from dicp.ac.cn
... activate the holoenzyme (17). p38 and CK2 both co-immunoprecipitate with p53 (18, 19). Anisomycin and tumor necrosis factor-α (TNF-α)-induced phosphorylation of p53 at Ser392 requires p38 MAPK kinase and CK2 activities (17). In additional, CK2 can phosphorylate IκBα at a cluster of C-terminal sites ...
... activate the holoenzyme (17). p38 and CK2 both co-immunoprecipitate with p53 (18, 19). Anisomycin and tumor necrosis factor-α (TNF-α)-induced phosphorylation of p53 at Ser392 requires p38 MAPK kinase and CK2 activities (17). In additional, CK2 can phosphorylate IκBα at a cluster of C-terminal sites ...
elucidate the contribution of proteins to tears. a challenge for
... Beneath the dominant, and almost dismissive idea that the roles for proteins in the tears are antimicrobial and immunological, has been a persistent undercurrent from stalwarts investigating the physical contribution of proteins to tears. It is here that we enter the realms of surface tension and vi ...
... Beneath the dominant, and almost dismissive idea that the roles for proteins in the tears are antimicrobial and immunological, has been a persistent undercurrent from stalwarts investigating the physical contribution of proteins to tears. It is here that we enter the realms of surface tension and vi ...
Bio 103 Lecture - Origin and Evolution of Micr
... and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what was the temperature around the time life appeared, what caused the change in temperature? Prokaryotes have inhabited the earth for billions of years • when w ...
... and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what was the temperature around the time life appeared, what caused the change in temperature? Prokaryotes have inhabited the earth for billions of years • when w ...
Structural determinants for rCNT2 sorting to the plasma membrane
... (see Supplementary Table S1 at http://www.BiochemJ.org/ bj/442/bj4420517add.htm) showed similar expression levels for all these proteins, so uptake changes are due to differential activity and/or localization. Guanosine-uptake assays showed significantly impaired activity when the N-terminal region ...
... (see Supplementary Table S1 at http://www.BiochemJ.org/ bj/442/bj4420517add.htm) showed similar expression levels for all these proteins, so uptake changes are due to differential activity and/or localization. Guanosine-uptake assays showed significantly impaired activity when the N-terminal region ...
From Flower to Seed Germination in Cynara cardunculus: A Role for
... Cardosins are plant aspartic proteinases (APs) isolated from the flowers of Cynara cardunculus L. (cardoon) and are responsible for their milk-clotting activity used to manufacture ewe’s cheese. Cardosin A is mainly accumulated in protein storage vacuoles of the stigmatic papillae being suggested a ...
... Cardosins are plant aspartic proteinases (APs) isolated from the flowers of Cynara cardunculus L. (cardoon) and are responsible for their milk-clotting activity used to manufacture ewe’s cheese. Cardosin A is mainly accumulated in protein storage vacuoles of the stigmatic papillae being suggested a ...
CHAP NUM="5" ID="CH
... most common monosaccharide, is of central importance in the chemistry of life. In the structure of glucose, we can see the trademarks of a sugar: The molecule has a carbonyl group ([see C8e page 70 for correct slash style] C O ) and multiple hydroxyl groups (OH). Depending on the location of the ...
... most common monosaccharide, is of central importance in the chemistry of life. In the structure of glucose, we can see the trademarks of a sugar: The molecule has a carbonyl group ([see C8e page 70 for correct slash style] C O ) and multiple hydroxyl groups (OH). Depending on the location of the ...
A Lipid Droplet Protein of Nannochloropsis with
... increase in LD size compared to wild-type plants (Siloto et al., 2006; Fig. 3, A–D). This phenotype was mostly reversed in the presence of LDSP-His, as shown in Figure 3, E to H, even though the two lines did not show LD size reduction to the same extent. Whereas line R5-5 produced embryos with no o ...
... increase in LD size compared to wild-type plants (Siloto et al., 2006; Fig. 3, A–D). This phenotype was mostly reversed in the presence of LDSP-His, as shown in Figure 3, E to H, even though the two lines did not show LD size reduction to the same extent. Whereas line R5-5 produced embryos with no o ...
Exploration of the Dynamic Properties of Protein Complexes
... introduce new components and deplete existing components through exchange. While it is clear that the structure and function of any given protein complex is coupled to its dynamical properties, it remains a challenge to predict the possible conformations that complexes can adopt. Protein-fragment Co ...
