"Lymphocyte Activation Signals: Transduction".
... stimulation to progress from the G0 stage of the cell cycle. The immune response to pathogens or foreign substances is initiated from clonotypic receptors on these cells that recognize the antigen and induce a series of signal transduction events. These signals are fundamental in both developmental ...
... stimulation to progress from the G0 stage of the cell cycle. The immune response to pathogens or foreign substances is initiated from clonotypic receptors on these cells that recognize the antigen and induce a series of signal transduction events. These signals are fundamental in both developmental ...
pMAL FAQs
... other strains in response to a particular problem. One can start with NEB Express, or with whatever competent cells are readily available, and then try another strain if a problem with expression or purification develops. 2. What primers should I use to sequence the ends of my insert after I clone i ...
... other strains in response to a particular problem. One can start with NEB Express, or with whatever competent cells are readily available, and then try another strain if a problem with expression or purification develops. 2. What primers should I use to sequence the ends of my insert after I clone i ...
Cell Cycle Regulation of the Activity and Subcellular Localization of
... spindle function and chromosome segregation is polo, a serine/threonine-specific enzyme first identified in Drosophila (Llamazares et al., 1991). Drosophila embryos harboring mutant polo alleles show a broad range of spindle abnormalities including monopolar spindles, highly branched bipolar spindle ...
... spindle function and chromosome segregation is polo, a serine/threonine-specific enzyme first identified in Drosophila (Llamazares et al., 1991). Drosophila embryos harboring mutant polo alleles show a broad range of spindle abnormalities including monopolar spindles, highly branched bipolar spindle ...
Document
... we developed (to screen out of the analysis most proteins that are highly conserved in all organisms, that BLAST may list as most similar to a protein from another Domain by chance). Various taxonomic levels of organisms are filtered from the BLAST results to aid identification of putative lateral t ...
... we developed (to screen out of the analysis most proteins that are highly conserved in all organisms, that BLAST may list as most similar to a protein from another Domain by chance). Various taxonomic levels of organisms are filtered from the BLAST results to aid identification of putative lateral t ...
TOTAL RECOVERY A Mind-Body Enhancer for Optimal Athletic
... reductions in free radical and CPK damage will translate into improved performance and better overall health for every athlete. In another study measuring anabolic muscle growth, the athletes were given either a protein and carbohydrate supplement immediately after exercise or three hours after a tr ...
... reductions in free radical and CPK damage will translate into improved performance and better overall health for every athlete. In another study measuring anabolic muscle growth, the athletes were given either a protein and carbohydrate supplement immediately after exercise or three hours after a tr ...
Lecture 15 Membrane Proteins I
... What are membrane proteins and where do they exist? Proteins consist of three main classes which are classified as globular, fibrous and membrane proteins. A cell is enveloped by a membrane which makes the boundary of a cell and enables it to maintain the distinction between cytosolic and extracellu ...
... What are membrane proteins and where do they exist? Proteins consist of three main classes which are classified as globular, fibrous and membrane proteins. A cell is enveloped by a membrane which makes the boundary of a cell and enables it to maintain the distinction between cytosolic and extracellu ...
Finding a way to the nucleus - Purdue University
... covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-complex, such a complex has not been demonstrated in plants. Interaction of VirD2/T-strands and VirE2 with other secr ...
... covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-complex, such a complex has not been demonstrated in plants. Interaction of VirD2/T-strands and VirE2 with other secr ...
The Internal Repeats in the Na /Ca2+ Exchanger
... not clear whether the two conserved ␣-motifs are located on the same or opposite sides of the membrane. Obviously, a resolution of this point will have important functional implications for this class of proteins. In general, both prediction and experimental determination of membrane protein topolog ...
... not clear whether the two conserved ␣-motifs are located on the same or opposite sides of the membrane. Obviously, a resolution of this point will have important functional implications for this class of proteins. In general, both prediction and experimental determination of membrane protein topolog ...
Chemotaxis
... (2) Bray et al. (1998) – sensitivity and range may result in part from clustering of receptors on surface of bacterium • postulate that change in receptor induced by ligand binding is propagated to neighboring receptors • postulate: (I) cell has both clusters and single receptors in equilibrium and ...
... (2) Bray et al. (1998) – sensitivity and range may result in part from clustering of receptors on surface of bacterium • postulate that change in receptor induced by ligand binding is propagated to neighboring receptors • postulate: (I) cell has both clusters and single receptors in equilibrium and ...
