Genomics
... Fig. 1. Structure of six AtUGT85A genes and three AtGUS genes in Arabidopsis. (A) Structure of AtUGT85A1, 2, 3, 4, 6, and 7 on chromosome 1. Five AtUGT85A genes are clustered together in an 18-kb region on the left arm of chromosome 1. AtUGT85A4 is located at the end of left arm of chromosome 1. Ope ...
... Fig. 1. Structure of six AtUGT85A genes and three AtGUS genes in Arabidopsis. (A) Structure of AtUGT85A1, 2, 3, 4, 6, and 7 on chromosome 1. Five AtUGT85A genes are clustered together in an 18-kb region on the left arm of chromosome 1. AtUGT85A4 is located at the end of left arm of chromosome 1. Ope ...
CapraZyme by Mt. Capra "First in Enzyme Function" A complete
... fibrous nature of these foods pose digestive challenges for many people and are indigestible because humans do not product Alpha-Galactosidase required to break them down. Helps to eliminate bloating, cramping, and intestinal fermentation by the breakdown of these carbohydrates before they reach the ...
... fibrous nature of these foods pose digestive challenges for many people and are indigestible because humans do not product Alpha-Galactosidase required to break them down. Helps to eliminate bloating, cramping, and intestinal fermentation by the breakdown of these carbohydrates before they reach the ...
C-terminal EH-domain-containing proteins
... 1990). A recent study has demonstrated that ATP is the primary nucleotide that binds to and is hydrolyzed by EHD1 (Lee et al., 2005), although it remains possible that in vivo EHD1 might also be capable of binding and/or hydrolyzing GTP. The first study to demonstrate the functional significance of ...
... 1990). A recent study has demonstrated that ATP is the primary nucleotide that binds to and is hydrolyzed by EHD1 (Lee et al., 2005), although it remains possible that in vivo EHD1 might also be capable of binding and/or hydrolyzing GTP. The first study to demonstrate the functional significance of ...
Influence of residue 44 on the activity of the M2 proton channel of
... (Pinto et al., 1992; Chizhmakov et al., 1996; Mould et al., 2000) and is the specific target of the anti-influenza drugs amantadine and rimantadine. The M2 channel plays a role in the uncoating of influenza virions in endosomes (Martin & Helenius, 1991; Wharton et al., 1994). In addition, during inf ...
... (Pinto et al., 1992; Chizhmakov et al., 1996; Mould et al., 2000) and is the specific target of the anti-influenza drugs amantadine and rimantadine. The M2 channel plays a role in the uncoating of influenza virions in endosomes (Martin & Helenius, 1991; Wharton et al., 1994). In addition, during inf ...
CIP Posters - International Potato Center
... 1 that is highly similar (99%) to a double WRKY protein PPS8 of S. tuberosum, which is a candidate substrate for MAPKs that play pivotal roles in induced defence responses [6]. WRKY transcription factors are part of complex coregulatory mechanisms and more detailed expression studies are required to ...
... 1 that is highly similar (99%) to a double WRKY protein PPS8 of S. tuberosum, which is a candidate substrate for MAPKs that play pivotal roles in induced defence responses [6]. WRKY transcription factors are part of complex coregulatory mechanisms and more detailed expression studies are required to ...
6SULQJHU
... plants were transformed by in planta in®ltration (Bechthold et al. 1993). Seeds of in®ltrated plants were sown in soil and grown under continuous white light for 20 days. Plants were sprayed twice within 72 h with a solution of 0.1% glufosinate-ammonium (Agrevo) in 0.1% Tween 20. Tissues of glufosin ...
... plants were transformed by in planta in®ltration (Bechthold et al. 1993). Seeds of in®ltrated plants were sown in soil and grown under continuous white light for 20 days. Plants were sprayed twice within 72 h with a solution of 0.1% glufosinate-ammonium (Agrevo) in 0.1% Tween 20. Tissues of glufosin ...
The Bcl-3 oncoprotein acts as a bridging factor between NF
... cytoplasmic IkB proteins, IkBa, IkBb or IkBe (May and Ghosh, 1997). IkB-speci®c kinases IKKa and IKKb have been identi®ed that respond to extracellular signals and phosphorylate IkBa or IkBb (Maniatis, 1997; Stancovski and Baltimore, 1997). As a result, induced IkB degradation as well as p50 or p52 ...
