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3.2 Carbohydrates, Lipids and Proteins
3.2 Carbohydrates, Lipids and Proteins

... Waxes – are the third group of lipids. They are formed from long chains of fatty acids joining long-chain alcohol or carbon rings. Waxes are insoluble in water, and are used for water proofing plant leaves or animal feathers and fur. Fats & Diet – stable fats in the body (saturated fats) tend to sta ...
Roots: The origins of molecular genetics: One gene, one enzyme
Roots: The origins of molecular genetics: One gene, one enzyme

... retrospect second only to that of Mendelism itself - it had no impact on genetics. Garrod shares with Mendel the distinction of being the father of a science that became aware of him only after he had passed from the scene and after his work had been repeated independently by others. In Garrod’s cas ...
High-molecular-mass, iron-repressed cytoplasmic proteins in
High-molecular-mass, iron-repressed cytoplasmic proteins in

... siderophore, has already been characterized [9], as have some amino acid-activating enzymatic activities in Azotobacter, which suggest the involvement of a non-ribosomal pathway for the biosynthesis of the pyoverdine-like azotobactin siderophore [IO]. Finally, the recently published nucleotide seque ...
Integrating Functional Genomic Information into the Saccharomyces Genome Database.
Integrating Functional Genomic Information into the Saccharomyces Genome Database.

... are cellular components. Even at the level of a single gene, distinguishing between functions and processes enhances its annotation; for example, we can state that a gene encodes a protein kinase (a function) and is involved in cell cycle progression and cellular morphogenesis (two biological proces ...
Find the gene
Find the gene

... Study the entry How many basepairs (bp) long is the nucleotide sequence displayed? 626bp At what nucleotide position is the start codon located? That is the position where the coding sequence of the mRNA (CDS) begins. 51 Where does the coding sequence end? 494 How many nucletoides long is the coding ...
www.mbio.ncsu.edu
www.mbio.ncsu.edu

... between genes PSHAa1505 and PSHAa1558) is specific to P. haloplanktis. We also identified one specific region in chrI coding for several calcium-dependent proteins, as well as a specific gene in chrII that may regulate cell volume and resistance to cold conditions (PSHAb0555). ...
The role of cytosolic proteins in the insertion of tail
The role of cytosolic proteins in the insertion of tail

... Thus, TA proteins are obliged to use a post-translational mechanism for their insertion into the ER membrane (Borgese et al., 2007; Borgese et al., 2003). In recent years, the introduction of new translocation assays, which distinguish between true transmembrane integration of TA proteins and their ...
Non-human Primate Schlafen11 Inhibits Production of Both
Non-human Primate Schlafen11 Inhibits Production of Both

... translation of viral but not host proteins. This was shown to be related to the different patterns of codon usage observed between host and retroviral transcripts. It has long been known that many RNA viruses do not have the same codon bias as their hosts [16]. Reasons proposed for this differential ...
The Structure and Function of Large Biological Molecules
The Structure and Function of Large Biological Molecules

... polymers have many more monomers than the number of letters in even the longest word. Proteins, for example, are built from 20 kinds of amino acids arranged in chains that are typically hundreds of amino acids long. The molecular logic of life is simple but elegant: Small molecules common to all org ...
7.22 Example Problems for Exam 1 The exam will be of this format. It
7.22 Example Problems for Exam 1 The exam will be of this format. It

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The production of pharmaceutical proteins from the milk of
The production of pharmaceutical proteins from the milk of

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I will henceforth cover the importance of eating simpler meals versus
I will henceforth cover the importance of eating simpler meals versus

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Mesoderm tissue development in Drosophila melanogaster Abstract
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NCEA Level 2 Biology (91159) 2012 Assessment Schedule
NCEA Level 2 Biology (91159) 2012 Assessment Schedule

... Understanding of transcription is shown by the base-pairing rules for DNA and RNA. Sequences are completed to be: TACCGTCTAAGA ATGGCAGATTCT ...
Part 2
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Proteomic Strategies to Analyze Cell
Proteomic Strategies to Analyze Cell

... containing human AB serum were subjected to activation for 24hrs using Staphylococcal enterotoxin B. Cell-free fractions from the activated and control cells were fractionated by twodimensional chromatography in the liquid and intact phase. To improve the sensitivity of detection of protein signatur ...
Protein splicing elements: inteins and exteins
Protein splicing elements: inteins and exteins

... Endonucleases are named by using the first letter of the genus followed by a 2 letter species abbreviation. Intein-derived endonucleases should be named as above and numbered in order of discovery. However, intein homing endonucleases should be numbered independently from RNA-derived homing endonucl ...
Milestone4
Milestone4

... However, the idea of a universal molecular clock has always been a controversial one. For example, it is not easy to reconcile the observation that macroevolution appears to happen suddenly and at irregular intervals (a phenomenon known as punctuated equilibrium) with the idea that mutations, the ra ...
Lecture 4 - University of California, Santa Cruz
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... In practice, specific recognition by each antibody results in precipitation of the red-blood cells. This is because each antibody actually has two functional binding sites enabling extensive crosslinking to occur. ...
Examining the Process of de Novo Gene Birth
Examining the Process of de Novo Gene Birth

... contrast, the products of conserved genes are likely to have developed stable structures. A good starting point at which to address the structure of genes as they age is to examine the predicted secondary structures of the protein products from all different conservation levels. As every protein is ...
Increased MS Protein Identifi cation Rates Using 75 cm Long nano
Increased MS Protein Identifi cation Rates Using 75 cm Long nano

... mammalian cell lysate. More importantly, longer columns showed better reproducibility as seen by increased correlation among technical replicates, higher numbers of quantifiable peptides, and a smaller coefficient of variance (CV), resulting in improved protein quantification for complex lysates by ...
Document
Document

... assays can be used to gain information about how a particular section of DNA drives gene expression in isolation from a chromosomal context. There are advantages and disadvantages associated with using transient analysis. The obvious disadvantage is that promoters do not always behave in the same fa ...
Full Text  - Molecular Biology and Evolution
Full Text - Molecular Biology and Evolution

... clades sharing it increases. We find that the more universal the protein, the less likely it is to be membrane-bound (fig. 4). Since ortholog discovery depends on the successful detection of homologs using tools such as BLAST, the lower homology of membrane proteins we report could have two main cau ...
Not just another hole in the wall: understanding intercellular protein
Not just another hole in the wall: understanding intercellular protein

... the endodermal cell layer and normal patterning of the root (Helariutta et al. 2000). Recently, SHR movement was shown to be dependent upon its presence in the cytoplasm (Gallagher et al. 2004). SHR is expressed in stele cells in the Arabidopsis root meristem. In these cells the SHR protein localize ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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