Structure and Function of Membrane Proteins: Overview
... A Brief History of Studies on the Structure of the Plasma Membrane I. Ernest Overton, University of Zurich (1890s) - knew that nonpolar solutes dissolve more readily in nonpolar solvents than in polar ones & that polar solutes are most soluble in polar solvents A. Since he realized that to enter a c ...
... A Brief History of Studies on the Structure of the Plasma Membrane I. Ernest Overton, University of Zurich (1890s) - knew that nonpolar solutes dissolve more readily in nonpolar solvents than in polar ones & that polar solutes are most soluble in polar solvents A. Since he realized that to enter a c ...
Excitation-contraction coupling in cardiomyocytes
... Na/Ca exchanger mechanism is essential in myocardial intracellular Ca regulation Na/Ca exchange is the main means by which Ca (entering the cell via L-type Ca channels) is extruded from the cell, during both relaxation & diastole. By comparison the sarcolemmal Ca-pump (SLCP) seems relatively unimpor ...
... Na/Ca exchanger mechanism is essential in myocardial intracellular Ca regulation Na/Ca exchange is the main means by which Ca (entering the cell via L-type Ca channels) is extruded from the cell, during both relaxation & diastole. By comparison the sarcolemmal Ca-pump (SLCP) seems relatively unimpor ...
The first cell fate specification event in mouse development
... nucleus is hyperacetylated and hypomethylated, two features that allow for higher accessibility to transcription factors and transcriptional activation (Hamatani et al., 2004; Li et al., 2010; Sch ...
... nucleus is hyperacetylated and hypomethylated, two features that allow for higher accessibility to transcription factors and transcriptional activation (Hamatani et al., 2004; Li et al., 2010; Sch ...
Nitric Oxide Signalling in Plants: Cross
... For instance, the skeletal muscle RyR1 (one of the three isoforms of RYRs) consists of four homologous 565 kDa subunits containing 100 cysteine residues. In the native protein, 50 of these residues appear to be in a reduced state (Aracena-Parks et al., 2006). Remarkably, submicromolar NO concentrati ...
... For instance, the skeletal muscle RyR1 (one of the three isoforms of RYRs) consists of four homologous 565 kDa subunits containing 100 cysteine residues. In the native protein, 50 of these residues appear to be in a reduced state (Aracena-Parks et al., 2006). Remarkably, submicromolar NO concentrati ...
Twins take the job
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
The connection of cytoskeletal network with plasma membrane and
... microtubules and the plasma membrane. However, tubulin subunits are commonly detected in immunoprecipitation assays, and additional microtubule localizations of PLDs in plants have not been reported. Treatment with 1-butanol of Arabidopsis roots (Motes et al. 2005) and membrane ghosts (Hirase et al. ...
... microtubules and the plasma membrane. However, tubulin subunits are commonly detected in immunoprecipitation assays, and additional microtubule localizations of PLDs in plants have not been reported. Treatment with 1-butanol of Arabidopsis roots (Motes et al. 2005) and membrane ghosts (Hirase et al. ...
Regulated adhesion as a driving force of gastrulation movements
... often used to measure relative ‘adhesiveness’ between cells, because cells with higher affinity end up in the centre of reaggregates, surrounded by cells with lower affinity (Steinberg, 2007). Several variants of the assay exist, for instance, incubating cells in ‘hanging drops’ or re-transplanting ...
... often used to measure relative ‘adhesiveness’ between cells, because cells with higher affinity end up in the centre of reaggregates, surrounded by cells with lower affinity (Steinberg, 2007). Several variants of the assay exist, for instance, incubating cells in ‘hanging drops’ or re-transplanting ...
Signal Perception and Transduction: The Origin of
... of this network could best be regarded as a prime example of chaos theory in action. A map can certainly be constructed of the network and its possible cross-links. However, although we can measure (and thus predict) the flux through parts of the network under precisely defined conditions, these pre ...
... of this network could best be regarded as a prime example of chaos theory in action. A map can certainly be constructed of the network and its possible cross-links. However, although we can measure (and thus predict) the flux through parts of the network under precisely defined conditions, these pre ...
