Designing cell lines for viral vaccine production: where do we stand?
... Finally, traditional cell lines still connotes adherent cells with a slow and tedious scale-up into bioreactors with microcarriers. Cells are often still grown under serum-containing ...
... Finally, traditional cell lines still connotes adherent cells with a slow and tedious scale-up into bioreactors with microcarriers. Cells are often still grown under serum-containing ...
Chapter 6 A Tour of the Cell
... A) plant cells are capable of having a much higher surface-to-volume ratio than animal cells. B) plant cells have a much more highly convoluted (folded) plasma membrane than animal cells. C) plant cells contain a large vacuole that reduces the volume of the cytoplasm. D) animal cells are more spheri ...
... A) plant cells are capable of having a much higher surface-to-volume ratio than animal cells. B) plant cells have a much more highly convoluted (folded) plasma membrane than animal cells. C) plant cells contain a large vacuole that reduces the volume of the cytoplasm. D) animal cells are more spheri ...
Nucleic Acid Synthesis in the Neoplastic Cell
... chemical (liffcre, mes 1)etween the D N A of normal and tumor tissues can bc expcrimentally demonstrated. Data on the molar basc composition, thc range of compositional heterogeneity, thc chromatographic heterogeneity, and the total cellular D N A content of tissues will bc reviewed. It will bc show ...
... chemical (liffcre, mes 1)etween the D N A of normal and tumor tissues can bc expcrimentally demonstrated. Data on the molar basc composition, thc range of compositional heterogeneity, thc chromatographic heterogeneity, and the total cellular D N A content of tissues will bc reviewed. It will bc show ...
The L5 epitope: an early marker for neural induction in the chick
... In some experiments, the tissue was digested in 20 mM sodium phosphate pH7.2, 10 mM EDTA, 40mM rc-octylglucoside containing 6 units of iV-glycosidase-F (peptide-N-glycosidase-F; E.C. 3.2.2.18; Boehringer-Mannheim) for every 100 fig of tissue digested, at 37°C for 18 h before immunoblotting as above. ...
... In some experiments, the tissue was digested in 20 mM sodium phosphate pH7.2, 10 mM EDTA, 40mM rc-octylglucoside containing 6 units of iV-glycosidase-F (peptide-N-glycosidase-F; E.C. 3.2.2.18; Boehringer-Mannheim) for every 100 fig of tissue digested, at 37°C for 18 h before immunoblotting as above. ...
Folliculin directs the formation of a Rab34–RILP
... lysosomes tended to be slightly larger and engaged in short saltatory movements (Fig 2Ai and Movie EV1 zoom panels), whereas peripheral FLCN-positive lysosomes moved rapidly along linear trajectories with velocities over 1 lm/s (Fig 2Aii and Movie EV1 zoom panels). We also noted FLCN-GFP association ...
... lysosomes tended to be slightly larger and engaged in short saltatory movements (Fig 2Ai and Movie EV1 zoom panels), whereas peripheral FLCN-positive lysosomes moved rapidly along linear trajectories with velocities over 1 lm/s (Fig 2Aii and Movie EV1 zoom panels). We also noted FLCN-GFP association ...
1)Yang, X., Dormann, D., Münsterberg, A. E., and Weijer - ICB-USP
... extraembryonic structures and all the structures of the embryo proper, while the hypoblast gives rise to extraembryonic structures only. Gastrulation starts with the formation of the primitive streak, which forms as a thickening of the epiblast at the posterior margin. The initial formation and ante ...
... extraembryonic structures and all the structures of the embryo proper, while the hypoblast gives rise to extraembryonic structures only. Gastrulation starts with the formation of the primitive streak, which forms as a thickening of the epiblast at the posterior margin. The initial formation and ante ...
Part 5-Inflammatory diseases, processes and outcomes
... chronic systemic inflammation, such as obesity, diabetes, inflammatory bowel disease, rheumatoid arthritis, and osteoarthritis, vascular diseases and even cancer and Alzheimerʼs disease. Lifestyle-related diseases are becoming more synonymous with chronic systemic inflammation because of an imbalanc ...
... chronic systemic inflammation, such as obesity, diabetes, inflammatory bowel disease, rheumatoid arthritis, and osteoarthritis, vascular diseases and even cancer and Alzheimerʼs disease. Lifestyle-related diseases are becoming more synonymous with chronic systemic inflammation because of an imbalanc ...
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... azole and polyene drugs (Canuto & Rodero, 2002) there is a requirement for new drugs with alternative modes of action or with the ability to increase the efficacy of existing prescription drugs. Many metalbased drugs display modes of action distinct to those of the prescription anti-fungals (Coyle e ...
... azole and polyene drugs (Canuto & Rodero, 2002) there is a requirement for new drugs with alternative modes of action or with the ability to increase the efficacy of existing prescription drugs. Many metalbased drugs display modes of action distinct to those of the prescription anti-fungals (Coyle e ...
