Differentially Expressed Soluble Proteins in Aortic Cells from
... were not identified on each gel. Only differentially expressed spots (WC vs. SR) identified by Phoretix Software were recorded. For these spots, the coefficient of variation was 22%. Selected differentially expressed spots were excised from gels, destained, subjected to trypsin digestion, and spotte ...
... were not identified on each gel. Only differentially expressed spots (WC vs. SR) identified by Phoretix Software were recorded. For these spots, the coefficient of variation was 22%. Selected differentially expressed spots were excised from gels, destained, subjected to trypsin digestion, and spotte ...
Interaction of the MAGUK family member Acvrinp1 and the
... The evolutionary conserved Notch signal transduction pathway regulates cell fate and cellular differentiation in various tissues and has essential functions in embryonic patterning and tumorigenesis. Cell-cell signaling by the Notch pathway is mediated by the interaction of the transmembrane recepto ...
... The evolutionary conserved Notch signal transduction pathway regulates cell fate and cellular differentiation in various tissues and has essential functions in embryonic patterning and tumorigenesis. Cell-cell signaling by the Notch pathway is mediated by the interaction of the transmembrane recepto ...
Unit 3 1 of 3
... A) Explain how redox reactions are used in cellular respiration & photosynthesis. B) Describe the general roles of dehydrogenases, NAD, NADP, and the electron transport chain in cellular respiration. II) Origins of Cellular Respiration A) Describe the conditions under which respiration originated B) ...
... A) Explain how redox reactions are used in cellular respiration & photosynthesis. B) Describe the general roles of dehydrogenases, NAD, NADP, and the electron transport chain in cellular respiration. II) Origins of Cellular Respiration A) Describe the conditions under which respiration originated B) ...
Oxidative Phosphorylation
... • Paul Boyer finally put the puzzle together by proposing that there must be three sites with different binding affinities for the substrate (ADP + Pi) and product (ATP). • In fact, the three β-subunits interact in such a way that when one assumes the β-empty form, its neighbor to one side must assu ...
... • Paul Boyer finally put the puzzle together by proposing that there must be three sites with different binding affinities for the substrate (ADP + Pi) and product (ATP). • In fact, the three β-subunits interact in such a way that when one assumes the β-empty form, its neighbor to one side must assu ...
Mapping functional regions of the segment
... contain acidic regions at similar locations within their N-terminal region, suggesting that the functional organization of these proteins has been conserved during evolution. INTRODUCTION The mouse gene Krox-20 was originally identified as a member of a subset of genes, termed immediate-early genes, ...
... contain acidic regions at similar locations within their N-terminal region, suggesting that the functional organization of these proteins has been conserved during evolution. INTRODUCTION The mouse gene Krox-20 was originally identified as a member of a subset of genes, termed immediate-early genes, ...
Amino Acid Jazz: Amplifying Biochemistry Concepts
... In principle, these benefits could apply to students of all ages. However, classification of existing science songs and musicbased lesson plans by grade level5,6 suggests that music is used infrequently in high school and college-level courses. One potential reason for this is that educational songs a ...
... In principle, these benefits could apply to students of all ages. However, classification of existing science songs and musicbased lesson plans by grade level5,6 suggests that music is used infrequently in high school and college-level courses. One potential reason for this is that educational songs a ...
Histone H3 Phosphorylation Is Coupled to Poly-(ADP
... that ultimately leads to oncotic cell death is premature chromatin condensation (PCC) and the ensuing mitotic catastrophe (Novak and Tyson, 1997). Because a variety of phosphatase inhibitors induce PCC (Coco-Martin and Begg, 1997), protein phosphorylation must play an important role in this process. ...
... that ultimately leads to oncotic cell death is premature chromatin condensation (PCC) and the ensuing mitotic catastrophe (Novak and Tyson, 1997). Because a variety of phosphatase inhibitors induce PCC (Coco-Martin and Begg, 1997), protein phosphorylation must play an important role in this process. ...
Membrane-enclosed Crystals in Dictyostelium discoideum Cells
... 1961; Maeda and Takeuchi, 1969), suggesting that the proteins constituting these crystals are encoded by developmentally regulated, early expressed genes. The crystals remain present throughout the stages after aggregation. They are even found in the mature spores and disappear only after their germ ...
... 1961; Maeda and Takeuchi, 1969), suggesting that the proteins constituting these crystals are encoded by developmentally regulated, early expressed genes. The crystals remain present throughout the stages after aggregation. They are even found in the mature spores and disappear only after their germ ...
