Slide 1
... satisfy their own hydrogen-bonding groups. Transmembrane a-helices: Some transmembrane proteins have alpha-helices that are sufficiently long to span the membrane. The outer surfaces of these helices interact with the lipid core of the membrane. Transmembrane b-strands: Membranes can also be spanned ...
... satisfy their own hydrogen-bonding groups. Transmembrane a-helices: Some transmembrane proteins have alpha-helices that are sufficiently long to span the membrane. The outer surfaces of these helices interact with the lipid core of the membrane. Transmembrane b-strands: Membranes can also be spanned ...
Cover Page The handle http://hdl.handle.net/41480 holds
... tool and this can be done on an organism or cellular scale. Investigation of localisation is particularly important for study of development in multicellular organisms and as an indicator of protein function in single cells. Ideally, measurement of expression is done by detecting the final gene prod ...
... tool and this can be done on an organism or cellular scale. Investigation of localisation is particularly important for study of development in multicellular organisms and as an indicator of protein function in single cells. Ideally, measurement of expression is done by detecting the final gene prod ...
A Few Axonal Proteins Distinguish Ventral Spinal Cord Neurons
... system . The distinctive identity of individual classes of neurons has been well defined electrophysiologically, morphologically, and with respect to presumptive neurotransmitters, but less so with regard to their distinctive molecular composition . The probes for macromolecular differences between ...
... system . The distinctive identity of individual classes of neurons has been well defined electrophysiologically, morphologically, and with respect to presumptive neurotransmitters, but less so with regard to their distinctive molecular composition . The probes for macromolecular differences between ...
Characterizing Protein/Ligand Binding by DSC
... binding specific molecules such as other proteins, cofactors, prosthetic groups or drugs. Efforts to understand the mechanisms controlling selective binding were initially prompted by the realization that recognition and binding are universal features of all biochemical processes. These efforts have ...
... binding specific molecules such as other proteins, cofactors, prosthetic groups or drugs. Efforts to understand the mechanisms controlling selective binding were initially prompted by the realization that recognition and binding are universal features of all biochemical processes. These efforts have ...
PPT - Bioinformatics.ca
... – Signal peptidase cleavage site, consensus sequence for secretion extracellular – Address printed neatly, postal code ...
... – Signal peptidase cleavage site, consensus sequence for secretion extracellular – Address printed neatly, postal code ...
Hyperproduction of Exocellular Levansucrase by
... quantitative approach were the following. For strain 168, n = 2.5 0.2 and K = 1.1 mM", and for strain QB 112. n = 2.3 0.1 and K = 1-2 mM". The concentration of inducer for halfmaximum induction was 12.0 mM in both strains. The value of n shows that more than two molecules of sucrose are necessary to ...
... quantitative approach were the following. For strain 168, n = 2.5 0.2 and K = 1.1 mM", and for strain QB 112. n = 2.3 0.1 and K = 1-2 mM". The concentration of inducer for halfmaximum induction was 12.0 mM in both strains. The value of n shows that more than two molecules of sucrose are necessary to ...
From molecular activities and processes to biological function
... Figure 1. This also illustrates the flexibility of our description, as classes can be readily made more general or more specific in order to represent information on different types of biological processes, which may not necessarily be at the molecular level. It should be realised that the informati ...
... Figure 1. This also illustrates the flexibility of our description, as classes can be readily made more general or more specific in order to represent information on different types of biological processes, which may not necessarily be at the molecular level. It should be realised that the informati ...
GcvA, a LysR-type transcriptional regulator protein
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
Enzymes - africangreyparrott.com
... A. This enzyme works best at a temp. of 35 C and a pH of 8. B. This enzyme works best at a temp. of 50 C and a pH of 12. C. Temp. and pH have no influence on the activity of this enzyme. D. This enzyme works best at a temp. above 50 C and a pH above ...
... A. This enzyme works best at a temp. of 35 C and a pH of 8. B. This enzyme works best at a temp. of 50 C and a pH of 12. C. Temp. and pH have no influence on the activity of this enzyme. D. This enzyme works best at a temp. above 50 C and a pH above ...
