Regenerated Hair Cells Can Originate from Supporting Cell Progeny
... cell divisions that give rise to hair cells and those that give rise to supporting cells are characteristic of cytogenesis in the embryonic avian cochlea, which has led to the proposal that the cells may share a single bipotent progenitor in the terminal mitosis before differentiation (Katayama and ...
... cell divisions that give rise to hair cells and those that give rise to supporting cells are characteristic of cytogenesis in the embryonic avian cochlea, which has led to the proposal that the cells may share a single bipotent progenitor in the terminal mitosis before differentiation (Katayama and ...
Cellular Maturation Defects in Bruton`s Tyrosine
... cells manifested essentially normal surface expression of these markers (Fig. 1C and data not shown). In summary, these findings indicate that in Btk-deficient mice the impaired modulation of developmentally regulated markers in small pre-B cells results in very immature phenotype of ⫹ B cells in t ...
... cells manifested essentially normal surface expression of these markers (Fig. 1C and data not shown). In summary, these findings indicate that in Btk-deficient mice the impaired modulation of developmentally regulated markers in small pre-B cells results in very immature phenotype of ⫹ B cells in t ...
Yantar, a conserved arginine-rich protein is involved in Drosophila
... of these mutations (5E24, 5K29, and 3A8) were selected because of their reproducible and fairly penetrant melanotic tumor phenotype, and we named their corresponding genes yantar (ytr), polka dots (pkdt), and splotchy (splo). Hemocytes from these mutants were visualized after immunostaining using tw ...
... of these mutations (5E24, 5K29, and 3A8) were selected because of their reproducible and fairly penetrant melanotic tumor phenotype, and we named their corresponding genes yantar (ytr), polka dots (pkdt), and splotchy (splo). Hemocytes from these mutants were visualized after immunostaining using tw ...
Non-specific chemical inhibition of the Fanconi anemia pathway
... Background: Platinum compounds such as cisplatin and carboplatin are DNA crosslinking agents widely used for cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully ...
... Background: Platinum compounds such as cisplatin and carboplatin are DNA crosslinking agents widely used for cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully ...
Glucagon receptor recycling: role of carboxyl terminus - AJP-Cell
... Fig. 1. Glucagon receptor (GR) recycles to the plasma membrane in a -arrestin-dependent manner: A: hamster hepatocytes were incubated without (control, ctrl) and with 100 nM glucagon (gluc) for 30 min (30⬘) at 37°C, washed, and then incubated or not at 37° for 120 min recycling (rec). GR expression ...
... Fig. 1. Glucagon receptor (GR) recycles to the plasma membrane in a -arrestin-dependent manner: A: hamster hepatocytes were incubated without (control, ctrl) and with 100 nM glucagon (gluc) for 30 min (30⬘) at 37°C, washed, and then incubated or not at 37° for 120 min recycling (rec). GR expression ...
Project Details - School of Biomedical Sciences
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
Sorting between the ER and Golgi
... particular, ts-045-G has been widely used as a model protein for these studies, as it can be accumulated within the ER at 39.5°C and released as a relatively synchronous wave of transport at the permissive temperature 31°C (LippincottSchwartz et al., 2000; Stephens et al., 2000; Scales et al., 1997; ...
... particular, ts-045-G has been widely used as a model protein for these studies, as it can be accumulated within the ER at 39.5°C and released as a relatively synchronous wave of transport at the permissive temperature 31°C (LippincottSchwartz et al., 2000; Stephens et al., 2000; Scales et al., 1997; ...
The Science of Pressure Ulcer Development
... the problem, we must realize that tissues temporarily deprived of their blood supply do not always achieve complete restoration of blood flow when reconnected to a normal supply (22, 23). It is also known that during ischemia, an enzymatic conversion of xanthine dehydrogenase to xanthine oxidase oc ...
... the problem, we must realize that tissues temporarily deprived of their blood supply do not always achieve complete restoration of blood flow when reconnected to a normal supply (22, 23). It is also known that during ischemia, an enzymatic conversion of xanthine dehydrogenase to xanthine oxidase oc ...
Second Harmonic Imaging of Plant Polysaccharides
... detailed image was obtained but it is not easy to interpret; a more sophisticated preparation technique may be needed to take this approach further. Starch pastes and doughs of one sort or another are fundamental in the food-processing industry and Figure 6b shows a thin layer of rice-flour paste mo ...
... detailed image was obtained but it is not easy to interpret; a more sophisticated preparation technique may be needed to take this approach further. Starch pastes and doughs of one sort or another are fundamental in the food-processing industry and Figure 6b shows a thin layer of rice-flour paste mo ...
