collection fact sheet
... cartilage. HA not only helps keep the cartilage that cushions joints strong and flexible, but also helps increase supplies of joint-lubricating synovial fluid. Hyaluronic acid is present in every tissue of the body, and it performs many important functions. It helps deliver nutrients to and carry to ...
... cartilage. HA not only helps keep the cartilage that cushions joints strong and flexible, but also helps increase supplies of joint-lubricating synovial fluid. Hyaluronic acid is present in every tissue of the body, and it performs many important functions. It helps deliver nutrients to and carry to ...
Polypeptide Composition of Chlamydia trachomatis
... confluent, DEAE-dextran-treated HeLa 229 cell monolayers on 13 mm diameter coverslips. The monolayers were incubated to permit inclusion development, stained with Hoechst 3 3258 and the numbers of inclusions were counted (Salari & Ward, 1979). The dilution of yolk sac-derived chlamydiae capable of i ...
... confluent, DEAE-dextran-treated HeLa 229 cell monolayers on 13 mm diameter coverslips. The monolayers were incubated to permit inclusion development, stained with Hoechst 3 3258 and the numbers of inclusions were counted (Salari & Ward, 1979). The dilution of yolk sac-derived chlamydiae capable of i ...
COMPOUND-INDUCED HOST CELL RESPONSES AND EFFECTS ON WHOLE VIRUS CHAPTER 4
... clinical trials (Hamid et al., 2004) with toxicity and adverse effects being some of the reasons for the failures. While the in silico prediction models (covered in chapter 3) provides insights into potential toxicity and can significantly shorten the drug discovery time line (Lobell and Sivarajah, ...
... clinical trials (Hamid et al., 2004) with toxicity and adverse effects being some of the reasons for the failures. While the in silico prediction models (covered in chapter 3) provides insights into potential toxicity and can significantly shorten the drug discovery time line (Lobell and Sivarajah, ...
An ARL1 mutation affected autophagic cell death in yeast
... after about 48 h. We have previously referred to this phenomenon as autophagic death. In the present study, we isolated and characterized a recessive mutant strain, dlp2, which delays the progression toward autophagic death. The cdc28 dlp2 cells contain many small vesicles instead of the large centr ...
... after about 48 h. We have previously referred to this phenomenon as autophagic death. In the present study, we isolated and characterized a recessive mutant strain, dlp2, which delays the progression toward autophagic death. The cdc28 dlp2 cells contain many small vesicles instead of the large centr ...
Cell Structure Tumor Microenvironment
... • Cellular protein quality control by extracting and degrading unfolded proteins (known as a ER-associated protein degradation-ERAD) • Lipid and sterol biosynthesis • Storage of calcium ions in the ER lumen and their regulated release into the cytosol (calcium homeostasis) • Detoxification of drugs ...
... • Cellular protein quality control by extracting and degrading unfolded proteins (known as a ER-associated protein degradation-ERAD) • Lipid and sterol biosynthesis • Storage of calcium ions in the ER lumen and their regulated release into the cytosol (calcium homeostasis) • Detoxification of drugs ...
Control of Mitotic Events by Nap1 and the Gin4 Kinase
... the bud begins to grow over its entire surface. (Lew and Reed, 1993, 1995). Cells that lack Clb function fail to make this switch and continue polar bud growth during mitosis, giving rise to highly elongated buds (Amon et al., 1993; Lew and Reed, 1993; Richardson et al., 1992). Nap1 is required for ...
... the bud begins to grow over its entire surface. (Lew and Reed, 1993, 1995). Cells that lack Clb function fail to make this switch and continue polar bud growth during mitosis, giving rise to highly elongated buds (Amon et al., 1993; Lew and Reed, 1993; Richardson et al., 1992). Nap1 is required for ...
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... experimental assays. These DCMECs exhibited the typical cobblestone-like morphology that differed significantly from fibroblasts; we also found the expression of the epithelial cell-specific keratin CK18. Furthermore, analysis of the lactating ability of DCMECs revealed that these DCMECs can synthes ...
... experimental assays. These DCMECs exhibited the typical cobblestone-like morphology that differed significantly from fibroblasts; we also found the expression of the epithelial cell-specific keratin CK18. Furthermore, analysis of the lactating ability of DCMECs revealed that these DCMECs can synthes ...
Dominant negative connexin26 mutation R75W causing severe
... the tunnel of Corti collapsed [6]. In this regard, the cochleae of R75W + mice were quite similar to those of caspase 3 (−/−) mice [32], in which the space that forms the tunnel of Corti is known to collapse, reducing the height of the organ of Corti. We have previously shown GER retention in mice t ...
... the tunnel of Corti collapsed [6]. In this regard, the cochleae of R75W + mice were quite similar to those of caspase 3 (−/−) mice [32], in which the space that forms the tunnel of Corti is known to collapse, reducing the height of the organ of Corti. We have previously shown GER retention in mice t ...
