Leukaemia Section t(2;3)(p23;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Anaplasic large cell lymphoma: translocations involving 2p23 are found in more than half cases of anaplasic large cell lymphoma (ALCL), a high grade non Hodgkin lymphoma (NHL). They involve ALK, and are therefore called ALK+ ALCL. The most frequent ALK+ ALCL being the the t(2;5)(p23;q35) with NPM1 - ...
... Anaplasic large cell lymphoma: translocations involving 2p23 are found in more than half cases of anaplasic large cell lymphoma (ALCL), a high grade non Hodgkin lymphoma (NHL). They involve ALK, and are therefore called ALK+ ALCL. The most frequent ALK+ ALCL being the the t(2;5)(p23;q35) with NPM1 - ...
Functions of the Rumen
... • Once simple sugars are formed, they are absorbed rapidly by small intestine • Then monosaccharides diffuse into the portal vein which transports them to sites of metabolism ...
... • Once simple sugars are formed, they are absorbed rapidly by small intestine • Then monosaccharides diffuse into the portal vein which transports them to sites of metabolism ...
corneagene_cm
... for extensive biochemical studies and does not allow in vivo investigations. To overcome this problem, we plan to establish immortalized epithelial cell lines from dystrophic and normal corneas. The immortalization is done with viral or plasmid vectors, containing SV40 viral sequences. The establis ...
... for extensive biochemical studies and does not allow in vivo investigations. To overcome this problem, we plan to establish immortalized epithelial cell lines from dystrophic and normal corneas. The immortalization is done with viral or plasmid vectors, containing SV40 viral sequences. The establis ...
The Plant Journal
... Calmodulin is a regulatory protein activated during Ca2+ signalling. We have isolated a cDNA, designated LeCBDGK (Lycopersicon esculentum calmodulin-binding diacylglycerol kinase) encoding a novel calmodulin-binding protein with sequence similarity to diacylglycerol kinases from animals. Diacylglyce ...
... Calmodulin is a regulatory protein activated during Ca2+ signalling. We have isolated a cDNA, designated LeCBDGK (Lycopersicon esculentum calmodulin-binding diacylglycerol kinase) encoding a novel calmodulin-binding protein with sequence similarity to diacylglycerol kinases from animals. Diacylglyce ...
Acute Phase Proteins - a Potent Biomarker for Mastitis
... Several plasma proteins are known as APPs, however, depending on the species the protein pattern of each single APP during the APR is highly variable. In cattle, Hp and serum amyloid A (SAA) are considered as the most prominent APPs, whereas C-reactive protein (CRP) is normally present in circulatio ...
... Several plasma proteins are known as APPs, however, depending on the species the protein pattern of each single APP during the APR is highly variable. In cattle, Hp and serum amyloid A (SAA) are considered as the most prominent APPs, whereas C-reactive protein (CRP) is normally present in circulatio ...
Passage 36
... Protein synthesis begins when the gene encoding a protein is activated. The gene’s sequence of nucleotides is transcribed into a molecule of messenger RNA (mRNA), which reproduces the information contained in that (5) sequence. Transported outside the nucleus to the cytoplasm, the mRNA is translated ...
... Protein synthesis begins when the gene encoding a protein is activated. The gene’s sequence of nucleotides is transcribed into a molecule of messenger RNA (mRNA), which reproduces the information contained in that (5) sequence. Transported outside the nucleus to the cytoplasm, the mRNA is translated ...
Translation I
... Fig. 3. Interactions between tyrosyl-tRNA synthetase and tRNAtyr. (A) The C-terminal domain (orange) binds in the elbow between the long variable arm and the anti-codon stem of the tRNA (red backbone, green bases). The anti-codon stem loop interacts with both the C-terminal domain and the -helical d ...
... Fig. 3. Interactions between tyrosyl-tRNA synthetase and tRNAtyr. (A) The C-terminal domain (orange) binds in the elbow between the long variable arm and the anti-codon stem of the tRNA (red backbone, green bases). The anti-codon stem loop interacts with both the C-terminal domain and the -helical d ...
Chapter 17 notes
... Eukaryotic RNA processing • Eukaryotic mRNA receives a modified GTP cap at the 5’ end and a poly-A tail (stretch of nucleotides) at the 3’ end (protects from degradation & enhance translation) • 30 – 200 adenine nucleotides (poly–A) ...
... Eukaryotic RNA processing • Eukaryotic mRNA receives a modified GTP cap at the 5’ end and a poly-A tail (stretch of nucleotides) at the 3’ end (protects from degradation & enhance translation) • 30 – 200 adenine nucleotides (poly–A) ...
Yeast and Genetic Studies
... You now have a collection of haploid mutants that each carry only one mutation. You might begin by asking the following questions: 1. Is the phenotype of each mutant dominant or recessive? 2. Which of the mutants have mutations in the same gene? 3. Which of the mutants have mutations in different ge ...
... You now have a collection of haploid mutants that each carry only one mutation. You might begin by asking the following questions: 1. Is the phenotype of each mutant dominant or recessive? 2. Which of the mutants have mutations in the same gene? 3. Which of the mutants have mutations in different ge ...
Signal Transduction From the Endoplasmic Reticulum to the Cell
... Recently, a consensus has been reached. It is now agreed that Hac1p production is regulated at the posttranscriptional level. After reinvestigating their experimental system, Chapman and Walter (15) now report that both forms of Hac1, the spliced 238-amino acid form and the unspliced 230-amino acid ...
... Recently, a consensus has been reached. It is now agreed that Hac1p production is regulated at the posttranscriptional level. After reinvestigating their experimental system, Chapman and Walter (15) now report that both forms of Hac1, the spliced 238-amino acid form and the unspliced 230-amino acid ...