Diapositivo 1
... Transferrin receptor and ferritin are regulated by different types of mechanisms, their levels respond oppositely to iron concentrations even though they are regulated by the same iron-responsive regulatory protein ...
... Transferrin receptor and ferritin are regulated by different types of mechanisms, their levels respond oppositely to iron concentrations even though they are regulated by the same iron-responsive regulatory protein ...
Molecular Structures Shared by Prokaryotes
... However, upon closer examination, many protein sequences which are involved in these structures and processes shared by prokaryotes and eukaryotes show low sequence homology and are similar in structure only. Therefore, these proteins can only be said to be analogous to each other, rather than homol ...
... However, upon closer examination, many protein sequences which are involved in these structures and processes shared by prokaryotes and eukaryotes show low sequence homology and are similar in structure only. Therefore, these proteins can only be said to be analogous to each other, rather than homol ...
Rabphilin mutants defective for Rab3 binding
... constructs attached on glutathione-Sepharose beads with extracts of PC12 cells. GST-GFP (a fusion protein between GST and the Green Fluorescent Protein) was used as a negative control. Because Rabphilin interacts preferentially with the GTP-bound form of Rab3A, the PC12 extracts were incubated with ...
... constructs attached on glutathione-Sepharose beads with extracts of PC12 cells. GST-GFP (a fusion protein between GST and the Green Fluorescent Protein) was used as a negative control. Because Rabphilin interacts preferentially with the GTP-bound form of Rab3A, the PC12 extracts were incubated with ...
PDF
... are prime candidates for reference species, since these are the organisms for which the molecular mechanisms of development are known in most detail. Both these species fall within the Ecdysozoa, and thus represent just one of the three clades of bilaterian animals defined by recent molecular phylog ...
... are prime candidates for reference species, since these are the organisms for which the molecular mechanisms of development are known in most detail. Both these species fall within the Ecdysozoa, and thus represent just one of the three clades of bilaterian animals defined by recent molecular phylog ...
G Protein Linked Receptors
... neurotransmitters, and local mediators, which are as varied in structure as they are in function: the list includes proteins and small peptides, as well as amino acid and fatty acid derivatives. • The same ligand can activate many different family members. • At least 9 distinct G-protein-linked rece ...
... neurotransmitters, and local mediators, which are as varied in structure as they are in function: the list includes proteins and small peptides, as well as amino acid and fatty acid derivatives. • The same ligand can activate many different family members. • At least 9 distinct G-protein-linked rece ...
Agrobacterium Rhizogeneze
... of virE2? Is the Galls gene regulated by virA/virG and phenolic compounds and sugars released by wounded plant cells?. Galls protein will help us learn more about gene transfer to plants. ...
... of virE2? Is the Galls gene regulated by virA/virG and phenolic compounds and sugars released by wounded plant cells?. Galls protein will help us learn more about gene transfer to plants. ...
Rearrangements of the Blood Group RhD Gene
... which is composed oftwo homologous structural genes, D and CcEe, that have been recently cloned."6The genome of Rh-positive and Rh-negative individuals can be distinguished, becausethe former carry two genes(Dand CcEe) and the latter only one ( C C E ~The ) . ~D gene encodes a multispanning membrane ...
... which is composed oftwo homologous structural genes, D and CcEe, that have been recently cloned."6The genome of Rh-positive and Rh-negative individuals can be distinguished, becausethe former carry two genes(Dand CcEe) and the latter only one ( C C E ~The ) . ~D gene encodes a multispanning membrane ...
NO!!!!!
... In eukaryotes, protein sorting or protein targeting is the process of directing proteins to distinct organelles such as the nucleus, mitochondria, and endoplasmic reticulum, or directing them out out of the cell. Two pathways are used to sort proteins. In one, completed proteins are synthesized in t ...
