Nondestructive Manipulation of Single Live Plant Cell by Laser
... attention as a novel biological technique. Some kind of single cell manipulation for both plant and animal cells have been demonstrated by using laser trapping and laser ablation (1-5). Recently, infrared laser as a source of trapping laser has become user-friendly machine with improving the device ...
... attention as a novel biological technique. Some kind of single cell manipulation for both plant and animal cells have been demonstrated by using laser trapping and laser ablation (1-5). Recently, infrared laser as a source of trapping laser has become user-friendly machine with improving the device ...
Expression of Growth Factor Receptors in
... a-chain MoAb, clone 17-A3' was purchased from PharMingen. Cells were first incubated for 60 minutes at 4°C in the presence of 2.5 mg/mL of this antibody, washed with cold PBS, and then incubated for 30 minutes at 4°C with biotin-conjugated sheep anti-mouse IgGs (Cappel, West Chester, PA). After wash ...
... a-chain MoAb, clone 17-A3' was purchased from PharMingen. Cells were first incubated for 60 minutes at 4°C in the presence of 2.5 mg/mL of this antibody, washed with cold PBS, and then incubated for 30 minutes at 4°C with biotin-conjugated sheep anti-mouse IgGs (Cappel, West Chester, PA). After wash ...
Relationship between the timing of DNA replication and the
... petence. On the basis of the results obtained with ND cells, it was suggested that the low encystation competence of D cells is due to a loss of accumulation of stationary stage D cells at the particular position of G 2 phase. This suggestion is supported by the finding that the nuclear DNA content ...
... petence. On the basis of the results obtained with ND cells, it was suggested that the low encystation competence of D cells is due to a loss of accumulation of stationary stage D cells at the particular position of G 2 phase. This suggestion is supported by the finding that the nuclear DNA content ...
The Boron Requirement and Cell Wall Properties
... Suspension-cultured Chenopodium album L. cells are capable of continuous, long-term growth on a boron-deficient medium. Compared with cultures grown with boron, these cultures contained more enlarged and detached cells, had increased turbidity due to the rupture of a small number of cells, and conta ...
... Suspension-cultured Chenopodium album L. cells are capable of continuous, long-term growth on a boron-deficient medium. Compared with cultures grown with boron, these cultures contained more enlarged and detached cells, had increased turbidity due to the rupture of a small number of cells, and conta ...
Triton X-100 Extraction of P815 Tumor Cells
... In contrast, many nucleated cells have extensive cytoskeletal systems that include microtubules, microfilaments, and intermediate filaments (13, 14). There is some evidence for associations between transmembrane proteins and these cytoskeletal elements but it has remained unclear whether the plasma ...
... In contrast, many nucleated cells have extensive cytoskeletal systems that include microtubules, microfilaments, and intermediate filaments (13, 14). There is some evidence for associations between transmembrane proteins and these cytoskeletal elements but it has remained unclear whether the plasma ...
a complexity drain on cells in the evolution of
... A second rationale is that selection on a multicellular entity favors a reduction in the range of behavioral possibilities in its component cells. To play its proper role, a cell must not only behave appropriately, it must be constrained from behaving inappropriately. One way to eliminate inappropri ...
... A second rationale is that selection on a multicellular entity favors a reduction in the range of behavioral possibilities in its component cells. To play its proper role, a cell must not only behave appropriately, it must be constrained from behaving inappropriately. One way to eliminate inappropri ...
Membrane trafficking and osmotically induced
... Both volume and medial area declined as external osmotic pressure was increased, and the relationship between these two parameters was found to be approximately linear over the range tested (Fig. 2). The heavy line is a linear regression of all points, and the lighter lines show linear regressions f ...
... Both volume and medial area declined as external osmotic pressure was increased, and the relationship between these two parameters was found to be approximately linear over the range tested (Fig. 2). The heavy line is a linear regression of all points, and the lighter lines show linear regressions f ...
Figure 1 - Journal of The Royal Society Interface
... The cellular structures of a representative softwood (cedar) and a hardwood (oak) are shown in figure 3. In softwoods, the bulk of the cells (85 – 95%) are highly elongated tracheids that provide both structural support and a conduction path for fluids (through small openings, called bordered pits, al ...