Programmed Cell Death in Floral Organs: How and Why do Flowers
... of sunflower, there is cytochrome c release into the cytosol followed by changes in cell morphology, loss of outer mitochondrial membrane integrity and a fall in the respiratory control ratio (Balk and Leaver, 2001). Assuming that CMS is just anticipating a normal event (quite a major assumption), i ...
... of sunflower, there is cytochrome c release into the cytosol followed by changes in cell morphology, loss of outer mitochondrial membrane integrity and a fall in the respiratory control ratio (Balk and Leaver, 2001). Assuming that CMS is just anticipating a normal event (quite a major assumption), i ...
Expression of Recombinant Myelin
... (31). After a further 48 h, G418 (200 ~tg/ml final effective concentration) was added; and 14-21 d later, small groups of resistant cells were observed; in general 1-2 colonies were obtained per coverslip. Forskolin and glia growth factor were removed 21-28 d post infection to avoid the onset of unr ...
... (31). After a further 48 h, G418 (200 ~tg/ml final effective concentration) was added; and 14-21 d later, small groups of resistant cells were observed; in general 1-2 colonies were obtained per coverslip. Forskolin and glia growth factor were removed 21-28 d post infection to avoid the onset of unr ...
Spontaneous zygogenesis in Escherichia coli, a form
... the selected colony were suspended in 10 ml M9 and these suspensions were strongly vortexed for 30 s. Furthermore, samples were streaked onto the medium used in the initial selection. Two to three colonies per isolated clone were picked up and incubated for 90 min in LB. Cells were allowed to divide ...
... the selected colony were suspended in 10 ml M9 and these suspensions were strongly vortexed for 30 s. Furthermore, samples were streaked onto the medium used in the initial selection. Two to three colonies per isolated clone were picked up and incubated for 90 min in LB. Cells were allowed to divide ...
AWT ® Acoustic Wave Therapy
... Acoustic waves are sound waves that are characterized by high pressure amplitudes in comparison to the ambient pressure. Sound waves used for medical applications are generated extracorporeally and introduced into the body without causing skin lesions. Owing to the extraordinary success achieved in ...
... Acoustic waves are sound waves that are characterized by high pressure amplitudes in comparison to the ambient pressure. Sound waves used for medical applications are generated extracorporeally and introduced into the body without causing skin lesions. Owing to the extraordinary success achieved in ...
Transcriptional insights into the CD8+ T cell response to infection
... visualize changes in gene expression over the course of the CD8 + T cell response to infection with Lm-OVA, we selected 7,195 genes that had a difference in expression of at least twofold in any two samples in the data set from the total of 25,194 genes examined (Fig. 1b and Supplementary Table 1). ...
... visualize changes in gene expression over the course of the CD8 + T cell response to infection with Lm-OVA, we selected 7,195 genes that had a difference in expression of at least twofold in any two samples in the data set from the total of 25,194 genes examined (Fig. 1b and Supplementary Table 1). ...
Oxidation of Hg(0) - Department of Marine and Coastal Sciences
... Initial Hg(0) oxidation experiments were conducted with live, nongrowing, resting cells. Bacterial cultures were centrifuged at 10,000 RPM for 7 min and the cells were washed in 0.5 mM anoxic phosphate buffer (0.38 mM Na2HPO4 and 0.12 mM NaH2PO4) containing 1 mM NaCl inside an anaerobic glove box un ...
... Initial Hg(0) oxidation experiments were conducted with live, nongrowing, resting cells. Bacterial cultures were centrifuged at 10,000 RPM for 7 min and the cells were washed in 0.5 mM anoxic phosphate buffer (0.38 mM Na2HPO4 and 0.12 mM NaH2PO4) containing 1 mM NaCl inside an anaerobic glove box un ...
Development of definitive endoderm from embryonic
... based on findings from studies using model systems such as Xenopus and zebrafish that provide easy access to early embryonic stages of development at a time when lineage commitment decisions are taking place. By contrast, the mouse embryo is much less amenable to such experimental approaches because ...
... based on findings from studies using model systems such as Xenopus and zebrafish that provide easy access to early embryonic stages of development at a time when lineage commitment decisions are taking place. By contrast, the mouse embryo is much less amenable to such experimental approaches because ...
Paclitaxel-induced microtubule stabilization causes mitotic block
... cycle (Gupta et al., 2002). Thus, to affect cell proliferation, the paclitaxel concentration in the cell might have to be increased to such a level as to completely shut down microtubule dynamics. Apoptosis, a natural process of programmed cell death that is required for the proper development of mu ...
... cycle (Gupta et al., 2002). Thus, to affect cell proliferation, the paclitaxel concentration in the cell might have to be increased to such a level as to completely shut down microtubule dynamics. Apoptosis, a natural process of programmed cell death that is required for the proper development of mu ...