... In eukaryotes, protein sorting or protein targeting is the process of directing proteins to distinct organelles such as the nucleus, mitochondria, and endoplasmic reticulum, or directing them out out of the cell. Two pathways are used to sort proteins. In one, completed proteins are synthesized in t ...
Membranes
... The secretory pathway (3) 1) All secreted, ER-resident, lysosomal proteins, and many TM proteins contain a signal peptide at the N-term 13-36 Aa, hydrophobic 2) This signal peptide is recognized by the signal recognition particle, while the protein is being made by the ribosome, translational arr ...
... The secretory pathway (3) 1) All secreted, ER-resident, lysosomal proteins, and many TM proteins contain a signal peptide at the N-term 13-36 Aa, hydrophobic 2) This signal peptide is recognized by the signal recognition particle, while the protein is being made by the ribosome, translational arr ...
Comparative Analysis of DNA-Binding Proteins between
... The best studied cell components of thermophiles are proteins [9]. Proteins from thermophiles have characteristic amino acid compositions compared with mesophiles [3, 14]. That is mainly because of the differences in composition on the protein surface [3]. The surface regions of thermophilic protein ...
... The best studied cell components of thermophiles are proteins [9]. Proteins from thermophiles have characteristic amino acid compositions compared with mesophiles [3, 14]. That is mainly because of the differences in composition on the protein surface [3]. The surface regions of thermophilic protein ...
Cosmids, phasmids and other advanced vectors
... promoter specific to the vector. Such vector-carried promoters have been optimized for binding of the E. coli RNA polymerase and many of them can be regulated easily by changes in the growth conditions of the host cell. E. coli RNA polymerase is a multi-subunit enzyme. The core enzyme consists of tw ...
... promoter specific to the vector. Such vector-carried promoters have been optimized for binding of the E. coli RNA polymerase and many of them can be regulated easily by changes in the growth conditions of the host cell. E. coli RNA polymerase is a multi-subunit enzyme. The core enzyme consists of tw ...
Genetic Techniques for Biological Research Case study II
... the parents and the resulting diploid. (c) For the cross in part (a), show the results of tetrad analysis of the diploid. ...
... the parents and the resulting diploid. (c) For the cross in part (a), show the results of tetrad analysis of the diploid. ...
Leukaemia Section t(14;19)(q32;q13) IGH/CEBPA Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Alternatively, CEBPG can be involved instead of CEBPA (one case so far described). It is unknown if they bear the same prognosis, as they differ in their Nterm. DNA/RNA CEBPA is a single-exon gene, CEBPG also. Protein DNA-binding protein. CCAAT enhancer-binding protein (CEBP) transcription factors a ...
... Alternatively, CEBPG can be involved instead of CEBPA (one case so far described). It is unknown if they bear the same prognosis, as they differ in their Nterm. DNA/RNA CEBPA is a single-exon gene, CEBPG also. Protein DNA-binding protein. CCAAT enhancer-binding protein (CEBP) transcription factors a ...
Journal club
... • One of these, wrbA, gives growth rates nearly the same on cellobiose as on glucose (and there is no added cellobiose transporter, so must be using a glucose transporter OK – he never considers transport!) • Did the same with the xylosidase gly43F, but here other promoters were better, while none g ...
... • One of these, wrbA, gives growth rates nearly the same on cellobiose as on glucose (and there is no added cellobiose transporter, so must be using a glucose transporter OK – he never considers transport!) • Did the same with the xylosidase gly43F, but here other promoters were better, while none g ...
Rotate into shape: MreB and bacterial
... MreB, the bacterial actin homologue, plays a vital role in determining cell shape, but the mechanisms by which it actually functions have remained largely mysterious. Recent studies now shed new light on MreB, demonstrating that it associates with many cell-wall synthesis enzymes, including a newly ...
... MreB, the bacterial actin homologue, plays a vital role in determining cell shape, but the mechanisms by which it actually functions have remained largely mysterious. Recent studies now shed new light on MreB, demonstrating that it associates with many cell-wall synthesis enzymes, including a newly ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.