High Efficiency Transient Expression System for
... interest and another carrying a standard binary expression vector for a Suppressor of gene silencing, extremely high level expression of the gene of interest can be achieved. Expression also persists for much longer, up to 12 days post inoculation and longer. Indeed the time period of expression may ...
... interest and another carrying a standard binary expression vector for a Suppressor of gene silencing, extremely high level expression of the gene of interest can be achieved. Expression also persists for much longer, up to 12 days post inoculation and longer. Indeed the time period of expression may ...
Revised Manuscript - Open Research Exeter
... stage 10 minutes following silver shock at a sub-inhibitory dose to reduce background gene ...
... stage 10 minutes following silver shock at a sub-inhibitory dose to reduce background gene ...
The Genetics of Beta-galactosidase-
... substrates or metabolites. Before divulging the body of evidence that was used to derive the operon model, Jacob and Monod stated their conclusions up front. They concluded that: in general, enzyme systems were subject to negative control; two new types of genetic determinants, the regulator and ope ...
... substrates or metabolites. Before divulging the body of evidence that was used to derive the operon model, Jacob and Monod stated their conclusions up front. They concluded that: in general, enzyme systems were subject to negative control; two new types of genetic determinants, the regulator and ope ...
Leukaemia Section t(8;13)(p12;q12) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Aguiar RC, Chase A, Coulthard S, Macdonald DH, Carapeti M, Reiter A, Sohal J, Lennard A, Goldman JM, Cross NC. Abnormalities of chromosome band 8p11 in leukemia: two clinical syndromes can be distinguished on the basis of MOZ involvement. Blood 1997;90:3130-35. ...
... Aguiar RC, Chase A, Coulthard S, Macdonald DH, Carapeti M, Reiter A, Sohal J, Lennard A, Goldman JM, Cross NC. Abnormalities of chromosome band 8p11 in leukemia: two clinical syndromes can be distinguished on the basis of MOZ involvement. Blood 1997;90:3130-35. ...
Risk assessment of Genetically Modified Micro-Organisms
... It is not appropriate to consider non-disabled pathogens of plants, humans, animals or insects as inherently safe recipient micro-organisms. Examples of inherently safe recipient microorganisms which, depending on the nature of the insert, would in most cases be expected to form the basis of extreme ...
... It is not appropriate to consider non-disabled pathogens of plants, humans, animals or insects as inherently safe recipient micro-organisms. Examples of inherently safe recipient microorganisms which, depending on the nature of the insert, would in most cases be expected to form the basis of extreme ...
PKU: GENETICS AND INHERITANCE
... The chance for a child to have PKU depends on the partner 2% (1 in 50) of the population is a carrier for PKU If unknown whether the partner is a carrier, the chance to have a child with PKU is 1 in 100 (1%) Carrier testing is available for partners ...
... The chance for a child to have PKU depends on the partner 2% (1 in 50) of the population is a carrier for PKU If unknown whether the partner is a carrier, the chance to have a child with PKU is 1 in 100 (1%) Carrier testing is available for partners ...
Plant mitochondria contain the protein translocase subunits TatB
... membrane, indicating that AtmtTatB is imported into the matrix or inner membrane of plant mitochondria. When the outer membrane was removed prior to the addition of proteinase K, AtmtTatB remained protease-resistant, indicating that if it is located in the inner mitochondrial membrane, the majority ...
... membrane, indicating that AtmtTatB is imported into the matrix or inner membrane of plant mitochondria. When the outer membrane was removed prior to the addition of proteinase K, AtmtTatB remained protease-resistant, indicating that if it is located in the inner mitochondrial membrane, the majority ...
The protein import apparatus of chloroplasts
... protein from pea and spinach occurs into a proteascinsensitive localization in the envelope memhranes (Fliigge et a!. 1989, Willey et a!. 1991). (3) Thc phosphate translocator activity and the protein import efficiency is largely diminished by incubating the chloroplas!s with 4,4' -diisothiocyanosti ...
... protein from pea and spinach occurs into a proteascinsensitive localization in the envelope memhranes (Fliigge et a!. 1989, Willey et a!. 1991). (3) Thc phosphate translocator activity and the protein import efficiency is largely diminished by incubating the chloroplas!s with 4,4' -diisothiocyanosti ...