... cytoplasmic IkB proteins, IkBa, IkBb or IkBe (May and Ghosh, 1997). IkB-speci®c kinases IKKa and IKKb have been identi®ed that respond to extracellular signals and phosphorylate IkBa or IkBb (Maniatis, 1997; Stancovski and Baltimore, 1997). As a result, induced IkB degradation as well as p50 or p52 ...
Subviral-Particle Biogenesis Hepatitis B Virus Small Surface
... Cells, transfection, Western blotting, and HBsAg assay. Huh7 cells were transiently transfected with Fugene 6/HD/X-treme (Roche, Penzberg, Germany) in 6-well plates using 1 g of plasmid DNA per well. At 2 days posttransfection, the culture supernatant was harvested and centrifuged for 10 min at 13, ...
... Cells, transfection, Western blotting, and HBsAg assay. Huh7 cells were transiently transfected with Fugene 6/HD/X-treme (Roche, Penzberg, Germany) in 6-well plates using 1 g of plasmid DNA per well. At 2 days posttransfection, the culture supernatant was harvested and centrifuged for 10 min at 13, ...
Ribosome locations
... site of biological protein synthesis (translation). Ribosomes link amino acids together in the order specified by messenger RNA (mRNA) molecules. Ribosomes consist of two major subunits—the small ribosomal subunit reads the mRNA, while the large subunit joins amino acids to form a polypeptide chain. ...
... site of biological protein synthesis (translation). Ribosomes link amino acids together in the order specified by messenger RNA (mRNA) molecules. Ribosomes consist of two major subunits—the small ribosomal subunit reads the mRNA, while the large subunit joins amino acids to form a polypeptide chain. ...
A Statistical Analysis of the Linear Interaction Energy Method
... • Aldose reductase - very flexible active site – FlexE docking successful (3 ligands) ...
... • Aldose reductase - very flexible active site – FlexE docking successful (3 ligands) ...
Gene Section GPC5 (glypican 5) Atlas of Genetics and Cytogenetics
... essential functions in cell growth and development (Burgess and Macaig, 1989; Andres et al., 1992). Glypicans appear to be expressed predominantly during development, with expression levels changing in a stage- and tissue-specific manner, suggesting their involvement in morphogenesis (Sing and Filmu ...
... essential functions in cell growth and development (Burgess and Macaig, 1989; Andres et al., 1992). Glypicans appear to be expressed predominantly during development, with expression levels changing in a stage- and tissue-specific manner, suggesting their involvement in morphogenesis (Sing and Filmu ...
npgrj_nmeth_1134 1..3
... The possibility to specifically regulate a protein of interest in extra- versus intracellular parasites is of importance when proteins with dual functions (for example during host cell invasion and intracellular development of the parasite) need to be analyzed. Next we inoculated parasites expressin ...
... The possibility to specifically regulate a protein of interest in extra- versus intracellular parasites is of importance when proteins with dual functions (for example during host cell invasion and intracellular development of the parasite) need to be analyzed. Next we inoculated parasites expressin ...
15.Flexible_Protein_Docking_Jonathan
... • Aldose reductase - very flexible active site – FlexE docking successful (3 ligands) ...
... • Aldose reductase - very flexible active site – FlexE docking successful (3 ligands) ...
The epidermal intermediate filament proteins of
... body preparations. Inclusion bodies were solubilised in 10 mM Na2HPO4, pH 6.6, containing 8 M urea and 1 mM 2-mercaptoethanol and subjected to cation exchange chromatography on Mono S 5/5 using a gradient formed by the solubilisation buffer and the same buffer containing 0.4 M NaCl. Elution profiles ...
... body preparations. Inclusion bodies were solubilised in 10 mM Na2HPO4, pH 6.6, containing 8 M urea and 1 mM 2-mercaptoethanol and subjected to cation exchange chromatography on Mono S 5/5 using a gradient formed by the solubilisation buffer and the same buffer containing 0.4 M NaCl. Elution profiles ...
ppt
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
Slide 1
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
π- Stacking Interaction
... • The agent is a misfolded form (PrS) of a normal cell protein (PrP) that acts by a conversion of normal folded PrP proteins to amyloid-like aggregates. • The importance of the octapeptide repeats is the fact result from the insertion of one to nine extra octapeptide repeats in addition to the five ...
