Molecular targets of oxidative stress
... potential role of oxidative protein damage in ROS-mediated cell killing has been less well covered. The available data establish that certain proteins are more susceptible to oxidative targeting than others. The factors determining such selectivity include the relative content of oxidation-sensitive ...
... potential role of oxidative protein damage in ROS-mediated cell killing has been less well covered. The available data establish that certain proteins are more susceptible to oxidative targeting than others. The factors determining such selectivity include the relative content of oxidation-sensitive ...
Why genes are regulated?
... Why genes are regulated? • Minimize energy consumption--why express a gene you do not need? (economy) • Control growth--many cells in a mature organism do not grow, and expression of genes involved in promoting cell division is tightly regulated. (physiological balance) • Development--inappropriate ...
... Why genes are regulated? • Minimize energy consumption--why express a gene you do not need? (economy) • Control growth--many cells in a mature organism do not grow, and expression of genes involved in promoting cell division is tightly regulated. (physiological balance) • Development--inappropriate ...
Leukaemia Section t(5;12)(q33;p13) ERC1/PDGFRB Atlas of Genetics and Cytogenetics
... Membrane receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Signal transduction. ...
... Membrane receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Signal transduction. ...
Vilar et al. 2006, PLoS Computational Biology
... which possess any single or combination of characteristics favourable to drug development, such as: (1) conserved sequence, (2) tissue-specificity, and (3) expression domain not overlapping with other members of the family. Conserved sequence suggests that function is the same, and that drugs will h ...
... which possess any single or combination of characteristics favourable to drug development, such as: (1) conserved sequence, (2) tissue-specificity, and (3) expression domain not overlapping with other members of the family. Conserved sequence suggests that function is the same, and that drugs will h ...
PathogenBioinformatics
... This table lists proteins upstream and downstream of the target protein LLO. Each protein is labeled with its function and location on the strain (beginning and ending base pair numbers). In the right-most column labeled “Aliases” are links to other bioinformatics sites. Notice the column heading "f ...
... This table lists proteins upstream and downstream of the target protein LLO. Each protein is labeled with its function and location on the strain (beginning and ending base pair numbers). In the right-most column labeled “Aliases” are links to other bioinformatics sites. Notice the column heading "f ...
Caloric value
... 4. Contractile – movement (muscles) 5. Enzymatic – speed up chem. reactions (saliva) ...
... 4. Contractile – movement (muscles) 5. Enzymatic – speed up chem. reactions (saliva) ...
Membrane Proteins: Capturing the signal | eLife
... bout a quarter of eukaryotic genes encode membrane proteins, many of which perform activities that are essential for cells to survive (Wallin and von Heijne, 1998). Nevertheless, the production of these proteins poses a problem for the cell. Like all proteins, membrane proteins are built by ribosome ...
... bout a quarter of eukaryotic genes encode membrane proteins, many of which perform activities that are essential for cells to survive (Wallin and von Heijne, 1998). Nevertheless, the production of these proteins poses a problem for the cell. Like all proteins, membrane proteins are built by ribosome ...
Chapter 3C Notes from Book
... Figure 3.24 Microtubules and microfilaments function in cell motility by interacting with motor molecules powered by ATP. ...
... Figure 3.24 Microtubules and microfilaments function in cell motility by interacting with motor molecules powered by ATP. ...
cDNA-derived molecular characteristics and antibodies to a new
... genes that belong to group (a) are detectable by the differential screening approach. The majority of the known cell cycle phase-dependent and transcriptionally regulated genes are those required for DNA synthesis, coordinating DNA replication tightly with the cell cycle. The G2/M transition is anot ...
... genes that belong to group (a) are detectable by the differential screening approach. The majority of the known cell cycle phase-dependent and transcriptionally regulated genes are those required for DNA synthesis, coordinating DNA replication tightly with the cell cycle. The G2/M transition is anot ...
Adaptive Silver Films for Detection of Antibody Binding
... features unique to different conformational states, which is in agreement with X-ray crystallographic studies.20 In this paper we demonstrate that SERS substrates based on nanostructured adaptive silver films (ASFs) make it possible to detect the formation of specific antigen-antibody complexes at a ...
... features unique to different conformational states, which is in agreement with X-ray crystallographic studies.20 In this paper we demonstrate that SERS substrates based on nanostructured adaptive silver films (ASFs) make it possible to detect the formation of specific antigen-antibody complexes at a ...
SISYPHUS—structural alignments for proteins with non
... on a detailed analysis of protein structures and their definitions were manually derived. The design of each alignment in the SISYPHUS database includes several steps. Initially, we performed multiple pairwise superimpositions of all protein regions used to construct a particular multiple alignment. ...
... on a detailed analysis of protein structures and their definitions were manually derived. The design of each alignment in the SISYPHUS database includes several steps. Initially, we performed multiple pairwise superimpositions of all protein regions used to construct a particular multiple alignment. ...
Mapping of Lipid-‐Binding Proteins and Their Ligandability in Cells
... Arachidonic acid derived molecules mediate both Nature physiological and Cellacid (AA, C20:4), from phospholipids (X stands for a phospholipid headgroup; see figure) or diacylglycerol (not shown; see ...
... Arachidonic acid derived molecules mediate both Nature physiological and Cellacid (AA, C20:4), from phospholipids (X stands for a phospholipid headgroup; see figure) or diacylglycerol (not shown; see ...
Introduction to Endocrinology
... glycoproteins; penetrate through membrane For protein & charged hormones (peptides or neurotransmitters) What are the main types of receptors? ...