... introduce new components and deplete existing components through exchange. While it is clear that the structure and function of any given protein complex is coupled to its dynamical properties, it remains a challenge to predict the possible conformations that complexes can adopt. Protein-fragment Co ...
Table S1.
... Rv3742c Function unknown; probably involved in cellular metabolism. Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by ...
... Rv3742c Function unknown; probably involved in cellular metabolism. Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by ...
Biochemistry
... (h) Define the Beer Lambert Law. The A340 of a solution of NADH was found to be 0.29. What was the concentration of this solution? (εNADH = 6220 M-1 cm-1). (i) Although oxygen does not participate directly in the citric acid cycle, the cycle operates only when O2 is present. Why? (j) Calculate the n ...
... (h) Define the Beer Lambert Law. The A340 of a solution of NADH was found to be 0.29. What was the concentration of this solution? (εNADH = 6220 M-1 cm-1). (i) Although oxygen does not participate directly in the citric acid cycle, the cycle operates only when O2 is present. Why? (j) Calculate the n ...
1 (a)
... authors made use of three commercial immunoaffinity depletion methods, the multiple affinity removal system (MARS), prior to proteomic analysis. 4. Immunodepleted serum: The serum from which the high abundance proteins have been removed, leaving behind only the medium and low abundance proteins ther ...
... authors made use of three commercial immunoaffinity depletion methods, the multiple affinity removal system (MARS), prior to proteomic analysis. 4. Immunodepleted serum: The serum from which the high abundance proteins have been removed, leaving behind only the medium and low abundance proteins ther ...
ERdj5, an Endoplasmic Reticulum (ER)
... with the features of DnaJ/Hsp40 proteins, including the HisPro-Asp motif, is encountered (Fig. 2A). Neither G/F-rich nor cysteine-rich regions are present, thus making ERdj5 a type III DnaJ protein (14, 29). Following the DnaJ domain are four thioredoxin-like domains, with different putative redox a ...
... with the features of DnaJ/Hsp40 proteins, including the HisPro-Asp motif, is encountered (Fig. 2A). Neither G/F-rich nor cysteine-rich regions are present, thus making ERdj5 a type III DnaJ protein (14, 29). Following the DnaJ domain are four thioredoxin-like domains, with different putative redox a ...
1998 warkany lecture: Signaling pathways in development
... As information transfer pathways, these signaling pathways are basically different from metabolic pathways, even though both are called ‘‘pathways.’’ In a biosynthetic metabolic pathway, a carbon compound passes through a series of enzymatic steps, with appropriate energy inputs, undergoing modifica ...
... As information transfer pathways, these signaling pathways are basically different from metabolic pathways, even though both are called ‘‘pathways.’’ In a biosynthetic metabolic pathway, a carbon compound passes through a series of enzymatic steps, with appropriate energy inputs, undergoing modifica ...
REVIEWS
... structural fluctuations and not merely on the timeaveraged structure. Even when completely folded, both wild-type and mutant proteins might unfold transiently and expose chaperone-binding, misfolded conformations when they are in the ER. The lower the overall stability of a protein, the more frequen ...
... structural fluctuations and not merely on the timeaveraged structure. Even when completely folded, both wild-type and mutant proteins might unfold transiently and expose chaperone-binding, misfolded conformations when they are in the ER. The lower the overall stability of a protein, the more frequen ...
TRANSMEMBRANE ADAPTOR PROTEINS: ORGANIZERS OF
... a variable number of tyrosine residues within tyrosinebased signalling motifs. However, none of the known TRAPs have classical ITAMs, and in general, TRAPs do not associate directly with immunoreceptors; an exception might be the TCR-interacting molecule (TRIM) (discussed later). Nevertheless, the g ...
... a variable number of tyrosine residues within tyrosinebased signalling motifs. However, none of the known TRAPs have classical ITAMs, and in general, TRAPs do not associate directly with immunoreceptors; an exception might be the TCR-interacting molecule (TRIM) (discussed later). Nevertheless, the g ...
Supplementary feeding of grazing sheep in South Africa
... of lambs by maximizing their growth rate and improving their feed conversion ratio by the supplementation of a Pasture Finisher supplement (± 1.5 % of body weight) with a very high level of bypass protein. With a high bypass protein intake, most of the weight gain or the growth of the lamb is in the ...
