Biol115_2014_Lecture 10_Prokaryotic Gene Regulation
... Overview: conducting the genetic orchestra" • The genome contains all of the information needed for the growth and development of individuals." • Gene expression refers to the use of DNA sequences to synthesise RNA and proteins." • Some genes are expressed all the time (constitutive or housekeep ...
... Overview: conducting the genetic orchestra" • The genome contains all of the information needed for the growth and development of individuals." • Gene expression refers to the use of DNA sequences to synthesise RNA and proteins." • Some genes are expressed all the time (constitutive or housekeep ...
L16-Enzyme Structure
... Although only a small subset of the amino acids within an enzyme may engage the reactant(s), all enzyme constituents are needed for catalytic activity. Enormous molecule size generates: sufficient local-controlled flexibility precise three dimensional arrangements In spite of the tremendous stru ...
... Although only a small subset of the amino acids within an enzyme may engage the reactant(s), all enzyme constituents are needed for catalytic activity. Enormous molecule size generates: sufficient local-controlled flexibility precise three dimensional arrangements In spite of the tremendous stru ...
Fibrous proteins
... and hyper-extensibility of the joints and cardiovascular system. This syndrome is due to mutations in the gene for fibrilin. With this disease, abnormal fibrillin protein is incorporated into microfibrils along with normal fibrillin, inhibiting the formation of functional microfibrils. [Note: Patien ...
... and hyper-extensibility of the joints and cardiovascular system. This syndrome is due to mutations in the gene for fibrilin. With this disease, abnormal fibrillin protein is incorporated into microfibrils along with normal fibrillin, inhibiting the formation of functional microfibrils. [Note: Patien ...
The herpesvirus saimiri ORF73 gene product interacts with host
... for chromosome association of HVS ORF73 and this interaction requires the distal 123 aa. However, removal of 18 aa from the start or 12 aa from the end of this domain abolishes chromosomal association and we have described these domains as chromosome association sites (CAS) 1 and 2, respectively. Th ...
... for chromosome association of HVS ORF73 and this interaction requires the distal 123 aa. However, removal of 18 aa from the start or 12 aa from the end of this domain abolishes chromosomal association and we have described these domains as chromosome association sites (CAS) 1 and 2, respectively. Th ...
Ring, helix, sphere and cylinder: the basic geometry of prokaryotic
... co-transcription, translation and insertion of the membrane proteins into the membrane. A spatial bias would be generated as the replication of the chromosome proceeds, because of the transient nature of the constraints, the competition for membrane space and the cooperativity between neighbouring g ...
... co-transcription, translation and insertion of the membrane proteins into the membrane. A spatial bias would be generated as the replication of the chromosome proceeds, because of the transient nature of the constraints, the competition for membrane space and the cooperativity between neighbouring g ...
Gene Expression in C. elegans - Buffalo State College Faculty and
... Reporter genes can be used to show when and where a protein is expressed that implies the expression of a gene. The green fluorescent protein (GFP) reporter gene was cloned from jellyfish. The GFP gene can be fused to a piece of DNA and expressed with that gene. By observing the worm with the green ...
... Reporter genes can be used to show when and where a protein is expressed that implies the expression of a gene. The green fluorescent protein (GFP) reporter gene was cloned from jellyfish. The GFP gene can be fused to a piece of DNA and expressed with that gene. By observing the worm with the green ...
In Vitro Protein Expression Kit for Disulfide - Sigma
... and concentration of the template DNA are correct. If the purity of DNA is low, only a small amount of protein may be expressed. Re-purify the template DNA using commercially available purification kits. 3. F or high molecular weight protein genes and large circular DNA, the expression level may be ...
... and concentration of the template DNA are correct. If the purity of DNA is low, only a small amount of protein may be expressed. Re-purify the template DNA using commercially available purification kits. 3. F or high molecular weight protein genes and large circular DNA, the expression level may be ...
Isolation by Calcium-Dependent Translocation to
... membrane. These proteins were of molecular mass approximately 66, 40, 39, and 35 kD and they have been shown earlier to correspond to annexins.'" Binding to these proteins was to be expected because the cytosol antiserum contains antibodies against annexins I, 11, IV, and VI, as shown by ELISA using ...
