Fulltext PDF - Indian Academy of Sciences
... TaPIEP1, a pathogen-induced ERF gene, confers resistance to Bipolaris sorokiniana in wheat (Dong et al. 2010). The expression of NtERF5 increases tolerance to TMV (Fischer and Dröge-Laser, 2004). The overexpression of OPBP1 exhibits distinct enhancing of resistance to Magnaporthe oryzae and Rhizocto ...
... TaPIEP1, a pathogen-induced ERF gene, confers resistance to Bipolaris sorokiniana in wheat (Dong et al. 2010). The expression of NtERF5 increases tolerance to TMV (Fischer and Dröge-Laser, 2004). The overexpression of OPBP1 exhibits distinct enhancing of resistance to Magnaporthe oryzae and Rhizocto ...
2006 7.012 Problem Set 3 KEY
... Bacteria only need to synthesize tryptophan when there is none available in the environment. If there is some available already, it makes a lot more sense in terms of energy consumption to just take it up from the environment, instead of synthesize it from scratch. (b) A mutant bacterium has no acti ...
... Bacteria only need to synthesize tryptophan when there is none available in the environment. If there is some available already, it makes a lot more sense in terms of energy consumption to just take it up from the environment, instead of synthesize it from scratch. (b) A mutant bacterium has no acti ...
CHAPTER 17 Regulation of Gene Expression in Eukaryotes
... ii. Samples were treated with different concentrations of DNase I. iii. DNA was then extracted, digested with BamHI and Southern blotted with globin DNA as the probe. iv. The result was gradual disappearance of the globin DNA during DNase I treatment (indicating that the DNA was loosened enough to a ...
... ii. Samples were treated with different concentrations of DNase I. iii. DNA was then extracted, digested with BamHI and Southern blotted with globin DNA as the probe. iv. The result was gradual disappearance of the globin DNA during DNase I treatment (indicating that the DNA was loosened enough to a ...
The Amino Terminus of the Yeast F1-ATPase {j
... University). The unique Sma 1/Xma I site normally present in the URA3 DNA segment (3' of the structural gene) contained in this plasmid was removed by digestion with Xma I followed by Sl nuclease treatment and ligation with T4 DNA ligase. This Sma 1/Xma I-deleted derivative of YCp50 was then digeste ...
... University). The unique Sma 1/Xma I site normally present in the URA3 DNA segment (3' of the structural gene) contained in this plasmid was removed by digestion with Xma I followed by Sl nuclease treatment and ligation with T4 DNA ligase. This Sma 1/Xma I-deleted derivative of YCp50 was then digeste ...
Document
... • N-formylmethionine in prokaryotes is _______________ • specific bonds in precursors are cleaved, as for example, preproinsulin to proinsulin to insulin • ___________ _________are removed by specific proteases of the endoplasmic reticulum; the Golgi apparatus then directs the finished protein to it ...
... • N-formylmethionine in prokaryotes is _______________ • specific bonds in precursors are cleaved, as for example, preproinsulin to proinsulin to insulin • ___________ _________are removed by specific proteases of the endoplasmic reticulum; the Golgi apparatus then directs the finished protein to it ...
Macromolecules & Enzymes
... The changing of one set of chemicals into another set of chemicals Reactants start the reaction. Products are produced by the reactants. Ex: CO2 + H2O H2CO3 Reactants (before arrow) ...
... The changing of one set of chemicals into another set of chemicals Reactants start the reaction. Products are produced by the reactants. Ex: CO2 + H2O H2CO3 Reactants (before arrow) ...
Powerpoint slides - School of Engineering and Applied Science
... Much of the protein core comprises helices and sheets, folded into a threedimensional configuration: ...
... Much of the protein core comprises helices and sheets, folded into a threedimensional configuration: ...
Electrophoresis
... It can be used analytically to study the properties of a single charged species or mixtures of molecules. It can also be used preparatively as a separating technique ...
... It can be used analytically to study the properties of a single charged species or mixtures of molecules. It can also be used preparatively as a separating technique ...
P8100Datasheet-Lot0041208
... Molecular Weight: 29849 daltons Reconstitution: Endoproteinase GluC should be reconstituted by the addition of 50–500 µl of high purity water. Rapid autolysis is a function of enzyme concentration. ...
... Molecular Weight: 29849 daltons Reconstitution: Endoproteinase GluC should be reconstituted by the addition of 50–500 µl of high purity water. Rapid autolysis is a function of enzyme concentration. ...