AHM1, a Novel Type of Nuclear Matrix–Localized
... The nuclear matrix, operationally defined, is the dynamic fibrogranular structure forming the skeletal framework that surrounds and penetrates the interphase nucleus; it has been implicated in most nuclear functions, including replication, repair, transcription, RNA processing, and RNA transport (Be ...
... The nuclear matrix, operationally defined, is the dynamic fibrogranular structure forming the skeletal framework that surrounds and penetrates the interphase nucleus; it has been implicated in most nuclear functions, including replication, repair, transcription, RNA processing, and RNA transport (Be ...
The amdR product and a CCAAT-binding factor
... is clear that plam28 reduces the intensity of band (b) more efficiently than pUC18 alone (Fig. 4, lane 5) and that pIR12 does so even more efficiently (Fig. 4, lane 4). The specific competition by these sequences is consistent with the protein involved in band (b) being the amdR gene product. The am ...
... is clear that plam28 reduces the intensity of band (b) more efficiently than pUC18 alone (Fig. 4, lane 5) and that pIR12 does so even more efficiently (Fig. 4, lane 4). The specific competition by these sequences is consistent with the protein involved in band (b) being the amdR gene product. The am ...
SecStAnT: secondary structure analysis tool for data selection
... and space scales, needing large parallelism. This problem is not likely to be simply resolved by the increase of the processors power and of parallelism, becoming increasingly harder as the system complexity grows. Recent efforts have focused on the development of dedicated hardware. An example is t ...
... and space scales, needing large parallelism. This problem is not likely to be simply resolved by the increase of the processors power and of parallelism, becoming increasingly harder as the system complexity grows. Recent efforts have focused on the development of dedicated hardware. An example is t ...
Molecular Weight Estimation - Bio-Rad
... are visible following development of the chemiluminescent signal. Additionally, the ability to visualize protein standards (for example, by using prestained standards) during the electrophoresis run and directly on the blot following the transfer step is also advantageous. Prestained protein standar ...
... are visible following development of the chemiluminescent signal. Additionally, the ability to visualize protein standards (for example, by using prestained standards) during the electrophoresis run and directly on the blot following the transfer step is also advantageous. Prestained protein standar ...
the molecules of life - Betavak-NLT
... pancreas. The cause of these problems seems to lie with the mucus-producing cells. These cells, which can be found in the lungs, intestines and pancreas, do not function properly. They produce inadequate amounts of mucus as well as sticky, thick mucus. To understand precisely where the problem lies, ...
... pancreas. The cause of these problems seems to lie with the mucus-producing cells. These cells, which can be found in the lungs, intestines and pancreas, do not function properly. They produce inadequate amounts of mucus as well as sticky, thick mucus. To understand precisely where the problem lies, ...
Linking Cataracts to Cancer
... associated with the different types of cancer, then they will learn to predict premature cancer through simply observing the proteins and genes in an individual’s body. The protein that is the primary focus of this project is referred to as alpha-basic crystallin (αb- crystallin). Αb- crystallin is ...
... associated with the different types of cancer, then they will learn to predict premature cancer through simply observing the proteins and genes in an individual’s body. The protein that is the primary focus of this project is referred to as alpha-basic crystallin (αb- crystallin). Αb- crystallin is ...
1 a dictyostelium mutant with reduced lysozyme levels compensates
... isoform to compensate for their deficiency, they remain much more sensitive to infections of the lung (14). Lysozymes are not only found in animals but are also present in numerous phylogenetically diverse organisms such as plants, fungi, bacteria, and bacteriophages (15). Several different classes ...
... isoform to compensate for their deficiency, they remain much more sensitive to infections of the lung (14). Lysozymes are not only found in animals but are also present in numerous phylogenetically diverse organisms such as plants, fungi, bacteria, and bacteriophages (15). Several different classes ...
TEL1, a Gene Involved in Controlling Telomere Length in S
... types of chromosome aberrations found in AT cells is endSPresent address: Department of Psychiatry, Universityof illinois at Chicago, Chicago, Illinois 60612. ...
... types of chromosome aberrations found in AT cells is endSPresent address: Department of Psychiatry, Universityof illinois at Chicago, Chicago, Illinois 60612. ...
region of the Bacillus subtilis chromosome containing genes
... previously from random cloning of pYAC10-8 DNA, was used to probe the A phage library for clones within the region between tbrS and trx, resulting in the isolation of A phage SC9. The sequence obtained from ASC9 was used to obtain LR PCR products 1inkingyseC to tbrS on one side andpbeT to trx on the ...
