Small airway-on-a-chip enables analysis of human lung
... adhesion to ICAM-1 (ref: 16) and this dynamic shear stress-dependent response cannot be studied in a physiologically relevant way using static cell cultures. Advances in microsystems engineering have recently made it possible to create biomimetic microfluidic cell culture devices, known as ‘organs-o ...
... adhesion to ICAM-1 (ref: 16) and this dynamic shear stress-dependent response cannot be studied in a physiologically relevant way using static cell cultures. Advances in microsystems engineering have recently made it possible to create biomimetic microfluidic cell culture devices, known as ‘organs-o ...
Characterization of Cytosolic Cyclophilin from Guard Cells of Vicia
... Stomatal pores surrounded by guard cells regulate exchanges of CO2 and water between leaves and the atmosphere (Zeiger 1983, Assmann 1993). Since guard cells respond to various environmental stimuli such as light, humidity, CO2, Ca 2+ , and plant hormones, guard cells have been extensively investiga ...
... Stomatal pores surrounded by guard cells regulate exchanges of CO2 and water between leaves and the atmosphere (Zeiger 1983, Assmann 1993). Since guard cells respond to various environmental stimuli such as light, humidity, CO2, Ca 2+ , and plant hormones, guard cells have been extensively investiga ...
Functional analysis of cardiomyocytes carrying mutations in SCN5A
... 2.1 Introduction to cardiac muscle and cardiomyocytes 2.1.1 The structure of heart and cardiac cells The cardiac muscle, myocardium can be divided into four chambers: left atrium, left ventricle, right atrium and right ventricle (Figure 1) (Bjålie et al. 2008; Heikkilä et al. 2008). It has four valv ...
... 2.1 Introduction to cardiac muscle and cardiomyocytes 2.1.1 The structure of heart and cardiac cells The cardiac muscle, myocardium can be divided into four chambers: left atrium, left ventricle, right atrium and right ventricle (Figure 1) (Bjålie et al. 2008; Heikkilä et al. 2008). It has four valv ...
Auxin Auxins are a class of plant growth substance (often called
... which means that the auxin produced by the apical bud (or growing tip) diffuses downwards and inhibits the development of ulterior lateral bud growth, which would otherwise compete with the apical tip for light and nutrients. Removing the apical tip and its suppressive hormone allows the lower dorma ...
... which means that the auxin produced by the apical bud (or growing tip) diffuses downwards and inhibits the development of ulterior lateral bud growth, which would otherwise compete with the apical tip for light and nutrients. Removing the apical tip and its suppressive hormone allows the lower dorma ...
Biomaterials Science
... (Ingraham et al.). The paper pointed out that polyethylene production using a new high pressure polymerization technique began in 1936. This process enabled the production of polyethylene free of initiator fragments and other additives. Ingraham et al. demonstrated good results on implantation (i.e. ...
... (Ingraham et al.). The paper pointed out that polyethylene production using a new high pressure polymerization technique began in 1936. This process enabled the production of polyethylene free of initiator fragments and other additives. Ingraham et al. demonstrated good results on implantation (i.e. ...
Targeting CD45RB alters T cell migration and delays viral clearance
... CD8+ T cell recall response during secondary infection (5–8). As we have focused on the primary infection, we have concentrated our investigations on CD8+ T cells. CD45 is expressed on all nucleated hematopoietic cells and is a receptor tyrosine phosphatase critical for antigen-specific B and T cell ...
... CD8+ T cell recall response during secondary infection (5–8). As we have focused on the primary infection, we have concentrated our investigations on CD8+ T cells. CD45 is expressed on all nucleated hematopoietic cells and is a receptor tyrosine phosphatase critical for antigen-specific B and T cell ...
Extravasation Injury of the Upper Limb by Intravenous Clindamycin
... subcutaneous space. All intravenous fluids can cause tissue injury following extravasation. However, certain substances such as cytotoxic or vasoactive drugs and hyperosmolar agents are associated with a greater risk of tissue injury. The complications may range from minor (ecchymosis, hematoma and ...
... subcutaneous space. All intravenous fluids can cause tissue injury following extravasation. However, certain substances such as cytotoxic or vasoactive drugs and hyperosmolar agents are associated with a greater risk of tissue injury. The complications may range from minor (ecchymosis, hematoma and ...
The Proteome of Native Adult Müller Glial Cells From Murine
... dedifferentiation. A price for obtaining relatively pure Müller cells at high numbers, the cells gain stem cell-like characteristics after a few days in culture (15). Thus, expression profiles from cultured Müller glia do not adequately mirror those of Müller cells in native tissue. Accordingly, ...
