Nucleic Acids Research
... (3,4). Indeed, large patches of positive charges have been suggested to be characteristic of protein–nucleic acid interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface ( ...
... (3,4). Indeed, large patches of positive charges have been suggested to be characteristic of protein–nucleic acid interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface ( ...
![[BIO21] Biosynthetic production of human growth hormone](http://s1.studyres.com/store/data/003471523_1-f9bb77a7e4981f028d3ca33bef40615b-300x300.png)
[BIO21] Biosynthetic production of human growth hormone
... reverse many of the effects of aging (Klatz and Kahn, 1998). Besides 22K-hGH (22,000 Dalton), which is a major component composed of 191 amino acids, 20K-hGH (20,000 Dalton) is also known to be naturally secreted, being encoded by the same gene as 22K-hGH and lacking 15 amino acids (residues 32–46) ...
... reverse many of the effects of aging (Klatz and Kahn, 1998). Besides 22K-hGH (22,000 Dalton), which is a major component composed of 191 amino acids, 20K-hGH (20,000 Dalton) is also known to be naturally secreted, being encoded by the same gene as 22K-hGH and lacking 15 amino acids (residues 32–46) ...
Small and stable peptidic PEGylated quantum dots to - HAL
... In the present work, we report a simple alternative for labeling individual histag proteins with QDs while maintaining a small QD/protein complex size. We selected short peptides to stabilize the QDs in aqueous buffers because of their good chemical versatility as well as their higher chemical stabi ...
... In the present work, we report a simple alternative for labeling individual histag proteins with QDs while maintaining a small QD/protein complex size. We selected short peptides to stabilize the QDs in aqueous buffers because of their good chemical versatility as well as their higher chemical stabi ...
Gene Regulation - Mr. Kleiman`s Wiki
... If ingested levels of the amino acid tryptophan are low, e. coli is ableto synthesize it on its own. When tryptophan levels are low, the try operon is turned on to make the enzymes necessary to synthesize more. ...
... If ingested levels of the amino acid tryptophan are low, e. coli is ableto synthesize it on its own. When tryptophan levels are low, the try operon is turned on to make the enzymes necessary to synthesize more. ...
C2005/F2401 `07 -- Lecture 15 -- Last Edited
... a. Transcriptional control is common. It is the primary way, but not the only way, to regulate protein synthesis. b. Two part switches, consisting of a protein and DNA site are very, very common. The situation is often more complex than the one described above, especially in eukaryotes. There are of ...
... a. Transcriptional control is common. It is the primary way, but not the only way, to regulate protein synthesis. b. Two part switches, consisting of a protein and DNA site are very, very common. The situation is often more complex than the one described above, especially in eukaryotes. There are of ...
pdf
... high-level expression. For instance, for some genes the former could require that the strong negative effect of silencing chromatin be removed, whereas the latter could involve covalent modification of particular transcriptional activators. However, the full mechanistic details of both processes are ...
... high-level expression. For instance, for some genes the former could require that the strong negative effect of silencing chromatin be removed, whereas the latter could involve covalent modification of particular transcriptional activators. However, the full mechanistic details of both processes are ...
Precise Gene Expression: Proprietary RheoSwitch
... expression of mutant huntingtin protein in the development of an in vitro Huntington’s disease model.3 The ability to control expression enabled evaluation of the mutant protein’s effect on ...
... expression of mutant huntingtin protein in the development of an in vitro Huntington’s disease model.3 The ability to control expression enabled evaluation of the mutant protein’s effect on ...
Week 9
... Objective: Students will be introduced to the organic compounds and trace elements which make up a living organism and begin to gain an understanding of the organic compound carbohydrates. Activity: Five minute review on Activity: Pass out and work on Activity: Continue lecturing the specific carboh ...
... Objective: Students will be introduced to the organic compounds and trace elements which make up a living organism and begin to gain an understanding of the organic compound carbohydrates. Activity: Five minute review on Activity: Pass out and work on Activity: Continue lecturing the specific carboh ...
The role of protein–protein interactions in the intracellular traffic of
... network’ has been developed recently [37, 40]. (6) The binding of some interacting proteins depends on prior posttranslational modification of the target protein [99], e.g., phosphorylation [32, 91, 108]; this mechanism allows PPI to be regulated, e.g., via protein kinases. (7) Finally, it should be ...
... network’ has been developed recently [37, 40]. (6) The binding of some interacting proteins depends on prior posttranslational modification of the target protein [99], e.g., phosphorylation [32, 91, 108]; this mechanism allows PPI to be regulated, e.g., via protein kinases. (7) Finally, it should be ...
The Possible DNA-binding Nature of the Regulatory Proteins
... been extensively studied and the process has been divided into six stages on the basis of the appearance of cell sections in the electron microscope (Ryter et al., 1966). It is now known that the first two of these stages (0 and I) have, in fact, nothing to do with the developmental process and can ...
... been extensively studied and the process has been divided into six stages on the basis of the appearance of cell sections in the electron microscope (Ryter et al., 1966). It is now known that the first two of these stages (0 and I) have, in fact, nothing to do with the developmental process and can ...
Microfilaments Intermediate Filaments Extracellular Matrix
... • Its diameter is 8-12 nanometers in length • Also help make up the cytoskeleton along with microfilaments and microtubules. • There are 5 different types • But always include Keratin, a fibrous protein ...
... • Its diameter is 8-12 nanometers in length • Also help make up the cytoskeleton along with microfilaments and microtubules. • There are 5 different types • But always include Keratin, a fibrous protein ...
