 M carbonate buffer, pH 11.5. Mola ...
... 1- Dissolve 1 mM luciferin or luciferin salt, 3 mM ATP, Triton-X 100 (1%), 25 mM glycylglycine, PH 7.8, 15 mM MgSO4 , 4 mm EGTA and 1 mM DTT in fresh desionized ATP free water Note: The Luciferin concentration can be checked by absorbance measurement at 385nm in 0.5 M carbonate buffer, pH 11.5. Mola ...
Dr. Peter John M.Phil, PhD Assistant Professor
... anticodon that changes the codons to which it responds. When the new anticodon corresponds to a termination codon, an amino acid is inserted and the polypeptide chain is extended beyond the termination codon. This results in nonsense suppression at a site of nonsense mutation or in read through ...
... anticodon that changes the codons to which it responds. When the new anticodon corresponds to a termination codon, an amino acid is inserted and the polypeptide chain is extended beyond the termination codon. This results in nonsense suppression at a site of nonsense mutation or in read through ...
Evolution, structure and function of mitochondrial carriers: a
... subfamilies, the aspartate/glutamate and ATP-Mg/Pi carriers, have additional N-terminal regulatory domains (more than 150 amino acids), that usually contain Ca2+-binding motifs. Molecules transported by the MCF proteins are greatly variable in size and structure from H+ to NAD+ and coenzyme A. Most ...
... subfamilies, the aspartate/glutamate and ATP-Mg/Pi carriers, have additional N-terminal regulatory domains (more than 150 amino acids), that usually contain Ca2+-binding motifs. Molecules transported by the MCF proteins are greatly variable in size and structure from H+ to NAD+ and coenzyme A. Most ...
Presentation @3:30pm - Bioinformatics at School of Informatics
... •Targets for Future •References •Acknowledgements Translation is the process of synthesizing the peptide chain of amino acids specified by the nucleotide sequence on the mRNA. ...
... •Targets for Future •References •Acknowledgements Translation is the process of synthesizing the peptide chain of amino acids specified by the nucleotide sequence on the mRNA. ...
How do potentials derived from structural databases relate to true
... seen to occur much more often than others. About 17% of the conformations defined in the library are taken by 50% of the sequences as their lowest-energy conformation. However, because those sequences sharing a common conformation are not homologous to each other, they are not considered redundant s ...
... seen to occur much more often than others. About 17% of the conformations defined in the library are taken by 50% of the sequences as their lowest-energy conformation. However, because those sequences sharing a common conformation are not homologous to each other, they are not considered redundant s ...
Oxidative stress and disruption of labile iron generate specific
... requirements that indicate defects in iron homeostasis. The data in Table 2 show that strains with a lesion in one locus grew with wild-type proficiency in minimal medium. Growth of double mutant strains lacking gshA was compromised, with the yggX gshA mutant showing no detectable growth on minimal ...
... requirements that indicate defects in iron homeostasis. The data in Table 2 show that strains with a lesion in one locus grew with wild-type proficiency in minimal medium. Growth of double mutant strains lacking gshA was compromised, with the yggX gshA mutant showing no detectable growth on minimal ...
Etude Annotation
... score (525) than the GeneMark start at position 215 (420). The start at position 83 is yields the longest possible gene as well. Gap/Overlap: Since it is gene 1, we can omit determining the gap ...
... score (525) than the GeneMark start at position 215 (420). The start at position 83 is yields the longest possible gene as well. Gap/Overlap: Since it is gene 1, we can omit determining the gap ...
PDF
... Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but th ...
... Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but th ...
Charakterisierung peroxisomaler und Lipid
... proteins. PTS2 is usually located within the first 20 amino acids of the protein, and has been defined as (RK)-(LVIQ)-XX-(LVIHQ)-(LSGAK)-X-(HQ)-(LAF) (166). PTS2-bearing proteins are recognized by the cytosolic conserved receptor Pex7p (188). Based on the concept of cycling receptors (38, 144), the ...
... proteins. PTS2 is usually located within the first 20 amino acids of the protein, and has been defined as (RK)-(LVIQ)-XX-(LVIHQ)-(LSGAK)-X-(HQ)-(LAF) (166). PTS2-bearing proteins are recognized by the cytosolic conserved receptor Pex7p (188). Based on the concept of cycling receptors (38, 144), the ...