Muscle Pathology
... Increase in endomysial connective tissue Nonspecific so-called dystrophic changes seen in many of the muscular dystrophies. Can also be seen in any chronic myopathic disorder. This disorder is due to loss of the protein emerin. ...
... Increase in endomysial connective tissue Nonspecific so-called dystrophic changes seen in many of the muscular dystrophies. Can also be seen in any chronic myopathic disorder. This disorder is due to loss of the protein emerin. ...
Document
... triggering crypt apoptosis and disrupting epithelial morphogenesis. Rab8a was also required for Cdc42-GTP activity in the intestinal epithelium, where continued cell division takes place. Furthermore, mice haploinsufficient for both Cdc42 and Rab8a in the intestine demonstrated abnormal crypt morpho ...
... triggering crypt apoptosis and disrupting epithelial morphogenesis. Rab8a was also required for Cdc42-GTP activity in the intestinal epithelium, where continued cell division takes place. Furthermore, mice haploinsufficient for both Cdc42 and Rab8a in the intestine demonstrated abnormal crypt morpho ...
Science Quarter 4 Lessons
... Metabolism- the sum of the physical and chemical processes in an organism Organization… An organism’s body must be organized in that enables it to meet its needs. Some organisms are simple: Bacteria Archaea Most Protists Some organisms are more complex: When different parts of the organism perform ...
... Metabolism- the sum of the physical and chemical processes in an organism Organization… An organism’s body must be organized in that enables it to meet its needs. Some organisms are simple: Bacteria Archaea Most Protists Some organisms are more complex: When different parts of the organism perform ...
PDF
... In normal development the first cleavage division cortical component of the mother cell (for example, produces daughter cells, AB and CD, that are unequal by Conklin, 1905; Boveri, 1910; Spemann, 1938; Shimizu, three criteria. Cell CD is larger than cell AB, it inherits 1982a, b; Milhausen and Agabi ...
... In normal development the first cleavage division cortical component of the mother cell (for example, produces daughter cells, AB and CD, that are unequal by Conklin, 1905; Boveri, 1910; Spemann, 1938; Shimizu, three criteria. Cell CD is larger than cell AB, it inherits 1982a, b; Milhausen and Agabi ...
PDF
... gain or loss of key pancreatic transcription factors, including Arx, Pax4 and Pdx1 (Collombat et al., 2007, 2009; Yang et al., 2011). Together, these studies have revealed plasticity in the endocrine pancreas and indicate that other pancreatic endocrine cells might be an exploitable source of new, f ...
... gain or loss of key pancreatic transcription factors, including Arx, Pax4 and Pdx1 (Collombat et al., 2007, 2009; Yang et al., 2011). Together, these studies have revealed plasticity in the endocrine pancreas and indicate that other pancreatic endocrine cells might be an exploitable source of new, f ...
Metabolism of lipids digestion, absorption, resynthesis in
... • are the largest lipoproteins (180 to 500 nm in diameter) • are synthesized in the ER of intestinal cells • contain 85 % of TGs (it is the main transport form of dietary TGs). • apoprotein B-48 (apo B-48) is the main protein component • deliver TGs from the intestine (via lymph and blood) to tissue ...
... • are the largest lipoproteins (180 to 500 nm in diameter) • are synthesized in the ER of intestinal cells • contain 85 % of TGs (it is the main transport form of dietary TGs). • apoprotein B-48 (apo B-48) is the main protein component • deliver TGs from the intestine (via lymph and blood) to tissue ...
laboratory science
... by the yellowed senescent lens may defend lipofuscinfilled RPE cells against blue light damage, we are interested in efforts being made to develop IOLs that replicate the transmission characteristics of the aging crystalline lens. In this study, we constructed a cell culture system that allowed us t ...
... by the yellowed senescent lens may defend lipofuscinfilled RPE cells against blue light damage, we are interested in efforts being made to develop IOLs that replicate the transmission characteristics of the aging crystalline lens. In this study, we constructed a cell culture system that allowed us t ...
PDF here - University of Edinburgh
... Next, while searching cytological literature for evidence of NMJcapping cell disposition, we noted the strong resemblance of 2166 antibody staining of the NMJ-capping cells to staining for neuregulin (GGFII) at NMJs, reported by Trinidad et al. (Trinidad et al., 2000). These authors used the HM-24 a ...
... Next, while searching cytological literature for evidence of NMJcapping cell disposition, we noted the strong resemblance of 2166 antibody staining of the NMJ-capping cells to staining for neuregulin (GGFII) at NMJs, reported by Trinidad et al. (Trinidad et al., 2000). These authors used the HM-24 a ...
The Care and Feeding of the Microscope Lab 5
... Magnification is the ratio of an object’s image size to its real size; magnification simply makes the objects or specimens appear larger. Resolution (resolving power) is the ability of an optical instrument to distinguish between two points that are close together, so that they are seen as separate ...