Elastin
... connective tissues. It is inherited as an autosomal dominant trait. It affects the eyes, the skeletal system and hyper-extensibility of the joints and cardiovascular system. This syndrome is due to mutations in the gene for fibrilin. With this disease, abnormal fibrillin protein is incorporated into ...
... connective tissues. It is inherited as an autosomal dominant trait. It affects the eyes, the skeletal system and hyper-extensibility of the joints and cardiovascular system. This syndrome is due to mutations in the gene for fibrilin. With this disease, abnormal fibrillin protein is incorporated into ...
Gene Section
... domain. The presence of these kinds of sequences seems to be necessary for maturation toward the TCRgd lineage, whereas their absence leads to maturation arrest at a more immature stage. Abnormal Protein Both CALM-AF10 and the reciprocal AF10-CALM are expressed. However, the CALM-AF10 contains most ...
... domain. The presence of these kinds of sequences seems to be necessary for maturation toward the TCRgd lineage, whereas their absence leads to maturation arrest at a more immature stage. Abnormal Protein Both CALM-AF10 and the reciprocal AF10-CALM are expressed. However, the CALM-AF10 contains most ...
A statistical physics perspective on alignment-independent pro
... decades, and thus an enormous associated literature has accumulated. Typically, substitution matrices specify a score for aligning pairs of nucleotides or amino acids; in such matrices different amino acids or nucleotides score differently according to the potential likelihood that one will replace ...
... decades, and thus an enormous associated literature has accumulated. Typically, substitution matrices specify a score for aligning pairs of nucleotides or amino acids; in such matrices different amino acids or nucleotides score differently according to the potential likelihood that one will replace ...
CH 17_ From Gene to Protein
... • Two populations of ribosomes are evident in cells: free ribsomes (in the cytosol) and bound ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cel ...
... • Two populations of ribosomes are evident in cells: free ribsomes (in the cytosol) and bound ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cel ...
Mislocalization and inhibition of acetyl
... to malonyl-CoA, a rate-limiting step in fatty acid biosynthesis, through two enzymatic steps, biotin carboxylation and carboxyl group transfer. Two isoforms of ACC have been identified in mammals: ACC1 (ACCα, 265 kDa) and ACC2 (ACCβ, 280 kDa) [3–6]. The two isoforms have the same enzymatic function ...
... to malonyl-CoA, a rate-limiting step in fatty acid biosynthesis, through two enzymatic steps, biotin carboxylation and carboxyl group transfer. Two isoforms of ACC have been identified in mammals: ACC1 (ACCα, 265 kDa) and ACC2 (ACCβ, 280 kDa) [3–6]. The two isoforms have the same enzymatic function ...
A simple calculus for proteins and cells
... proposed in [2]. According to these principles, bonds between proteins are represented in π-calculus by shared channel names and protein interactions are modelled by process communications. The κ-calculus [3] belongs to this family and it has been showed convenient for representing mechanisms such a ...
... proposed in [2]. According to these principles, bonds between proteins are represented in π-calculus by shared channel names and protein interactions are modelled by process communications. The κ-calculus [3] belongs to this family and it has been showed convenient for representing mechanisms such a ...
Regulation
... – RNA-poly binds freely to promoter – Constitutive genes— Enzymes always needed (e.g., glycolysis) • Negative gene regulation – Repressor protein binds operator → Block RNA polymerase → Inhibits gene expression → Decreases synthesis of enzymes • Positive gene regulation – Activator protein binds sep ...
... – RNA-poly binds freely to promoter – Constitutive genes— Enzymes always needed (e.g., glycolysis) • Negative gene regulation – Repressor protein binds operator → Block RNA polymerase → Inhibits gene expression → Decreases synthesis of enzymes • Positive gene regulation – Activator protein binds sep ...
Enhanced Bioaccumulation of Heavy Metals by Bacterial Cells Displaying Synthetic Phytochelatins
... MTs in bacterial cells results in enhanced metal accumulation and thus offers a promising strategy for the development of microbial-based biosorbents to remediate metal contamination (Kille et al., 1991; Pazirandeh et al., 1995; Romeyer et al., 1990). However, metal removal by intracellular MTs has ...
... MTs in bacterial cells results in enhanced metal accumulation and thus offers a promising strategy for the development of microbial-based biosorbents to remediate metal contamination (Kille et al., 1991; Pazirandeh et al., 1995; Romeyer et al., 1990). However, metal removal by intracellular MTs has ...