... dedifferentiation. A price for obtaining relatively pure Müller cells at high numbers, the cells gain stem cell-like characteristics after a few days in culture (15). Thus, expression profiles from cultured Müller glia do not adequately mirror those of Müller cells in native tissue. Accordingly, ...
Annual Report 2014 The Kidney Project
... compatibility. We also developed thin film coatings to enhance blood biocompatibility and conducted successful short-term testing of blood compatibility of small implanted prototypes in rats and pigs. For the cell bioreactor, recent advances in the field of tissue engineering are being leveraged to ...
... compatibility. We also developed thin film coatings to enhance blood biocompatibility and conducted successful short-term testing of blood compatibility of small implanted prototypes in rats and pigs. For the cell bioreactor, recent advances in the field of tissue engineering are being leveraged to ...
The Dictyostelium LvsA Protein is Localized on the Contractile
... cells. To determine whether this GFP-LvsA fusion protein was functional, cells were assessed for their ability to divide in suspension cultures. LvsA mutant cells are not able to grow in these conditions due to their cytokinesis defect (2). All cell lines grew at rates identical to wild-type cells, ...
... cells. To determine whether this GFP-LvsA fusion protein was functional, cells were assessed for their ability to divide in suspension cultures. LvsA mutant cells are not able to grow in these conditions due to their cytokinesis defect (2). All cell lines grew at rates identical to wild-type cells, ...
chapter2 467..477 - Caister Academic Press
... Ramati, 2000; Ruby, 1992), while alternating between free living and intracellular growth phases. The definition of the species name ‘‘Bdellovibrio’’ was originally based on this observable phenotype: the intraperiplasmic growth habit of the organism and its biphasic life history. It can be argued, ...
... Ramati, 2000; Ruby, 1992), while alternating between free living and intracellular growth phases. The definition of the species name ‘‘Bdellovibrio’’ was originally based on this observable phenotype: the intraperiplasmic growth habit of the organism and its biphasic life history. It can be argued, ...
Human Adipose Stem Cells Current Clinical Applications
... The immunomodulatory properties of adipose-derived stem cells have been demonstrated in vitro and in vivo.30 –33 The correlations of the data sets derived from these models are currently being tested in several clinical studies to be reviewed later. There is still concern regarding adipose-derived s ...
... The immunomodulatory properties of adipose-derived stem cells have been demonstrated in vitro and in vivo.30 –33 The correlations of the data sets derived from these models are currently being tested in several clinical studies to be reviewed later. There is still concern regarding adipose-derived s ...
Autocrine Effects of Fibroblast Growth Factor in Repair of Radiation
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
A novel giant peroxisomal superoxide dismutase motif
... Total RNA (5 μg) from S, S+, C+, A, and A+ cells grown under the same conditions as used for the generation of the subtracted libraries were separated by denaturing 1% agarose–formaldehyde gel electrophoresis and transferred to a nylon membrane. Radiolabeled probes were generated by linear PCR as de ...
... Total RNA (5 μg) from S, S+, C+, A, and A+ cells grown under the same conditions as used for the generation of the subtracted libraries were separated by denaturing 1% agarose–formaldehyde gel electrophoresis and transferred to a nylon membrane. Radiolabeled probes were generated by linear PCR as de ...
Subordination
... Identify the passive sentences and change them to active • Lady was given her name by her mother. Being the only girl in a family of four, the name fit her well. She was raised by her mother and her two older brothers. They spent a great deal of family time together. She remembers going to all of h ...
... Identify the passive sentences and change them to active • Lady was given her name by her mother. Being the only girl in a family of four, the name fit her well. She was raised by her mother and her two older brothers. They spent a great deal of family time together. She remembers going to all of h ...
Cell Wall Architecture Prerequisite for the Cell
... of non-burst cells slightly increased at 10, 20 and 30 d upon suspension in medium containing 0.3 M or no mannitol. We separated divided from non-divided cells using a micromanipulation 10 d after the regeneration of protoplasts and then suspended the cells in different mannitol solutions. Neither c ...
... of non-burst cells slightly increased at 10, 20 and 30 d upon suspension in medium containing 0.3 M or no mannitol. We separated divided from non-divided cells using a micromanipulation 10 d after the regeneration of protoplasts and then suspended the cells in different mannitol solutions. Neither c ...