... The cellular structures of a representative softwood (cedar) and a hardwood (oak) are shown in figure 3. In softwoods, the bulk of the cells (85 – 95%) are highly elongated tracheids that provide both structural support and a conduction path for fluids (through small openings, called bordered pits, al ...
Signalling in Plant Lateral Organ Development
... to remain within the same cell layer and to differentiate as cells of the same type (e.g., as palisade mesophyll; Figure 1). Restricted orientation of division could therefore provide the basis for heritable cell identity within a layer. It also raises the possibility that cell identity might itself ...
... to remain within the same cell layer and to differentiate as cells of the same type (e.g., as palisade mesophyll; Figure 1). Restricted orientation of division could therefore provide the basis for heritable cell identity within a layer. It also raises the possibility that cell identity might itself ...
Functional differences between kindlin-1 and kindlin
... was no longer able to pull down kindlin-2, whereas the GSTb6b1 construct was able to pull down kindlin-2 as efficiently as GST–b1 was (Fig. 4G). Consistent with its ability to bind both b1- and b6-tails, the binding of kindlin-1 to GST–b6-b1 and GST–b1-b6 was not affected (Fig. 4F). To further narro ...
... was no longer able to pull down kindlin-2, whereas the GSTb6b1 construct was able to pull down kindlin-2 as efficiently as GST–b1 was (Fig. 4G). Consistent with its ability to bind both b1- and b6-tails, the binding of kindlin-1 to GST–b6-b1 and GST–b1-b6 was not affected (Fig. 4F). To further narro ...
cell cycle pp
... An example of an internal signal occurs at the M phase checkpoint In this case, anaphase does not begin if any kinetochores remain unattached to spindle microtubules Attachment of all of the kinetochores activates a regulatory complex, which then activates the enzyme separase Separase allow ...
... An example of an internal signal occurs at the M phase checkpoint In this case, anaphase does not begin if any kinetochores remain unattached to spindle microtubules Attachment of all of the kinetochores activates a regulatory complex, which then activates the enzyme separase Separase allow ...
Elevated IL-17 produced by TH17 cells promotes
... next observed that IL-17 promotes myeloma cell growth and colony formation via IL-17 receptor, adhesion to bone marrow stromal cells (BMSCs) as well as increased growth in vivo in murine xeno- ...
... next observed that IL-17 promotes myeloma cell growth and colony formation via IL-17 receptor, adhesion to bone marrow stromal cells (BMSCs) as well as increased growth in vivo in murine xeno- ...
Epithelial to mesenchymal transition during gastrulation
... process can be viewed as not to involve EMT, for the epithelial sheet is somewhat retained in the involuted endoderm. It is, however, unclear whether these cells still retain tight junctions. Some of the involuted cells located in the endoderm layer will later on contribute to the mesoderm through a ...
... process can be viewed as not to involve EMT, for the epithelial sheet is somewhat retained in the involuted endoderm. It is, however, unclear whether these cells still retain tight junctions. Some of the involuted cells located in the endoderm layer will later on contribute to the mesoderm through a ...
Alteration of Chemotaxis in the Gut of IBD Patients
... What is still unknown about neutrophil chemotaxis in IBD? Well, a lot of things. ECM proteins and their degradation products ...
... What is still unknown about neutrophil chemotaxis in IBD? Well, a lot of things. ECM proteins and their degradation products ...
TKRP125, a kinesin-related protein involved in the centrosome
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
PDF - Walter Lab
... from assembling into cortical actin patches (Fig. 4a), thus indicating that the Lat A treatment was effective. In contrast to untreated cells, however, FM4-64 foci in Lat-A-treated cells were not consumed even at late time points. Together, these results show that the formation of FM4-64 foci at the ...
... from assembling into cortical actin patches (Fig. 4a), thus indicating that the Lat A treatment was effective. In contrast to untreated cells, however, FM4-64 foci in Lat-A-treated cells were not consumed even at late time points. Together, these results show that the formation of FM4-64 foci at the ...
Skeletal Muscles
... with myosin. Troponin-I prevents actin from binding to myosin. Troponin-C binds to calcium ion. When this occurs, a conformational change occurs in the troponin complex, and myosin can now bind and interact with the actin filament. The energy needed for the cross bridging between myosin and actin is ...