SERCA pump level is a critical determinant of Ca2+ homeostasis
... M. P S. H. SERCA Pump Level is a Critical Determinant of Ca2+ Homeostasis and Cardiac Contractility. Journal of Molecular and Cellular Cardiology (2001) 33, 1053–1063. The control of intracellular calcium is central to regulation of cardiac contractility. A defect in SR Ca2+ transport ...
... M. P S. H. SERCA Pump Level is a Critical Determinant of Ca2+ Homeostasis and Cardiac Contractility. Journal of Molecular and Cellular Cardiology (2001) 33, 1053–1063. The control of intracellular calcium is central to regulation of cardiac contractility. A defect in SR Ca2+ transport ...
Aminoacyl-tRNA synthetase complexes: beyond translation
... information from nucleic acids to proteins, they are thought to have emerged early in evolution and to be structurally highly tailored to specific recognition of substrate amino acids and tRNAs. Although the catalytic activities of these enzymes represent their essential role in maintenance of cell ...
... information from nucleic acids to proteins, they are thought to have emerged early in evolution and to be structurally highly tailored to specific recognition of substrate amino acids and tRNAs. Although the catalytic activities of these enzymes represent their essential role in maintenance of cell ...
Lanosterol Biosynthesis in the Prokaryote
... is the subject of our recent work after identifying the first proven prokaryotic sterol biosynthetic gene/protein (Jackson et al. 2002). The importance of sterols in eukaryotes is well established because they modulate membrane fluidity and also serve as precursor molecules for hormone and brassinos ...
... is the subject of our recent work after identifying the first proven prokaryotic sterol biosynthetic gene/protein (Jackson et al. 2002). The importance of sterols in eukaryotes is well established because they modulate membrane fluidity and also serve as precursor molecules for hormone and brassinos ...
Characterization of a Bacillus anthracis spore coat
... To assess general spore resistance properties, we subjected the Sterne strain cotb mutant (MGM68) to bleach, lysozyme, and heat challenges (using the methods described in Harwood & Cutting, 1990; Young & Setlow, 2003). We detected no significant differences between the mutant and wild-type strains ( ...
... To assess general spore resistance properties, we subjected the Sterne strain cotb mutant (MGM68) to bleach, lysozyme, and heat challenges (using the methods described in Harwood & Cutting, 1990; Young & Setlow, 2003). We detected no significant differences between the mutant and wild-type strains ( ...
PDF
... lens formation without the formation of the retina has been reported (Mencl, 1903; Spemann, 1912). How the lens develops in these species in the absence of the retina is not understood. The nature of this problem can best be demonstrated by the example of lens formation in the zebrafish retinal home ...
... lens formation without the formation of the retina has been reported (Mencl, 1903; Spemann, 1912). How the lens develops in these species in the absence of the retina is not understood. The nature of this problem can best be demonstrated by the example of lens formation in the zebrafish retinal home ...
ABSTRACT Title of Document:
... dimethylsulfoniopropionate (DMSP) catabolism (34), the major source of organic sulfur in the ocean (71). Dinoflagellates and other phytoplankton are primary producers of DMSP in the ocean (43, 117). The abundance and activity of Roseobacter species is significantly correlated with DMSP-producing din ...
... dimethylsulfoniopropionate (DMSP) catabolism (34), the major source of organic sulfur in the ocean (71). Dinoflagellates and other phytoplankton are primary producers of DMSP in the ocean (43, 117). The abundance and activity of Roseobacter species is significantly correlated with DMSP-producing din ...
Snake venomics of Bothrops punctatus
... was dissolved in 1 mL of water and, after stirring, the absorbance was measured at 228 nm. The percentage of ACE inhibition (% ACEi) was determined using the following formula; % ACEi = (Abs Control − Abs sample)/(Abs control − Abs blank). Control absorbance corresponded to hippuric acid formed afte ...
... was dissolved in 1 mL of water and, after stirring, the absorbance was measured at 228 nm. The percentage of ACE inhibition (% ACEi) was determined using the following formula; % ACEi = (Abs Control − Abs sample)/(Abs control − Abs blank). Control absorbance corresponded to hippuric acid formed afte ...