... of lambs by maximizing their growth rate and improving their feed conversion ratio by the supplementation of a Pasture Finisher supplement (± 1.5 % of body weight) with a very high level of bypass protein. With a high bypass protein intake, most of the weight gain or the growth of the lamb is in the ...
Designed Ankyrin Repeat Proteins (DARPins)
... more ambitious formats (as fusions to other aggregation-prone proteins, in multimers, or in the absence of disulfide bonds), the limitations in their biophysical properties become even more apparent. ...
... more ambitious formats (as fusions to other aggregation-prone proteins, in multimers, or in the absence of disulfide bonds), the limitations in their biophysical properties become even more apparent. ...
Nitric Oxide Signalling in Plants: Cross
... Currently, NO is recognized as one of the key messengers governing the overall control of Ca2+ homeostasis, and almost all types of Ca2+ channels and transporters are under its control. The effects of NO on Ca2+ channel and transporter activities can be divided into two mechanisms of action: a cGMP ...
... Currently, NO is recognized as one of the key messengers governing the overall control of Ca2+ homeostasis, and almost all types of Ca2+ channels and transporters are under its control. The effects of NO on Ca2+ channel and transporter activities can be divided into two mechanisms of action: a cGMP ...
MAPK Review
... Scaffolding proteins Scaffolds have a major influence on cascade function strongly impacting on the activities and outputs of MAPK pathways. Best described by the yeast example Ste5, scaffolds are paramount for achieving MAPK specificity.64,70,71 The yeast MAP3K, Ste11, can activate either Ste7, a M ...
... Scaffolding proteins Scaffolds have a major influence on cascade function strongly impacting on the activities and outputs of MAPK pathways. Best described by the yeast example Ste5, scaffolds are paramount for achieving MAPK specificity.64,70,71 The yeast MAP3K, Ste11, can activate either Ste7, a M ...
binding domains demonstrated in a plant split
... purified as soluble recombinant proteins from bacteria, they exist in a concentration-dependent equilibrium between the monomeric and dimeric state (Reddick et al., 2007; Yeh et al., 2007). This observation and the crystal structures available for Arabidopsis and pea Toc33 indicate the formation of ...
... purified as soluble recombinant proteins from bacteria, they exist in a concentration-dependent equilibrium between the monomeric and dimeric state (Reddick et al., 2007; Yeh et al., 2007). This observation and the crystal structures available for Arabidopsis and pea Toc33 indicate the formation of ...
Hey Adrian !!! Pass the Egg Whites
... Although this short "Anabolic Window" is a critical period of time, it's during the next 24 to 48 hours that most muscle growth occurs. The downside of all protein powders is that they only provide anabolic support for a couple of hours which is far less than what your body really needs... This is w ...
... Although this short "Anabolic Window" is a critical period of time, it's during the next 24 to 48 hours that most muscle growth occurs. The downside of all protein powders is that they only provide anabolic support for a couple of hours which is far less than what your body really needs... This is w ...
Rhizobiales-like protein phosphatases (Rhilphs): A role in plant
... superfamily, which are structurally more similar to bacterial phosphatases than to canonical phosphatases from eukaryotes (Andreeva and Kutuzov, 1999; Andreeva and Kutuzov, 2004). One of the groups of these “bacterial-like” phosphatases is most closely related to PPP phosphatases from some α -Proteo ...
... superfamily, which are structurally more similar to bacterial phosphatases than to canonical phosphatases from eukaryotes (Andreeva and Kutuzov, 1999; Andreeva and Kutuzov, 2004). One of the groups of these “bacterial-like” phosphatases is most closely related to PPP phosphatases from some α -Proteo ...
Proteins and Enzymes
... Evolution of proteins • Some pre-RNA polymer is believed to have come first; RNA evolved from that • RNA can function as enzymes • RNA became the template for DNA • Amino acids are attracted to codons • RNA catalyzed condensation of amino acids • Ribosomes evolved to increase efficiency and ...
... Evolution of proteins • Some pre-RNA polymer is believed to have come first; RNA evolved from that • RNA can function as enzymes • RNA became the template for DNA • Amino acids are attracted to codons • RNA catalyzed condensation of amino acids • Ribosomes evolved to increase efficiency and ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.