Powerpoint document
... • What is the intersection of my interaction set with all known published interactions? ...
... • What is the intersection of my interaction set with all known published interactions? ...
Genetics 314 - Spring, 2006
... non-sister chromatids in meiosis but not in mitosis. 3. The chemical division calls you to tell you they have found a chemical that induces transposable element movement producing deletions, duplications and inversions during post-meiotic mitotic divisions. They want to know if the impact of this ch ...
... non-sister chromatids in meiosis but not in mitosis. 3. The chemical division calls you to tell you they have found a chemical that induces transposable element movement producing deletions, duplications and inversions during post-meiotic mitotic divisions. They want to know if the impact of this ch ...
Qproteome Nuclear Protein Handbook
... binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s genetic information and is the site of gene expression. Biological processes involvin ...
... binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s genetic information and is the site of gene expression. Biological processes involvin ...
Solubilization of Membrane Proteins into Functional Lipid‐Bilayer
... the rim of the nanodiscs. In sharp contrast with the situation encountered in SMALPs, the transition temperature was not downshifted upon solubilization by moderate DIBMA concentrations (Figure 3 d). This suggests much less perturbation of lipid packing by DIBMA compared with SMA(3:1), the stronger ...
... the rim of the nanodiscs. In sharp contrast with the situation encountered in SMALPs, the transition temperature was not downshifted upon solubilization by moderate DIBMA concentrations (Figure 3 d). This suggests much less perturbation of lipid packing by DIBMA compared with SMA(3:1), the stronger ...
Leukaemia Section t(5;12)(q31;p13) in MDS, AML and AEL in Oncology and Haematology
... Cools J, Mentens N, Odero MD, Peeters P, Wlodarska I, Delforge M, Hagemeijer A, Marynen P. Evidence for position effects as a variant ETV6-mediated leukemogenic mechanism in myeloid leukemias with a t(4;12)(q11-q12;p13) or t(5;12)(q31;p13). Blood 2002;99:1776-1784. ...
... Cools J, Mentens N, Odero MD, Peeters P, Wlodarska I, Delforge M, Hagemeijer A, Marynen P. Evidence for position effects as a variant ETV6-mediated leukemogenic mechanism in myeloid leukemias with a t(4;12)(q11-q12;p13) or t(5;12)(q31;p13). Blood 2002;99:1776-1784. ...
tnf-alpha stimulated activation of mmp
... sites for Egr-1 can regulate gene expression [4]. In NP tissues, TNF induced a rapid and transient increase in Egr-1 mRNA by 0.5h which returned to baseline following 8h. A corresponding increase in levels of Egr-1 protein was detected, which peaked by 2h of TNF treatment and returned to basal level ...
... sites for Egr-1 can regulate gene expression [4]. In NP tissues, TNF induced a rapid and transient increase in Egr-1 mRNA by 0.5h which returned to baseline following 8h. A corresponding increase in levels of Egr-1 protein was detected, which peaked by 2h of TNF treatment and returned to basal level ...
Dynamic Proteomics of Individual Cancer Cells in Response to a
... variability in their fluorescence levels, with a standard deviation between cells that ranged between 10 and 60% of the mean. This variability is in accord with that previously found in microorganisms (2, 29–32) and human cells (12). Part of this variability is due to differences in the cell-cycle s ...
... variability in their fluorescence levels, with a standard deviation between cells that ranged between 10 and 60% of the mean. This variability is in accord with that previously found in microorganisms (2, 29–32) and human cells (12). Part of this variability is due to differences in the cell-cycle s ...
Lecture -18 Modification of food plant taste and appearance
... cauliflower mosaic virus. Each construct used the transcription terminationpolyadenylation site from a Ti plasmid nopaline synthase gene. In each case, the synthetic monellin gene was introduced into plant cells by A. tumefaciens infection, using the Ti plasmid cointegrate vector system. Monellin wa ...
... cauliflower mosaic virus. Each construct used the transcription terminationpolyadenylation site from a Ti plasmid nopaline synthase gene. In each case, the synthetic monellin gene was introduced into plant cells by A. tumefaciens infection, using the Ti plasmid cointegrate vector system. Monellin wa ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... division, we examined the effects of both FtsZ proteins by overproducing them in E. coli M15 by employing the expression vector PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ ...
... division, we examined the effects of both FtsZ proteins by overproducing them in E. coli M15 by employing the expression vector PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ ...
Operons: The Basic Concept
... (b) Lactose present, repressor inactive, operon on. Allolactose, an isomer of lactose, derepresses the operon by inactivating the repressor. In this way, the enzymes for lactose utilization are induced. Figure 18.22b ...
... (b) Lactose present, repressor inactive, operon on. Allolactose, an isomer of lactose, derepresses the operon by inactivating the repressor. In this way, the enzymes for lactose utilization are induced. Figure 18.22b ...
Applied and Environmental Microbiology
... construction of specialized bacterial strains for agricultural and industrial purposes. Of the bacterial species which have potential as enhancers of plant growth, some fluorescent pseudomonads show particular promise (24). This plant growth promotion requires efficient colonization of the plant roo ...
... construction of specialized bacterial strains for agricultural and industrial purposes. Of the bacterial species which have potential as enhancers of plant growth, some fluorescent pseudomonads show particular promise (24). This plant growth promotion requires efficient colonization of the plant roo ...
Leukaemia Section t(3;9)(q26;p23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0309q26p23ID1279.html DOI: 10.4267/2042/38531 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0309q26p23ID1279.html DOI: 10.4267/2042/38531 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.