Expression of Hepatitis C Virus Structural Proteins in
... 250 kDa protein was reacted with anti-E2, anti-E1, and anti-core antibodies (Figs. 3A, 3B and 3C, respectively). This large protein was assumed to be a nonprocessed aggregated form. The E1 protein was also detected as a slightly smeared form of 35 kDa in size by Western blot (Fig 3B). When we used m ...
... 250 kDa protein was reacted with anti-E2, anti-E1, and anti-core antibodies (Figs. 3A, 3B and 3C, respectively). This large protein was assumed to be a nonprocessed aggregated form. The E1 protein was also detected as a slightly smeared form of 35 kDa in size by Western blot (Fig 3B). When we used m ...
Mutational analysis of the early f orespore/mother
... pDG1664 (Guerout-Fleury et al., 1996). In addition, the BspHI site overlapping the AM23 mutation allowed us to construct a version of spoZZR lacking the first 22 codons after several subcloning steps aimed at replacing the Shine-Dalgarno sequence in front of the methionine. This version was also int ...
... pDG1664 (Guerout-Fleury et al., 1996). In addition, the BspHI site overlapping the AM23 mutation allowed us to construct a version of spoZZR lacking the first 22 codons after several subcloning steps aimed at replacing the Shine-Dalgarno sequence in front of the methionine. This version was also int ...
Photosynthesis is the portal through which the energy of sunlight
... identified domains that are highly variable in amino acid composition. Since we can assume that these diverse psbS proteins are capable of causing quenching in their native species, the variable domains could include changes that were adaptive during the process of speciation. Unfortunately, testing ...
... identified domains that are highly variable in amino acid composition. Since we can assume that these diverse psbS proteins are capable of causing quenching in their native species, the variable domains could include changes that were adaptive during the process of speciation. Unfortunately, testing ...
further characterization of the f1
... cyclic 3',5'-adenosine monophosphate (cAMP)-dependent phosphokinase, differs from KII by several criteria. KII is mol wt 90,000, cAMP independent, rapidly turned over in vivo, low K. for ATP, and phosphorylates FI histone at several unique sites. Comparative examination of metaphase-arrested (M) and ...
... cyclic 3',5'-adenosine monophosphate (cAMP)-dependent phosphokinase, differs from KII by several criteria. KII is mol wt 90,000, cAMP independent, rapidly turned over in vivo, low K. for ATP, and phosphorylates FI histone at several unique sites. Comparative examination of metaphase-arrested (M) and ...
The origin of the eukaryotic cell: A genomic investigation
... proteins having diverged from a common ancestor or was this structural similarity caused by the convergence of these proteins from different ancestral proteins? There is at present no good methodology for distinguishing between these two alternatives. There are a number of ESP proteins, e.g., ubiqu ...
... proteins having diverged from a common ancestor or was this structural similarity caused by the convergence of these proteins from different ancestral proteins? There is at present no good methodology for distinguishing between these two alternatives. There are a number of ESP proteins, e.g., ubiqu ...
Recent advances in technology for measuring and manipulating cell
... David A Zacharias*, Geoffrey S Baird† and Roger Y Tsien‡ Signal transduction research has made some glowing progress in the past 12 months. Recent advances in fluorescent proteins, small molecule fluorophores and imaging technology are generating new ways to investigate signal transduction. ...
... David A Zacharias*, Geoffrey S Baird† and Roger Y Tsien‡ Signal transduction research has made some glowing progress in the past 12 months. Recent advances in fluorescent proteins, small molecule fluorophores and imaging technology are generating new ways to investigate signal transduction. ...
Thalassiosira pseudonana Phosphorus Stress Response. Sonya T. Dyhrman
... modeled gene set (Table S1; S2). Past transcriptional profiling in this diatom using a tiling array detected transcription of 4,653 (43%) of the predicted protein coding genes [18]. Tag-seq compares favorably with RNA-seq [27], and is of equivalent or better coverage than other tiling array, DGE, or ...
... modeled gene set (Table S1; S2). Past transcriptional profiling in this diatom using a tiling array detected transcription of 4,653 (43%) of the predicted protein coding genes [18]. Tag-seq compares favorably with RNA-seq [27], and is of equivalent or better coverage than other tiling array, DGE, or ...
Amyloid Deposits: Protection Against Toxic Protein
... proteins critical for cell homeostasis29. Indeed, inclusions formed by mutant Huntingtin protein have been shown to sequester glyceraldehydes-3-phosphate dehydrogenase, to impair transcription due to sequestration of the transcriptional coactivator CREB binding protein, and to interfere with the fun ...
