Gene Hunting for the Cystic Fibrosis gene - CusMiBio
... In the following page, you find your cDNA sequence and, below, the alignment of this sequence to the genomic sequence. Matching bases in cDNA and genomic sequences are colored blue and capitalized. Light blue bases mark the boundaries of gaps in either sequence (often splice sites). In the genomic s ...
... In the following page, you find your cDNA sequence and, below, the alignment of this sequence to the genomic sequence. Matching bases in cDNA and genomic sequences are colored blue and capitalized. Light blue bases mark the boundaries of gaps in either sequence (often splice sites). In the genomic s ...
WWOX, a Novel WW Domain-containing Protein
... Interestingly, the NH2 terminus of the putative WWOX protein showed homology to ubiquitin ligases such as NEDD4, YES-associated protein YAP65, and other WW domain-containing proteins (data not shown). Further amino acid sequence analysis using the PROSITE database identified two regions within the p ...
... Interestingly, the NH2 terminus of the putative WWOX protein showed homology to ubiquitin ligases such as NEDD4, YES-associated protein YAP65, and other WW domain-containing proteins (data not shown). Further amino acid sequence analysis using the PROSITE database identified two regions within the p ...
Leukaemia Section t(X;11)(q24;q23) MLL -SEPTIN6 Atlas of Genetics and Cytogenetics
... patients in all cases occurred in the MLL 8.3 kb breakpoint cluster region (BCR) and seem to occur preferentially in the telomeric half (between introns 7 and 11) of the MLL BCR. In the majority of reported cases 5' MLL sequences joined in-frame with SEPTIN6 downstream of SEPT6 exon 1. In rare cases ...
... patients in all cases occurred in the MLL 8.3 kb breakpoint cluster region (BCR) and seem to occur preferentially in the telomeric half (between introns 7 and 11) of the MLL BCR. In the majority of reported cases 5' MLL sequences joined in-frame with SEPTIN6 downstream of SEPT6 exon 1. In rare cases ...
Document
... has hindered their characterization at the molecular level, the determination of factors involved in their pathogenicity and the discovery of effective ways to control or cure phytoplasma diseases. Analysis of the phytoplasma genome suggests reductive evolution as a consequence of its life as an int ...
... has hindered their characterization at the molecular level, the determination of factors involved in their pathogenicity and the discovery of effective ways to control or cure phytoplasma diseases. Analysis of the phytoplasma genome suggests reductive evolution as a consequence of its life as an int ...
Youngs, Noah: Progress in the Side-Chain Prediction Problem
... conformation for each residue, and then systematically move down through the lessfavorable rotamers until one is found that does not conflict with the given backbone. This procedure is bound to create clashes between side-chains, but these are simply shoved into interacting “clusters”, and resolved ...
... conformation for each residue, and then systematically move down through the lessfavorable rotamers until one is found that does not conflict with the given backbone. This procedure is bound to create clashes between side-chains, but these are simply shoved into interacting “clusters”, and resolved ...
Slide 1
... Why Site directed mutagenesis -> site-directed mutagenesis -> point mutations in particular known area result -> library of wild-type and mutated DNA (sitespecific) not really a library -> just 2 species ...
... Why Site directed mutagenesis -> site-directed mutagenesis -> point mutations in particular known area result -> library of wild-type and mutated DNA (sitespecific) not really a library -> just 2 species ...
Synthetic macronutrients (for example, amino acids, which are
... Terry Shistar, Ph.D. Board of Directors ...
... Terry Shistar, Ph.D. Board of Directors ...
supplement
... Rabbit anti-ETO was used at the same concentration as previously described (1); Rabbit anti-Nvy antisera was generated against GST-tagged, Nvy expressed and purified from E. coli (see Supplemental methods). Pre-absorbed rabbit anti-Nvy was used at 1:300; rat anti-Elav 7E8A10 (Hybridoma Bank) was use ...
... Rabbit anti-ETO was used at the same concentration as previously described (1); Rabbit anti-Nvy antisera was generated against GST-tagged, Nvy expressed and purified from E. coli (see Supplemental methods). Pre-absorbed rabbit anti-Nvy was used at 1:300; rat anti-Elav 7E8A10 (Hybridoma Bank) was use ...