... with myosin. Troponin-I prevents actin from binding to myosin. Troponin-C binds to calcium ion. When this occurs, a conformational change occurs in the troponin complex, and myosin can now bind and interact with the actin filament. The energy needed for the cross bridging between myosin and actin is ...
... lineage play a critical role in the effector phase of a number of phenomena of cell-mediated immunity: they can either enhance or suppress immune reactions [1]. It has been postulated that the macrophages act both via soluble monok:ines [2], and by cell-to-cell contact [3]. The down-regulation activ ...
Immunology of interstitial lung diseases: ... place in the lung of sarcoidosis, ...
... we evaluated adherent cells freshly recovered from the BAL for their capacity to produce the type IV collagenase. We demonstrated that sarcoid alveolar macrophages from patients with active sarcoidosis release significantly increased levels of type IV collagenase in vitro with respect to controls [1 ...
... we evaluated adherent cells freshly recovered from the BAL for their capacity to produce the type IV collagenase. We demonstrated that sarcoid alveolar macrophages from patients with active sarcoidosis release significantly increased levels of type IV collagenase in vitro with respect to controls [1 ...
PDF
... and since they are responsive to local changes in cell density (Davey and Zandstra, 2006), we decided to first investigate if early developmental markers would respond to changes in cell density and if so, how they would be regulated. We plated cells on gelatin-coated dishes in FCS and L ...
... and since they are responsive to local changes in cell density (Davey and Zandstra, 2006), we decided to first investigate if early developmental markers would respond to changes in cell density and if so, how they would be regulated. We plated cells on gelatin-coated dishes in FCS and L ...
Wang YY, Kuang A, Russell SD, Tian HQ. 2006. In vitro fertilization
... Sperm cells are expected to fulfill the functions of recognition, adherence and fusion with the female gamete during fertilization. Each of these functions of the male gamete may be controlled by activation of a unique gene or network of genes. The isolation of such fertilization-related genes in spe ...
... Sperm cells are expected to fulfill the functions of recognition, adherence and fusion with the female gamete during fertilization. Each of these functions of the male gamete may be controlled by activation of a unique gene or network of genes. The isolation of such fertilization-related genes in spe ...
Should reflectance confocal microscopy be the gold standard for
... tumor cells in the basal layer of the epidermis. These cells contain ellipsoidal nuclei, parallel to the overall polarity axis of the cell forming ‘palisades’ around less polarized tumor cells, which are often referred to as ‘tumor islands’. Tumor islands mostly contain basaloid cells and are mostly ...
... tumor cells in the basal layer of the epidermis. These cells contain ellipsoidal nuclei, parallel to the overall polarity axis of the cell forming ‘palisades’ around less polarized tumor cells, which are often referred to as ‘tumor islands’. Tumor islands mostly contain basaloid cells and are mostly ...
Full-Text PDF
... as a model vaccine. RSV is the main cause of lower respiratory tract infections in infants and the elderly, and also affects high-risk adults [14,15]. Although several strategies, including use of vaccines and therapeutic antibodies, have been tested for treatment of RSV infections in the last few d ...
... as a model vaccine. RSV is the main cause of lower respiratory tract infections in infants and the elderly, and also affects high-risk adults [14,15]. Although several strategies, including use of vaccines and therapeutic antibodies, have been tested for treatment of RSV infections in the last few d ...
Four new subunits of the Dam1Duo1 complex reveal novel functions
... It is during mitosis that sister chromatids, generated by chromosome duplication during S phase, are segregated to opposite poles by the mitotic spindle. Budding yeast Saccharomyces cerevisiae has been particularly useful for the dissection of mitotic events (Winey and O'Toole, 2001). In yeast, micr ...
... It is during mitosis that sister chromatids, generated by chromosome duplication during S phase, are segregated to opposite poles by the mitotic spindle. Budding yeast Saccharomyces cerevisiae has been particularly useful for the dissection of mitotic events (Winey and O'Toole, 2001). In yeast, micr ...
Designing cell lines for viral vaccine production: where do we stand?
... Finally, traditional cell lines still connotes adherent cells with a slow and tedious scale-up into bioreactors with microcarriers. Cells are often still grown under serum-containing ...
... Finally, traditional cell lines still connotes adherent cells with a slow and tedious scale-up into bioreactors with microcarriers. Cells are often still grown under serum-containing ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.