HIV-1 and Ebola virus encode small peptide motifs that recruit
... a)Tsg101 is a component of a complex,ESCRT-I that is essential for the sorting of ubiquitinated proteins into the multi-vesicular body (MVB) in yeast and for endosomal targeting in mammalian Cells. b)The ‘budding’ and membrane-fusion events that lead to the formation MVB are equivalent to the buddin ...
... a)Tsg101 is a component of a complex,ESCRT-I that is essential for the sorting of ubiquitinated proteins into the multi-vesicular body (MVB) in yeast and for endosomal targeting in mammalian Cells. b)The ‘budding’ and membrane-fusion events that lead to the formation MVB are equivalent to the buddin ...
Sequence-based prediction of protein interaction
... DNA sequencing methods. For example, by July 29, 2008, there are 392 667 identified protein sequences in Uniprot/Swissprot (reviewed, manually annotated) (Uniprot, 2008) and only 47 978 known protein structures in PDB (Berman et al., 2000). Thus, it is now more important than ever to identify protei ...
... DNA sequencing methods. For example, by July 29, 2008, there are 392 667 identified protein sequences in Uniprot/Swissprot (reviewed, manually annotated) (Uniprot, 2008) and only 47 978 known protein structures in PDB (Berman et al., 2000). Thus, it is now more important than ever to identify protei ...
Identification of NaHCO3 Stress Responsive Proteins in Dunaliella
... limited. Many fundamental biological questions related to biosynthesis under high HCO3- conditions and regulation of HCO3- tolerance need to be answered to understand how algae balance cation-anion and cope with the osmotic stress in the cell. Answering these questions also allows developing strateg ...
... limited. Many fundamental biological questions related to biosynthesis under high HCO3- conditions and regulation of HCO3- tolerance need to be answered to understand how algae balance cation-anion and cope with the osmotic stress in the cell. Answering these questions also allows developing strateg ...
Nutrition Essentials: Protein
... Protein is a nutrient that is comprised of amino acids. Sometimes these amino acids are referred to as building blocks for protein. Each amino acid has the same basic structure and includes carbon, hydrogen, oxygen, nitrogen, and a unique side group. The side group is different on each amino acid an ...
... Protein is a nutrient that is comprised of amino acids. Sometimes these amino acids are referred to as building blocks for protein. Each amino acid has the same basic structure and includes carbon, hydrogen, oxygen, nitrogen, and a unique side group. The side group is different on each amino acid an ...
the specificity and stability of the triton
... extent of extraction. The only variation seen in extractability of the cytoskeletal framework was in the presence of high concentrations of protein and low concentrations of detergent; under these conditions lipid also failed to be extracted. Since the critical micelle concentration of Triton X-100 ...
... extent of extraction. The only variation seen in extractability of the cytoskeletal framework was in the presence of high concentrations of protein and low concentrations of detergent; under these conditions lipid also failed to be extracted. Since the critical micelle concentration of Triton X-100 ...
Issues in predicting protein function from sequence
... among scientists and the general population alike that knowledge derived from sequence information will precipitate numerous breakthroughs in treating disease. To live up to such expectations will be a tall order, because considerable obstacles remain to be surmounted, but this is a worthwhile chall ...
... among scientists and the general population alike that knowledge derived from sequence information will precipitate numerous breakthroughs in treating disease. To live up to such expectations will be a tall order, because considerable obstacles remain to be surmounted, but this is a worthwhile chall ...
Targeting of interleukin-2 to the periplasm of
... The induced E. coli pGF25 (met-IL-2), pFQ (ssOmpAIL-2) and pFP6 (ssPhoA-IL-2) were fractionated and separated into spheroplasts, periplasmic and membrane fractions (Fig. 4). The hybrid proteins, ssOmpA-IL-2 (16.9 kDa) or ssPhoA-IL-2 (16.7 kDa), were neither properly processed to the mature form of I ...
... The induced E. coli pGF25 (met-IL-2), pFQ (ssOmpAIL-2) and pFP6 (ssPhoA-IL-2) were fractionated and separated into spheroplasts, periplasmic and membrane fractions (Fig. 4). The hybrid proteins, ssOmpA-IL-2 (16.9 kDa) or ssPhoA-IL-2 (16.7 kDa), were neither properly processed to the mature form of I ...
