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Targeted transposition of the Sleeping Beauty - diss.fu
... in these genetic diseases is haemapoetic stem cell transplantation from a human leucocyte antigen-matched donor. If no compatible donor is found gene therapy can be considered. In inherited diseases introducing a functional copy of the defective gene will improve the disease phenotype or even fully ...
... in these genetic diseases is haemapoetic stem cell transplantation from a human leucocyte antigen-matched donor. If no compatible donor is found gene therapy can be considered. In inherited diseases introducing a functional copy of the defective gene will improve the disease phenotype or even fully ...
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... (Dequeant and Pourquie, 2008). Furthermore, these three pathways interact reciprocally within the mechanism of the mouse segmentation clock (Dequeant and Pourquie, 2008; Gibb et al., 2010; Maroto et al., 2012; Niwa et al., 2007). It is widely accepted, on a single cell level in the vertebrate PSM, t ...
... (Dequeant and Pourquie, 2008). Furthermore, these three pathways interact reciprocally within the mechanism of the mouse segmentation clock (Dequeant and Pourquie, 2008; Gibb et al., 2010; Maroto et al., 2012; Niwa et al., 2007). It is widely accepted, on a single cell level in the vertebrate PSM, t ...
Two-Component Systems of Mycobacterium tuberculosis as
... and dosS are conserved and tandemly arranged in many mycobacterial species (except M. leprae and M. ulcerans), while dosT appears to be less well conserved. DosS and DosT are capable of autophosphorylating at conserved histidine residues (His-395 and His-392, respectively), and both proteins can tra ...
... and dosS are conserved and tandemly arranged in many mycobacterial species (except M. leprae and M. ulcerans), while dosT appears to be less well conserved. DosS and DosT are capable of autophosphorylating at conserved histidine residues (His-395 and His-392, respectively), and both proteins can tra ...
Seed Germination of GA-Insensitive sleepy1
... using SLY1 gene-specific primers (Figure 1B). When the SLY1specific primers were employed, no significant change in mRNA accumulation was observed in sly1-10 and sly1-2, whereas a marked decrease was seen in sly1-t2. These results indicate that SLY1 transcript accumulation is reduced by the T-DNA in ...
... using SLY1 gene-specific primers (Figure 1B). When the SLY1specific primers were employed, no significant change in mRNA accumulation was observed in sly1-10 and sly1-2, whereas a marked decrease was seen in sly1-t2. These results indicate that SLY1 transcript accumulation is reduced by the T-DNA in ...
original version
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
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... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
The origin of a derived superkingdom: how a gram
... a major driver in the origin of archaea, but we feel our scenario better sets the stage for why this was a unique event. Antibiotic warfare on its own is not enough to account for the vast differences between the prokaryotic superkingdoms, but it certainly was important. James Lake and colleagues ha ...
... a major driver in the origin of archaea, but we feel our scenario better sets the stage for why this was a unique event. Antibiotic warfare on its own is not enough to account for the vast differences between the prokaryotic superkingdoms, but it certainly was important. James Lake and colleagues ha ...
Universitat Autònoma de Barcelona
... 2. Aggregation of the neuroblastoma-associated mutant (S120G) of the human nucleoside diphosphate kinase-A/NM23-H1 into amyloid fibrils Georgescauld F, Sabaté R, Espargaró A, Ventura S, Chaignepain S, Lacombe ML, Lascu I Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Ba ...
... 2. Aggregation of the neuroblastoma-associated mutant (S120G) of the human nucleoside diphosphate kinase-A/NM23-H1 into amyloid fibrils Georgescauld F, Sabaté R, Espargaró A, Ventura S, Chaignepain S, Lacombe ML, Lascu I Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Ba ...
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... mutations of PP1 in fungi could be (partially) complemented by expression of mammalian PP1 (113, 311), indicating that PP1 is also functionally conserved. Eukaryotic genomes contain one (Saccharomyces cerevisiae) to eight genes (Arabidopsis thaliana) encoding PP1 isoforms. More than 70% of the resid ...
... mutations of PP1 in fungi could be (partially) complemented by expression of mammalian PP1 (113, 311), indicating that PP1 is also functionally conserved. Eukaryotic genomes contain one (Saccharomyces cerevisiae) to eight genes (Arabidopsis thaliana) encoding PP1 isoforms. More than 70% of the resid ...