... membrane. These proteins were of molecular mass approximately 66, 40, 39, and 35 kD and they have been shown earlier to correspond to annexins.'" Binding to these proteins was to be expected because the cytosol antiserum contains antibodies against annexins I, 11, IV, and VI, as shown by ELISA using ...
Highly conserved features of DNA binding between two divergent
... protein from S.cerevisiae (9). Proteins from this subfamily are involved in pre-mRNA splicing and there is no clear evidence yet that they can act as transcription factors (10). Single mutations of the tryptophan residues in the first or second repeat of Cef1p did not affect function of the protein ...
... protein from S.cerevisiae (9). Proteins from this subfamily are involved in pre-mRNA splicing and there is no clear evidence yet that they can act as transcription factors (10). Single mutations of the tryptophan residues in the first or second repeat of Cef1p did not affect function of the protein ...
Gene Section RASSF6 (Ras association (RalGDS/AF-6) domain family member 6)
... of RASSF6-induced apoptosis in a manner independent of the MST/Hippo tumour suppressor pathway. MOAP-1 regulates the 'extrinsic' pathway of apoptosis by acting downstream of death receptors such as the tumour necrosis factor a receptor 1 (TNF-R1) and TNFa apoptosis-inducing related ligand receptor 1 ...
... of RASSF6-induced apoptosis in a manner independent of the MST/Hippo tumour suppressor pathway. MOAP-1 regulates the 'extrinsic' pathway of apoptosis by acting downstream of death receptors such as the tumour necrosis factor a receptor 1 (TNF-R1) and TNFa apoptosis-inducing related ligand receptor 1 ...
Central Dogma of Molecular Biology: The way of diverting
... Table 1: Function of Proteins [2] Structure of Proteins [2] ...
... Table 1: Function of Proteins [2] Structure of Proteins [2] ...
novel 4E-interacting protein in Leishmania is involved in stage
... and subjected to western blot analysis with specific antibodies against the different LeishIF4F subunits. Mass spectrometry analysis Proteins that were pulled down with the tagged LeishIF4E-1 of LeishIF4E-4 from L. amazonensis cell lines (3–4 109 cells) were precipitated by TCA and further resolved ...
... and subjected to western blot analysis with specific antibodies against the different LeishIF4F subunits. Mass spectrometry analysis Proteins that were pulled down with the tagged LeishIF4E-1 of LeishIF4E-4 from L. amazonensis cell lines (3–4 109 cells) were precipitated by TCA and further resolved ...
Area 4: Molecular recognition in biomolecules Computational
... inhibitors. We plan to develop and implement a tool for the computational design of peptides binding to a protein surface. The method will be based on the recent observation that protein-peptide interactions adopt similar relative backbone arrangements as those observed between different regions of ...
... inhibitors. We plan to develop and implement a tool for the computational design of peptides binding to a protein surface. The method will be based on the recent observation that protein-peptide interactions adopt similar relative backbone arrangements as those observed between different regions of ...
Detection of plum pox potyviral protein–protein interactions in planta
... which are important for the assembly of particles. However, neither the core nor the C-terminal region was determined to be necessary for the physical self-interaction of the CPs in SMV-G7H (Kang et al., 2006) and PPV. This may indicate that the aforementioned amino acid motifs are not essential for ...
... which are important for the assembly of particles. However, neither the core nor the C-terminal region was determined to be necessary for the physical self-interaction of the CPs in SMV-G7H (Kang et al., 2006) and PPV. This may indicate that the aforementioned amino acid motifs are not essential for ...
File - Science at St. Dominics
... • Aligned amino acids bond to form links of the new protein molecule ...
... • Aligned amino acids bond to form links of the new protein molecule ...
The trans-Golgi network GRIP-domain proteins form α
... Members of the family of coiled-coil proteins specifically located on the Golgi (termed golgins), such as p115 and GM130, have been shown to be important as tethering molecules and in the biogenesis of membranes of the Golgi stack [1–5]. A number of golgins specifically associated with the TGN (tran ...
... Members of the family of coiled-coil proteins specifically located on the Golgi (termed golgins), such as p115 and GM130, have been shown to be important as tethering molecules and in the biogenesis of membranes of the Golgi stack [1–5]. A number of golgins specifically associated with the TGN (tran ...
