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Homeostatic proliferation generates long- lived natural killer
Homeostatic proliferation generates long- lived natural killer

... functional pool of immune cells ready to respond against foreign invaders. Homeostatic, or space-driven, proliferation of lymphocytes is an important mechanism that the immune system has evolved to maintain a sufficient number of mature hematopoietic cells to mount an effective response against path ...
Identification of novel MYO18A interaction partners - HAL
Identification of novel MYO18A interaction partners - HAL

... Correspondence and requests for materials should be addressed to Z.-G.X. (email: [email protected]) or D.-L.S. (email: [email protected]) Scientific Reports | 6:36768 | DOI: 10.1038/srep36768 ...
Drosophila asymmetric division, polarity and cancer - e
Drosophila asymmetric division, polarity and cancer - e

... positions during development and are in close contact with neighbouring cells: their most recent daughters on the basal side (Truman and Bate, 1988), and cortex glial cells on their apical and lateral sides (Dumstrei et al., 2003). Moreover, NBs are not insensitive to their neighbours and are definit ...
Stimulated release of fluorescently labeled IgE fragments that
Stimulated release of fluorescently labeled IgE fragments that

... fragments by aggregation of high affinity receptors for IgE (FcεRI) or by Ca2+ ionophore and phorbol 12-myristate 13acetate results in release of FITC fluorescence from the cells, which can be monitored continuously with a spectrofluorometer. The fluorescence release process corresponds to cellular ...
CpG-ODN 2006 and human parvovirus B19 genome consensus
CpG-ODN 2006 and human parvovirus B19 genome consensus

... 1Department of Hematology, Nephrology, and Rheumatology. Akita University Graduate School of Medicine, Akita, Japan; 2Department of Rheumatology, First Affiliated Hospital of China Medical University, Shenyang, China; 3Department of Medical Microbiology, Mycology and Immunology, Tohoku University Gr ...
Surface Sialic Acids Taken from the Host Allow Trypanosome
Surface Sialic Acids Taken from the Host Allow Trypanosome

... peptides are replaced by the intact GPI precursor, PP1 (Fig. 2 A; reference 26). The GPI-anchored procyclins and some excess PP1 leave the ER and are processed in the Golgi apparatus to bear branched poly-N-acetyl-lactosamine side chains, which become terminally sialylated upon reaching the plasma m ...
Cells Mediate Adhesion to Fibronectin, Laminin, and Collagen
Cells Mediate Adhesion to Fibronectin, Laminin, and Collagen

... types I and IV. Cell attachment to these substrates appears to be mediated by members of the f1 integrin family of extracellular matrix receptors. Antibodies to the PI subunit not only demonstrated the presence of integrin complexes in focal adhesion plaques but also blocked cell adhesion to the dif ...
HIV1 impairs in vitro priming of nave T cells and gives rise to
HIV1 impairs in vitro priming of nave T cells and gives rise to

... Treg modulate memory T-cell functions in HIV-1-infected individuals [20, 21] and their depletion could lead to immune hyperactivation. It is worth noting that chronic immune-hyperactivation associated with impaired T-cell functions is also seen in HIV-1 infection, although the underlying mechanisms ...
DOI: 10
DOI: 10

... microscope (BX 60, Olympus) equipped with a Peltier-cooled CCD camera (Hamamatsu) and analyzed with IMAGEPRO PLUS software (Media Cybernetics). The comparison between the transmission and fluorescence images of micropores shows that only the micropore walls ...
STRUCTURE-FUNCTION RELATIONSHIPS IN
STRUCTURE-FUNCTION RELATIONSHIPS IN

... from which fatty acids had been extracted as described-by Goodman '(12) Adipose cells were isolated by treating fat pads with a crude collagenase (Worthington Biochemical Corp., Freehold, N . J .) as described by Rodbell (11) with the following modifications : All procedures were carried out in poly ...
Cell Differentiation for the Regulation of Thymocyte and Th2 Distinct
Cell Differentiation for the Regulation of Thymocyte and Th2 Distinct

... GATA-3, the only T cell-specific member of the GATA family of transcription factors, is essential for the intrathymic development of CD4ⴙ T cells and for the differentiation of Th2 cells. However, whether distinct biochemical features, unique to GATA-3 compared with other GATA family members, are re ...
Regulatory role of rpL3 in cell response to nucleolar stress induced
Regulatory role of rpL3 in cell response to nucleolar stress induced

... subsequent p53 mediated cell cycle arrest or apoptosis.12 Recent findings revealed novel stress response pathways, which function independently of p53 and still lead to cell cycle arrest and/or apoptosis. Given that most cancers lack functional p53, it is of great importance to study these emerging m ...
Glutamine breakdown in rapidly dividing cells: waste or investment?
Glutamine breakdown in rapidly dividing cells: waste or investment?