... glycoproteins; penetrate through membrane For protein & charged hormones (peptides or neurotransmitters) What are the main types of receptors? ...
The proteome of the infectious bronchitis virus Beau
... precipitation and sucrose-gradient ultracentrifugation. First, the virions were concentrated, by precipitation from allantoic fluid, by the addition of PEG-8000 to a final concentration of 10 %. The precipitate was reconstituted and loaded onto a sucrose gradient for purification. IBV virions formed ...
... precipitation and sucrose-gradient ultracentrifugation. First, the virions were concentrated, by precipitation from allantoic fluid, by the addition of PEG-8000 to a final concentration of 10 %. The precipitate was reconstituted and loaded onto a sucrose gradient for purification. IBV virions formed ...
an introduction to endocrinology - Home
... glycoproteins; penetrate through membrane For protein & charged hormones (peptides or neurotransmitters) What are the main types of receptors? ...
... glycoproteins; penetrate through membrane For protein & charged hormones (peptides or neurotransmitters) What are the main types of receptors? ...
subunits of Snf1 kinase are required for kinase function and
... the b-subunits in both yeast and mammals is myristoylated, it is reasonable to think that the b-subunits may also play a role in controlling the subcellular localization of alternate forms of the kinase in vivo. Yeast encode three b-subunit genes, SIP1, SIP2 and GAL83. Immunoprecipitation studies ha ...
... the b-subunits in both yeast and mammals is myristoylated, it is reasonable to think that the b-subunits may also play a role in controlling the subcellular localization of alternate forms of the kinase in vivo. Yeast encode three b-subunit genes, SIP1, SIP2 and GAL83. Immunoprecipitation studies ha ...
The extracellular matix (ECM) Three types of molecules are
... – It forms enormous complexes with proteoglycans in the extracellular matrix. These complexes are especially abundant in cartilage. – There, hyaluronan is associated with the proteoglycan aggrecan, via a linker protein. Hyaluronan is highly negatively charged. – It binds to cations and water in the ...
... – It forms enormous complexes with proteoglycans in the extracellular matrix. These complexes are especially abundant in cartilage. – There, hyaluronan is associated with the proteoglycan aggrecan, via a linker protein. Hyaluronan is highly negatively charged. – It binds to cations and water in the ...
2-D Quant Kit - GE Healthcare Life Sciences
... Electrophoretic analysis of protein samples requires accurate quantification of the sample to be analyzed. This assures that an appropriate amount of protein is loaded. In addition, accurate quantification facilitates comparison among similar samples by allowing identical amounts of protein to be lo ...
... Electrophoretic analysis of protein samples requires accurate quantification of the sample to be analyzed. This assures that an appropriate amount of protein is loaded. In addition, accurate quantification facilitates comparison among similar samples by allowing identical amounts of protein to be lo ...
Pro
... b) The preprocollagen polypeptide is released into the cytoplasm. c) A special sequence on the N-terminus called the ‘signal peptide’ directs the preprocollagen to a pore in the ER through which it enters the ER. ...
... b) The preprocollagen polypeptide is released into the cytoplasm. c) A special sequence on the N-terminus called the ‘signal peptide’ directs the preprocollagen to a pore in the ER through which it enters the ER. ...
list of publications
... their mode of action, it remains unknown how any of these proteins recognizes and functions. The basis of this diversity is differential gene expression, and and distinguishes chromatin domains containing genes to be activated or different cell types are characterized by distinct gene expression pat ...
... their mode of action, it remains unknown how any of these proteins recognizes and functions. The basis of this diversity is differential gene expression, and and distinguishes chromatin domains containing genes to be activated or different cell types are characterized by distinct gene expression pat ...
Presence and Absence of COX8 in Reptile Transcriptomes
... was important for optimal mitochondrial performance. Interestingly, several lizard species have apparently lost the gene encoding for this protein. The goal of this research was to assess the phylogenetic extent of the loss of COX8. To do so we looked for traces of the COX8 coding gene using bioinfo ...
... was important for optimal mitochondrial performance. Interestingly, several lizard species have apparently lost the gene encoding for this protein. The goal of this research was to assess the phylogenetic extent of the loss of COX8. To do so we looked for traces of the COX8 coding gene using bioinfo ...
Intrinsic Disorder in Cell-signaling and Cancer
... qualitatively, we observed similar disorder prediction results for both (compare Figures 1(a) and 2(b)). Our analysis applied to these two independently constructed sets strongly supports the increased amount of disorder in proteins involved in cell-signaling and regulation. Interestingly, the prote ...
... qualitatively, we observed similar disorder prediction results for both (compare Figures 1(a) and 2(b)). Our analysis applied to these two independently constructed sets strongly supports the increased amount of disorder in proteins involved in cell-signaling and regulation. Interestingly, the prote ...
- Wiley Online Library
... identified. Because yeast cells with deleted SMT3 gene are complemented by the human SUMO1 gene, it is assumed that the SMT3 gene is a yeast orthologue of the human SUMO1 (Johnson, 2004). In yeast SUMO conjugation process, Siz1 and Siz2 SUMO ligases are required for the majority of substrates (Johns ...
... identified. Because yeast cells with deleted SMT3 gene are complemented by the human SUMO1 gene, it is assumed that the SMT3 gene is a yeast orthologue of the human SUMO1 (Johnson, 2004). In yeast SUMO conjugation process, Siz1 and Siz2 SUMO ligases are required for the majority of substrates (Johns ...
Ch6-4_Enzymes-New
... A competitive inhibitor molecule occupies the active site and blocks entry of the substrate. ...
... A competitive inhibitor molecule occupies the active site and blocks entry of the substrate. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.