Vegetative incompatibility in filamentous fungi: Podospora and
... only 68% identical, which is extremely low for two allelic forms of the same locus [14•]. This is also true to a lesser extent for the products of the three het-C alleles of N. crassa. Overall, they display 86% identity, but identity drops to less than 25% in the region defining allele specificity [ ...
... only 68% identical, which is extremely low for two allelic forms of the same locus [14•]. This is also true to a lesser extent for the products of the three het-C alleles of N. crassa. Overall, they display 86% identity, but identity drops to less than 25% in the region defining allele specificity [ ...
Vaccinia Virus G1L Protein Expression and Purification
... ymxG (Hypothetical zinc protease from B. subtilis) ...
... ymxG (Hypothetical zinc protease from B. subtilis) ...
M6PRs are found in a subset of PC12 cell ISGs
... which contain hormones, are acidic, and have no clathrin patches. The protein composition of the ISG membrane is not well characterized, however the cation-independent (CI)mannose-6-phosphate receptor (M6PR), the cation-dependent (CD)-M6PR (Klumperman et al., 1998), and furin (Dittié et al., 1997) o ...
... which contain hormones, are acidic, and have no clathrin patches. The protein composition of the ISG membrane is not well characterized, however the cation-independent (CI)mannose-6-phosphate receptor (M6PR), the cation-dependent (CD)-M6PR (Klumperman et al., 1998), and furin (Dittié et al., 1997) o ...
Discovery and Characterization of a Novel Microtubule
... performed transcript profiling during wood development in poplar, a tree considered as a model for wood development. The majority of the genes upregulated during cellulose biosynthesis encode proteins with known or predictable functions, such as carbohydrate active enzymes (CAzymes). However, some o ...
... performed transcript profiling during wood development in poplar, a tree considered as a model for wood development. The majority of the genes upregulated during cellulose biosynthesis encode proteins with known or predictable functions, such as carbohydrate active enzymes (CAzymes). However, some o ...
insect transmitted plant pathogenic mollicutes, spiroplasma kunkelii
... localization study with the yellow fluorescent protein fusions of these two proteins revealed their localization in the plant nuclei and confirmed their dependence on plant importin α for nuclear transport. Transcripts corresponding to the phytoplasma proteins were detected in AY-WB phytoplasma-inf ...
... localization study with the yellow fluorescent protein fusions of these two proteins revealed their localization in the plant nuclei and confirmed their dependence on plant importin α for nuclear transport. Transcripts corresponding to the phytoplasma proteins were detected in AY-WB phytoplasma-inf ...
Effects of macromolecular crowding on protein folding and
... When oxidized lysozyme is refolded from 8 M urea in the presence of crowding agents, the yields of correctly folded protein are hardly affected. Identical results were obtained by refolding from 6 M guanidine HCl (GuHCl) as a denaturant (data not shown). Only at concentrations of crowding agents in ...
... When oxidized lysozyme is refolded from 8 M urea in the presence of crowding agents, the yields of correctly folded protein are hardly affected. Identical results were obtained by refolding from 6 M guanidine HCl (GuHCl) as a denaturant (data not shown). Only at concentrations of crowding agents in ...
PDF
... still growing, and not at the mid-pupal stage of development, when apoptosis removes supernumerary cells in the D. melanogaster eye. Therefore, we hypothesized that Ft and Ex control SWH pathway activity in the D. melanogaster eye in a temporally restricted fashion, i.e. during larval, but not pupal ...
... still growing, and not at the mid-pupal stage of development, when apoptosis removes supernumerary cells in the D. melanogaster eye. Therefore, we hypothesized that Ft and Ex control SWH pathway activity in the D. melanogaster eye in a temporally restricted fashion, i.e. during larval, but not pupal ...
PLANT COLD ACCLIMATION: Freezing Tolerance Genes and
... in the following section on Arabidopsis COR genes is that the amphipathic α-helical regions predicted to be present in many of the novel and LEA proteins may have roles in stabilizing membranes against freezing damage. Whether the regions predicted to form amphipathic α-helices actually form such st ...
... in the following section on Arabidopsis COR genes is that the amphipathic α-helical regions predicted to be present in many of the novel and LEA proteins may have roles in stabilizing membranes against freezing damage. Whether the regions predicted to form amphipathic α-helices actually form such st ...
Protein moonlighting
![](https://commons.wikimedia.org/wiki/Special:FilePath/3EL3.png?width=300)
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.