Generation of polyclonal antiserum for the detection
... with the same peptide sequence containing non-methylated arginine residues by ELISA or western blot. AntimRG co-localizes in situ with the methylprotein, nucleolin, by immunocytochemical staining. Further tests of methyl specificity include demonstrations of antimRG binding to recombinant protein or ...
... with the same peptide sequence containing non-methylated arginine residues by ELISA or western blot. AntimRG co-localizes in situ with the methylprotein, nucleolin, by immunocytochemical staining. Further tests of methyl specificity include demonstrations of antimRG binding to recombinant protein or ...
View Full PDF - Biochemical Society Transactions
... of MP to plasmodesmata have been proposed [21], conclusive in vivo evidence for such a role remains to be demonstrated. Importantly, the co-expression of fluorescent-protein-tagged MP and PME during TMV infection or in transfected cells did not reveal any significant co-distribution of the two prote ...
... of MP to plasmodesmata have been proposed [21], conclusive in vivo evidence for such a role remains to be demonstrated. Importantly, the co-expression of fluorescent-protein-tagged MP and PME during TMV infection or in transfected cells did not reveal any significant co-distribution of the two prote ...
... (Terry) Chen, for their friendship and assistance in completing this project. I would like to thank Dr. Sheryl H. Ehrman, Dr. Srinivasa R. Raghavan, and Dr. William A. Weigand for serving on my committee. I would also like to thank my laboratory partners for their friendship and guidance during my g ...
Microbial Cell Surfaces and Secretion Systems
... conserved in nature and corresponds to the Sec61 complex in the endoplasmic reticulum of eukaryotes. Energy for export is provided by the motor protein SecA, which hydrolyzes ATP, and the proton-motive force. The Sec machinery also inserts proteins into the CM. CM proteins are generally not produced ...
... conserved in nature and corresponds to the Sec61 complex in the endoplasmic reticulum of eukaryotes. Energy for export is provided by the motor protein SecA, which hydrolyzes ATP, and the proton-motive force. The Sec machinery also inserts proteins into the CM. CM proteins are generally not produced ...
EnCor Biotechnology Inc
... cells, and which appeared to work on all vertebrates. A few neuronal cell types were not recognized by the the NeuN antibody, such as cerebellar Purkinje cells, olfactory Mitral cells and retinal photoreceptors, but no non neuronal cells were recognized. The vast majority of neurons are strongly Neu ...
... cells, and which appeared to work on all vertebrates. A few neuronal cell types were not recognized by the the NeuN antibody, such as cerebellar Purkinje cells, olfactory Mitral cells and retinal photoreceptors, but no non neuronal cells were recognized. The vast majority of neurons are strongly Neu ...
Document
... • proteins within the cell that assist with appropriate folding of proteins • their role is to prevent misfolding rather than actively direct correct folding • can act to delay any folding (e.g. as the nascent polypeptide chain emerges from the ribosome) • can also ‘rescue’ misfolded proteins to the ...
... • proteins within the cell that assist with appropriate folding of proteins • their role is to prevent misfolding rather than actively direct correct folding • can act to delay any folding (e.g. as the nascent polypeptide chain emerges from the ribosome) • can also ‘rescue’ misfolded proteins to the ...
UBIQUITIN AT FOX CHASE
... pivotal question asked of Urban was: Do you know any examples of two proteins that are linked covalently. This recalled the small protein of unknown function, ubiquitin, a covalent ligand of histone H2A. The size and amino acid composition of APF-l reported by Hershko and known for ubiquitin were in ...
... pivotal question asked of Urban was: Do you know any examples of two proteins that are linked covalently. This recalled the small protein of unknown function, ubiquitin, a covalent ligand of histone H2A. The size and amino acid composition of APF-l reported by Hershko and known for ubiquitin were in ...
Amino Acid Building Block Models – In Brief
... Amino acids are the building blocks of proteins. All amino acids have an identical core structure consisting of an alpha-carbon, carboxyl group, amino group and R-group (sidechain). A linear chain of amino acids is a polypeptide. The primary sequence of a protein is the linear sequence of amino acid ...
... Amino acids are the building blocks of proteins. All amino acids have an identical core structure consisting of an alpha-carbon, carboxyl group, amino group and R-group (sidechain). A linear chain of amino acids is a polypeptide. The primary sequence of a protein is the linear sequence of amino acid ...