... proteins critical for cell homeostasis29. Indeed, inclusions formed by mutant Huntingtin protein have been shown to sequester glyceraldehydes-3-phosphate dehydrogenase, to impair transcription due to sequestration of the transcriptional coactivator CREB binding protein, and to interfere with the fun ...
further characterization of the f1-histone
... separated from 0 .35 M NaCl extracts of whole randomly growing cells by G-200 Sephadex gelfiltration . As seen in Fig . 1 A, gel filtration at 0 .2 M NaCl is sufficient to prevent the aggregation of these two enzyme activities which apparently occurs at low salt . At very high salt, above 0 .5 M som ...
... separated from 0 .35 M NaCl extracts of whole randomly growing cells by G-200 Sephadex gelfiltration . As seen in Fig . 1 A, gel filtration at 0 .2 M NaCl is sufficient to prevent the aggregation of these two enzyme activities which apparently occurs at low salt . At very high salt, above 0 .5 M som ...
Fad Diets: Low Carbohydrate Diet Summaries
... 31 g/d sat fat (16%) 20 g/d fiber • Limited food choices • Diet low in fiber, vit D, thiamine, pantothenic acid, calcium, copper, vitamin C, magnesium, manganese, K+ • Too high in total and saturated fat and protein Yes. Initial weight loss is mostly water. Does not promote a positive attitude towar ...
... 31 g/d sat fat (16%) 20 g/d fiber • Limited food choices • Diet low in fiber, vit D, thiamine, pantothenic acid, calcium, copper, vitamin C, magnesium, manganese, K+ • Too high in total and saturated fat and protein Yes. Initial weight loss is mostly water. Does not promote a positive attitude towar ...
a review of the BLAST meeting, ï - Bacterial Locomotion and Signal
... liquids versus on surfaces. Likewise, a successful adaptive mechanism must sense the proper signals, which are as diverse as the environments occupied by bacteria and which range from small chemicals to specific wavelengths of light. In fact, what makes this field so exciting is the range and divers ...
... liquids versus on surfaces. Likewise, a successful adaptive mechanism must sense the proper signals, which are as diverse as the environments occupied by bacteria and which range from small chemicals to specific wavelengths of light. In fact, what makes this field so exciting is the range and divers ...
A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
Title Scaffold proteins in mammalian MAP kinase
... phosphatase (39), and OSM (osmosensing scaffold for MEKK3), a MEKK3-binding protein (40). Dynamic Regulation of MAPK Signaling Modules Mediated by Scaffold Proteins—MAPK scaffold proteins may function merely to increase the local concentration of their associated signaling components, as suggested b ...
... phosphatase (39), and OSM (osmosensing scaffold for MEKK3), a MEKK3-binding protein (40). Dynamic Regulation of MAPK Signaling Modules Mediated by Scaffold Proteins—MAPK scaffold proteins may function merely to increase the local concentration of their associated signaling components, as suggested b ...
Physiology and Cell Biology Research Day
... Brian Hansen, Q. Lou, L. Hage, O. Fedorenko, A. Kalyanasundaram, A.V. Glukhov, I.M. Bonilla, S. Györke, P.M.L. Janssen, D.R. Van Wagoner, P.J. Mohler, R. Weiss, B.J. Biesiadecki, C.A. Carnes, V.V. Fedorov 2. Apigenin enhances Akti1/2-induced Radioactive Iodine Uptake (RAIU) in Thyroid Cells in p38 M ...
... Brian Hansen, Q. Lou, L. Hage, O. Fedorenko, A. Kalyanasundaram, A.V. Glukhov, I.M. Bonilla, S. Györke, P.M.L. Janssen, D.R. Van Wagoner, P.J. Mohler, R. Weiss, B.J. Biesiadecki, C.A. Carnes, V.V. Fedorov 2. Apigenin enhances Akti1/2-induced Radioactive Iodine Uptake (RAIU) in Thyroid Cells in p38 M ...
Gene
... known to result in another resolution of the proteins (Overbeeke and Lugtenberg, 1980b). As shown in Fig. 18, an additional band was now found in the cell envelopes of the pEClO-containing strain (lane d), which migrates slightly faster in the gel than E. coli PhoE protein and which was not observed ...
... known to result in another resolution of the proteins (Overbeeke and Lugtenberg, 1980b). As shown in Fig. 18, an additional band was now found in the cell envelopes of the pEClO-containing strain (lane d), which migrates slightly faster in the gel than E. coli PhoE protein and which was not observed ...
Magic Numbers in Protein Structures
... thermodynamic theory [2]. Next we can evaluate how many distinct fold classes exist. If we restrict ourselves to domain structures with N # 17 we find in total 3906 possible, distinct globular fold classes. This is close to Chothia’s estimate of 1000 fold classes, based on a heuristic argument [14]. ...