... • The agent is a misfolded form (PrS) of a normal cell protein (PrP) that acts by a conversion of normal folded PrP proteins to amyloid-like aggregates. • The importance of the octapeptide repeats is the fact result from the insertion of one to nine extra octapeptide repeats in addition to the five ...
Slide 1
... Null mutations in the C. elegans heterochronic gene 21 nucleotide regulatory RNA. A lin-41::GFP fusion lin-41 cause precocious expression of adult fate at gene is downregulated in tissues affected in late lar- ...
... Null mutations in the C. elegans heterochronic gene 21 nucleotide regulatory RNA. A lin-41::GFP fusion lin-41 cause precocious expression of adult fate at gene is downregulated in tissues affected in late lar- ...
SECTION8PRACTICALANDDATASKILLS ms
... (Thus) changing amino acid sequence; Changes tertiary structure / shape of protein/transporter; Cocaine binding site changes/cocaine cannot bind; Dopamine can still bind (and be transported); 3 max ...
... (Thus) changing amino acid sequence; Changes tertiary structure / shape of protein/transporter; Cocaine binding site changes/cocaine cannot bind; Dopamine can still bind (and be transported); 3 max ...
The intracellular cyanobacteria of Paulinella chromatophora
... When interpreted within this kind of framework, emerging empirical data indicate that at least some modern endosymbionts are already well into such a transitional process. Peculiar endosymbionts or new organelles? Many insects harbor intracellular bacteria that produce and excrete essential amino ac ...
... When interpreted within this kind of framework, emerging empirical data indicate that at least some modern endosymbionts are already well into such a transitional process. Peculiar endosymbionts or new organelles? Many insects harbor intracellular bacteria that produce and excrete essential amino ac ...
Gene Section PRUNE (prune exopolyphosphatase) Atlas of Genetics and Cytogenetics
... Prune, a 62 kDa protein, belongs to the DHH family phosphoesterase proteins including RecJ DNA repair exonucleases, pyrophosphatases (PPASEs) and exopolyphospatases (PPX). The DHH super-family can be divided into two main groups on the basis of a Cterminal motif that is very well conserved within ea ...
... Prune, a 62 kDa protein, belongs to the DHH family phosphoesterase proteins including RecJ DNA repair exonucleases, pyrophosphatases (PPASEs) and exopolyphospatases (PPX). The DHH super-family can be divided into two main groups on the basis of a Cterminal motif that is very well conserved within ea ...
African Swine fever virus immune evasion genes
... This sequence was compared with that of 8 other virulent isolates and one non-virulent isolate which has been adapted to grow in tissue-culture cells. The analysis showed that most genes were present in the genomes of all of the different isolates. One feature of the ASFV genome is that 5 families o ...
... This sequence was compared with that of 8 other virulent isolates and one non-virulent isolate which has been adapted to grow in tissue-culture cells. The analysis showed that most genes were present in the genomes of all of the different isolates. One feature of the ASFV genome is that 5 families o ...
full lab details and projects
... ultrastructure arl13b mutants and genetic mosaic analysis to examine the long-term fate of mutant photoreceptors in wild type zebrafish. To determine relationship between Arl13b and PCP signaling, both genetic and physical interactions will be tested. Finally, the requirement for GTPase activity and ...
... ultrastructure arl13b mutants and genetic mosaic analysis to examine the long-term fate of mutant photoreceptors in wild type zebrafish. To determine relationship between Arl13b and PCP signaling, both genetic and physical interactions will be tested. Finally, the requirement for GTPase activity and ...
Gene Regulation - Mr. Kleiman`s Wiki
... If ingested levels of the amino acid tryptophan are low, e. coli is ableto synthesize it on its own. When tryptophan levels are low, the try operon is turned on to make the enzymes necessary to synthesize more. ...
... If ingested levels of the amino acid tryptophan are low, e. coli is ableto synthesize it on its own. When tryptophan levels are low, the try operon is turned on to make the enzymes necessary to synthesize more. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.