Plant Hormone Signaling - Biol512
... The RNA-binding protein FCA is an abscisic acid receptor Razem FA, El-Kereamy A, Abrams SR, Hill RD. Department of Plant Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada Abstract The phytohormone abscisic acid (ABA) regulates various physiological processes in plants. The molecul ...
... The RNA-binding protein FCA is an abscisic acid receptor Razem FA, El-Kereamy A, Abrams SR, Hill RD. Department of Plant Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada Abstract The phytohormone abscisic acid (ABA) regulates various physiological processes in plants. The molecul ...
Mechanism - Blogs - University of Kent
... Function and explanation in protein synthesis • ‘In addition to the promoter-like elements, the Xenopus intergenic spacer contains repetitive short sequences, the 60/81 bp elements, that are clustered in tandem (Fig. 9.10) and which stimulate transcription when placed at a variable distance from th ...
... Function and explanation in protein synthesis • ‘In addition to the promoter-like elements, the Xenopus intergenic spacer contains repetitive short sequences, the 60/81 bp elements, that are clustered in tandem (Fig. 9.10) and which stimulate transcription when placed at a variable distance from th ...
Native and Artificial Reticuloplasmins Co
... with respect to seed storage proteins because of their nutritional importance for both plants and animals (for review, see Müntz, 1998). Furthermore, because the stable expression of heterologous proteins at high levels can depend on their accumulation in appropriate organelles, such studies may al ...
... with respect to seed storage proteins because of their nutritional importance for both plants and animals (for review, see Müntz, 1998). Furthermore, because the stable expression of heterologous proteins at high levels can depend on their accumulation in appropriate organelles, such studies may al ...
Cut, Print: Our Emerging Understanding of Alternative Splicing
... different proteins (Black 2000). Though only 49 of the Drosophila DSCAM exon combinations have been observed thus far, this example illustrates the sheer capacity for proteomic dynamism from a single alternatively spliced gene. ...
... different proteins (Black 2000). Though only 49 of the Drosophila DSCAM exon combinations have been observed thus far, this example illustrates the sheer capacity for proteomic dynamism from a single alternatively spliced gene. ...
Investigating the Role of RNA Polymerase II in RNAi
... al., 2009). These steps are not understood, and the interest of our proposal lies in elucidating RNAPII’s contribution in light of 3 mutations in subunits of the polymerase that compromise RNAi in different ways. RNAPII in S. pombe is a large protein, consisting of 12 different subunits (Spåhr, et a ...
... al., 2009). These steps are not understood, and the interest of our proposal lies in elucidating RNAPII’s contribution in light of 3 mutations in subunits of the polymerase that compromise RNAi in different ways. RNAPII in S. pombe is a large protein, consisting of 12 different subunits (Spåhr, et a ...
enzymes - 11biolcsc
... down a long protein into smaller chains called peptides . Peptidases break peptides down into individual amino acids. Proteases and peptidases are often found in laundry detergents -- they help remove things like blood stains from cloth by breaking down the ...
... down a long protein into smaller chains called peptides . Peptidases break peptides down into individual amino acids. Proteases and peptidases are often found in laundry detergents -- they help remove things like blood stains from cloth by breaking down the ...
Enteroaggregative Escherichia coli Aggregative - CiteSeerX
... been incriminated as causative agents of persistent childhood diarrhea (9, 38). EAggEC strains are defined by their pattern of mannose-resistant adherence to HEp-2 or HeLa cells. Bacterial aggregates adhere to epithelial cells in a stackedbrick-like lattice, so-called aggregative adherence (AA) (30) ...
... been incriminated as causative agents of persistent childhood diarrhea (9, 38). EAggEC strains are defined by their pattern of mannose-resistant adherence to HEp-2 or HeLa cells. Bacterial aggregates adhere to epithelial cells in a stackedbrick-like lattice, so-called aggregative adherence (AA) (30) ...
Lecture 15: Processing of viral pre-mRNA
... – Can encode 5’ genes, e.g. gag-pol of retroviruses Can be used as ‘genomes’ for packaging inside of nascent viral particles (e.g. retroviruses) ...
... – Can encode 5’ genes, e.g. gag-pol of retroviruses Can be used as ‘genomes’ for packaging inside of nascent viral particles (e.g. retroviruses) ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.