Context-Dependent Synaptic Action of Glycinergic and GABAergic
... 7.4. Parasagittal slices 200-350 pm thick were prepared using an oscillating tissue slicer (Frederick Haer, New Brunswick, ME). Slices were maintained in a chamber where they were submcrgcd in rapidly flowing, oxygenated saline solution at -34°C. Flow rates through the 0.3 ml chamber ranged from 9 t ...
... 7.4. Parasagittal slices 200-350 pm thick were prepared using an oscillating tissue slicer (Frederick Haer, New Brunswick, ME). Slices were maintained in a chamber where they were submcrgcd in rapidly flowing, oxygenated saline solution at -34°C. Flow rates through the 0.3 ml chamber ranged from 9 t ...
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... Hematodinium from the Norway lobster versus the blue crab (Table 2). Strong positive reactions for several enzymes were observed for the control Hematodinium-free culture medium which prevented them from being assayed in the Hematodinium cell culture media. Marked increases in enzyme activity betwee ...
... Hematodinium from the Norway lobster versus the blue crab (Table 2). Strong positive reactions for several enzymes were observed for the control Hematodinium-free culture medium which prevented them from being assayed in the Hematodinium cell culture media. Marked increases in enzyme activity betwee ...
Memoryless self-reinforcing directionality in endosomal active
... actual turns, we defined ‘flights’, which are consecutive runs that persist in direction punctuated by pauses (Fig. 2c), and identified the turning points between flights, employing to do so the errorradius analysis standard in the field of ecology7 (Methods and Supplementary Figs 2 and 3). We find ...
... actual turns, we defined ‘flights’, which are consecutive runs that persist in direction punctuated by pauses (Fig. 2c), and identified the turning points between flights, employing to do so the errorradius analysis standard in the field of ecology7 (Methods and Supplementary Figs 2 and 3). We find ...
TESIS DOCTORAL
... SUMMARY The irreversible necrosis of heart muscle (myocardial infarction, MI) occurs when ischemia exceeds a critical threshold and overwhelms myocardial cellular repair mechanisms. After MI, myocardial tissue lacks the ability to significantly regenerate itself and as a consequence, ischemia might ...
... SUMMARY The irreversible necrosis of heart muscle (myocardial infarction, MI) occurs when ischemia exceeds a critical threshold and overwhelms myocardial cellular repair mechanisms. After MI, myocardial tissue lacks the ability to significantly regenerate itself and as a consequence, ischemia might ...
The Nhal antiporter of Saccharomyces cerevisiae mediates sodium
... Peiia & Ramirez, 1991; Camarasa et al., 1996; Ramirez et al., 1996), but the gene(s) responsible has not yet been identified. Finally, it must be noted that the ENAl gene involved in Na+efflux (see below) could also be involved in K+ efflux, as has been suggested after characterization of enal-4 mut ...
... Peiia & Ramirez, 1991; Camarasa et al., 1996; Ramirez et al., 1996), but the gene(s) responsible has not yet been identified. Finally, it must be noted that the ENAl gene involved in Na+efflux (see below) could also be involved in K+ efflux, as has been suggested after characterization of enal-4 mut ...
Antioxidant defense system targeted to help maintain a healthy
... Alpha-lipoic acid is able to cross the blood-brain barrier and functions in fat or water. In fact, it interacts with its antioxidant partners — vitamins E and C — and also helps to conserve them. When the body is deficient of glutathione and lipoic acid, the other antioxidants do not properly netwo ...
... Alpha-lipoic acid is able to cross the blood-brain barrier and functions in fat or water. In fact, it interacts with its antioxidant partners — vitamins E and C — and also helps to conserve them. When the body is deficient of glutathione and lipoic acid, the other antioxidants do not properly netwo ...
video slide - Issaquah Connect
... Light microscopy enables study of living cells and may introduce fewer artifacts than do TEM and SEM ...
... Light microscopy enables study of living cells and may introduce fewer artifacts than do TEM and SEM ...
Chapter 22: The Living Cell - Follow “Ironmtn.wordpress.com”
... 58. At the cellular and molecular level, in what key ways are plants and animals the same? In what essential ways are they different? How do these differences give the organism an advantage in its life strategies? Ans: Cellular respiration is the same; plants have chloroplasts, which allows them to ...
... 58. At the cellular and molecular level, in what key ways are plants and animals the same? In what essential ways are they different? How do these differences give the organism an advantage in its life strategies? Ans: Cellular respiration is the same; plants have chloroplasts, which allows them to ...
Self-Assembled Monolayers That Resist the Adsorption of Proteins
... positively charged under the experimental conditions used, and it is often used as a model protein in studies of electrostatic adsorption.65,66 We chose to test the SAMs against the adhesion of S. aureus and S. epidermidis because these organisms cause 30-50% of infections due to indwelling devices. ...