Loss of virus-specific CD4 T cells with increases in viral loads in the
... Follow-up of DNA-vaccinated macaques after SIV challenge In our previous study (Matano et al., 2000), two rhesus macaques (#20 and #21) that had been immunized with our DNA vaccine system using FMSIV and mCAT1 DNA were challenged intravenously with SIVmac239; both of them showed reduced plasma viral ...
... Follow-up of DNA-vaccinated macaques after SIV challenge In our previous study (Matano et al., 2000), two rhesus macaques (#20 and #21) that had been immunized with our DNA vaccine system using FMSIV and mCAT1 DNA were challenged intravenously with SIVmac239; both of them showed reduced plasma viral ...
The Sinorhizobium meliloti sensor histidine kinase
... to the potential importance of cell cycle regulation for establishing an effective host interaction. ...
... to the potential importance of cell cycle regulation for establishing an effective host interaction. ...
Cardiomyocytes from human pluripotent stem cells: From laboratory
... cases, adverse side effects lead to fatalities, as was the case with the serotonin agonist, cisparide, which caused 125 deaths before its use ceased [12]. 1.1. Current safety assessment platforms are suboptimal Underlying poor predictivity of cardiotoxicity are suboptimal safety assessment platforms ...
... cases, adverse side effects lead to fatalities, as was the case with the serotonin agonist, cisparide, which caused 125 deaths before its use ceased [12]. 1.1. Current safety assessment platforms are suboptimal Underlying poor predictivity of cardiotoxicity are suboptimal safety assessment platforms ...
Hormonal Control of Growth in Plants
... In a flower what happens to the ovule after fertilisation? It forms the seed! In a flower what happens to the ovary wall after fertilisation? It forms the fruit! Fruit formation is caused by high levels of IAA made by the developing embryo. ...
... In a flower what happens to the ovule after fertilisation? It forms the seed! In a flower what happens to the ovary wall after fertilisation? It forms the fruit! Fruit formation is caused by high levels of IAA made by the developing embryo. ...
Using light to shape chemical gradients for parallel and automated
... Parallel and automated analysis of chemotaxis phenotypes for gene perturbations We next applied our assay for high-throughput characterization of chemotaxis and motility phenotypes for gene perturbations. Highthroughput characterization and screening measurements are especially sensitive to experime ...
... Parallel and automated analysis of chemotaxis phenotypes for gene perturbations We next applied our assay for high-throughput characterization of chemotaxis and motility phenotypes for gene perturbations. Highthroughput characterization and screening measurements are especially sensitive to experime ...
Plant-microbe symbioses
... Nitrogen is an important element for living organisms as it is essential for the production of amino acids, proteins and nucleic acids. Although nitrogen is abundant in the atmosphere (78o/oof air without water vapour is nitrogen), plants cannot use it in its elemental form. Some soil bacteria (e.g. ...
... Nitrogen is an important element for living organisms as it is essential for the production of amino acids, proteins and nucleic acids. Although nitrogen is abundant in the atmosphere (78o/oof air without water vapour is nitrogen), plants cannot use it in its elemental form. Some soil bacteria (e.g. ...
Multiple Inducers of the Drosophila Heat Shock Locus 93D (hsro
... al., 1989). In each case the sequence analysis confirms the homology suggested by the phenotype of the puff. The hsroJ locus is expressed in nonstressed cells but the level of the transcripts is significantly increased by heat shock. In both stressed and control cells, three transcripts (Fig. 1) are ...
... al., 1989). In each case the sequence analysis confirms the homology suggested by the phenotype of the puff. The hsroJ locus is expressed in nonstressed cells but the level of the transcripts is significantly increased by heat shock. In both stressed and control cells, three transcripts (Fig. 1) are ...
6.cellandnucleardivision
... • Multicellular organisms depend on cell division for – Development from a fertilized cell – Growth ...
... • Multicellular organisms depend on cell division for – Development from a fertilized cell – Growth ...
Stem Cells of the Adult Olfactory Epithelium
... The peripheral olfactory system’s capacity to regenerate a population of neurons after injury has been known since the 1930s and 40s from both observations in humans (the recovery of olfactory function in children irrigated intranasally with zinc sulfate as prophylaxis against poliomyelitis) and exp ...
... The peripheral olfactory system’s capacity to regenerate a population of neurons after injury has been known since the 1930s and 40s from both observations in humans (the recovery of olfactory function in children irrigated intranasally with zinc sulfate as prophylaxis against poliomyelitis) and exp ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.