Alternative Splicing
... • familial dysautonomia results from a singlenucleotide mutation that causes a gene to be alternatively spliced in nervous system tissue • The decrease in the IKBKAP protein leads to abnormal nervous system development (half die before 30) • > 15% of gene mutations that cause genetic diseases and ca ...
... • familial dysautonomia results from a singlenucleotide mutation that causes a gene to be alternatively spliced in nervous system tissue • The decrease in the IKBKAP protein leads to abnormal nervous system development (half die before 30) • > 15% of gene mutations that cause genetic diseases and ca ...
Is central dogma a global property of cellular
... specifically, the central dogma describes the transfer of sequence information during DNA replication, transcription into RNA, and translation into amino-acid chains forming proteins. At the same time, it also states that information cannot flow from protein to protein or nucleic acid. Since the adv ...
... specifically, the central dogma describes the transfer of sequence information during DNA replication, transcription into RNA, and translation into amino-acid chains forming proteins. At the same time, it also states that information cannot flow from protein to protein or nucleic acid. Since the adv ...
Full Text - Science and Education Publishing
... Abstract The presence of ESBLs in many E. coli strains are of serious concern, since these organisms are the most common cause of different human infections. In this study we isolate an E. coli bacterium with high hydrolytic activity against cefotaxime. The ESBLs production was confirmed by phenotyp ...
... Abstract The presence of ESBLs in many E. coli strains are of serious concern, since these organisms are the most common cause of different human infections. In this study we isolate an E. coli bacterium with high hydrolytic activity against cefotaxime. The ESBLs production was confirmed by phenotyp ...
National Center for Biotechnology Information
... BLAST (Basic Local Alignment Search Tool) provides the functionality of allowing the user to input a protein or DNA sequence and performing a search for structures of similar sequence. Steps for performing a protein BLAST search will be detailed, however a DNA search is very much similar. From th ...
... BLAST (Basic Local Alignment Search Tool) provides the functionality of allowing the user to input a protein or DNA sequence and performing a search for structures of similar sequence. Steps for performing a protein BLAST search will be detailed, however a DNA search is very much similar. From th ...
PDF
... MBD4 interacts directly with DNMTs from various species. In mouse cells, MBD4 recruits MLH1 to methylated DNA sites that also contain DNMT1, and localised UV irradiation of nuclei triggers the recruitment of all three factors to sites of DNA damage. Based on these and other data, the authors suggest ...
... MBD4 interacts directly with DNMTs from various species. In mouse cells, MBD4 recruits MLH1 to methylated DNA sites that also contain DNMT1, and localised UV irradiation of nuclei triggers the recruitment of all three factors to sites of DNA damage. Based on these and other data, the authors suggest ...
Different subcellular locations of secretome components of
... Gram-positive bacteria contain different types of secretion systems for the transport of proteins into or across the cytoplasmic membrane. Recent studies on subcellular localization of specific components of these secretion systems and their substrates have shown that they can be present at various ...
... Gram-positive bacteria contain different types of secretion systems for the transport of proteins into or across the cytoplasmic membrane. Recent studies on subcellular localization of specific components of these secretion systems and their substrates have shown that they can be present at various ...
Otras redes en Biología Molecular - Computational Systems Biology
... cell-proliferation and cell-survival pathways, for example, without affecting glycogen metabolism, we would need multicomponent drugs to specifically inhibit these two downstream pathways rather than using a single AKT inhibitor. b | Redundant pathways can compensate for inhibition of another pathwa ...
... cell-proliferation and cell-survival pathways, for example, without affecting glycogen metabolism, we would need multicomponent drugs to specifically inhibit these two downstream pathways rather than using a single AKT inhibitor. b | Redundant pathways can compensate for inhibition of another pathwa ...
If there is time OR when we get to Cell Unit…
... dictionary that they use when translating RNA sequences into protein sequences. This dictionary exists in the form of a series of molecules called tRNA's. ...