The Expanding Role of the mammalian Target of Rapamycin
... inhibition by rapamycin in clinical trials [27]. Recently, a newly developed class of mTOR inhibitors, termed the ATP-competitive mTOR kinase inhibitors, have been shown to inhibit both mTORC1 and mTORC2 complexes [28,29]. These mTOR kinase inhibitors are currently under investigation in clinical tr ...
... inhibition by rapamycin in clinical trials [27]. Recently, a newly developed class of mTOR inhibitors, termed the ATP-competitive mTOR kinase inhibitors, have been shown to inhibit both mTORC1 and mTORC2 complexes [28,29]. These mTOR kinase inhibitors are currently under investigation in clinical tr ...
Protists
... back to about 1.2 billion years ago compared to bacteria which appear about 3.5 billion years ago. We do not know how the various groups of protists are related to one another. We assume they arose from certain groups of bacteria, but when and how they came into being, scientists are still invest ...
... back to about 1.2 billion years ago compared to bacteria which appear about 3.5 billion years ago. We do not know how the various groups of protists are related to one another. We assume they arose from certain groups of bacteria, but when and how they came into being, scientists are still invest ...
Cadherins constitute a superfamily of transmembrane - HAL
... Junctional assembly is a complex process which requires various events such as the local concentration of cadherin molecules at the cell surface, the recruitment of both cytoskeleton binding proteins and regulating proteins and the reorganization of cytoskeleton network. All these processes are inti ...
... Junctional assembly is a complex process which requires various events such as the local concentration of cadherin molecules at the cell surface, the recruitment of both cytoskeleton binding proteins and regulating proteins and the reorganization of cytoskeleton network. All these processes are inti ...
At the border: the plasma membrane–cell wall
... Other proteins, including integrin-like and receptor like kinases, such as WAKs, are attached to the plasma membrane through transmembrane domains. The ability of these proteins to bind to the cell wall relies on the presence of cellulose-binding domains (in COBRA) or arabinogalactan moieties which ...
... Other proteins, including integrin-like and receptor like kinases, such as WAKs, are attached to the plasma membrane through transmembrane domains. The ability of these proteins to bind to the cell wall relies on the presence of cellulose-binding domains (in COBRA) or arabinogalactan moieties which ...
Vacuolar transporters and their essential role in plant metabolism
... during the night. Remarkably, even a single vacuole may also be subdivided and fulfil several functions. This has been shown for protein storage vacuoles, where internal membranes separate storage proteins from both phytic acid and proteins that are characteristic of lytic vacuoles (Jiang et al., 20 ...
... during the night. Remarkably, even a single vacuole may also be subdivided and fulfil several functions. This has been shown for protein storage vacuoles, where internal membranes separate storage proteins from both phytic acid and proteins that are characteristic of lytic vacuoles (Jiang et al., 20 ...
Protein transport across the endoplasmic reticulum membrane
... Saccharomyces cerevisiae and E. coli and have only moderate translocation defects [32–34]. However, the crystal structures of these mutants show that new plugs are formed from neighboring polypeptide segments [34]. The new plugs still seal the closed channel, but they have lost many interactions tha ...
... Saccharomyces cerevisiae and E. coli and have only moderate translocation defects [32–34]. However, the crystal structures of these mutants show that new plugs are formed from neighboring polypeptide segments [34]. The new plugs still seal the closed channel, but they have lost many interactions tha ...
At the border: the plasma membrane–cell wall continuum
... that the CSCs are delivered to DRM microdomains. Lipid analysis revealed that DRMs are enriched in sterols and sphingolipids (Borner et al., 2005; Lefebvre et al., 2007). Sterols are one of the most important regulators of plasma membrane microdomain maintenance (Zauber et al., 2014). Perturbations ...
... that the CSCs are delivered to DRM microdomains. Lipid analysis revealed that DRMs are enriched in sterols and sphingolipids (Borner et al., 2005; Lefebvre et al., 2007). Sterols are one of the most important regulators of plasma membrane microdomain maintenance (Zauber et al., 2014). Perturbations ...
