Robert Jones
... Autoradiography with 125I-alpha-bungarotoxin (Bgt) and 3H-methyllycaconitine were used to map the general distribution of alpha7 nAChR within the rat PFC. AlexaFluor 488-conjugated Bgt in conjunction with other neuronal markers provided further analysis of regions of interest at higher magnificatio ...
... Autoradiography with 125I-alpha-bungarotoxin (Bgt) and 3H-methyllycaconitine were used to map the general distribution of alpha7 nAChR within the rat PFC. AlexaFluor 488-conjugated Bgt in conjunction with other neuronal markers provided further analysis of regions of interest at higher magnificatio ...
Chromosome segregation: Samurai separation
... regulate separation of sister chromatids. To test this hypothesis, a version of Scc1 that could not be cleaved was generated and tested in vivo [19]. First, the site of cleavage was identified by sequencing the carboxy-terminal fragment of Scc1. An arginine residue immediately upstream of the first ...
... regulate separation of sister chromatids. To test this hypothesis, a version of Scc1 that could not be cleaved was generated and tested in vivo [19]. First, the site of cleavage was identified by sequencing the carboxy-terminal fragment of Scc1. An arginine residue immediately upstream of the first ...
The central dogma: from gene sequence to molecular biology function
... This shows how the protein is translated. Each line contains the DNA sequence and highlights the threeletter codon along with the corresponding amino acid. If we look at the sequences labeled 5’3’ Frame 2 we will see that ‘g’ is not used, ‘ata’ translates to ‘I’, ‘aca translates to ‘T’, aag transla ...
... This shows how the protein is translated. Each line contains the DNA sequence and highlights the threeletter codon along with the corresponding amino acid. If we look at the sequences labeled 5’3’ Frame 2 we will see that ‘g’ is not used, ‘ata’ translates to ‘I’, ‘aca translates to ‘T’, aag transla ...
Cell Communication
... regulation of one or more cellular activities response may occur in cytoplasm or may involve action in nucleus ...
... regulation of one or more cellular activities response may occur in cytoplasm or may involve action in nucleus ...
Publications_files/Stotland et al 2012
... FACS-sorted, clonally selected on the basis of high mCherry expression, and probed with anti-CSN1 antibody to confirm the expression of SBP-CSN1 (Fig .1B–D). As a control, we utilized a similar construct where CSN1 was substituted with Citrine, a modified yellow fluorescent protein chosen to exclude ...
... FACS-sorted, clonally selected on the basis of high mCherry expression, and probed with anti-CSN1 antibody to confirm the expression of SBP-CSN1 (Fig .1B–D). As a control, we utilized a similar construct where CSN1 was substituted with Citrine, a modified yellow fluorescent protein chosen to exclude ...
Solid state NMR assignment of a whole virus particle
... The helix is assumed to have a kink in the center, or to be gently curved the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alter ...
... The helix is assumed to have a kink in the center, or to be gently curved the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alter ...
Methods in Molecular Biology
... of analysis of the TLR down-stream signal transduction pathways. Key intermediates of the signaling pathways are identified after expression in bacteria, by liquid chromatography and tandem mass spectrometry (LC-SM/SM) or by differential in-gel electrophoresis DIGE (2D electrophoresis coupled with p ...
... of analysis of the TLR down-stream signal transduction pathways. Key intermediates of the signaling pathways are identified after expression in bacteria, by liquid chromatography and tandem mass spectrometry (LC-SM/SM) or by differential in-gel electrophoresis DIGE (2D electrophoresis coupled with p ...
Which amino acids matter? - Berkeley Cosmology Group
... - We will measure the rate of reaction of each enzyme. - If the reaction rate is slower for a mutant, then it shows that there is a major effect if we change that particular amino acid. - So if our hypothesis is correct then the reaction rate of the mutant closer to the active site (I553W) would be ...
... - We will measure the rate of reaction of each enzyme. - If the reaction rate is slower for a mutant, then it shows that there is a major effect if we change that particular amino acid. - So if our hypothesis is correct then the reaction rate of the mutant closer to the active site (I553W) would be ...