... previously from random cloning of pYAC10-8 DNA, was used to probe the A phage library for clones within the region between tbrS and trx, resulting in the isolation of A phage SC9. The sequence obtained from ASC9 was used to obtain LR PCR products 1inkingyseC to tbrS on one side andpbeT to trx on the ...
Novel genes involved in the regulation of
... which includes rpfD and rpfE. This analysis has identified a further gene (orf4) involved in the regulation of extracellular enzyme and EPS synthesis, together with three ORFs (orf1, 2 and 3) of unknown function. In addition, two genes were identified whose products are highly related to RecJ and Gr ...
... which includes rpfD and rpfE. This analysis has identified a further gene (orf4) involved in the regulation of extracellular enzyme and EPS synthesis, together with three ORFs (orf1, 2 and 3) of unknown function. In addition, two genes were identified whose products are highly related to RecJ and Gr ...
Bioinformatics 3 V 5 – Robustness and Modularity
... Analysis of meso-scale properties demonstrated the presence of highly connected clusters of proteins in a network of protein interactions -> strongly supports suggested modular architecture of biological networks. There exist 2 types of clusters: protein complexes and dynamic functional modules. Bot ...
... Analysis of meso-scale properties demonstrated the presence of highly connected clusters of proteins in a network of protein interactions -> strongly supports suggested modular architecture of biological networks. There exist 2 types of clusters: protein complexes and dynamic functional modules. Bot ...
MND Australia International Research Update December 2016
... to discover much about the causes of disease by studying the genetic mutations these people carry. However, in the remaining 90% of cases, there is no genetic history of disease, and thus the causes of this sporadic form of MND have remained elusive. Nevertheless, what we do know of both sporadic MN ...
... to discover much about the causes of disease by studying the genetic mutations these people carry. However, in the remaining 90% of cases, there is no genetic history of disease, and thus the causes of this sporadic form of MND have remained elusive. Nevertheless, what we do know of both sporadic MN ...
Gene Section SSX2 (Synovial Sarcoma, X breakpoint 2) in Oncology and Haematology
... chromatin compaction, DNA methylation, repressive histone modifications and inaccessibility of promoter regions to transcription machineries. Other SSX2interacting partners include the LIM homeobox protein LHX4, a Ras-like GTPase Interactor, RAB3IP thought to be involved in vesicular transport, and ...
... chromatin compaction, DNA methylation, repressive histone modifications and inaccessibility of promoter regions to transcription machineries. Other SSX2interacting partners include the LIM homeobox protein LHX4, a Ras-like GTPase Interactor, RAB3IP thought to be involved in vesicular transport, and ...
The Pex16p Homolog SSE1 and Storage Organelle
... 367 amino acids (Fig. 3A). Expression of SSE1 cDNA in transgenic sse1 plants (10) complements the shrunken seed phenotype (Fig. 3B). Similar to wild type, seeds are tolerant of desiccation and cells are filled with storage proteins and lipids, but lack starch. The SSE1 sequence showed similarity to ...
... 367 amino acids (Fig. 3A). Expression of SSE1 cDNA in transgenic sse1 plants (10) complements the shrunken seed phenotype (Fig. 3B). Similar to wild type, seeds are tolerant of desiccation and cells are filled with storage proteins and lipids, but lack starch. The SSE1 sequence showed similarity to ...
Document
... over and chromosome fragmentation, respectively. Since the proteins that perform these processes share similar functions, it is possible the same mechanism of regulation is used. Therefore, cyclin gene T.Therm_00189230 could control and recruit the proteins necessary for this mechanism of genome rea ...
... over and chromosome fragmentation, respectively. Since the proteins that perform these processes share similar functions, it is possible the same mechanism of regulation is used. Therefore, cyclin gene T.Therm_00189230 could control and recruit the proteins necessary for this mechanism of genome rea ...
Document
... Organic Molecules = Living Molecules Defined by the Presence of Carbon that acts to Hold Together these large, complex molecules. ...
... Organic Molecules = Living Molecules Defined by the Presence of Carbon that acts to Hold Together these large, complex molecules. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.