Perspective
... mycobacteria, corynebacteria, nocarThe mycobacterial cell envelope showing the most important structural components. Passage of small hydrophilic molecules through the OM requires the involvement of dia, rhodococci, and several other genporins (top). The figure illustrates the covalent linkages betw ...
... mycobacteria, corynebacteria, nocarThe mycobacterial cell envelope showing the most important structural components. Passage of small hydrophilic molecules through the OM requires the involvement of dia, rhodococci, and several other genporins (top). The figure illustrates the covalent linkages betw ...
The use of isotope-coded affinity tags (ICAT)
... proteins. The extracted proteins were digested with trypsin and the resulting peptides were separated and analysed by reverse-phase capillary LC-MS/MS. Using this approach, 112 proteins were identified, of which 79% were envelope proteins, 15% were stromal or thylakoid and 6% were contaminants from ...
... proteins. The extracted proteins were digested with trypsin and the resulting peptides were separated and analysed by reverse-phase capillary LC-MS/MS. Using this approach, 112 proteins were identified, of which 79% were envelope proteins, 15% were stromal or thylakoid and 6% were contaminants from ...
No Slide Title
... co-incubation with O-glycosidase and sialidase A shifted the 22-kD species to a 16-kD position (31), where it now co-migrated with the unmodified S monomer, Sp16, from crude brain extracts (Fig. 5C). We conclude from our data obtained by mass spectrometry analysis (21) and these enzymatic digestion ...
... co-incubation with O-glycosidase and sialidase A shifted the 22-kD species to a 16-kD position (31), where it now co-migrated with the unmodified S monomer, Sp16, from crude brain extracts (Fig. 5C). We conclude from our data obtained by mass spectrometry analysis (21) and these enzymatic digestion ...
Different subcellular locations of secretome components of
... synthesis machinery, or the presence of lipid subdomains surrounding the transport systems. ...
... synthesis machinery, or the presence of lipid subdomains surrounding the transport systems. ...
Structural Basis for Type VI Secretion Effector Recognition
... information about the genetic requirements for T6S function and provided evidence that a subset of T6SSs influence pathogenesis by specifically mediating bacterial interactions with eukaryotic cells [10]. In addition to mediating host cell interactions, the T6SS has been shown to regulate gene expre ...
... information about the genetic requirements for T6S function and provided evidence that a subset of T6SSs influence pathogenesis by specifically mediating bacterial interactions with eukaryotic cells [10]. In addition to mediating host cell interactions, the T6SS has been shown to regulate gene expre ...
Activation of Lymphocyte Signaling by the R1 Protein of Rhesus
... CD8 in order to analyze the contributions of the various tyrosine-containing elements to B-cell signaling. Six CD8⌬-R1 chimera constructs were made (Fig. 2). The cytoplasmic deletion mutations were selected based on the types of SH2 binding motifs present in the R1 cytoplasmic domain. The last five ...
... CD8 in order to analyze the contributions of the various tyrosine-containing elements to B-cell signaling. Six CD8⌬-R1 chimera constructs were made (Fig. 2). The cytoplasmic deletion mutations were selected based on the types of SH2 binding motifs present in the R1 cytoplasmic domain. The last five ...
Brassinosteroid Signal Transduction from Receptor Kinases to
... amino acid residues deleted, displayed higher kinase activity in vitro and more complete rescue of the bri1 mutant phenotype in transgenic plants, suggesting an autoinhibitory role of the CT (92). The CT region contains multiple phosphorylation sites, and mutation of these Ser/Thr residues to phosph ...
... amino acid residues deleted, displayed higher kinase activity in vitro and more complete rescue of the bri1 mutant phenotype in transgenic plants, suggesting an autoinhibitory role of the CT (92). The CT region contains multiple phosphorylation sites, and mutation of these Ser/Thr residues to phosph ...
7.06 Problem Set #5, Spring 2005
... of protease treatment, that means that your protein was cleaved by the proteases. Thus part of your protein must have been exposed (not inside the microsomes) and susceptible to the proteases. That would indicate that the protein was a trans-membrane protein, because the cytosolic segment of the pr ...
... of protease treatment, that means that your protein was cleaved by the proteases. Thus part of your protein must have been exposed (not inside the microsomes) and susceptible to the proteases. That would indicate that the protein was a trans-membrane protein, because the cytosolic segment of the pr ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.