A single gene encodes two differentially targeted
... extensively studied in Arabidopsis thaliana (Figure 1). All the MEP pathway enzymes ...
... extensively studied in Arabidopsis thaliana (Figure 1). All the MEP pathway enzymes ...
HOLINS: The Protein Clocks of Bacteriophage Infections
... structurally other than that all three are monomeric, globular, and, at ∼18 kDa, relatively small (23, 30, 92). Recently, mosaicism within endolysin genes has been documented in a Staphylococcus phage, where different murein-binding domains and two muralytic activities were found in endolysins rangi ...
... structurally other than that all three are monomeric, globular, and, at ∼18 kDa, relatively small (23, 30, 92). Recently, mosaicism within endolysin genes has been documented in a Staphylococcus phage, where different murein-binding domains and two muralytic activities were found in endolysins rangi ...
Selective kinase inhibitors as tools for neuroscience research
... a known substrate of the target kinase, as use of the compound at concentrations significantly higher than this is much more likely to result in an off target activity. It is important to consider however that more than one kinase can act upon the same substrate site at once e for instance the transc ...
... a known substrate of the target kinase, as use of the compound at concentrations significantly higher than this is much more likely to result in an off target activity. It is important to consider however that more than one kinase can act upon the same substrate site at once e for instance the transc ...
Combining Rational and Evolutionary Approaches
... synthetic RNA switch to screen for improvements in enzymatic activity in vivo, I increased the activity of a model enzyme more than 30-fold. I then used a systems-level analysis of the host to identify a stress, heme depletion, that the enzyme placed on its host. Alleviating that stress increased th ...
... synthetic RNA switch to screen for improvements in enzymatic activity in vivo, I increased the activity of a model enzyme more than 30-fold. I then used a systems-level analysis of the host to identify a stress, heme depletion, that the enzyme placed on its host. Alleviating that stress increased th ...
ABSTRACT Title of Document:
... development, including senescence, abcission, fruit ripening, and responses to biotic and abiotic stresses. To find new members of the ethylene signaling pathway, a genetic screen for suppressors of the ethylene-insensitive mutant etr1-2 was performed. One mutant identified in this screen, etr1-11, ...
... development, including senescence, abcission, fruit ripening, and responses to biotic and abiotic stresses. To find new members of the ethylene signaling pathway, a genetic screen for suppressors of the ethylene-insensitive mutant etr1-2 was performed. One mutant identified in this screen, etr1-11, ...
The muscle fiber type–fiber size paradox: hypertrophy or oxidative
... synthesis of a limited amount of cytoplasm. In mammalian as well as amphibian muscle fibers, the number of myonuclei is positively related to the cross-sectional area of the fibers (Allen et al. 1995; Jaspers et al. 2006). Comparison of myonuclear numbers in high and low oxidative fibers has shown t ...
... synthesis of a limited amount of cytoplasm. In mammalian as well as amphibian muscle fibers, the number of myonuclei is positively related to the cross-sectional area of the fibers (Allen et al. 1995; Jaspers et al. 2006). Comparison of myonuclear numbers in high and low oxidative fibers has shown t ...
Novel Insights into Rice Innate Immunity Against Bacterial and
... pv. oryzae strains (135). A sulfated peptide called axYS 22, which was derived from the X. oryzae– secreted protein Ax21, was proposed to trigger Xa21-mediated resistance by binding to the LRR domain of the XA21 protein (76). Because this peptide is present in most Xanthomonas species, Ax21 was cons ...
... pv. oryzae strains (135). A sulfated peptide called axYS 22, which was derived from the X. oryzae– secreted protein Ax21, was proposed to trigger Xa21-mediated resistance by binding to the LRR domain of the XA21 protein (76). Because this peptide is present in most Xanthomonas species, Ax21 was cons ...
Presequence-Independent Manner Transported by the TIM23
... the carrier translocase (TIM22 complex), for their import. MPV17 and its Saccharomyces cerevisiae orthologue Sym1 are multispanning inner membrane proteins of unknown function with an amino-terminal presequence that suggests they may be targeted to the mitochondria. Mutations affecting MPV17 are ass ...
... the carrier translocase (TIM22 complex), for their import. MPV17 and its Saccharomyces cerevisiae orthologue Sym1 are multispanning inner membrane proteins of unknown function with an amino-terminal presequence that suggests they may be targeted to the mitochondria. Mutations affecting MPV17 are ass ...