The evolution, function, structure, and expression
... Waters et al., 2008a; Sarkar et al., 2009; Bondino et al., 2012). These include six subfamilies that are cytoplasmic/nuclear localized (CI–CVI) and five sHSP subfamilies that localize to organelles. The organelle subfamilies include one subfamily that localizes to the endoplasmic reticulum (ER), ano ...
... Waters et al., 2008a; Sarkar et al., 2009; Bondino et al., 2012). These include six subfamilies that are cytoplasmic/nuclear localized (CI–CVI) and five sHSP subfamilies that localize to organelles. The organelle subfamilies include one subfamily that localizes to the endoplasmic reticulum (ER), ano ...
The Amino Terminus of the Yeast F1-ATPase B
... Results obtained with the gene fusions and with targeting-defective A TP2 deletion mutants provide evidence that the amino-terminal 27 amino acids of the 13Lsubunit protein precursor are sufficient to direct both specific sorting o f this protein to yeast mitochondria and its import into the organel ...
... Results obtained with the gene fusions and with targeting-defective A TP2 deletion mutants provide evidence that the amino-terminal 27 amino acids of the 13Lsubunit protein precursor are sufficient to direct both specific sorting o f this protein to yeast mitochondria and its import into the organel ...
EXB0014 Bovine Serum Albumin – Dyomics 547 Lyophilized
... Bovine Serum Albumin (BSA) is a 66 kDa large globular protein with a good essential amino acid profile. Isolated BSA has been reported to be a very functional protein (Waniska et al., 1981). Its primary biological function has been associated with its lipid binding properties (Fox and Flynn, 1992), ...
... Bovine Serum Albumin (BSA) is a 66 kDa large globular protein with a good essential amino acid profile. Isolated BSA has been reported to be a very functional protein (Waniska et al., 1981). Its primary biological function has been associated with its lipid binding properties (Fox and Flynn, 1992), ...
GM Form
... (vi) In the case of organisms whose multiplication involves a complex life-cycle, will the work involve the propagation of organisms that are in stages of that life-cycle that are particularly hazardous? Examples include the propagation of the infective stages of parasites or the release of spores f ...
... (vi) In the case of organisms whose multiplication involves a complex life-cycle, will the work involve the propagation of organisms that are in stages of that life-cycle that are particularly hazardous? Examples include the propagation of the infective stages of parasites or the release of spores f ...
Thesis - u
... 1. Identification of the macromolecules present in Arabidopsis phloem sap We previously showed that PP2-A1 interacts with phloem sap proteins and we carried out a first identification of the phloem proteins present in the sap. We will use the same method to exudate phloem sap and to analyze the popu ...
... 1. Identification of the macromolecules present in Arabidopsis phloem sap We previously showed that PP2-A1 interacts with phloem sap proteins and we carried out a first identification of the phloem proteins present in the sap. We will use the same method to exudate phloem sap and to analyze the popu ...
Chapter 27 Protein Metabolism
... for some amino acids were revealed using mixed copolymers of RNA • The composition of an RNA synthesized using polyribonucleotide phosphorylase depends on the proportion of each NDP present in the reaction mixture. • Investigation of the identity and quantity of the amino acids incorporated into the ...
... for some amino acids were revealed using mixed copolymers of RNA • The composition of an RNA synthesized using polyribonucleotide phosphorylase depends on the proportion of each NDP present in the reaction mixture. • Investigation of the identity and quantity of the amino acids incorporated into the ...
1 ENZYME LABS Introduction: Without enzymes chemical reactions
... and protein molecules in a watery solution. As we discussed in class, proteins are large organic molecules that are built as a chain (or polymer) of amino acids. The behavior and function of the protein is caused by the specific amino acids that are linked together in the chain. These amino acids re ...
... and protein molecules in a watery solution. As we discussed in class, proteins are large organic molecules that are built as a chain (or polymer) of amino acids. The behavior and function of the protein is caused by the specific amino acids that are linked together in the chain. These amino acids re ...
Transport of protein kinase C α into the nucleus requires intact
... Treatment with 0.2 µM vinblastine had very similar effects (data not shown). In resting 3T3 cells PKC α is localized almost completely in the cytoplasm as revealed by immunocytochemistry with the isoform specific monoclonal antibody against PKC α (Fig. 2A). As already observed earlier (Leach et al., ...
... Treatment with 0.2 µM vinblastine had very similar effects (data not shown). In resting 3T3 cells PKC α is localized almost completely in the cytoplasm as revealed by immunocytochemistry with the isoform specific monoclonal antibody against PKC α (Fig. 2A). As already observed earlier (Leach et al., ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.