... cells may require large amounts of glutamine for biosynthetic purposes, only a limited amount (<5%) of the extracted glutamine is used for such pathways.(8) However, it is noteworthy that, in rapidly dividing cells, the flux through glutaminase greatly exceeds the capacity of the mitochondria to oxi ...
Document
Document

... antibody, and data represent geometric mean fluorescence intensity (Geo. MFI) of binding. WThIgG4 indicates wild-type human SIRPα allele 2 as a fusion to human IgG4 as previously described (1). Data represent mean ± SD of three independent titration series analyzed simultaneously as one experiment. ...
Targeting Tumor Antigens to Secreted Membrane Vesicles In vivo
Targeting Tumor Antigens to Secreted Membrane Vesicles In vivo

... protein with two functional domains: NH2-terminal epidermal growth factor (EGF)–like domains, which contain an Arg-Gly-Asp sequence that binds to avh3 and avh5 integrins, and COOHterminal domains called C1 and C2, which are similar to the blood clotting factor VIII domains and which bind with strong ...
Day1 chick development
Day1 chick development

... The amniotes consist of two major groups of vertebrate animals, the synapsids (prototherian, metatherian, and eutherian mammals) and sauropsids (reptiles and birds) (Fig. 1A). Two species, the mouse representing the mammals and the chick representing the birds/reptiles, are currently used as the ben ...
PDF
PDF

... gonads To investigate whether the effects of Notch signaling on Leydig cell development are mediated through the transcriptional repressor Hes1, we analyzed Hes1–/– mouse gonads using antibodies against SOX9, PECAM1 and 3β-HSD. In both Hes1+/– and wild-type littermates, germ cells and Sertoli cells ...
Suppression of a mitotic mutant by tRNA
Suppression of a mitotic mutant by tRNA

... mitotic transition from metaphase to anaphase failed to produce colonies at 33°C, whereas the double mutants cut9665 scn1-17 and cut9-665 scn2-7 could form colonies at 33°C but not at 22°C. Single scn1 and scn2 mutants obtained as tetrad segregants after crossing with the wild-type strain also showe ...
Adult stem cells and their ability to differentiate
Adult stem cells and their ability to differentiate

... stem cells are capable of forming any tissue in the body, similarly to a fertilized egg which, following cleavage, produces cells which differentiate into all types of tissues. Pluripotency is the capability of the cell to create almost any type of cells in the organism, but not the entirety. Multip ...
Down-Regulation of DNA Topoisomerase IIα Leads to Prolonged
Down-Regulation of DNA Topoisomerase IIα Leads to Prolonged

... the transient nature of the observed cell cycle alterations. Western blot analysis indicates that vincristine-selection is accompanied by down-regulation of topoisomerase II␣ without de- ...
T Cells + Memory CD8 Influenza A Virus
T Cells + Memory CD8 Influenza A Virus

... http://www.jimmunol.org/content/178/3/1285.full#ref-list-1 Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription ...
Endomembrane trafficking protein SEC24A regulates cell size
Endomembrane trafficking protein SEC24A regulates cell size

... Size is a fundamental characteristic of a cell, but how cell size is determined is still not well understood in most living organisms (Marshall et al., 2012). Cells of different types typically have characteristic sizes, indicating that size is carefully regulated to fit cell functions during differ ...
Development of Conjunctival Goblet Cells and Their
Development of Conjunctival Goblet Cells and Their

... membranes of goblet cells.3 Although the role of the sympathetic agonists in stimulating goblet cell secretion is unknown, ␤1- and ␤2-adrenergic receptor (␤AR) subtypes appear to be present in goblet cells as well as in stratified squamous cells.6 This evidence suggests that parasympathetic and perh ...
Endomembrane trafficking protein SEC24A regulates cell size
Endomembrane trafficking protein SEC24A regulates cell size

... Size is a fundamental characteristic of a cell, but how cell size is determined is still not well understood in most living organisms (Marshall et al., 2012). Cells of different types typically have characteristic sizes, indicating that size is carefully regulated to fit cell functions during differ ...
The roles of two C. elegans HOX co-factor orthologs
The roles of two C. elegans HOX co-factor orthologs

... generated by a subset of the 12 ventral epidermal cells that are located in a row along the ventral margin of the animal (Fig. 1B) (for reviews, see Greenwald, 1997; Shemer and Podbilewicz, 2003; Wang and Sternberg, 2001). These cells, called P(1-12).p, are born during the first larval stage. Soon a ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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