Alight-inducible organelle targeting system for dynamically
... After we demonstrated that this optogenetic system could be easily used to tune a protein’s function by recruiting it to or away from the localization where it is active, we next applied it to a more complex system, cell cycle control, where the key regulatory proteins act at multiple subcellular lo ...
... After we demonstrated that this optogenetic system could be easily used to tune a protein’s function by recruiting it to or away from the localization where it is active, we next applied it to a more complex system, cell cycle control, where the key regulatory proteins act at multiple subcellular lo ...
MicroReview Expression, secretion and antigenic - UvA-DARE
... the GSP. Secretion of the S-protein of Aeromonas hydrophila is dependent on protein SpsD, a homologue of the PulD family of secretion proteins which have been implicated in the formation of channels in the outer membrane (Pugsley, 1993; Thomas and Trust, 1995). Protein ApsE, sharing sequence homolog ...
... the GSP. Secretion of the S-protein of Aeromonas hydrophila is dependent on protein SpsD, a homologue of the PulD family of secretion proteins which have been implicated in the formation of channels in the outer membrane (Pugsley, 1993; Thomas and Trust, 1995). Protein ApsE, sharing sequence homolog ...
BIOCHEMICAL ADAPTATION
... number and type of weak bonds that are important to its conformation, ligand binding, or other properties, a change in temperature affects each protein/enzyme slightly differently with the potential to disrupt the integrated functioning of multicomponent pathways. For example, a temperature increase ...
... number and type of weak bonds that are important to its conformation, ligand binding, or other properties, a change in temperature affects each protein/enzyme slightly differently with the potential to disrupt the integrated functioning of multicomponent pathways. For example, a temperature increase ...
PDF - Oxford Academic - Oxford University Press
... daughter cell (10,11). In addition to these biological processes, cell comunication events are also affected by the localization of speci®c mRNAs. Consequently, mRNA localization at the synapse has been suggested as a means to increase neuronal plasticity by local translation of speci®c proteins (re ...
... daughter cell (10,11). In addition to these biological processes, cell comunication events are also affected by the localization of speci®c mRNAs. Consequently, mRNA localization at the synapse has been suggested as a means to increase neuronal plasticity by local translation of speci®c proteins (re ...
Two genes encoding protein phosphatase 2A
... The 5 -untranslated (UT), ORF and 3 -UT sequences of OsPP2A-1 are almost identical to the OsPP2Ac cDNA reported by Chang et al. (1999). In the 5 -UT region, a sequence identity of 94% over a stretch of 104 bp was observed. In the coding sequence, a 98% identity with several base differences resul ...
... The 5 -untranslated (UT), ORF and 3 -UT sequences of OsPP2A-1 are almost identical to the OsPP2Ac cDNA reported by Chang et al. (1999). In the 5 -UT region, a sequence identity of 94% over a stretch of 104 bp was observed. In the coding sequence, a 98% identity with several base differences resul ...
Optimization of Ammonium Sulfate Concentration for Purification of
... in many developing countries where health systems are not well equipped (Rigano and Walmsley, 2005). For use in plant molecular biofarming, the tobacco plant has several advantages over other plants, such as highly efficient transformation and regeneration, relatively short period for biomass producti ...
... in many developing countries where health systems are not well equipped (Rigano and Walmsley, 2005). For use in plant molecular biofarming, the tobacco plant has several advantages over other plants, such as highly efficient transformation and regeneration, relatively short period for biomass producti ...
Molecular Weight Estimation - Bio-Rad
... A well-characterized protein of known MW, bovine actin (~42 kD), and a 27 kD protein were separated by SDS-PAGE alongside the Precision Plus Protein WesternC standards, transferred to blots, and visualized by chemiluminescence detection (Figure 2). Electrophoretic Rf of the standards, actin, and the ...
... A well-characterized protein of known MW, bovine actin (~42 kD), and a 27 kD protein were separated by SDS-PAGE alongside the Precision Plus Protein WesternC standards, transferred to blots, and visualized by chemiluminescence detection (Figure 2). Electrophoretic Rf of the standards, actin, and the ...
Evolution of the eukaryotic membrane
... two homologous objects share common ancestry, i.e. were once the same object in the common ancestor of the organisms that are being considered (Reeck et al., 1987). This definition can be applied to appendages (e.g. mammalian arms and fins), organelles, proteins, genes, amino acids or even nucleotid ...
... two homologous objects share common ancestry, i.e. were once the same object in the common ancestor of the organisms that are being considered (Reeck et al., 1987). This definition can be applied to appendages (e.g. mammalian arms and fins), organelles, proteins, genes, amino acids or even nucleotid ...