Heavy Metals and Metalloids As a Cause for Protein Misfolding and
... organisms maintain metal homeostasis within physiological or sub-toxic levels, respectively, and utilize metal detoxification mechanisms [1,2]. The toxicity of a given metal depends on its physicochemical properties and ligand preferences. “Soft” transition metals, like cadmium and mercury, prefer s ...
... organisms maintain metal homeostasis within physiological or sub-toxic levels, respectively, and utilize metal detoxification mechanisms [1,2]. The toxicity of a given metal depends on its physicochemical properties and ligand preferences. “Soft” transition metals, like cadmium and mercury, prefer s ...
Amino Acid Molymod Brief
... Amino acids are the building blocks of proteins. All amino acids have an identical core structure consisting of an alpha-carbon, carboxyl group, amino group and R-group (sidechain). A linear chain of amino acids is a polypeptide. The primary sequence of a protein is the linear sequence of amino acid ...
... Amino acids are the building blocks of proteins. All amino acids have an identical core structure consisting of an alpha-carbon, carboxyl group, amino group and R-group (sidechain). A linear chain of amino acids is a polypeptide. The primary sequence of a protein is the linear sequence of amino acid ...
FUNCTIONAL INVESTIGATION OF AN RNA BINDING PROTEIN
... containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Immunofluorescence studies showed that the 92 kDa PIMT protein and PRIP proteins are colocalized in the nucleus. PIMT binds S-adenosyl-L-methion ...
... containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Immunofluorescence studies showed that the 92 kDa PIMT protein and PRIP proteins are colocalized in the nucleus. PIMT binds S-adenosyl-L-methion ...
Hans-Sigrist Stiftung.indd - The Hans Sigrist Foundation
... thus amenable to diffraction studies. Electron and X-ray diffraction studies carried out in collaboration with Lousie Serpell and her colleagues have very recently resulted in the determination of the first atomic level structure of an amyloid fibril. The interactions between amino acid sidechains o ...
... thus amenable to diffraction studies. Electron and X-ray diffraction studies carried out in collaboration with Lousie Serpell and her colleagues have very recently resulted in the determination of the first atomic level structure of an amyloid fibril. The interactions between amino acid sidechains o ...
Phytopathogen type III effector weaponry and their plant targets
... by HopU1 possibly decreases their ability to bind, stabilize or process RNA [41]. It is not known how this alteration of RNA-binding leads to the suppression of innate immunity or if it alters specific or general RNA metabolism. Nevertheless, the post-transcriptional control of RNA by T3Es represe ...
... by HopU1 possibly decreases their ability to bind, stabilize or process RNA [41]. It is not known how this alteration of RNA-binding leads to the suppression of innate immunity or if it alters specific or general RNA metabolism. Nevertheless, the post-transcriptional control of RNA by T3Es represe ...
Tree of Life: Prokaryotes and Eukaryotes
... • One-celled or many-celled, with compartments (e.g., a nucleus is present) • Genetic material in two to many linear, separate chromosomes in the nucleus • Normally two copies of each gene present in an individual in part of the life cycle • A eukaryote has about 50,000 genes on average • Much less ...
... • One-celled or many-celled, with compartments (e.g., a nucleus is present) • Genetic material in two to many linear, separate chromosomes in the nucleus • Normally two copies of each gene present in an individual in part of the life cycle • A eukaryote has about 50,000 genes on average • Much less ...
Supplementary Table VI. List of Deletions/Hypomorphic Alleles
... involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p ADP-ribosylation factor GTPase activating protein (ARF GAP), involved i ...
... involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p ADP-ribosylation factor GTPase activating protein (ARF GAP), involved i ...
Cardiac O-GlcNAc signaling is increased in hypertrophy and heart
... Cardiac O-GlcNAc signaling is increased in hypertrophy and heart failure. Physiol Genomics 44: 162–172, 2012. First published November 29, 2011; doi:10.1152/physiolgenomics.00016.2011.— Reversible protein O-GlcNAc modification has emerged as an essential intracellular signaling system in several tis ...
... Cardiac O-GlcNAc signaling is increased in hypertrophy and heart failure. Physiol Genomics 44: 162–172, 2012. First published November 29, 2011; doi:10.1152/physiolgenomics.00016.2011.— Reversible protein O-GlcNAc modification has emerged as an essential intracellular signaling system in several tis ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.