OPINION N° 117 Use of stem cells derived from umbilical cord blood
... medullary cells were injected to patients with medullary aplasia after they had undergone accidental and potentially lethal civilian nuclear irradiation. This therapeutic trial, which was published in scientific journals, showed that haematopoietic bone marrow transplantation was technically feasibl ...
... medullary cells were injected to patients with medullary aplasia after they had undergone accidental and potentially lethal civilian nuclear irradiation. This therapeutic trial, which was published in scientific journals, showed that haematopoietic bone marrow transplantation was technically feasibl ...
Phospholamban Is Present in Endothelial Cells and Modulates
... skeletal and smooth muscle in which PLB is an important regulator of [Ca21]i and contractility in these muscles. In the present study, we report the existence of PLB in the vascular endothelium, a nonmuscle tissue, and provide functional data on PLB regulation of vascular contractility through its a ...
... skeletal and smooth muscle in which PLB is an important regulator of [Ca21]i and contractility in these muscles. In the present study, we report the existence of PLB in the vascular endothelium, a nonmuscle tissue, and provide functional data on PLB regulation of vascular contractility through its a ...
The posterior section of the chick`s area pellucida
... expanded both anteriorly and laterally as compared to stage 2, its shape however remained very similar to the latter. Also in the epiblast the same pattern was found as in stage 2, namely, the PS alone was entirely composed of lablled cells, while the epiblastic cells surrounding it were not labelle ...
... expanded both anteriorly and laterally as compared to stage 2, its shape however remained very similar to the latter. Also in the epiblast the same pattern was found as in stage 2, namely, the PS alone was entirely composed of lablled cells, while the epiblastic cells surrounding it were not labelle ...
Stem Cell or Bone Marrow Donation
... factor injections, can harm a developing baby (embryo or fetus). You should not take filgrastim if you are pregnant. All female donors who have the potential to become pregnant will be given a pregnancy test before being accepted as a donor. If you are pregnant, you will not be allowed to donate. Yo ...
... factor injections, can harm a developing baby (embryo or fetus). You should not take filgrastim if you are pregnant. All female donors who have the potential to become pregnant will be given a pregnancy test before being accepted as a donor. If you are pregnant, you will not be allowed to donate. Yo ...
Biology of the Cell
... have more surface area per unit of volume than large ones, the control is more effective when cells are relatively small. Although most cells are small, some cells are nonetheless quite large and have apparently overcome the surface areato-volume problem by one or more adaptive mechanisms. For examp ...
... have more surface area per unit of volume than large ones, the control is more effective when cells are relatively small. Although most cells are small, some cells are nonetheless quite large and have apparently overcome the surface areato-volume problem by one or more adaptive mechanisms. For examp ...
Ectodermal progenitors derived from epiblast
... In order to investigate the developmental potential of ESDEpiSCs by Nodal inhibition, ESD-EpiSCs were differentiated under four conditions, CDM only (Control), CDM with BMP4 (BMP4), CDM with SB431542 (SB43), and CDM with BMP4 plus SB43 (BMP4/ SB43), for 3 days. Intriguingly, cells cultured under dif ...
... In order to investigate the developmental potential of ESDEpiSCs by Nodal inhibition, ESD-EpiSCs were differentiated under four conditions, CDM only (Control), CDM with BMP4 (BMP4), CDM with SB431542 (SB43), and CDM with BMP4 plus SB43 (BMP4/ SB43), for 3 days. Intriguingly, cells cultured under dif ...
Calibrating Thermometers
... As an example of a comparison calibration let us examine two ways of calibrating an industrial platinum resistance thermometer (IPRT) at 150° C. This is best achieved using a stirred oil bath and a standard platinum resistance thermometer (SPRT). For the best results the two thermometers would be mo ...
... As an example of a comparison calibration let us examine two ways of calibrating an industrial platinum resistance thermometer (IPRT) at 150° C. This is best achieved using a stirred oil bath and a standard platinum resistance thermometer (SPRT). For the best results the two thermometers would be mo ...
Bone physiology
... Fig. 1-81 illustrates an osteon with osteocytes (OC) residing in osteocyte lacunae in the lamellar bone. The osteocytes connect via canaliculi (can) which contain cytoplasmatic projections of the osteocytes. A Haversian canal (HC) is seen in the middle of the osteon. Fig. 1-82 illustrates an area ...
... Fig. 1-81 illustrates an osteon with osteocytes (OC) residing in osteocyte lacunae in the lamellar bone. The osteocytes connect via canaliculi (can) which contain cytoplasmatic projections of the osteocytes. A Haversian canal (HC) is seen in the middle of the osteon. Fig. 1-82 illustrates an area ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.