... positively charged under the experimental conditions used, and it is often used as a model protein in studies of electrostatic adsorption.65,66 We chose to test the SAMs against the adhesion of S. aureus and S. epidermidis because these organisms cause 30-50% of infections due to indwelling devices. ...
Gepstein Oren Caspi, Irit Huber, Amira Gepstein, Gil Arbel, Leonid
... arrhythmias and sudden cardiac death. We aimed to establish a patient-/disease-specific human induced pluripotent stem cell (hiPSC) model of ARVC. Methods and Results—Dermal fibroblasts were obtained from 2 patients with ARVC with plakophilin-2 (PKP2) mutations, reprogrammed to generate hiPSCs, coax ...
... arrhythmias and sudden cardiac death. We aimed to establish a patient-/disease-specific human induced pluripotent stem cell (hiPSC) model of ARVC. Methods and Results—Dermal fibroblasts were obtained from 2 patients with ARVC with plakophilin-2 (PKP2) mutations, reprogrammed to generate hiPSCs, coax ...
Chapter 4 Test Cell Structure Answer Section
... homeostasis by moving supplies from one part of the cell to the other is the a. cytoplasm. b. mitochondrion. c. nucleus. d. endoplasmic reticulum. 37. Plant cells a. have a cell wall instead of a plasma membrane. b. have chloroplasts and a cell wall. c. do not contain mitochondria. d. have a large c ...
... homeostasis by moving supplies from one part of the cell to the other is the a. cytoplasm. b. mitochondrion. c. nucleus. d. endoplasmic reticulum. 37. Plant cells a. have a cell wall instead of a plasma membrane. b. have chloroplasts and a cell wall. c. do not contain mitochondria. d. have a large c ...
BioWire_Progress_Report_Week_One_Rev_1
... [BioBrick and AHL should arrive by Tuesday] Manually add AHL to the system in varying concentrations Control: Add water instead of AHL BioBrick comes with YFP as a reporter Expected Result: YFP is expressed, but not in control plates. Note: replicate experiment using GFP/mCherry as decided ...
... [BioBrick and AHL should arrive by Tuesday] Manually add AHL to the system in varying concentrations Control: Add water instead of AHL BioBrick comes with YFP as a reporter Expected Result: YFP is expressed, but not in control plates. Note: replicate experiment using GFP/mCherry as decided ...
2010 - Department of Biology
... Wilson 1990, 2008). Workers in the majority of ant species have retained their ovaries, but do not have ...
... Wilson 1990, 2008). Workers in the majority of ant species have retained their ovaries, but do not have ...
Stem cells in cancer: instigators and propagators?
... generated in the study in which Apc was deleted from the Lgr5expressing population. Intestinal tumours may also be initiated from the stem cell population at positions 4-5 above the base of the crypt. These cells overexpress Wip1 phosphatase, an enzyme that turns off the DNA-damage response through ...
... generated in the study in which Apc was deleted from the Lgr5expressing population. Intestinal tumours may also be initiated from the stem cell population at positions 4-5 above the base of the crypt. These cells overexpress Wip1 phosphatase, an enzyme that turns off the DNA-damage response through ...
Microtechnologies for Cell Microenvironment Control and
... behavior, by biophysical, biochemical or other pathways. As previously stated, those factors can be classified in: ECM, cells surrounding a single cell, soluble factors, topography or physical properties of classified in: ECM, cells surrounding a single cell, soluble factors, topography or physical ...
... behavior, by biophysical, biochemical or other pathways. As previously stated, those factors can be classified in: ECM, cells surrounding a single cell, soluble factors, topography or physical properties of classified in: ECM, cells surrounding a single cell, soluble factors, topography or physical ...
3. The Sea Urchin
... Lepage; Lepage et at., 1992). The boundary of expression sharply demarcates future ectoderm from endoderm and mesoderm. 3.26 (x420) shows an in situ hybridization of a Lytechinus variegatus embryo using an anti-sense probe for LvS1 (Wessell et at., 1987), a member of the Spec 1 family of genes (Lynn ...
... Lepage; Lepage et at., 1992). The boundary of expression sharply demarcates future ectoderm from endoderm and mesoderm. 3.26 (x420) shows an in situ hybridization of a Lytechinus variegatus embryo using an anti-sense probe for LvS1 (Wessell et at., 1987), a member of the Spec 1 family of genes (Lynn ...
The AMF-R tubule is a smooth ilimaquinone
... and Tac-TGN38 in red) show that the AMF-R and TGN labels can be distinguished. This is particulary evident in the cell to the right which exhibits a more spread morphology. As described for the IQ-mediated breakdown of Golgi membranes, IQ does not affect AMF-R tubule morphology when cells are treate ...
... and Tac-TGN38 in red) show that the AMF-R and TGN labels can be distinguished. This is particulary evident in the cell to the right which exhibits a more spread morphology. As described for the IQ-mediated breakdown of Golgi membranes, IQ does not affect AMF-R tubule morphology when cells are treate ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.