... dictionary that they use when translating RNA sequences into protein sequences. This dictionary exists in the form of a series of molecules called tRNA's. ...
The role of structural disorder in cell cycle regulation, related clinical
... found in the N-terminal and middle regions of Knl1 [39], in varying numbers. The MELT motif is phosphorylated by the mitotic checkpoint kinase Msp1, and this modification is necessary for the recruitment of Bub1 and Bub3 to the kinetochore [46]. Bub1 and BubR1 binding localizes to the N terminus of ...
... found in the N-terminal and middle regions of Knl1 [39], in varying numbers. The MELT motif is phosphorylated by the mitotic checkpoint kinase Msp1, and this modification is necessary for the recruitment of Bub1 and Bub3 to the kinetochore [46]. Bub1 and BubR1 binding localizes to the N terminus of ...
Evolution in the Test Tube as a Means to Create Functional Enzymes
... Some time ago we developed a fundamentally new approach to asymmetric catalysis, specifically the directed evolution of enantioselective enzymes.1 It is based on the appropriate combination of gene mutagenesis/expression and high-throughput eescreening.2 The organocatalytic enzymes that we have stud ...
... Some time ago we developed a fundamentally new approach to asymmetric catalysis, specifically the directed evolution of enantioselective enzymes.1 It is based on the appropriate combination of gene mutagenesis/expression and high-throughput eescreening.2 The organocatalytic enzymes that we have stud ...
BIO00011C Cell and Developmental Biology
... influence the activity of the transcription initiation complex? (1 mark) ...
... influence the activity of the transcription initiation complex? (1 mark) ...
Atomic contacts in protein structures. A detailed analysis of atomic
... the protein. An important, but as yet unresolved question is which functional constraints exactly are imposed on a protein structure. Sequence and structure conservation patterns provide valuable hints in this respect, like the conservation of the structure in the catalytic site of an enzyme. Howeve ...
... the protein. An important, but as yet unresolved question is which functional constraints exactly are imposed on a protein structure. Sequence and structure conservation patterns provide valuable hints in this respect, like the conservation of the structure in the catalytic site of an enzyme. Howeve ...
Review #2
... What is alternative splicing? How frequent is this process? How many proteins can be made for one gene? Why does the DSCAM gene in Drosophila make so many proteins? What are the necessary parts of an intron? What are the two reactions that need to occur during splicing? What is trans-splicing? Where ...
... What is alternative splicing? How frequent is this process? How many proteins can be made for one gene? Why does the DSCAM gene in Drosophila make so many proteins? What are the necessary parts of an intron? What are the two reactions that need to occur during splicing? What is trans-splicing? Where ...
Document
... *Primary-Reponse defined as upregulated by LPS (100ng/mL) at 2h in the presence of cycloheximide (20mg/mL) fSchematic representation of gene promoters created by using Celera web-based genomic database, TESS promoter analysis software (http://www.cbil.upenn.edu/tess/) and the TRANSFAC transcription ...
... *Primary-Reponse defined as upregulated by LPS (100ng/mL) at 2h in the presence of cycloheximide (20mg/mL) fSchematic representation of gene promoters created by using Celera web-based genomic database, TESS promoter analysis software (http://www.cbil.upenn.edu/tess/) and the TRANSFAC transcription ...
Diapositiva 1 - Universitat de Lleida
... a similar function, but now appear in different species. • Homologous genes (genes with share evolutionary origin) have similar sequences. • Paralogs are evolutionarily related (share an origin) but no longer have the same function. • You can uncover either orthologs or paralogs through sequence ali ...
... a similar function, but now appear in different species. • Homologous genes (genes with share evolutionary origin) have similar sequences. • Paralogs are evolutionarily related (share an origin) but no longer have the same function. • You can uncover either orthologs or paralogs through sequence ali ...
CHAPTER TWO
... subunits (multiple polypeptide molecules, each called a 'monomer'). The arrangement of the monomers in the three-dimensional protein is the quaternary structure.20, 21 ...
... subunits (multiple polypeptide molecules, each called a 'monomer'). The arrangement of the monomers in the three-dimensional protein is the quaternary structure.20, 21 ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.