IL-13 - York College of Pennsylvania
... IL-13 ligand has been experimentally attached to other proteins to produce a new fusion or ‘chimera’ protein. By successfully developing a fused protein consisting of more than one active part, researchers have been able to successfully develop a single protein with multiple functions and potential ...
... IL-13 ligand has been experimentally attached to other proteins to produce a new fusion or ‘chimera’ protein. By successfully developing a fused protein consisting of more than one active part, researchers have been able to successfully develop a single protein with multiple functions and potential ...
Alterations in oxidative phosphorylation complex proteins in the
... (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK activator MAPK kinase 6 (MKK6). This protectio ...
... (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK activator MAPK kinase 6 (MKK6). This protectio ...
CheA 3 is the missing phosphatase - National e
... • Model predicts levels of RR-P throughout simulated chemotactic responses. • Model predicts that the signal termination process should take over 4 seconds. • However, experimental data indicate that cells can complete their entire response to a short stimulus in less than 1 second. • Need faster Ch ...
... • Model predicts levels of RR-P throughout simulated chemotactic responses. • Model predicts that the signal termination process should take over 4 seconds. • However, experimental data indicate that cells can complete their entire response to a short stimulus in less than 1 second. • Need faster Ch ...
Gene Section PTPRJ (protein tyrosine phosphatase, receptor type, J)
... IA2beta, GLEPP1 and STEP) is monomeric in solution (Barr et al., 2009). The regulation of receptor-like protein tyrosine phosphatases is generally poorly understood. Sorby et al. have identified MatrigelTM (a preparation of extracellular matrix proteins) as a source of DEP-1 ligand(s) and agonist(s) ...
... IA2beta, GLEPP1 and STEP) is monomeric in solution (Barr et al., 2009). The regulation of receptor-like protein tyrosine phosphatases is generally poorly understood. Sorby et al. have identified MatrigelTM (a preparation of extracellular matrix proteins) as a source of DEP-1 ligand(s) and agonist(s) ...
University of Birmingham Armadillo repeat proteins: beyond the
... The first Arm repeat protein to be implicated in cytoskeletal regulation in multicellular organisms was b-catenin, a component of adherens junctions in animals. Adherens junctions are points of intercellular contact, where cadherin molecules at the plasma membrane are anchored to the actin cytoskele ...
... The first Arm repeat protein to be implicated in cytoskeletal regulation in multicellular organisms was b-catenin, a component of adherens junctions in animals. Adherens junctions are points of intercellular contact, where cadherin molecules at the plasma membrane are anchored to the actin cytoskele ...
The Bacterial Phosphotransferase System: Structure, Function
... essential catalytic entities, termed Enzyme I, Enzyme II and HPr (heat-stable, histidine-phosphorylatable protein). The discovery of this system provided an explanation for pleiotropic carbohydrate-negative mutants of E. coli described as early as 1949 (Doudoroff et al., 1949). In 1964, the three re ...
... essential catalytic entities, termed Enzyme I, Enzyme II and HPr (heat-stable, histidine-phosphorylatable protein). The discovery of this system provided an explanation for pleiotropic carbohydrate-negative mutants of E. coli described as early as 1949 (Doudoroff et al., 1949). In 1964, the three re ...
PDF - Oxford Academic
... (Grafi et al., 1996; Xie et al., 1996; Ach et al., 1997). The list of cloned plant RBR genes is continuously expanding, and the phylogenetic tree reveals significant differences between dicot and monocot plant species as far as the number of RBR genes is concerned. While dicot plants only have a sin ...
... (Grafi et al., 1996; Xie et al., 1996; Ach et al., 1997). The list of cloned plant RBR genes is continuously expanding, and the phylogenetic tree reveals significant differences between dicot and monocot plant species as far as the number of RBR genes is concerned. While dicot plants only have a sin ...
The role of protein–protein interactions in the intracellular traffic of
... PPI because their biophysical properties (indicating their conformational state) and their copy number at the cell surface can be studied with high resolution using the patch-clamp technique [35]. Focusing on ion channels, numerous examples can be found where PPI are essential for their physiologica ...
... PPI because their biophysical properties (indicating their conformational state) and their copy number at the cell surface can be studied with high resolution using the patch-clamp technique [35]. Focusing on ion channels, numerous examples can be found where PPI are essential for their physiologica ...