Exploring the Human Genome - Cayetano Heredia University
... • For all mouse genes on chromosome 10 that are protein coding, I would like to know the IDs in both Ensembl and MGI. • In the query: Attributes: columns in the Result Table Filters: what we know ...
... • For all mouse genes on chromosome 10 that are protein coding, I would like to know the IDs in both Ensembl and MGI. • In the query: Attributes: columns in the Result Table Filters: what we know ...
Comparison of Protein Active Site Structures for
... tion of consensus templates for each functional family. Similarly, CPASS does not attempt to simulate generic features of active sites by using descriptors mimicking important properties of amino acids. Instead, the CPASS database is composed of ligand-defined protein active site structures culled f ...
... tion of consensus templates for each functional family. Similarly, CPASS does not attempt to simulate generic features of active sites by using descriptors mimicking important properties of amino acids. Instead, the CPASS database is composed of ligand-defined protein active site structures culled f ...
How to accelerate protein search on DNA: Location and dissociation
... of protein molecules to find and recognize quickly specific target sites on DNA. Although these phenomena have been studied extensively, detailed mechanisms of protein-DNA interactions during the search are still not well understood. Experiments suggest that proteins typically find their targets fas ...
... of protein molecules to find and recognize quickly specific target sites on DNA. Although these phenomena have been studied extensively, detailed mechanisms of protein-DNA interactions during the search are still not well understood. Experiments suggest that proteins typically find their targets fas ...
Introduction to Basic, Hypothalamic, and Hypophysial Endocrinology
... A molecule that functions as a message within an organism; its only function is to convey information. Because of this function, physical descriptions of a chemical suspected of being a hormone are not adequate to indicate the molecule's physiological role. A molecule is a hormone only when describe ...
... A molecule that functions as a message within an organism; its only function is to convey information. Because of this function, physical descriptions of a chemical suspected of being a hormone are not adequate to indicate the molecule's physiological role. A molecule is a hormone only when describe ...
MASCOT®
... on any of the platforms mentioned in this brochure. The one-time licence fee is based only on the number of processors used for searching. On a multiprocessor server or cluster, this can be less than the total number of processors available. The licence does not limit the number of users or restrict ...
... on any of the platforms mentioned in this brochure. The one-time licence fee is based only on the number of processors used for searching. On a multiprocessor server or cluster, this can be less than the total number of processors available. The licence does not limit the number of users or restrict ...
University of Groningen Archaeal type IV prepilin-like signal
... even before the Archaea were recognized as a distinct domain of life by Woese and Fox in 1977 (237) it was evident that the structure of their cell envelope differs substantially from that of bacteria. The cell envelope of archaea must withstand extreme conditions to ensure that essential membrane-r ...
... even before the Archaea were recognized as a distinct domain of life by Woese and Fox in 1977 (237) it was evident that the structure of their cell envelope differs substantially from that of bacteria. The cell envelope of archaea must withstand extreme conditions to ensure that essential membrane-r ...
Computer-based analyses of the protein constituents
... conducted for the OMA family where a sliding window of 7 residues was used. Similarly, a sliding window of 7 residues for the OMA family and of 20 residues for all other families was used to generate the mean similarity plots. Most of these analyses are not presented and are merely described in the ...
... conducted for the OMA family where a sliding window of 7 residues was used. Similarly, a sliding window of 7 residues for the OMA family and of 20 residues for all other families was used to generate the mean similarity plots. Most of these analyses are not presented and are merely described in the ...
Bioinformatics - Indiana University
... • The Ub SNCA ultimately forms the degraded Alphasynuclein through the Proteosome pathway. • If Alpha-synuclein degradation is blocked or defective, it aggregates to form the Lewy Body, which ultimately results in the dopaminergic cell death ...
... • The Ub SNCA ultimately forms the degraded Alphasynuclein through the Proteosome pathway. • If Alpha-synuclein degradation is blocked or defective, it aggregates to form the Lewy Body, which ultimately results in the dopaminergic cell death ...