... thermodynamic theory [2]. Next we can evaluate how many distinct fold classes exist. If we restrict ourselves to domain structures with N # 17 we find in total 3906 possible, distinct globular fold classes. This is close to Chothia’s estimate of 1000 fold classes, based on a heuristic argument [14]. ...
Cell A.
... Signal Transduction Scenerio 1.Activation of a tyrosine-kinase receptor causes phopholipase C to cleave PIP2 forming IP3 and DAG from the cell membrane. IP3 then binds to a ligand-gated ion channel on the ER causing the release of calcium ions, which bind to calmodulin & causes the cytoskeleton to ...
... Signal Transduction Scenerio 1.Activation of a tyrosine-kinase receptor causes phopholipase C to cleave PIP2 forming IP3 and DAG from the cell membrane. IP3 then binds to a ligand-gated ion channel on the ER causing the release of calcium ions, which bind to calmodulin & causes the cytoskeleton to ...
Integrin modulation of signaling to transcription factors
... Tournier et al., 1997). A variety of activators of MKK4 and MKK7 have been described, including MAP/ERK kinase kinases (MEKKs) 1-4, members of the mixed-lineage kinase family and the apoptosis-stimulated kinases (reviewed by Davis, 2000; Garrington and Johnson, 1999). Activated JNK translocates to t ...
... Tournier et al., 1997). A variety of activators of MKK4 and MKK7 have been described, including MAP/ERK kinase kinases (MEKKs) 1-4, members of the mixed-lineage kinase family and the apoptosis-stimulated kinases (reviewed by Davis, 2000; Garrington and Johnson, 1999). Activated JNK translocates to t ...
Induction of Sequence-Specific DNA
... a major role in the regulation of the growth and differentiation of hematopoietic cells in the erythroid lineage. The genes encoding Epo and the Epo receptor (EpoR) have been isolated, and the relationship between their structure and function has been studied extensively.”6However, the actual mechan ...
... a major role in the regulation of the growth and differentiation of hematopoietic cells in the erythroid lineage. The genes encoding Epo and the Epo receptor (EpoR) have been isolated, and the relationship between their structure and function has been studied extensively.”6However, the actual mechan ...
The intracellular cyanobacteria of Paulinella chromatophora
... from signal peptides [6,8]. In fact, structural and functional similarities between mitochondrial and plastid transit peptides, in addition to common interactions with cytosolic factors [9], indicate that the initial establishment of the mitochondrion could have pre-adapted eukaryotic cells for addi ...
... from signal peptides [6,8]. In fact, structural and functional similarities between mitochondrial and plastid transit peptides, in addition to common interactions with cytosolic factors [9], indicate that the initial establishment of the mitochondrion could have pre-adapted eukaryotic cells for addi ...
Protein translocation across mitochondrial membranes
... MOM38 is the equivalent of GIP(27).An independent approach led to the identification of this component in the import machinery in yeast. A translocation intermediate spanning contact sites was crosslinked using a bifunctional reagent to a 42 kDa component of the outer membrane(”). This yeast ISP42 ( ...
... MOM38 is the equivalent of GIP(27).An independent approach led to the identification of this component in the import machinery in yeast. A translocation intermediate spanning contact sites was crosslinked using a bifunctional reagent to a 42 kDa component of the outer membrane(”). This yeast ISP42 ( ...
Qproteome Nuclear Protein Handbook
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
BMC Microbiology
... the induction of the yeast to mycelium transition. The second calcium uptake peak was observed 300 min after induction, at the time of DNA synthesis. When different substances that affected calcium uptake were added to the medium during the yeast to mycelium transition such as cobalt ions, ionophore ...
... the induction of the yeast to mycelium transition. The second calcium uptake peak was observed 300 min after induction, at the time of DNA synthesis. When different substances that affected calcium uptake were added to the medium during the yeast to mycelium transition such as cobalt ions, ionophore ...
The Plant Journal
... cysteine-rich region. These domains are the site of DAG binding in protein kinase C (PKC) (Liu and Heckman, 1998), but it is not clear what function they perform in DGKs. Our work supports a recent ®nding (Sakane et al., 1996) suggesting that cysteine-rich regions are not necessary for DAG binding o ...
... cysteine-rich region. These domains are the site of DAG binding in protein kinase C (PKC) (Liu and Heckman, 1998), but it is not clear what function they perform in DGKs. Our work supports a recent ®nding (Sakane et al., 1996) suggesting that cysteine-rich regions are not necessary for DAG binding o ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.