Gene Section ALDOB (aldolase B, fructose-bisphosphate) Atlas of Genetics and Cytogenetics
... Many types of mutation in human ALDOB gene were reported, including missense mutations, nonsense mutations, deletions, insertions and mutation at the splicing regions (list in the diagram above). The mutations bring about reduced enzyme activity and affect structural stability. Mutants that retained ...
... Many types of mutation in human ALDOB gene were reported, including missense mutations, nonsense mutations, deletions, insertions and mutation at the splicing regions (list in the diagram above). The mutations bring about reduced enzyme activity and affect structural stability. Mutants that retained ...
Frequently Asked Questions (FAQs) What is this application related
... The assessment for the effect of this GE mustard on microbial population has revealed no adverse effect on soil microorganisms. Also, the three introduced proteins have been found to be easily digested by the simulated gastric fluid. Therefore, based on the history of safe use of these proteins in o ...
... The assessment for the effect of this GE mustard on microbial population has revealed no adverse effect on soil microorganisms. Also, the three introduced proteins have been found to be easily digested by the simulated gastric fluid. Therefore, based on the history of safe use of these proteins in o ...
Natalia Gromak, Alexis Rideau,
... mutations at each of the three PTB consensus sites, while mutations of the DY pyrimidine tract involved complete deletion (DDY) or deletion of 12 nt including the two overlapping optimal PTB sites (DYDPC). As expected, compared with the wild-type construct all of the mutants had decreased amounts of ...
... mutations at each of the three PTB consensus sites, while mutations of the DY pyrimidine tract involved complete deletion (DDY) or deletion of 12 nt including the two overlapping optimal PTB sites (DYDPC). As expected, compared with the wild-type construct all of the mutants had decreased amounts of ...
Identification and analysis of new phloem proteins from
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
ftsZ mutations affecting cell division frequency, placement and
... from the minD mutant, and one (ftsZ8) from the minCD mutant. All of the mutants appeared to be further impaired in sporulation than the parental strain on NA. The mutations were crossed into the minCD+ strain 1272. Segregation of mutations ftsZ5, ftsZ20 and ftsZ38 into colonies with normal and reduc ...
... from the minD mutant, and one (ftsZ8) from the minCD mutant. All of the mutants appeared to be further impaired in sporulation than the parental strain on NA. The mutations were crossed into the minCD+ strain 1272. Segregation of mutations ftsZ5, ftsZ20 and ftsZ38 into colonies with normal and reduc ...
Accommodating the bacterial decoding release factor as an
... factor communicated directly or indirectly with the two active sites on the ribosome for decoding and catalysis. A direct decoding model would imply the decoding RF might have a structural feature similar to the tRNA anticodon to recognize the stop codon directly and, as described below, this appear ...
... factor communicated directly or indirectly with the two active sites on the ribosome for decoding and catalysis. A direct decoding model would imply the decoding RF might have a structural feature similar to the tRNA anticodon to recognize the stop codon directly and, as described below, this appear ...
Raw Glycerol as Substrate for the Production of Yeast Biomass
... Y. lipolytica NRRL YB-423, C. lipolytica NRRL Y-1095, C. utilis NRRL Y-900 and C. rugosa NRRL Y-95 were performed using raw glycerol as the main carbon source. They were then compared with the results obtained when analytical grade glycerol was used as a substrate. Figure 1 shows microbial growth an ...
... Y. lipolytica NRRL YB-423, C. lipolytica NRRL Y-1095, C. utilis NRRL Y-900 and C. rugosa NRRL Y-95 were performed using raw glycerol as the main carbon source. They were then compared with the results obtained when analytical grade glycerol was used as a substrate. Figure 1 shows microbial growth an ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
... analysis of the markers mapped in more than one Rosaceae species revealed patterns of macro- and micro-synteny among Malus and Prunus (Dirlewanger et al. 2004; Chen et al. 2008; Sargent et al. 2009), and, because of this, genomic advances in peach are also of use in apple genetics (Shulaev et al. 20 ...
... analysis of the markers mapped in more than one Rosaceae species revealed patterns of macro- and micro-synteny among Malus and Prunus (Dirlewanger et al. 2004; Chen et al. 2008; Sargent et al. 2009), and, because of this, genomic advances in peach are also of use in apple genetics (Shulaev et al. 20 ...
Protein moonlighting
![](https://commons.wikimedia.org/wiki/Special:FilePath/3EL3.png?width=300)
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.