The TCP domain: a motif found in proteins regulating plant growth
... indicates that the conserved domain most probably defines a new family of transcription factors, which we have termed the TCP family after its first characterised members (TB1, CYC and PCFs). Other plant proteins of unknown function also belong to this family. We have studied two of these in Arabido ...
... indicates that the conserved domain most probably defines a new family of transcription factors, which we have termed the TCP family after its first characterised members (TB1, CYC and PCFs). Other plant proteins of unknown function also belong to this family. We have studied two of these in Arabido ...
Biological Polymers - McQuarrie General Chemistry
... T-3. The Shape of a Protein Molecule Is Called Its Tertiary Structure A key step in understanding how a particular protein functions is the determination of its shape. Because many proteins are extremely large molecules, this task is not easy. The definitive method for determining a protein’s struct ...
... T-3. The Shape of a Protein Molecule Is Called Its Tertiary Structure A key step in understanding how a particular protein functions is the determination of its shape. Because many proteins are extremely large molecules, this task is not easy. The definitive method for determining a protein’s struct ...
Machine learning projects
... Proteins are sequences of small molecules (amino-acids) which arrange in a certain three dimensional structure (fold) Knowing the 3D structure of a protein is much harder than knowing their 1D structure (the sequence of amino-acids, represented as a string of letters, one for each amino-acid) It wou ...
... Proteins are sequences of small molecules (amino-acids) which arrange in a certain three dimensional structure (fold) Knowing the 3D structure of a protein is much harder than knowing their 1D structure (the sequence of amino-acids, represented as a string of letters, one for each amino-acid) It wou ...
Ch 7 Slides - people.iup.edu
... Many proteins can move or drift laterally as well but they do not flip-flop ...
... Many proteins can move or drift laterally as well but they do not flip-flop ...
pMAL FAQs
... For fusions expressed in the cytoplasm, in many cases most of the degradation happens during harvest and lysis. Harvesting promptly and lysing the cells quickly may help. In other cases, degradation occurs when the fusion protein is exposed to periplasmic or outer membrane proteases. The best strate ...
... For fusions expressed in the cytoplasm, in many cases most of the degradation happens during harvest and lysis. Harvesting promptly and lysing the cells quickly may help. In other cases, degradation occurs when the fusion protein is exposed to periplasmic or outer membrane proteases. The best strate ...
Ion Exchange Chromatography
... molecules based on ionic interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided into: cation exchange chromatography anion exchange chromatography. Dr Gihan Gawish ...
... molecules based on ionic interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided into: cation exchange chromatography anion exchange chromatography. Dr Gihan Gawish ...
Microcalorimetry of biological macromolecules
... being heavily S–S crosslinked does not unfolds completely even at 120 °C. For all other proteins, the averaged asymptotic limit at 120 °C for the enthalpy of unfolding is about 7 ± 1 kJ/ (mol-res) and for the entropy about 20 ± 2 J/K(mol-res). These values of the total enthalpies and entropies of un ...
... being heavily S–S crosslinked does not unfolds completely even at 120 °C. For all other proteins, the averaged asymptotic limit at 120 °C for the enthalpy of unfolding is about 7 ± 1 kJ/ (mol-res) and for the entropy about 20 ± 2 J/K(mol-res). These values of the total enthalpies and entropies of un ...
- 1 - 1. Which statement about mitochondria is CORRECT? a
... b) The minor groove presents four different configurations of hydrogen-bond donor, hydrogen-bond acceptor, hydrogen atom and methyl group; whereas, the major groove presents two. c) Some gene regulatory proteins have DNA-binding motifs that are spaced by 3.4nm, as this places the motifs on the same ...
... b) The minor groove presents four different configurations of hydrogen-bond donor, hydrogen-bond acceptor, hydrogen atom and methyl group; whereas, the major groove presents two. c) Some gene regulatory proteins have DNA-binding motifs that are spaced by 3.4nm, as this places the motifs on the same ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.