Stress signaling from the lumen of the endoplasmic reticulum
... rebind calnexin and/or calreticulin and, in this manner, unfolded proteins are retained in the ER through a cycle of calnexin and calreticulin interaction, glucosidase II activity, and GT activity. Diversity in signaling from the ER lumen In addition to glycoprotein folding and assembly, the ER is a ...
... rebind calnexin and/or calreticulin and, in this manner, unfolded proteins are retained in the ER through a cycle of calnexin and calreticulin interaction, glucosidase II activity, and GT activity. Diversity in signaling from the ER lumen In addition to glycoprotein folding and assembly, the ER is a ...
A protein domain interaction interface database: InterPare | BMC
... whole protein configuration, can be viewed with three different molecular viewers on the results page. They are the Chime [24], Jmol [25], and InterFacer [26]. InterPare also provides the atomic coordinate files for protein surface, interior, and interface for further analysis. ...
... whole protein configuration, can be viewed with three different molecular viewers on the results page. They are the Chime [24], Jmol [25], and InterFacer [26]. InterPare also provides the atomic coordinate files for protein surface, interior, and interface for further analysis. ...
Inner nuclear membrane protein transport is mediated by multiple
... The contradictions in the studies published to date indicate that the targeting of INM proteins is much more complex than first assumed. Either a heretofore unclear mechanism exists that can somehow account for all these data or multiple translocation mechanisms exist and each individual INM protein ...
... The contradictions in the studies published to date indicate that the targeting of INM proteins is much more complex than first assumed. Either a heretofore unclear mechanism exists that can somehow account for all these data or multiple translocation mechanisms exist and each individual INM protein ...
Improved topology prediction using the terminal
... strategy is useful for TM-protein identification, it does not solve the problem of falsely identifying TM helices in non-TM domains of a TM-protein. In addition to the more hydrophobic N-terminal helix, we recently found that the C-terminal TM-helix is also more hydrophobic than central TM-helices ( ...
... strategy is useful for TM-protein identification, it does not solve the problem of falsely identifying TM helices in non-TM domains of a TM-protein. In addition to the more hydrophobic N-terminal helix, we recently found that the C-terminal TM-helix is also more hydrophobic than central TM-helices ( ...
PDF Copy - Brandon S. Russell, Ph.D.
... cells, they first showed that Aha is not the only amino acid analogue that can be used in BONCAT by looking at incorporation of the structurally similar homopropargylglycine (Hpg). As seen in Figure 2, Hpg is closely related to both methionine and Aha, and it too is translated by native protein mach ...
... cells, they first showed that Aha is not the only amino acid analogue that can be used in BONCAT by looking at incorporation of the structurally similar homopropargylglycine (Hpg). As seen in Figure 2, Hpg is closely related to both methionine and Aha, and it too is translated by native protein mach ...
The Dock and Lock Method: A Novel
... human RIIa and the AD of a certain amino acid sequence as an excellent pair of linker modules for docking any two entities, referred to hereafter as A and B, into a noncovalent complex, which could be further locked into a stably tethered structure through the introduction of cysteine residues into ...
... human RIIa and the AD of a certain amino acid sequence as an excellent pair of linker modules for docking any two entities, referred to hereafter as A and B, into a noncovalent complex, which could be further locked into a stably tethered structure through the introduction of cysteine residues into ...
T-cell regulator RNF125/TRAC-1 belongs to a novel family of
... E6-associated protein C-terminus) and U-box domains are the best characterized [9,10]. In agreement with their role in recruiting selected sets of substrates, the ubiquitin ligases comprise a large and heterogeneous group. It is estimated that > 400 are encoded in mammalian genomes [11], the majorit ...
... E6-associated protein C-terminus) and U-box domains are the best characterized [9,10]. In agreement with their role in recruiting selected sets of substrates, the ubiquitin ligases comprise a large and heterogeneous group. It is estimated that > 400 are encoded in mammalian genomes [11], the majorit ...
Response of Primary Human Airway Epithelial Cells to Influenza
... alkylation, and the tubes were incubated for a further 30 min (room temperature, in the dark). Finally, 100 mM DTT was added to quench the excess iodoacetic acid. Samples were digested overnight at 37 °C with 6 μg of sequencing grade trypsin (Promega, Madison, WI) and then stored at −80 °C until fur ...
... alkylation, and the tubes were incubated for a further 30 min (room temperature, in the dark). Finally, 100 mM DTT was added to quench the excess iodoacetic acid. Samples were digested overnight at 37 °C with 6 μg of sequencing grade trypsin (Promega, Madison, WI) and then stored at −80 °C until fur ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.