... Magnification is the ratio of an object’s image size to its real size; magnification simply makes the objects or specimens appear larger. Resolution (resolving power) is the ability of an optical instrument to distinguish between two points that are close together, so that they are seen as separate ...
The Id4 HLH protein and the timing of oligodendrocyte differentiation
... or purified (Barres et al., 1994) precursor cells isolated from postnatal day 7–8 (P7–8) optic nerve suggest that both a cell-intrinsic programme and extracellular signals play important parts in determining when the precursor cells stop dividing and differentiate. In the presence of appropriate sig ...
... or purified (Barres et al., 1994) precursor cells isolated from postnatal day 7–8 (P7–8) optic nerve suggest that both a cell-intrinsic programme and extracellular signals play important parts in determining when the precursor cells stop dividing and differentiate. In the presence of appropriate sig ...
Ferrara et al, Nat Med 2003 - Kashyap Memorial Eye Hospital
... • In both CRVO and BRVO, the development of new vessels and macular edema result in variable loss of vision • In one study, nearly 10% of eyes with BVRO had new vessels present and another 10% had macular edema present2 ...
... • In both CRVO and BRVO, the development of new vessels and macular edema result in variable loss of vision • In one study, nearly 10% of eyes with BVRO had new vessels present and another 10% had macular edema present2 ...
+ pdf
... Next, while searching cytological literature for evidence of NMJcapping cell disposition, we noted the strong resemblance of 2166 antibody staining of the NMJ-capping cells to staining for neuregulin (GGFII) at NMJs, reported by Trinidad et al. (Trinidad et al., 2000). These authors used the HM-24 a ...
... Next, while searching cytological literature for evidence of NMJcapping cell disposition, we noted the strong resemblance of 2166 antibody staining of the NMJ-capping cells to staining for neuregulin (GGFII) at NMJs, reported by Trinidad et al. (Trinidad et al., 2000). These authors used the HM-24 a ...
Human Skin Equivalent Model
... fluid loss, and immunity and sensory roles. Once damaged, skin cannot perform these normal tasks and numerous complications such as infection or fluid loss can occur1. To restore these roles, the body needs to close the wound as quickly as possible while retaining functionality and integrity. Our cu ...
... fluid loss, and immunity and sensory roles. Once damaged, skin cannot perform these normal tasks and numerous complications such as infection or fluid loss can occur1. To restore these roles, the body needs to close the wound as quickly as possible while retaining functionality and integrity. Our cu ...
Plant Vegetative Development: From Seed and Embryo to Shoot
... By contrast to shoot systems, the branching patterns of roots are not generated at the root apical meristem-lateral roots are initiated in the pericycle at some distance dista1 to the root tip (Malamy and Benfey, 1997). Instead, as Schiefelbein et al. discuss, activities at the root apical meristem ...
... By contrast to shoot systems, the branching patterns of roots are not generated at the root apical meristem-lateral roots are initiated in the pericycle at some distance dista1 to the root tip (Malamy and Benfey, 1997). Instead, as Schiefelbein et al. discuss, activities at the root apical meristem ...
Co-Infection with Mycobacterium tuberculosis Impairs HIV
... and 1B). HIV-specific CD4+ cell release of IFN-γ was significantly lower in HIV+/TB as compared to HIV+/LTBI (p = 0.005)(Fig. 1B). HIV-specific CD4+ cell release of TNF-α (p = 0.01) and IL-2 (p<0.001) were significantly lower in HIV+/TB as compared to HIV mono-infected individuals. In summary, HIV c ...
... and 1B). HIV-specific CD4+ cell release of IFN-γ was significantly lower in HIV+/TB as compared to HIV+/LTBI (p = 0.005)(Fig. 1B). HIV-specific CD4+ cell release of TNF-α (p = 0.01) and IL-2 (p<0.001) were significantly lower in HIV+/TB as compared to HIV mono-infected individuals. In summary, HIV c ...
Local opening of the DNA double helix in eukaryotic cells detected by
... bases in DNA segments that have non-B-DNA conformation (Paleček et al., 1987; Paleček, 1991). The Os,bipymodified bases can be detected after DNA isolation from the cells. However, the techniques that have been applied so far to intracellular plasmid DNAs (Paleček, 1991) cannot yield information ...
... bases in DNA segments that have non-B-DNA conformation (Paleček et al., 1987; Paleček, 1991). The Os,bipymodified bases can be detected after DNA isolation from the cells. However, the techniques that have been applied so far to intracellular plasmid DNAs (Paleček, 1991) cannot yield information ...
Article en format PDF - Université de Montréal
... © 2002 Nature Publishing Group http://biotech.nature.com ...
... © 2002 Nature Publishing Group http://biotech.nature.com ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.