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Bioreactors and transgenic animals
Bioreactors and transgenic animals

... gives us the hypothetical ability to do things never contemplated before". Team of 20 top scientists, led by the H. Smith has constructed a synthetic chromosome based on the bacterium Mycoplasma genitalium, that is 381 genes long and contains 580,000 base pairs of genetic code. The synthetically rec ...
Antp
Antp

... domains creates thoracic, ribbearing vertebrae along almost entire body length ...
Gene Regulation Topic Guide
Gene Regulation Topic Guide

... digest lactose. If it is on, lactose can be digested. First, draw the lac operon. 14. How many genes does the lac operon have? 15. What is the function of the operon? 16. What is the function of the repressor? 17. Why is this called positive control? 18. What happens to the lac operon when lactose ...
New Insights into Polycistronic Transcripts in Eukaryotes
New Insights into Polycistronic Transcripts in Eukaryotes

... regions supports the hypothesis that they are transcribed as a polycistronic transcript. Since dimeric or multimeric proteins are required for gustatory receptors of sugars, co-expression of six genes could result in various combinational receptors to discriminate between the different sugars. Moreo ...
Crossing Membranes – Passive Processes
Crossing Membranes – Passive Processes

... bilayer. E.g. O2, CO2 and steroid hormones • Other very small charged particles like water and small ions can also diffuse directly through the lipid bilayer. ...
Functional Anthology of Intrinsic Disorder. 1. Biological Processes
Functional Anthology of Intrinsic Disorder. 1. Biological Processes

...  2007 American Chemical Society ...
Session 209 Lens development and cell biology
Session 209 Lens development and cell biology

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Leukaemia Section t(2;9)(p23;q33) TRAF1/ALK Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(2;9)(p23;q33) TRAF1/ALK Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Genetic Analysis of the Large Subunit of Yeast Transcription Factor
Genetic Analysis of the Large Subunit of Yeast Transcription Factor

... and TFA1-391540 (described above), and cloned into the BamHI-HindIII backbone of pZM41, an integrating vector with a URA3 selectable marker. The PCR-amplified TFA1 fragment contains a BamHI restriction site just upstream of the TFA1 ATG, a BglII restriction site 183 bp downstream of the ATG, and a H ...
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Ch 15 Clicker Question

... B. Use RT-PCR to measure what proteins are present. C. Use a radioactive probe that binds only to certain mRNAs. D. Use a fluorescent probe that records DNA replication. E. Use a fluorescent probe that binds to only certain mRNAs. ...
Uniprot - European Bioinformatics Institute
Uniprot - European Bioinformatics Institute

... There is experimental evidence of the existence of a protein (e.g. Edman sequencing, MS, X-ray/NMR structure, good quality protein-protein interaction , detection by antibodies) ...
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PPT - Stanford University
PPT - Stanford University

... New genes and proteins are constantly being discovered, so explicitly enumerating and searching against a list of known entities is not scalable. Part of the difficulty lies in identifying previously unseen entities based on contextual, orthographic, and other clues. ...
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The Bacillus subtilis lipoprotein LplA causes cell lysis

... in the case of pAE-1, only 1 3 % of the population remained showing the ‘ amylolytic phenotype ’. These results indicated that the E. coli lysis phenotype was related to the expression of the foreign gene, and that the higher the number of copies of the gene the more toxic the phenotype was. During ...
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... essential bacterial targets in novel pathways, i.e. not targeted by currently used antibiotics, or novel targets in existing pathways. Promising targets for novel antibacterials against S. aureus include cell division, DNA replication and biosynthesis of fatty acid, peptidoglycan and protein [7]. To ...
pGLO Transformation and Green Fluorescent Protein - Bio-Rad
pGLO Transformation and Green Fluorescent Protein - Bio-Rad

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The NF1 Locus Encodes a Protein Functionally
The NF1 Locus Encodes a Protein Functionally

... Once the most highly conserved blocks corresponding to the GAP catalytic domain were mapped using MACAW, these short, approximately equal subsequences were extracted for an optimal global alignment using the MSA program (Lipman et al., 1989). The results are shown in Figure 2. The probability that b ...
Glycogen Slides from Class
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... When exercising- that glc is taken up by muscle and used to make more pyruvate/lactate and cycle continues When stop exercising- muscle cells stop taking up as much glc- thus liver glc conc goes up- this serves as signal to decrease glycogen breakdown. ...
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... 2. The ribosome helps form a polypeptide bond between the amino acids. 3. The ribosome pulls the mRNA strand the length of one codon and a new tRNA binds ...
Full Text
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... INTRODUCTION Discrete protein sequence motifs are widely used to describe homology between proteins and establish relationships between well-known and new protein sequences. More specifically, discrete motifs identify amino acids sharing important properties conserved in evolution. Further, they are ...
Plastid-Targeting Peptides from the
Plastid-Targeting Peptides from the

... (allowing entry in to the endomembrane system) and a chloroplast transit peptide (for transport across the chloroplast envelope membranes). We have examined the leader sequences from 45 full-length predicted plastid-targeted proteins from the chlorarachniophyte Bigelowiella natans with the goal of u ...
Macromolecule - Cloudfront.net
Macromolecule - Cloudfront.net

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Longins and their longin domains: regulated SNAREs and
Longins and their longin domains: regulated SNAREs and

... regulation of intracellular trafficking, such as subunits of the adaptor and transport protein particle complexes, also have LD-like folds. The functions and cellular localization of longins are regulated at several levels and the longin prototypes TI-VAMP, Sec22 and Ykt6 show different distribution ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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