2005-05_Purdue_edimmer
... Evaluation of precision of annotation electronic techniques (InterPro2GO, SPKW2GO, EC2GO) • Compared manually-curated test set of GO annotated proteins with the electronic annotations • InterPro2GO = most coverage • EC2GO = 67 % of predictions exactly match the manual GO annotation. • 91-100 % of t ...
... Evaluation of precision of annotation electronic techniques (InterPro2GO, SPKW2GO, EC2GO) • Compared manually-curated test set of GO annotated proteins with the electronic annotations • InterPro2GO = most coverage • EC2GO = 67 % of predictions exactly match the manual GO annotation. • 91-100 % of t ...
CHAPTER 3 LEARNING OBJECTIVES -
... Know the functions of the cell wall Know what the cell wall is made out of and its characteristics Peptidoglycan, which is strong, porous, and flexible Be able to describe the monomer of the cell wall Two sugars (NAM and NAG) with 4 unusual amino acids attached to NAM Understand the typ ...
... Know the functions of the cell wall Know what the cell wall is made out of and its characteristics Peptidoglycan, which is strong, porous, and flexible Be able to describe the monomer of the cell wall Two sugars (NAM and NAG) with 4 unusual amino acids attached to NAM Understand the typ ...
Gene Section MYST3 (MYST histone acetyltransferase (monocytic leukemia) 3
... Abnormal Protein The fusion product retains the zinc fingers, the HAT domain of MYST3 and most of CREBBP, including the CREBBP interacting domain and the HAT domain; the fusion protein may repress RUNX1-dependant gene ...
... Abnormal Protein The fusion product retains the zinc fingers, the HAT domain of MYST3 and most of CREBBP, including the CREBBP interacting domain and the HAT domain; the fusion protein may repress RUNX1-dependant gene ...
Structure of the Reovirus Membrane
... land et al., 2001), and this competition may be part of the mechanism whereby 1 counteracts the effects of 3 on PKR activation and translation (Tillotson and Shatkin, 1992). The Autolytic Cleavage Site The 1 subunits in the crystals have clearly undergone cleavage into 1N and 1C: the C␣ atoms o ...
... land et al., 2001), and this competition may be part of the mechanism whereby 1 counteracts the effects of 3 on PKR activation and translation (Tillotson and Shatkin, 1992). The Autolytic Cleavage Site The 1 subunits in the crystals have clearly undergone cleavage into 1N and 1C: the C␣ atoms o ...
“Silent Angels” – Modifying Rett Syndrome Gene
... performance and emotional control occur that progress rapidly. Motor function deficit is often characterized by twisting hands, rubbing hands together or even placing hands into the mouth. Rett Syndrome cannot be cured. So far, drugs can only alleviate the symptoms. The research team led by Dr. Lee ...
... performance and emotional control occur that progress rapidly. Motor function deficit is often characterized by twisting hands, rubbing hands together or even placing hands into the mouth. Rett Syndrome cannot be cured. So far, drugs can only alleviate the symptoms. The research team led by Dr. Lee ...
Application of a bacterial two-hybrid system for the
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
Ch 9 modified
... TGN sorting mechanisms are complex • Many different “cargo selection” mechanisms – PTM – Protein aggregation – Signal receptor – Lipid raft ...
... TGN sorting mechanisms are complex • Many different “cargo selection” mechanisms – PTM – Protein aggregation – Signal receptor – Lipid raft ...
PSI - European Bioinformatics Institute
... • Given a set of uncharacterised sequences, we usually want to know: ...
... • Given a set of uncharacterised sequences, we usually want to know: ...
DNA-Bound Fos Proteins Activate Transcription in Yeast
... and possess an affinity for DNA (Donner et al., 1982; Watt et al., 1985; Renz et al., 1987). One plausible idea for the Fos proteins is that they exert their effects by altering gene expression (see, for example, Varmus, 1987). Similarly, it is possible that Myc proteins might exert some of their ef ...
... and possess an affinity for DNA (Donner et al., 1982; Watt et al., 1985; Renz et al., 1987). One plausible idea for the Fos proteins is that they exert their effects by altering gene expression (see, for example, Varmus, 1987). Similarly, it is possible that Myc proteins might exert some of their ef ...
A novel protein transport system involved in the biogenesis of
... only invariant residue. We therefore think it unlikely that these proteins provide the signal peptide binding site. In contrast, TatC proteins show substantial sequence conservation, and so we have proposed that TatC proteins contain the site of signal peptide recognition [6]. Note that a single cop ...
... only invariant residue. We therefore think it unlikely that these proteins provide the signal peptide binding site. In contrast, TatC proteins show substantial sequence conservation, and so we have proposed that TatC proteins contain the site of signal peptide recognition [6]. Note that a single cop ...
Midterm 1 from 2009
... the same phenotype, you realize that you cannot carry out a standard epistasis test by making double mutants. However, you have antibodies directed against the protein products produced by each gene n through r. You use these antibodies to look at the corresponding protein distribution of proteins N ...
... the same phenotype, you realize that you cannot carry out a standard epistasis test by making double mutants. However, you have antibodies directed against the protein products produced by each gene n through r. You use these antibodies to look at the corresponding protein distribution of proteins N ...
ppt - University of Illinois Urbana
... structure patterns of the family members to reveal shared characteristics that potentially describe common biological properties – Multiple sequence alignment – Motif/Domain - sequence and/or structure patterns common to protein family members (a trait) – Profile is a way to represent a family of pr ...
... structure patterns of the family members to reveal shared characteristics that potentially describe common biological properties – Multiple sequence alignment – Motif/Domain - sequence and/or structure patterns common to protein family members (a trait) – Profile is a way to represent a family of pr ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.