Translocation of Magnaporthe oryzae Effectors into
... fluorescent protein. These proteins accumulated in a novel structure, the biotrophic interfacial complex (BIC). BIC development is coupled to hyphal differentiation from filamentous to pseudohyphal (Veses and Gow, 2009) bulbous IH growth, which is required for disease development. PWL2 and BAS1, pat ...
... fluorescent protein. These proteins accumulated in a novel structure, the biotrophic interfacial complex (BIC). BIC development is coupled to hyphal differentiation from filamentous to pseudohyphal (Veses and Gow, 2009) bulbous IH growth, which is required for disease development. PWL2 and BAS1, pat ...
PDF
... stacks. The Golgi cisternae appear abnormally curved and numerous large vesicles are apparent (A, × 45 000; B, × 64 000). Fig. 7. Localisation of the JIM 84 epitope in 50 µg/ml BFA-treated maize root cells. (A) Immunogold labelling on LRWhite ultrathin sections: gold labelling of the perturbed Golgi ...
... stacks. The Golgi cisternae appear abnormally curved and numerous large vesicles are apparent (A, × 45 000; B, × 64 000). Fig. 7. Localisation of the JIM 84 epitope in 50 µg/ml BFA-treated maize root cells. (A) Immunogold labelling on LRWhite ultrathin sections: gold labelling of the perturbed Golgi ...
Podosome-type adhesions and focal adhesions, so alike yet so
... Focal complexes are 0.5-1 µm dot-like contacts localized along the lamellipodia. These structures are not connected to stress fibers although they have been shown to be linked to the actin network. Moreover, they do not contain Zyxin suggesting that they are subjected to moderate mechanical tensions ...
... Focal complexes are 0.5-1 µm dot-like contacts localized along the lamellipodia. These structures are not connected to stress fibers although they have been shown to be linked to the actin network. Moreover, they do not contain Zyxin suggesting that they are subjected to moderate mechanical tensions ...
Mutations in Actin-Related Proteins 2 and 3 Affect Cell Shape
... WRM and DIS1 Genes Are Integral Components of the ARP2/3 Complex Based on the mutations, the protein truncations in wrm1-2, dis1-1, and dis1-2 would be predicted to occur after amino acids 14, 97, and 290, respectively (Figure 1C). The G-to-A change in wrm1-1 results in the alteration of a conserved ...
... WRM and DIS1 Genes Are Integral Components of the ARP2/3 Complex Based on the mutations, the protein truncations in wrm1-2, dis1-1, and dis1-2 would be predicted to occur after amino acids 14, 97, and 290, respectively (Figure 1C). The G-to-A change in wrm1-1 results in the alteration of a conserved ...
Intracellular Protein Degradation
... platform for drug targeting, and mechanism-based drugs are currently developed, one of them is already on the market. The lysosome and intracellular protein degradation In the mid-1950s, Christian de Duve discovered the lysosome (see, for example, Refs. 8 and 9 and Figure 1).The lysosome was first r ...
... platform for drug targeting, and mechanism-based drugs are currently developed, one of them is already on the market. The lysosome and intracellular protein degradation In the mid-1950s, Christian de Duve discovered the lysosome (see, for example, Refs. 8 and 9 and Figure 1).The lysosome was first r ...
19-1 Bacteria
... • They are prokaryotes that produce methane gas. They live in oxygen-free environments, such as thick mud and the digestive tracts of animals. ...
... • They are prokaryotes that produce methane gas. They live in oxygen-free environments, such as thick mud and the digestive tracts of animals. ...
Quaternary ammonium surfactant structure determines selective
... different sensitivities to the harmful effects of QAS at concentrations below the CMC may arise from distinct chemical composition, physical properties and physiological functions of pathogens and host cell membranes, as well as the total amount of membrane per cell.20,22 Therefore, understanding QA ...
... different sensitivities to the harmful effects of QAS at concentrations below the CMC may arise from distinct chemical composition, physical properties and physiological functions of pathogens and host cell membranes, as well as the total amount of membrane per cell.20,22 Therefore, understanding QA ...
Cytosol
The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.